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The map label for this gene is pksG [H]

Identifier: 170024833

GI number: 170024833

Start: 2861462

End: 2862700

Strand: Reverse

Name: pksG [H]

Synonym: YPK_2608

Alternate gene names: 170024833

Gene position: 2862700-2861462 (Counterclockwise)

Preceding gene: 170024834

Following gene: 170024832

Centisome position: 61.05

GC content: 44.31

Gene sequence:

>1239_bases
ATGAATAGTATTGGTATTGAAAAAATCAGTATCTACCCAAGTAGTTTGGCGCTGAAATTATCTGAATTGGCACAAGCACG
TGGTGGGGACAATAAATACATTCAAGAATCTTTAATGGTCATAAAGCGCAGTTTGGTACCACAGTGGGAAGATGCGGTCA
CCCTTGCCGTCAATAGTGCCAAGGATATGCTTAACGATCAGGATCGTTCGGATATCGGTTTATTTATCATTGCAACAGAA
TCAGGTTTAGACCAAGAAAAGGCCATCAGTACTTGGGCGCATCGTTATTTAGGATTACCTGCGACTTGCCGTGTTTTTGA
ACTGAAATCTGCCTGCCATGCTGGTGCTGCGGCATTAAAGATGGCTGAGTCATGGATATCGTCCGGCCTTAATCGAGGGA
AAAAGGTATTACTGATCAACGTGGATATCAGTAATAATATGATTTGCCACGGGAAGATGGAATATAGCCTTGGTGCTGGG
GCCATTGCTTTACTTATCTCAGATAATCCCGTTTTTGCCGTTATTGAAACAGGGAAATCGGGTATTCATACTCATGAAGT
GACGGATGTTATTCGCCCACTTCCCTGGAAAGAGGTCGCTTTCGATGTAGAAGCCAGCCTTTTCTCTTATATGGATGGAC
TCGAGAATGCTTTCGAGGAATATTGTCATCAGACACCGGAGGCCAGAGAAAGTACCTATTTCAATTATAACTTATACCAT
GTCCCTTTCCCTGGCATTAGTAAAGAAGCACATAAAGTATTATTAGAACTACAAGGACTCTTTGGAAAAGAGGCGCTAGC
CACTTCATTTAAGCAAAAAGTCATGCCATCCCTGTATTACGCGCAAAAAATAGGCGCGACCTATTGTGGCAGCATTTTCC
TTTCCCTATTTTCACTTATGGTGAAAGCACCAGAGATTAAATCAGGGGATCGAATAGGGGTCTACTCTTATGGTTCAGGA
TCCTGTGCCGAGTATTTCAGCATGCAGGTCGGGGACCAGGCAGTAGAGATTGCACAACAGCAAGGTGCAGCGTTGGACAT
ACTGCTTACCCAGCGGAAAATACTGGATGTGGCGGCATACGAGCAACTGGAAAATGCACGTAGCGCACAAATGCAAGGTA
AAGACTATCGTTTAGACGGGGACAACTTACAGGACTGGTATACACACCATTACAGCGGGAGTGGGCTCCTTATTCTTGAG
CGTATCGATAACCACTTACGGCGTTATCGCTATGCATGA

Upstream 100 bases:

>100_bases
CATTTTATTTCTTGTTGCTAAAGAAAGTAGTTATGTAAACGGTGCGGTTTTCAATGTGACCGGTGGCATTGACTGGGTTT
ATTAACTTTGGGATACCTTA

Downstream 100 bases:

>100_bases
ATATGTCACTGTACCTATCAAATTTAGCCCCGATTCATTAGCCGATTTCGAAAATCGCTTTATCTCTGCGCTAAATGACG
AGCGCTATAAAGTCATTGTA

Product: hydroxymethylglutaryl-coenzyme A synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNSIGIEKISIYPSSLALKLSELAQARGGDNKYIQESLMVIKRSLVPQWEDAVTLAVNSAKDMLNDQDRSDIGLFIIATE
SGLDQEKAISTWAHRYLGLPATCRVFELKSACHAGAAALKMAESWISSGLNRGKKVLLINVDISNNMICHGKMEYSLGAG
AIALLISDNPVFAVIETGKSGIHTHEVTDVIRPLPWKEVAFDVEASLFSYMDGLENAFEEYCHQTPEARESTYFNYNLYH
VPFPGISKEAHKVLLELQGLFGKEALATSFKQKVMPSLYYAQKIGATYCGSIFLSLFSLMVKAPEIKSGDRIGVYSYGSG
SCAEYFSMQVGDQAVEIAQQQGAALDILLTQRKILDVAAYEQLENARSAQMQGKDYRLDGDNLQDWYTHHYSGSGLLILE
RIDNHLRRYRYA

Sequences:

>Translated_412_residues
MNSIGIEKISIYPSSLALKLSELAQARGGDNKYIQESLMVIKRSLVPQWEDAVTLAVNSAKDMLNDQDRSDIGLFIIATE
SGLDQEKAISTWAHRYLGLPATCRVFELKSACHAGAAALKMAESWISSGLNRGKKVLLINVDISNNMICHGKMEYSLGAG
AIALLISDNPVFAVIETGKSGIHTHEVTDVIRPLPWKEVAFDVEASLFSYMDGLENAFEEYCHQTPEARESTYFNYNLYH
VPFPGISKEAHKVLLELQGLFGKEALATSFKQKVMPSLYYAQKIGATYCGSIFLSLFSLMVKAPEIKSGDRIGVYSYGSG
SCAEYFSMQVGDQAVEIAQQQGAALDILLTQRKILDVAAYEQLENARSAQMQGKDYRLDGDNLQDWYTHHYSGSGLLILE
RIDNHLRRYRYA
>Mature_412_residues
MNSIGIEKISIYPSSLALKLSELAQARGGDNKYIQESLMVIKRSLVPQWEDAVTLAVNSAKDMLNDQDRSDIGLFIIATE
SGLDQEKAISTWAHRYLGLPATCRVFELKSACHAGAAALKMAESWISSGLNRGKKVLLINVDISNNMICHGKMEYSLGAG
AIALLISDNPVFAVIETGKSGIHTHEVTDVIRPLPWKEVAFDVEASLFSYMDGLENAFEEYCHQTPEARESTYFNYNLYH
VPFPGISKEAHKVLLELQGLFGKEALATSFKQKVMPSLYYAQKIGATYCGSIFLSLFSLMVKAPEIKSGDRIGVYSYGSG
SCAEYFSMQVGDQAVEIAQQQGAALDILLTQRKILDVAAYEQLENARSAQMQGKDYRLDGDNLQDWYTHHYSGSGLLILE
RIDNHLRRYRYA

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI148298764, Length=391, Percent_Identity=25.3196930946292, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI148298677, Length=391, Percent_Identity=25.3196930946292, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24654139, Length=380, Percent_Identity=23.4210526315789, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654137, Length=380, Percent_Identity=23.4210526315789, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654133, Length=380, Percent_Identity=23.4210526315789, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654135, Length=380, Percent_Identity=23.4210526315789, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17933694, Length=380, Percent_Identity=23.4210526315789, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 45771; Mature: 45771

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIGIEKISIYPSSLALKLSELAQARGGDNKYIQESLMVIKRSLVPQWEDAVTLAVNSA
CCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHH
KDMLNDQDRSDIGLFIIATESGLDQEKAISTWAHRYLGLPATCRVFELKSACHAGAAALK
HHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MAESWISSGLNRGKKVLLINVDISNNMICHGKMEYSLGAGAIALLISDNPVFAVIETGKS
HHHHHHHHHHCCCCEEEEEEEECCCCEEEECEEEEECCCCEEEEEEECCCEEEEEECCCC
GIHTHEVTDVIRPLPWKEVAFDVEASLFSYMDGLENAFEEYCHQTPEARESTYFNYNLYH
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEE
VPFPGISKEAHKVLLELQGLFGKEALATSFKQKVMPSLYYAQKIGATYCGSIFLSLFSLM
ECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VKAPEIKSGDRIGVYSYGSGSCAEYFSMQVGDQAVEIAQQQGAALDILLTQRKILDVAAY
HCCCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHH
EQLENARSAQMQGKDYRLDGDNLQDWYTHHYSGSGLLILERIDNHLRRYRYA
HHHHHHHHHHCCCCCEEECCCCCHHHHHHEECCCCEEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MNSIGIEKISIYPSSLALKLSELAQARGGDNKYIQESLMVIKRSLVPQWEDAVTLAVNSA
CCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHH
KDMLNDQDRSDIGLFIIATESGLDQEKAISTWAHRYLGLPATCRVFELKSACHAGAAALK
HHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MAESWISSGLNRGKKVLLINVDISNNMICHGKMEYSLGAGAIALLISDNPVFAVIETGKS
HHHHHHHHHHCCCCEEEEEEEECCCCEEEECEEEEECCCCEEEEEEECCCEEEEEECCCC
GIHTHEVTDVIRPLPWKEVAFDVEASLFSYMDGLENAFEEYCHQTPEARESTYFNYNLYH
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEE
VPFPGISKEAHKVLLELQGLFGKEALATSFKQKVMPSLYYAQKIGATYCGSIFLSLFSLM
ECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VKAPEIKSGDRIGVYSYGSGSCAEYFSMQVGDQAVEIAQQQGAALDILLTQRKILDVAAY
HCCCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHH
EQLENARSAQMQGKDYRLDGDNLQDWYTHHYSGSGLLILERIDNHLRRYRYA
HHHHHHHHHHCCCCCEEECCCCCHHHHHHEECCCCEEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]