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The map label for this gene is smpB [H]

Identifier: 170025202

GI number: 170025202

Start: 3279327

End: 3279821

Strand: Direct

Name: smpB [H]

Synonym: YPK_2980

Alternate gene names: 170025202

Gene position: 3279327-3279821 (Clockwise)

Preceding gene: 170025199

Following gene: 170025203

Centisome position: 69.93

GC content: 45.86

Gene sequence:

>495_bases
ATGACCGATACTATGACAAAGAAAAAAGCATACAAACCCGGTTCCGCAACCATTGCGCAAAATAAACGAGCCCGTCACGA
ATACTTCATTGAAGAAGAGTTCGAAGCTGGGCTTGCACTGCAAGGTTGGGAAGTAAAATCACTGCGTGCAGGCAAAGCAA
ATATCAGTGACAGCTACGTAATGTTTAAAAACGGTGAGGCATTCCTGTTTGGGGCCACAATTACGCCGTTGAACGTTGCG
TCGACTCATGTTGTTTGCGAGCCTATGCGTACCCGTAAACTGCTGTTGAACAAACGCGAACTGGATTCCTTGTTTGGCCG
AGTGAACCGAGAAGGCTACACGGTTGTCGCTCTCTCCATGTATTGGAAAAATGCCTGGGTGAAAGTTAAAATCGGCGTAG
CAAAAGGTAAAAAAGATAACGACAAGCGCGATGATATTAGAGATCGTGAGTGGAAGTTAGACAAAGCGCGCATCATGAAG
CACGCAAATCGTTAA

Upstream 100 bases:

>100_bases
CGCCAGACAAAAAATCTGCCAGGCATTCCTCGCAACAAATGAGACAAAATACATAAGCATCTTACGGGAAAGGATTTCAT
TCGCCTCCGCATACAGTATA

Downstream 100 bases:

>100_bases
GTAACTGGCATCGCAGGACATAGTTCTGTTATACTAGCTGAGTTCTTTGGGGCTGATTCTGGATTCGACGGGATTCGCGA
AACCCAAGGTGCATGCCGAG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTDTMTKKKAYKPGSATIAQNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANISDSYVMFKNGEAFLFGATITPLNVA
STHVVCEPMRTRKLLLNKRELDSLFGRVNREGYTVVALSMYWKNAWVKVKIGVAKGKKDNDKRDDIRDREWKLDKARIMK
HANR

Sequences:

>Translated_164_residues
MTDTMTKKKAYKPGSATIAQNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANISDSYVMFKNGEAFLFGATITPLNVA
STHVVCEPMRTRKLLLNKRELDSLFGRVNREGYTVVALSMYWKNAWVKVKIGVAKGKKDNDKRDDIRDREWKLDKARIMK
HANR
>Mature_163_residues
TDTMTKKKAYKPGSATIAQNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANISDSYVMFKNGEAFLFGATITPLNVAS
THVVCEPMRTRKLLLNKRELDSLFGRVNREGYTVVALSMYWKNAWVKVKIGVAKGKKDNDKRDDIRDREWKLDKARIMKH
ANR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=85, Blast_Score=289, Evalue=7e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18856; Mature: 18725

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTMTKKKAYKPGSATIAQNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANISDSYV
CCCCCCCHHCCCCCCCCHHCCCCCHHHHCCHHHHCCCCEECCCEECEEECCCCCCCCCEE
MFKNGEAFLFGATITPLNVASTHVVCEPMRTRKLLLNKRELDSLFGRVNREGYTVVALSM
EEECCCEEEEEEEECCCCCCCCEEEECCHHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEE
YWKNAWVKVKIGVAKGKKDNDKRDDIRDREWKLDKARIMKHANR
EECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TDTMTKKKAYKPGSATIAQNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANISDSYV
CCCCCCHHCCCCCCCCHHCCCCCHHHHCCHHHHCCCCEECCCEECEEECCCCCCCCCEE
MFKNGEAFLFGATITPLNVASTHVVCEPMRTRKLLLNKRELDSLFGRVNREGYTVVALSM
EEECCCEEEEEEEECCCCCCCCEEEECCHHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEE
YWKNAWVKVKIGVAKGKKDNDKRDDIRDREWKLDKARIMKHANR
EECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA