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The map label for this gene is rumB

Identifier: 170024950

GI number: 170024950

Start: 3005541

End: 3006671

Strand: Reverse

Name: rumB

Synonym: YPK_2725

Alternate gene names: 170024950

Gene position: 3006671-3005541 (Counterclockwise)

Preceding gene: 170024951

Following gene: 170024948

Centisome position: 64.12

GC content: 52.25

Gene sequence:

>1131_bases
ATGCATTGCGCACAGTATGCGGCAGGTCGCTGCCGTTCTTGTCAGTGGTTGGATAAACCCTATCCACAACAACTCGCTGA
TAAACAGCATCATCTGGAAAGCCTGTTGGCTGGGCATGCGGTCACTCAGTGGCTAGCACTGGTTTTTGGGCGCGAAAGTG
CCTTTCGTAACAAGGCGAAAATGGTCGTCAGCGGCAGTGTGGAACGCCCGTTACTGGGTATGCTGCATCGTGACGGTACA
CCGGTTGATTTATGCGCATGTCCACTTTATCCGCCCAGCTTCGAACCGGTATTTACGGTACTGAAAACCTTTATTGCCAG
GGCGGGTTTGACTCCCTATAACGTTGCTCGCAAGCGTGGCGAACTTAAATTCCTGTTACTCACGGAAAGTACCTACAACG
GTGAATTGATGCTGCGTTTTGTGCTGCGTTCTGAAACTAAATTAGCGCAGTTAACTGCTGCGTTGCCGTGGCTGCAACAA
CAGTTGCCGCAGTTGGCGGTGATCTCGGCTAATATTCAGCCAGTGCATATGGCCATTCTGGAAGGGGAGCGGGAGATCCC
GCTGACCGAACAACAGGCCCTGCCTGAGCGGTTTAATCAGGTGCCGTTGTATATCCGCCCACAAAGTTTTTTCCAGACCA
ATCCACCGGTGGCGGCTTCGTTGTATGCAACAGCACGGCAGTGGGTGCAGGAGCATGAGGTTCACAGTATGTGGGATCTG
TTCTGTGGTGTGGGCGGCTTTGGTTTACATTGTGCGGGGCCAGAGACTCAATTGACCGGTATTGAAATCAGTGCTGAAGC
TATCGCCTGTGCCCGCCAGTCGGCTGAGCAGTTAGGGCTAAAAAATGTCAGTTTCGCCGCGCTGGATTCTACCCGCTTTG
CTACCGCTGAAGCCCAAATACCTGAACTGGTCTTGGTGAATCCACCACGGCGGGGGATCGGCCGCGAGTTATGTGATTAC
CTGAGCCAGATGGCACCTAAATTTATTCTCTATTCAAGTTGTAATGCAGAGACGATGGCGAAAGATATCAGTTTGCTTGC
GGGTTACCACATTGAACGGGTACAGCTGTTTGATATGTTCCCGCATACCAGTCACTACGAAGTGCTCACCTTGCTGGCTC
TCCGTCGCTAG

Upstream 100 bases:

>100_bases
CAAAAATGAGTGTTTAACGCCATAGGGTGCTACAATCCGCGCCCATCGACAGCTTCGGCTGCACATTATCTACTGTCTCA
GAGTATAACGGTCAATATTA

Downstream 100 bases:

>100_bases
ACGTGGTTTGTGGCTTAAGTGAGTATGCAGTGAGCCAAAGTCAAAGCGTTAAAACGTTAAAGTCACTGGCGTTGCAGGTC
GGCAGCTAACAAGCGCATCC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MHCAQYAAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLALVFGRESAFRNKAKMVVSGSVERPLLGMLHRDGT
PVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRGELKFLLLTESTYNGELMLRFVLRSETKLAQLTAALPWLQQ
QLPQLAVISANIQPVHMAILEGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPPVAASLYATARQWVQEHEVHSMWDL
FCGVGGFGLHCAGPETQLTGIEISAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQIPELVLVNPPRRGIGRELCDY
LSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMFPHTSHYEVLTLLALRR

Sequences:

>Translated_376_residues
MHCAQYAAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLALVFGRESAFRNKAKMVVSGSVERPLLGMLHRDGT
PVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRGELKFLLLTESTYNGELMLRFVLRSETKLAQLTAALPWLQQ
QLPQLAVISANIQPVHMAILEGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPPVAASLYATARQWVQEHEVHSMWDL
FCGVGGFGLHCAGPETQLTGIEISAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQIPELVLVNPPRRGIGRELCDY
LSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMFPHTSHYEVLTLLALRR
>Mature_376_residues
MHCAQYAAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLALVFGRESAFRNKAKMVVSGSVERPLLGMLHRDGT
PVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRGELKFLLLTESTYNGELMLRFVLRSETKLAQLTAALPWLQQ
QLPQLAVISANIQPVHMAILEGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPPVAASLYATARQWVQEHEVHSMWDL
FCGVGGFGLHCAGPETQLTGIEISAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQIPELVLVNPPRRGIGRELCDY
LSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMFPHTSHYEVLTLLALRR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=226, Percent_Identity=27.8761061946903, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI51173878, Length=226, Percent_Identity=27.8761061946903, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1787083, Length=372, Percent_Identity=74.4623655913979, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1789148, Length=374, Percent_Identity=26.2032085561497, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790403, Length=156, Percent_Identity=30.1282051282051, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24666579, Length=414, Percent_Identity=24.6376811594203, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMB_YERP3 (A7FK27)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001401605.1
- ProteinModelPortal:   A7FK27
- SMR:   A7FK27
- STRING:   A7FK27
- GeneID:   5385608
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_2640
- NMPDR:   fig|349747.3.peg.4059
- eggNOG:   COG2265
- HOGENOM:   HBG296593
- OMA:   QPQHAAI
- ProtClustDB:   PRK03522
- BioCyc:   YPSE349747:YPSIP31758_2640-MONOMER
- HAMAP:   MF_01012
- InterPro:   IPR011825
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02085

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.189

Molecular weight: Translated: 42088; Mature: 42088

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 334-334 BINDING 212-212 BINDING 241-241 BINDING 262-262 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHCAQYAAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLALVFGRESAFRNKAK
CCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHE
MVVSGSVERPLLGMLHRDGTPVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
ELKFLLLTESTYNGELMLRFVLRSETKLAQLTAALPWLQQQLPQLAVISANIQPVHMAIL
CEEEEEEECCCCCCHHEEEEEHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEE
EGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPPVAASLYATARQWVQEHEVHSMWDL
CCCCCCCCCHHHHHHHHHCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FCGVGGFGLHCAGPETQLTGIEISAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQI
HHCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCC
PELVLVNPPRRGIGRELCDYLSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMF
CEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCHHEEEEECCC
PHTSHYEVLTLLALRR
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHCAQYAAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLALVFGRESAFRNKAK
CCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHE
MVVSGSVERPLLGMLHRDGTPVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
ELKFLLLTESTYNGELMLRFVLRSETKLAQLTAALPWLQQQLPQLAVISANIQPVHMAIL
CEEEEEEECCCCCCHHEEEEEHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEE
EGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPPVAASLYATARQWVQEHEVHSMWDL
CCCCCCCCCHHHHHHHHHCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FCGVGGFGLHCAGPETQLTGIEISAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQI
HHCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCC
PELVLVNPPRRGIGRELCDYLSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMF
CEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCHHEEEEECCC
PHTSHYEVLTLLALRR
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA