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The map label for this gene is rstA [H]

Identifier: 170024174

GI number: 170024174

Start: 2149219

End: 2149965

Strand: Reverse

Name: rstA [H]

Synonym: YPK_1937

Alternate gene names: 170024174

Gene position: 2149965-2149219 (Counterclockwise)

Preceding gene: 170024176

Following gene: 170024173

Centisome position: 45.85

GC content: 48.06

Gene sequence:

>747_bases
ATGAGCAAAATTGTTTTTGTGGAAGACGACCCAGAGGTCGGAAAGCTGATAGCGGCTTACCTTGGCAAGCATGACATTGA
TGTATTTGTTGAGCCACGTGGTGATACCGCACAAGCCGTTATTGAACAGCAACAGCCCGATCTGGTGTTGCTCGATATCA
TGTTACCTGGGAAAGATGGCATGACCCTGTGCCGTGACCTACGGCCCCACTATGATGGCCCGATAGTTTTGCTGACATCG
CTAGACAGCGATATGAACCATATTCTCTCCCTAGAGATGGGGGCGAATGACTATATTCTAAAAACGACACCGCCAGCCGT
GCTGCTCGCCCGCTTACGGCTACATTTGCGCCAACATAATCAACGGTTACGCCAACAGACTCCCTTGCAAGCCAAAGAGA
CAATAAGCTCCCCACCCACGGGTTACAATGCGATTCATTTTGGGCTGCTATGTATTGACCCAGTTAACCGGCAAGTCAAT
TTGGATGATGAAGAAATTTCCTTATCAACTTCTGACTTTGATCTTTTATGGGAATTGGCGACACATGCTGGAATCATTAT
GGCTCGCGAGGCGTTATTGAAAAATTTACGCGGGATCAGCTATGACGGTATGGACCGAAGTATAGATGTCGCTATCTCTC
GCTTACGCCGCAAACTCTATGACAATGCACTGGACCCATTCCGGATCAAAACGGTGCGTAACAAAGGTTATTTGTTTGCG
CCCAATGCCTGGGAGTCCAAGCAGTAA

Upstream 100 bases:

>100_bases
TTGTTACGGCCTGTCTTGTTACTGCTAGTTTTGCTATTAACTAGTCTTGTCATTAACTGCACGCTATTCTGAGCCCAGCT
ATTTTTATAAGCAGGAAGAC

Downstream 100 bases:

>100_bases
TGAGAAAGCTATTTATTCAGTTTTTTTTGTTGTTATTTGTTTGCTTCATGGTGATGGCGATGCTGGTTGGCCTGGTTTAT
AAGGTCACCGCTGAGCGCGC

Product: DNA-binding transcriptional regulator RstA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSKIVFVEDDPEVGKLIAAYLGKHDIDVFVEPRGDTAQAVIEQQQPDLVLLDIMLPGKDGMTLCRDLRPHYDGPIVLLTS
LDSDMNHILSLEMGANDYILKTTPPAVLLARLRLHLRQHNQRLRQQTPLQAKETISSPPTGYNAIHFGLLCIDPVNRQVN
LDDEEISLSTSDFDLLWELATHAGIIMAREALLKNLRGISYDGMDRSIDVAISRLRRKLYDNALDPFRIKTVRNKGYLFA
PNAWESKQ

Sequences:

>Translated_248_residues
MSKIVFVEDDPEVGKLIAAYLGKHDIDVFVEPRGDTAQAVIEQQQPDLVLLDIMLPGKDGMTLCRDLRPHYDGPIVLLTS
LDSDMNHILSLEMGANDYILKTTPPAVLLARLRLHLRQHNQRLRQQTPLQAKETISSPPTGYNAIHFGLLCIDPVNRQVN
LDDEEISLSTSDFDLLWELATHAGIIMAREALLKNLRGISYDGMDRSIDVAISRLRRKLYDNALDPFRIKTVRNKGYLFA
PNAWESKQ
>Mature_247_residues
SKIVFVEDDPEVGKLIAAYLGKHDIDVFVEPRGDTAQAVIEQQQPDLVLLDIMLPGKDGMTLCRDLRPHYDGPIVLLTSL
DSDMNHILSLEMGANDYILKTTPPAVLLARLRLHLRQHNQRLRQQTPLQAKETISSPPTGYNAIHFGLLCIDPVNRQVNL
DDEEISLSTSDFDLLWELATHAGIIMAREALLKNLRGISYDGMDRSIDVAISRLRRKLYDNALDPFRIKTVRNKGYLFAP
NAWESKQ

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=245, Percent_Identity=73.0612244897959, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI1789809, Length=245, Percent_Identity=33.469387755102, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788394, Length=241, Percent_Identity=34.8547717842324, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI87082012, Length=242, Percent_Identity=33.8842975206612, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI2367329, Length=240, Percent_Identity=32.5, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786599, Length=241, Percent_Identity=29.4605809128631, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787229, Length=241, Percent_Identity=31.1203319502075, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786784, Length=238, Percent_Identity=31.0924369747899, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790863, Length=238, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786911, Length=239, Percent_Identity=26.3598326359833, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790860, Length=235, Percent_Identity=28.936170212766, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790552, Length=243, Percent_Identity=30.0411522633745, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1789402, Length=239, Percent_Identity=26.3598326359833, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1787375, Length=196, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28024; Mature: 27892

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIVFVEDDPEVGKLIAAYLGKHDIDVFVEPRGDTAQAVIEQQQPDLVLLDIMLPGKDG
CCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEEECCCCC
MTLCRDLRPHYDGPIVLLTSLDSDMNHILSLEMGANDYILKTTPPAVLLARLRLHLRQHN
HHHHHHCCCCCCCCEEEEEECCCCCHHEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHH
QRLRQQTPLQAKETISSPPTGYNAIHFGLLCIDPVNRQVNLDDEEISLSTSDFDLLWELA
HHHHHCCCCCHHHHCCCCCCCCCCEEEEEEEECCCCCEECCCHHHEEECCCCHHHHHHHH
THAGIIMAREALLKNLRGISYDGMDRSIDVAISRLRRKLYDNALDPFRIKTVRNKGYLFA
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEC
PNAWESKQ
CCCCCCCC
>Mature Secondary Structure 
SKIVFVEDDPEVGKLIAAYLGKHDIDVFVEPRGDTAQAVIEQQQPDLVLLDIMLPGKDG
CEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEEECCCCC
MTLCRDLRPHYDGPIVLLTSLDSDMNHILSLEMGANDYILKTTPPAVLLARLRLHLRQHN
HHHHHHCCCCCCCCEEEEEECCCCCHHEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHH
QRLRQQTPLQAKETISSPPTGYNAIHFGLLCIDPVNRQVNLDDEEISLSTSDFDLLWELA
HHHHHCCCCCHHHHCCCCCCCCCCEEEEEEEECCCCCEECCCHHHEEECCCCHHHHHHHH
THAGIIMAREALLKNLRGISYDGMDRSIDVAISRLRRKLYDNALDPFRIKTVRNKGYLFA
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEC
PNAWESKQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]