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The map label for this gene is eda [H]

Identifier: 170024366

GI number: 170024366

Start: 2363192

End: 2363833

Strand: Reverse

Name: eda [H]

Synonym: YPK_2132

Alternate gene names: 170024366

Gene position: 2363833-2363192 (Counterclockwise)

Preceding gene: 170024367

Following gene: 170024365

Centisome position: 50.41

GC content: 51.87

Gene sequence:

>642_bases
ATGAAAAGCTGGAAAACGAGCGCAGAACAGATTATGACCGCAGGTCCTGTTGTCCCGGTGATCGTGATCAATAAGCTCGA
GCAAGCAGTACCGTTGGCAAAAGCTCTGGTGGCAGGCGGTGTTCGCGTACTGGAAGTGACGTTGCGTACCCCTTGTGCTG
TGGAGGCCATCCGCGCGATTGCCAAAGAAGTTCCTGAGGCAATTATTGGTGCTGGAACCGTGCTTAACCCACAACAACTG
GCTGAAGTGGTTGAAGCTGGCGCTCAGTTTGCCATTAGCCCTGGTTTGACTGATGAACTGCTTAAAGCGGCCTGCGAAGG
GTCTATCCCATTGATTCCAGGTATCAGCACCGTTTCAGAACTGATGCTGGGGATGAGCTATGGTTTGCGTGAGTTTAAAT
TCTTCCCGGCTGAAGCTAACGGTGGTGTCAAAGCGTTGCAAGCCATTGGCGGCCCATTCTCGCAAGTCCGTTTCTGCCCA
ACTGGCGGTATCACACCAAATAACTACCGTGATTATTTAGCACTGAAGAGTGTATTGTGCATTGGTGGCTCATGGTTGGT
TCCTGCTGACGCGTTGGAGAGTGGTGACTACGCGCGCATTACTGAATTAGCGAAGCAAGCGGTTGAAGGTGCGGCAGCAT
AA

Upstream 100 bases:

>100_bases
TCGCAGTTGTTCCATCGGGAGCGGCGATCAGAGTGCCATATTTCAACTAACACCTTCGGGTGAGTACAATGCCAGTCGGC
ATAAGTGGAGATAACAACTA

Downstream 100 bases:

>100_bases
GCCGCTCTAACTCCCTGACATAACGGGAGAATCCGTATATTTTCACCAAAGACAGAGCCTCTGCATTGCAGGGGTTTTGT
TTTTTCAGAATCGCTAACTT

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKSWKTSAEQIMTAGPVVPVIVINKLEQAVPLAKALVAGGVRVLEVTLRTPCAVEAIRAIAKEVPEAIIGAGTVLNPQQL
AEVVEAGAQFAISPGLTDELLKAACEGSIPLIPGISTVSELMLGMSYGLREFKFFPAEANGGVKALQAIGGPFSQVRFCP
TGGITPNNYRDYLALKSVLCIGGSWLVPADALESGDYARITELAKQAVEGAAA

Sequences:

>Translated_213_residues
MKSWKTSAEQIMTAGPVVPVIVINKLEQAVPLAKALVAGGVRVLEVTLRTPCAVEAIRAIAKEVPEAIIGAGTVLNPQQL
AEVVEAGAQFAISPGLTDELLKAACEGSIPLIPGISTVSELMLGMSYGLREFKFFPAEANGGVKALQAIGGPFSQVRFCP
TGGITPNNYRDYLALKSVLCIGGSWLVPADALESGDYARITELAKQAVEGAAA
>Mature_213_residues
MKSWKTSAEQIMTAGPVVPVIVINKLEQAVPLAKALVAGGVRVLEVTLRTPCAVEAIRAIAKEVPEAIIGAGTVLNPQQL
AEVVEAGAQFAISPGLTDELLKAACEGSIPLIPGISTVSELMLGMSYGLREFKFFPAEANGGVKALQAIGGPFSQVRFCP
TGGITPNNYRDYLALKSVLCIGGSWLVPADALESGDYARITELAKQAVEGAAA

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=211, Percent_Identity=87.6777251184834, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI48994954, Length=167, Percent_Identity=28.1437125748503, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22147; Mature: 22147

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSWKTSAEQIMTAGPVVPVIVINKLEQAVPLAKALVAGGVRVLEVTLRTPCAVEAIRAI
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
AKEVPEAIIGAGTVLNPQQLAEVVEAGAQFAISPGLTDELLKAACEGSIPLIPGISTVSE
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCCCCHHHHHH
LMLGMSYGLREFKFFPAEANGGVKALQAIGGPFSQVRFCPTGGITPNNYRDYLALKSVLC
HHHHHHHCHHHHEECCCCCCCHHHHHHHHCCCHHHEEECCCCCCCCCCHHHHHHHHHHHH
IGGSWLVPADALESGDYARITELAKQAVEGAAA
HCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSWKTSAEQIMTAGPVVPVIVINKLEQAVPLAKALVAGGVRVLEVTLRTPCAVEAIRAI
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
AKEVPEAIIGAGTVLNPQQLAEVVEAGAQFAISPGLTDELLKAACEGSIPLIPGISTVSE
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCCCCHHHHHH
LMLGMSYGLREFKFFPAEANGGVKALQAIGGPFSQVRFCPTGGITPNNYRDYLALKSVLC
HHHHHHHCHHHHEECCCCCCCHHHHHHHHCCCHHHEEECCCCCCCCCCHHHHHHHHHHHH
IGGSWLVPADALESGDYARITELAKQAVEGAAA
HCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]