Click here to switch to the map view.

The map label for this gene is apxIIID [H]

Identifier: 170024238

GI number: 170024238

Start: 2229281

End: 2230651

Strand: Reverse

Name: apxIIID [H]

Synonym: YPK_2003

Alternate gene names: 170024238

Gene position: 2230651-2229281 (Counterclockwise)

Preceding gene: 170024239

Following gene: 170024237

Centisome position: 47.57

GC content: 48.43

Gene sequence:

>1371_bases
ATGTCTGCGTTTATTCAGCACATAAAAAACCGCTGGACGCGTTGTTTTACCCCCGCGCGTACCCGTGATGAATATGATTT
TTTGCCTGCTTATTTGGAAATTGTCGAGCGCCCGATTGCCCCATTAGCCCGTCGTACAGCGTGGTTACTCATACTGACAT
TATTGCTGGTATTGATATGGGCGATTATTGGAAAACTTGATATCCATGCCTCAGCCAGCGGCAAAGTGATTGTTGCGGAA
CATTCCAAAATTATCCAACCCGCAGAACCTGGAGTGGTGACGGAAATTAATGTCCGAGATGGGGATACCGTTGATGCCGG
GCAGGTGCTTATTGCATTAAACCCTATTGGCATTGATGCCGAAGTACGCAATATCAATCAGCAACTACAGTATCGTCAGT
TAGAAGCCGCTCGGTTAGTGGCTCTCCTGGCTGATGAACCGCTAAAAAATTTCAAGCCGCCTTATAACAGCCCTGAATCT
CTGGTGGTGGCATCCAGAGCACTGTTAACAAAAGAATATGCCGAAGTGACAGCTGAATTGTCACGTCAGGATAGCGAACT
GGCGGTGAATCAGGCGCATCTTCAGGCCGGAATTGAGCACAACTCTCATCAAAAAGCATTGCTGAAAAATATTCATCGGC
GGCTACAAGCGTCGCGTACGCTGGCTAAATCTAAGTCAATTGCCGAGGTCGAGCTTCTGGTACAGGAGCGGGAATGGTTA
ACGGCAACGGCTGAAGCCAACCGTTTGCAGAGTGAACAAGATATTTTACGGGCCAAAGCACACAGTTTGGCGCAAACCCG
TATGCACTATCTGGCGAAAATAGACCGTGATAATCGGGAGCGATTGAATAAAACCCACGAAATTATTCATCAGCTTCAGC
AAGAGCAAATCAAAATGATGGATAAACAACGCCAGCAAACCCTAAGAGCTCCGGTGGCTGGCGTTATCCAGCAATTAGCG
GTTCATACCTTAGGGGGGGTGGTTACCACGGCGCAACCGTTAATGGTTCTGGTTCCGGAGGATTATCAGTTGGAACTTGA
TGTCATGATCCTCAATAAAGATGTGGGGTTCGTCCTGCCAGGCCAAGCGGTAGAAGTCAAAGTTGACAGTTTCCCTTATA
CCCGCTTCGGTACGTTATCCGGGGAAGTAAAACATGTTTCCCGTGATGCAATGGAAGATCAACAACGGGGACTGGTATTT
CCAGCGCGTATCCGTTTACTCAGCGATACCCTGATGGTGGAGGGTAAACCGGTGCGGTTGTCTGCGGGGATGGCCATCAA
TGCGGAAATTAAAACAGGTCGCCGTCGGGTCATTGATTATCTGCTGAGTCCATTGCAGCAATATCAATCTGAAGCCATGA
GGGAGCGCTAA

Upstream 100 bases:

>100_bases
TTACGCTCGGCTTTGGCAACAACAAGTTCATTTTAACCAAAACAAGTCCACCTTAACTAAAACAAGCCCACTTTAACCAC
GGGGAAATAAAATAAATATT

Downstream 100 bases:

>100_bases
CATGACGGAGTCTTATTCAACTCTGGCCGCCGAGGGCACCAAGGTTATTCAGCCACTGGAGGCGTTGGCGTGTGCTGCTA
CCTGTTTTGATTTAACCATC

Product: HlyD family type I secretion membrane fusion protein

Products: NA

Alternate protein names: APX-IIID; Cytolysin IIID; CLY-IIID; Toxin RTX-III secretion protein D [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSAFIQHIKNRWTRCFTPARTRDEYDFLPAYLEIVERPIAPLARRTAWLLILTLLLVLIWAIIGKLDIHASASGKVIVAE
HSKIIQPAEPGVVTEINVRDGDTVDAGQVLIALNPIGIDAEVRNINQQLQYRQLEAARLVALLADEPLKNFKPPYNSPES
LVVASRALLTKEYAEVTAELSRQDSELAVNQAHLQAGIEHNSHQKALLKNIHRRLQASRTLAKSKSIAEVELLVQEREWL
TATAEANRLQSEQDILRAKAHSLAQTRMHYLAKIDRDNRERLNKTHEIIHQLQQEQIKMMDKQRQQTLRAPVAGVIQQLA
VHTLGGVVTTAQPLMVLVPEDYQLELDVMILNKDVGFVLPGQAVEVKVDSFPYTRFGTLSGEVKHVSRDAMEDQQRGLVF
PARIRLLSDTLMVEGKPVRLSAGMAINAEIKTGRRRVIDYLLSPLQQYQSEAMRER

Sequences:

>Translated_456_residues
MSAFIQHIKNRWTRCFTPARTRDEYDFLPAYLEIVERPIAPLARRTAWLLILTLLLVLIWAIIGKLDIHASASGKVIVAE
HSKIIQPAEPGVVTEINVRDGDTVDAGQVLIALNPIGIDAEVRNINQQLQYRQLEAARLVALLADEPLKNFKPPYNSPES
LVVASRALLTKEYAEVTAELSRQDSELAVNQAHLQAGIEHNSHQKALLKNIHRRLQASRTLAKSKSIAEVELLVQEREWL
TATAEANRLQSEQDILRAKAHSLAQTRMHYLAKIDRDNRERLNKTHEIIHQLQQEQIKMMDKQRQQTLRAPVAGVIQQLA
VHTLGGVVTTAQPLMVLVPEDYQLELDVMILNKDVGFVLPGQAVEVKVDSFPYTRFGTLSGEVKHVSRDAMEDQQRGLVF
PARIRLLSDTLMVEGKPVRLSAGMAINAEIKTGRRRVIDYLLSPLQQYQSEAMRER
>Mature_455_residues
SAFIQHIKNRWTRCFTPARTRDEYDFLPAYLEIVERPIAPLARRTAWLLILTLLLVLIWAIIGKLDIHASASGKVIVAEH
SKIIQPAEPGVVTEINVRDGDTVDAGQVLIALNPIGIDAEVRNINQQLQYRQLEAARLVALLADEPLKNFKPPYNSPESL
VVASRALLTKEYAEVTAELSRQDSELAVNQAHLQAGIEHNSHQKALLKNIHRRLQASRTLAKSKSIAEVELLVQEREWLT
ATAEANRLQSEQDILRAKAHSLAQTRMHYLAKIDRDNRERLNKTHEIIHQLQQEQIKMMDKQRQQTLRAPVAGVIQQLAV
HTLGGVVTTAQPLMVLVPEDYQLELDVMILNKDVGFVLPGQAVEVKVDSFPYTRFGTLSGEVKHVSRDAMEDQQRGLVFP
ARIRLLSDTLMVEGKPVRLSAGMAINAEIKTGRRRVIDYLLSPLQQYQSEAMRER

Specific function: Involved in the transport of the toxin RTX-III [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003997
- InterPro:   IPR006143
- InterPro:   IPR006144
- InterPro:   IPR010129 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 51469; Mature: 51338

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00142 ZINC_PROTEASE ; PS00543 HLYD_FAMILY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFIQHIKNRWTRCFTPARTRDEYDFLPAYLEIVERPIAPLARRTAWLLILTLLLVLIW
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AIIGKLDIHASASGKVIVAEHSKIIQPAEPGVVTEINVRDGDTVDAGQVLIALNPIGIDA
HHHCCCEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCH
EVRNINQQLQYRQLEAARLVALLADEPLKNFKPPYNSPESLVVASRALLTKEYAEVTAEL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SRQDSELAVNQAHLQAGIEHNSHQKALLKNIHRRLQASRTLAKSKSIAEVELLVQEREWL
HCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TATAEANRLQSEQDILRAKAHSLAQTRMHYLAKIDRDNRERLNKTHEIIHQLQQEQIKMM
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
DKQRQQTLRAPVAGVIQQLAVHTLGGVVTTAQPLMVLVPEDYQLELDVMILNKDVGFVLP
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEECCCCEEEEEEEEEECCCCEEEC
GQAVEVKVDSFPYTRFGTLSGEVKHVSRDAMEDQQRGLVFPARIRLLSDTLMVEGKPVRL
CCEEEEEECCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCEEE
SAGMAINAEIKTGRRRVIDYLLSPLQQYQSEAMRER
ECCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAFIQHIKNRWTRCFTPARTRDEYDFLPAYLEIVERPIAPLARRTAWLLILTLLLVLIW
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AIIGKLDIHASASGKVIVAEHSKIIQPAEPGVVTEINVRDGDTVDAGQVLIALNPIGIDA
HHHCCCEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCH
EVRNINQQLQYRQLEAARLVALLADEPLKNFKPPYNSPESLVVASRALLTKEYAEVTAEL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SRQDSELAVNQAHLQAGIEHNSHQKALLKNIHRRLQASRTLAKSKSIAEVELLVQEREWL
HCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TATAEANRLQSEQDILRAKAHSLAQTRMHYLAKIDRDNRERLNKTHEIIHQLQQEQIKMM
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
DKQRQQTLRAPVAGVIQQLAVHTLGGVVTTAQPLMVLVPEDYQLELDVMILNKDVGFVLP
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEECCCCEEEEEEEEEECCCCEEEC
GQAVEVKVDSFPYTRFGTLSGEVKHVSRDAMEDQQRGLVFPARIRLLSDTLMVEGKPVRL
CCEEEEEECCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCEEE
SAGMAINAEIKTGRRRVIDYLLSPLQQYQSEAMRER
ECCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8494611; 7927703 [H]