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The map label for this gene is hisB [H]

Identifier: 170024753

GI number: 170024753

Start: 2770144

End: 2771211

Strand: Direct

Name: hisB [H]

Synonym: YPK_2528

Alternate gene names: 170024753

Gene position: 2770144-2771211 (Clockwise)

Preceding gene: 170024752

Following gene: 170024754

Centisome position: 59.07

GC content: 51.31

Gene sequence:

>1068_bases
ATGAGCCAGAAATTTCTTTTTATTGACCGCGACGGCACCATCATTGCCGAGCCACCAACTGATTATCAGGTTGACCGGTT
GGATAAACTGGCGCTGGAGCCTGATGTCATTCCCGCATTGCTGGCGTTGCAAAAAGCAGACTACAAACTGGTGATGATCA
CTAATCAGGATGGCCTCGGCACCAGCAGTTTCCCGCAGGAAACCTTCGATCCGCCACATAACCTGATGATGCAAATCCTG
ACGTCTCAGGGGATCAATTTTGAACAGATACTGATTTGCCCACATCTGCCAGCCGATAACTGCACCTGTCGCAAACCGAA
AACCGCGCTGGTAGAAAGCTATCTGGCAGACGGCGTGATGAACAGTGCCACTAGCTATGTCATCGGTGACCGTGAAACTG
ACCTACAACTGGCCGAGAACATGGGTATCAGCGGGTTACGTTATCAGCGTGATGGCTTGAACTGGACGCAAATTGCCAAA
CAACTGACCCAGCGCGACCGCCACGCCTATGTTAATCGCGTGACCAAAGAAACCGCCATTGACGTTAATGTTTGGCTGGA
TCGCGAAGGGGGAAGCAAAATTAAAACCGGCGTGGGCTTCTTCGACCATATGCTGGATCAAATCGCCACCCACGGCGGTT
TTCGCATGGATATTCAGGTCAGCGGCGATCTGTATATCGATGATCACCACACAGTGGAAGATACCGCGCTGGCACTGGGC
GAAGCGATCAACATCGCACTGGGTGACAAACGGGGTATTGGCCGCTTTGGTTTTGTATTGCCGATGGATGAGTGCCTGGC
ACGCTGTGCCTTGGATATTTCTGGTCGCCCGCATTTGGAATACAAAGCTGAATTTAACTACCAGCGTGTCGGCGATCTAA
GCACCGAGATGGTCGAGCACTTCTTCCGCTCCCTTTCGTATGCCATGGCCTGTACCTTGCACCTGAAAACCAAAGGTCGC
AACGATCATCACCGAGTAGAAAGCCTGTTTAAAGTATTTGGTCGTACCTTGCGTCAAGCCATTCGCGTTGAAGGCAATAC
CCTGCCAAGTTCAAAAGGAGTGCTGTAA

Upstream 100 bases:

>100_bases
TTGCCGCCCTTGCCCCCCTGGCCGGCATTGATAACTCAAATAACATTGATAACCAGAGTAAAACCCATTCTCAGACCTCC
AGCATCCGTAAGGGAACGAT

Downstream 100 bases:

>100_bases
TGGATGTGGTGATCCTGGATACCGGTTGTGCCAATCTCTCCTCTGTCACCTATGCGGTACAGCGTTTGGGTTATACACCG
GTTATCAGCCGCGACCCGGC

Product: imidazole glycerol-phosphate dehydratase/histidinol phosphatase

Products: NA

Alternate protein names: Histidinol-phosphatase; Imidazoleglycerol-phosphate dehydratase; IGPD [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSQKFLFIDRDGTIIAEPPTDYQVDRLDKLALEPDVIPALLALQKADYKLVMITNQDGLGTSSFPQETFDPPHNLMMQIL
TSQGINFEQILICPHLPADNCTCRKPKTALVESYLADGVMNSATSYVIGDRETDLQLAENMGISGLRYQRDGLNWTQIAK
QLTQRDRHAYVNRVTKETAIDVNVWLDREGGSKIKTGVGFFDHMLDQIATHGGFRMDIQVSGDLYIDDHHTVEDTALALG
EAINIALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFNYQRVGDLSTEMVEHFFRSLSYAMACTLHLKTKGR
NDHHRVESLFKVFGRTLRQAIRVEGNTLPSSKGVL

Sequences:

>Translated_355_residues
MSQKFLFIDRDGTIIAEPPTDYQVDRLDKLALEPDVIPALLALQKADYKLVMITNQDGLGTSSFPQETFDPPHNLMMQIL
TSQGINFEQILICPHLPADNCTCRKPKTALVESYLADGVMNSATSYVIGDRETDLQLAENMGISGLRYQRDGLNWTQIAK
QLTQRDRHAYVNRVTKETAIDVNVWLDREGGSKIKTGVGFFDHMLDQIATHGGFRMDIQVSGDLYIDDHHTVEDTALALG
EAINIALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFNYQRVGDLSTEMVEHFFRSLSYAMACTLHLKTKGR
NDHHRVESLFKVFGRTLRQAIRVEGNTLPSSKGVL
>Mature_354_residues
SQKFLFIDRDGTIIAEPPTDYQVDRLDKLALEPDVIPALLALQKADYKLVMITNQDGLGTSSFPQETFDPPHNLMMQILT
SQGINFEQILICPHLPADNCTCRKPKTALVESYLADGVMNSATSYVIGDRETDLQLAENMGISGLRYQRDGLNWTQIAKQ
LTQRDRHAYVNRVTKETAIDVNVWLDREGGSKIKTGVGFFDHMLDQIATHGGFRMDIQVSGDLYIDDHHTVEDTALALGE
AINIALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFNYQRVGDLSTEMVEHFFRSLSYAMACTLHLKTKGRN
DHHRVESLFKVFGRTLRQAIRVEGNTLPSSKGVL

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI87082027, Length=355, Percent_Identity=81.6901408450704, Blast_Score=616, Evalue=1e-178,
Organism=Escherichia coli, GI1786399, Length=150, Percent_Identity=30, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324776, Length=217, Percent_Identity=39.1705069124424, Blast_Score=156, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006549
- InterPro:   IPR020566
- InterPro:   IPR005954
- InterPro:   IPR006543
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR013954
- InterPro:   IPR020568 [H]

Pfam domain/function: PF00475 IGPD; PF08645 PNK3P [H]

EC number: =3.1.3.15; =4.2.1.19 [H]

Molecular weight: Translated: 39835; Mature: 39704

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00954 IGP_DEHYDRATASE_1 ; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKFLFIDRDGTIIAEPPTDYQVDRLDKLALEPDVIPALLALQKADYKLVMITNQDGLG
CCCCEEEEECCCEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCC
TSSFPQETFDPPHNLMMQILTSQGINFEQILICPHLPADNCTCRKPKTALVESYLADGVM
CCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
NSATSYVIGDRETDLQLAENMGISGLRYQRDGLNWTQIAKQLTQRDRHAYVNRVTKETAI
HCCCEEEECCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
DVNVWLDREGGSKIKTGVGFFDHMLDQIATHGGFRMDIQVSGDLYIDDHHTVEDTALALG
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCHHHHHHHHH
EAINIALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFNYQRVGDLSTEMVEH
HHEEEEECCCCCCCCCEEECCHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCHHHHHHHH
FFRSLSYAMACTLHLKTKGRNDHHRVESLFKVFGRTLRQAIRVEGNTLPSSKGVL
HHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SQKFLFIDRDGTIIAEPPTDYQVDRLDKLALEPDVIPALLALQKADYKLVMITNQDGLG
CCCEEEEECCCEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCC
TSSFPQETFDPPHNLMMQILTSQGINFEQILICPHLPADNCTCRKPKTALVESYLADGVM
CCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
NSATSYVIGDRETDLQLAENMGISGLRYQRDGLNWTQIAKQLTQRDRHAYVNRVTKETAI
HCCCEEEECCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
DVNVWLDREGGSKIKTGVGFFDHMLDQIATHGGFRMDIQVSGDLYIDDHHTVEDTALALG
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCHHHHHHHHH
EAINIALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFNYQRVGDLSTEMVEH
HHEEEEECCCCCCCCCEEECCHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCHHHHHHHH
FFRSLSYAMACTLHLKTKGRNDHHRVESLFKVFGRTLRQAIRVEGNTLPSSKGVL
HHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]