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The map label for this gene is ilvB [H]

Identifier: 170024187

GI number: 170024187

Start: 2165246

End: 2166913

Strand: Reverse

Name: ilvB [H]

Synonym: YPK_1950

Alternate gene names: 170024187

Gene position: 2166913-2165246 (Counterclockwise)

Preceding gene: 170024189

Following gene: 170024186

Centisome position: 46.21

GC content: 50.24

Gene sequence:

>1668_bases
ATGCGTTATACAGGCGCCCAATTGATAGTTCGGTTACTGGAACAACAAGGCATTACTCTTGTAAGCGGTATTCCCGGCGG
TGCAGCCTTACCGCTGTATGATGCCCTAGGGCAAAGCCGTATTATTCGTCATGTATTGGCACGACATGAGCAAGGTGCGG
GTTTTATGGCTCAGGGAATGGCCCGAGCAACCGGTGAAACGGCAGTTTGTTTAGCCTCCAGTGGGCCAGGTGCAACCAAT
TTAGTGACCGCGATTGCCGATGCCAAACTTGATTCAATCCCATTAGTTTGTATCACTGGCCAAGTCCCATCTTCTATGAT
CGGTACCGATGCGTTTCAAGAAGTTGATACTTACGGCATCTCAATACCCATCACTAAACACAACTACCTCGTCCGCGATA
TCAGCGAGTTGGCGCAGGTTATTCCACAAGCGTTCCGGATTGCTCAATCAGGGCGGCCCGGCCCGGTATGGATCGATATT
CCCAAGGATGTACAAACGGCGGAAATTGACTTGGCTACCCTGCCACCACCGGGAATTGCCGATACTCCTGGCCCAGTAGA
CCTCTCTGCTATCGCGCAAGTCGCCAAGATGATTAACCAATCTGTACGGCCAGTGCTCTATTTAGGTGGGGGAATTGTCA
GTTCAGGGGCGCATCAGCAAGCAATACAATTGGCGGAACTGGCAAGTCTGCCGACGACAATGACGTTAATGGCATTAGGC
ACAATGCCCGTTGACCACCCATTATCACTGGGCATGTTGGGTATGCATGCGGCTCGCTCGACCAACCTGATTATGCAACA
AGCGGATTTATTGATTGTTCTCGGCGCACGGTTTGATGATCGGGCCATCGGAAAGGCAGAACAATTCTGCCCTCATGCCA
ATATTATTCATATTGATATTGACCCGGCAGAGTTGGGGAAAATCCGTTGCCCACATTTGGCAATGAATGCCGATATTGCG
CAGGTATTGACACATTTATTGCCAATGATCGACGCTCAGCTACGCAGCGATTGGCGTTCAACCGTTGCCGATATGCAGCG
TGAATTTCCATTCAACCAACCCAATAGCGAAAATCCACTGTGCCATTATGGGTTGATTCGTGCTGCTGCCGAAGCACTGG
ATGACGAGACCATTATTACCACCGATGTCGGGCAGCATCAGATGTGGGTGGCACAGGCTTATCCACTGCATCGCCCACGC
CAATGGTTAACCTCCGGCGGGCTAGGAACCATGGGCTTTGGCTTACCCGCCGCGATTGGTGCCGCTCTGGCAAAACCAGA
AAGTAAAGTGGTCTGCTTCTCTGGTGACGGAAGCCTAATGATGAATATTCAGGAGATGGCGACCGCGGCTGAAGAGCAAC
TGAATATAAAAATTATTTTGATGAATAACCAATCGCTGGGATTAGTCCATCAACAACAGGACCTTTTCTTCGGCAAACGT
ATTTTTGCTGCTGATTACGCTTATCGCACTAACTTCATTCATATCGCCGAGGGATTCGGTTTCTCTACCTGCGACCTTAA
TACTGCCAGCGATCCTTATACTGCACTGCATGAAGCCTTAAATCGCCCCGGTCCGGTGCTTATCCACGCACTGATCAATG
TGGATGAGAAAGTTTACCCAATGGTGCCACCCGGCGCGGCAAATATCGATATGATTGGAGGGGAATAG

Upstream 100 bases:

>100_bases
AATCCAGAAAGATTCCCAAACCCCGCCGGCGCCAGCCGAGCGGGGTTTCTTTTTATCGCCCCTCTCAGTTACCGCACCGG
CCCTGATGAAGGATATAACT

Downstream 100 bases:

>100_bases
TATGATAAATCAACAAAAACCCACTCAGCAAGAATCTGCTCAACATGAATCCGCTCAACCAATACCCACTCGGGTCACTC
TGCTCCTGACGGTGCGTAAC

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MRYTGAQLIVRLLEQQGITLVSGIPGGAALPLYDALGQSRIIRHVLARHEQGAGFMAQGMARATGETAVCLASSGPGATN
LVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGISIPITKHNYLVRDISELAQVIPQAFRIAQSGRPGPVWIDI
PKDVQTAEIDLATLPPPGIADTPGPVDLSAIAQVAKMINQSVRPVLYLGGGIVSSGAHQQAIQLAELASLPTTMTLMALG
TMPVDHPLSLGMLGMHAARSTNLIMQQADLLIVLGARFDDRAIGKAEQFCPHANIIHIDIDPAELGKIRCPHLAMNADIA
QVLTHLLPMIDAQLRSDWRSTVADMQREFPFNQPNSENPLCHYGLIRAAAEALDDETIITTDVGQHQMWVAQAYPLHRPR
QWLTSGGLGTMGFGLPAAIGAALAKPESKVVCFSGDGSLMMNIQEMATAAEEQLNIKIILMNNQSLGLVHQQQDLFFGKR
IFAADYAYRTNFIHIAEGFGFSTCDLNTASDPYTALHEALNRPGPVLIHALINVDEKVYPMVPPGAANIDMIGGE

Sequences:

>Translated_555_residues
MRYTGAQLIVRLLEQQGITLVSGIPGGAALPLYDALGQSRIIRHVLARHEQGAGFMAQGMARATGETAVCLASSGPGATN
LVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGISIPITKHNYLVRDISELAQVIPQAFRIAQSGRPGPVWIDI
PKDVQTAEIDLATLPPPGIADTPGPVDLSAIAQVAKMINQSVRPVLYLGGGIVSSGAHQQAIQLAELASLPTTMTLMALG
TMPVDHPLSLGMLGMHAARSTNLIMQQADLLIVLGARFDDRAIGKAEQFCPHANIIHIDIDPAELGKIRCPHLAMNADIA
QVLTHLLPMIDAQLRSDWRSTVADMQREFPFNQPNSENPLCHYGLIRAAAEALDDETIITTDVGQHQMWVAQAYPLHRPR
QWLTSGGLGTMGFGLPAAIGAALAKPESKVVCFSGDGSLMMNIQEMATAAEEQLNIKIILMNNQSLGLVHQQQDLFFGKR
IFAADYAYRTNFIHIAEGFGFSTCDLNTASDPYTALHEALNRPGPVLIHALINVDEKVYPMVPPGAANIDMIGGE
>Mature_555_residues
MRYTGAQLIVRLLEQQGITLVSGIPGGAALPLYDALGQSRIIRHVLARHEQGAGFMAQGMARATGETAVCLASSGPGATN
LVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGISIPITKHNYLVRDISELAQVIPQAFRIAQSGRPGPVWIDI
PKDVQTAEIDLATLPPPGIADTPGPVDLSAIAQVAKMINQSVRPVLYLGGGIVSSGAHQQAIQLAELASLPTTMTLMALG
TMPVDHPLSLGMLGMHAARSTNLIMQQADLLIVLGARFDDRAIGKAEQFCPHANIIHIDIDPAELGKIRCPHLAMNADIA
QVLTHLLPMIDAQLRSDWRSTVADMQREFPFNQPNSENPLCHYGLIRAAAEALDDETIITTDVGQHQMWVAQAYPLHRPR
QWLTSGGLGTMGFGLPAAIGAALAKPESKVVCFSGDGSLMMNIQEMATAAEEQLNIKIILMNNQSLGLVHQQQDLFFGKR
IFAADYAYRTNFIHIAEGFGFSTCDLNTASDPYTALHEALNRPGPVLIHALINVDEKVYPMVPPGAANIDMIGGE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=496, Percent_Identity=27.6209677419355, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI21361361, Length=581, Percent_Identity=24.7848537005164, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=69.5652173913043, Blast_Score=798, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=564, Percent_Identity=39.7163120567376, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=33.9416058394161, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=28.8566243194192, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=26.032315978456, Blast_Score=141, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=567, Percent_Identity=26.63139329806, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=567, Percent_Identity=26.63139329806, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=585, Percent_Identity=24.957264957265, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=39.2294220665499, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6320816, Length=559, Percent_Identity=26.1180679785331, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323163, Length=488, Percent_Identity=21.7213114754098, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922626, Length=492, Percent_Identity=29.0650406504065, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59464; Mature: 59464

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYTGAQLIVRLLEQQGITLVSGIPGGAALPLYDALGQSRIIRHVLARHEQGAGFMAQGM
CCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCHHHHHH
ARATGETAVCLASSGPGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGI
HHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCHHHHHHHHCCE
SIPITKHNYLVRDISELAQVIPQAFRIAQSGRPGPVWIDIPKDVQTAEIDLATLPPPGIA
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEECCCCCCC
DTPGPVDLSAIAQVAKMINQSVRPVLYLGGGIVSSGAHQQAIQLAELASLPTTMTLMALG
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHC
TMPVDHPLSLGMLGMHAARSTNLIMQQADLLIVLGARFDDRAIGKAEQFCPHANIIHIDI
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCHHHHCCCCCEEEEEC
DPAELGKIRCPHLAMNADIAQVLTHLLPMIDAQLRSDWRSTVADMQREFPFNQPNSENPL
CHHHHCCEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CHYGLIRAAAEALDDETIITTDVGQHQMWVAQAYPLHRPRQWLTSGGLGTMGFGLPAAIG
HHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHHHH
AALAKPESKVVCFSGDGSLMMNIQEMATAAEEQLNIKIILMNNQSLGLVHQQQDLFFGKR
HHHCCCCCCEEEECCCCCEEEEHHHHHHHHHHHCEEEEEEECCCCCCEEECHHHHHHHHH
IFAADYAYRTNFIHIAEGFGFSTCDLNTASDPYTALHEALNRPGPVLIHALINVDEKVYP
HHHHHHHHHCCEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEC
MVPPGAANIDMIGGE
CCCCCCCCEEECCCC
>Mature Secondary Structure
MRYTGAQLIVRLLEQQGITLVSGIPGGAALPLYDALGQSRIIRHVLARHEQGAGFMAQGM
CCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCHHHHHH
ARATGETAVCLASSGPGATNLVTAIADAKLDSIPLVCITGQVPSSMIGTDAFQEVDTYGI
HHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCHHHHHHHHCCE
SIPITKHNYLVRDISELAQVIPQAFRIAQSGRPGPVWIDIPKDVQTAEIDLATLPPPGIA
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEECCCCCCC
DTPGPVDLSAIAQVAKMINQSVRPVLYLGGGIVSSGAHQQAIQLAELASLPTTMTLMALG
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHC
TMPVDHPLSLGMLGMHAARSTNLIMQQADLLIVLGARFDDRAIGKAEQFCPHANIIHIDI
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCHHHHCCCCCEEEEEC
DPAELGKIRCPHLAMNADIAQVLTHLLPMIDAQLRSDWRSTVADMQREFPFNQPNSENPL
CHHHHCCEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CHYGLIRAAAEALDDETIITTDVGQHQMWVAQAYPLHRPRQWLTSGGLGTMGFGLPAAIG
HHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHHHH
AALAKPESKVVCFSGDGSLMMNIQEMATAAEEQLNIKIILMNNQSLGLVHQQQDLFFGKR
HHHCCCCCCEEEECCCCCEEEEHHHHHHHHHHHCEEEEEEECCCCCCEEECHHHHHHHHH
IFAADYAYRTNFIHIAEGFGFSTCDLNTASDPYTALHEALNRPGPVLIHALINVDEKVYP
HHHHHHHHHCCEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEC
MVPPGAANIDMIGGE
CCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]