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The map label for this gene is ldhA [H]

Identifier: 170024157

GI number: 170024157

Start: 2125100

End: 2126092

Strand: Reverse

Name: ldhA [H]

Synonym: YPK_1918

Alternate gene names: 170024157

Gene position: 2126092-2125100 (Counterclockwise)

Preceding gene: 170024161

Following gene: 170024156

Centisome position: 45.34

GC content: 46.32

Gene sequence:

>993_bases
ATGAAATTAGCCGTTTACAGTACCAAGCAGTATGACCGTAAATACCTTGAGCTGGTCAACAAAGATTTTGGCTTTGAATT
GGAGTTTTTCGATTTTCTGCTTACCCCCAAAACCGCCAAAATGGCTGAGGGCTGTCAGGCGGTATGTCTCTTCGTCAATG
ATGATGGCTGCCGCGAAATATTAACTGAACTGGCGGCTATCGGCGTTAAAATTCTGGCATTGCGTTGTGCTGGCTTTAAT
AACGTGGATCTGGAAGCGGCCAAAGAGTTGGGTATACAAGTGGTCCGAGTGCCCGCATATTCACCTGAAGCTGTCGCGGA
ACATACCGTTGGCATGATGCTGAGCCTTAACCGACGTATTCACCGTGCTTATCAGCGAACCCGTGATGCCAATTTCTCGC
TGGAAGGCTTGATTGGTTTCAATATGCATAACCGCACTGCGGGGATTATCGGCACAGGTAAAATTGGTGTTGCCACCATG
CGTATCCTTAAAGGTTTTGGTATGCGCCTGCTGGCGTTTGACCCTTATCCAAGTGAGCAAGCTCTAGAGCTAGGTGCAGA
ATATGTTGATCTGAAGACCTTGTACGCCGAGTCTGATGTTATCTCACTGCATTGCCCGATGACGCCAGAAAACCATCATT
TGCTCAATAAGCAGTCATTTGATCAAATGAAAGATGGCGTGATGATCATTAATACCAGCCGTGGCGGTTTAATTGATTCA
ACGGCGGCGATCGATGCGTTAAAACAACAGAAAATAGGCTCCCTCGGCATGGACGTTTATGAAAATGAACGTGATTTATT
CTTTGAAGATAAATCCAATGACGTGATTCAGGATGATGTTTTCCGCCGTTTGTCCTCTTGCCATAATGTGTTATTTACTG
GGCATCAGGCGTTCCTAACCGAGGAAGCACTCACCAGTATCTCTGTCACCACCTTGCAGAATATTGCTCAGTTGGATAAA
GGCGAACCCTGCCCAAATATCATCACCCTCTGA

Upstream 100 bases:

>100_bases
TAATAAATTACACAGACTGCGATAAACTTATCATTGCTGAAAATTGTATGACTTATTGAAATATGTTAAATTTATCACAA
TATTTTGGCGGAGAAATACG

Downstream 100 bases:

>100_bases
TGCATTAACATCCAATAGCGGATAGCGAGTGAACATTCTCCCTATCCGTTATGATGAGTAGGAGATAATATGAGAAAGTT
CGTTCCAGTGGCTATTATCG

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKLAVYSTKQYDRKYLELVNKDFGFELEFFDFLLTPKTAKMAEGCQAVCLFVNDDGCREILTELAAIGVKILALRCAGFN
NVDLEAAKELGIQVVRVPAYSPEAVAEHTVGMMLSLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGIIGTGKIGVATM
RILKGFGMRLLAFDPYPSEQALELGAEYVDLKTLYAESDVISLHCPMTPENHHLLNKQSFDQMKDGVMIINTSRGGLIDS
TAAIDALKQQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSSCHNVLFTGHQAFLTEEALTSISVTTLQNIAQLDK
GEPCPNIITL

Sequences:

>Translated_330_residues
MKLAVYSTKQYDRKYLELVNKDFGFELEFFDFLLTPKTAKMAEGCQAVCLFVNDDGCREILTELAAIGVKILALRCAGFN
NVDLEAAKELGIQVVRVPAYSPEAVAEHTVGMMLSLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGIIGTGKIGVATM
RILKGFGMRLLAFDPYPSEQALELGAEYVDLKTLYAESDVISLHCPMTPENHHLLNKQSFDQMKDGVMIINTSRGGLIDS
TAAIDALKQQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSSCHNVLFTGHQAFLTEEALTSISVTTLQNIAQLDK
GEPCPNIITL
>Mature_330_residues
MKLAVYSTKQYDRKYLELVNKDFGFELEFFDFLLTPKTAKMAEGCQAVCLFVNDDGCREILTELAAIGVKILALRCAGFN
NVDLEAAKELGIQVVRVPAYSPEAVAEHTVGMMLSLNRRIHRAYQRTRDANFSLEGLIGFNMHNRTAGIIGTGKIGVATM
RILKGFGMRLLAFDPYPSEQALELGAEYVDLKTLYAESDVISLHCPMTPENHHLLNKQSFDQMKDGVMIINTSRGGLIDS
TAAIDALKQQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSSCHNVLFTGHQAFLTEEALTSISVTTLQNIAQLDK
GEPCPNIITL

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557497, Length=256, Percent_Identity=34.375, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI61743967, Length=256, Percent_Identity=34.375, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI23308577, Length=212, Percent_Identity=32.5471698113208, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI145580578, Length=256, Percent_Identity=32.8125, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4557499, Length=256, Percent_Identity=32.8125, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI145580575, Length=256, Percent_Identity=32.8125, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI6912396, Length=293, Percent_Identity=27.9863481228669, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1787645, Length=326, Percent_Identity=81.2883435582822, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI87082289, Length=191, Percent_Identity=34.0314136125654, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789279, Length=236, Percent_Identity=30.9322033898305, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788660, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=254, Percent_Identity=27.5590551181102, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25147481, Length=261, Percent_Identity=30.6513409961686, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=195, Percent_Identity=35.8974358974359, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=234, Percent_Identity=32.4786324786325, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=234, Percent_Identity=32.0512820512821, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=225, Percent_Identity=28, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325144, Length=126, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324980, Length=149, Percent_Identity=27.5167785234899, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24646446, Length=249, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646448, Length=249, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646452, Length=249, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646450, Length=249, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI62472511, Length=248, Percent_Identity=33.4677419354839, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19921140, Length=269, Percent_Identity=26.7657992565056, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28574286, Length=294, Percent_Identity=28.9115646258503, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45552429, Length=261, Percent_Identity=27.5862068965517, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585514, Length=261, Percent_Identity=27.5862068965517, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28574282, Length=261, Percent_Identity=27.5862068965517, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28574284, Length=261, Percent_Identity=27.5862068965517, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI45551003, Length=261, Percent_Identity=27.5862068965517, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28571528, Length=237, Percent_Identity=28.2700421940928, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=26.6331658291457, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36757; Mature: 36757

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVYSTKQYDRKYLELVNKDFGFELEFFDFLLTPKTAKMAEGCQAVCLFVNDDGCREI
CEEEEECCHHHHHHHHHHHCCCCCCEEEHHHHEECCCHHHHHCCCEEEEEEECCCCHHHH
LTELAAIGVKILALRCAGFNNVDLEAAKELGIQVVRVPAYSPEAVAEHTVGMMLSLNRRI
HHHHHHHHHHHHHEEECCCCCCCHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLIGFNMHNRTAGIIGTGKIGVATMRILKGFGMRLLAFDPYPSEQ
HHHHHHHCCCCCEEEEEEEECCCCCEECEEECCCHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGAEYVDLKTLYAESDVISLHCPMTPENHHLLNKQSFDQMKDGVMIINTSRGGLIDS
HHHHCCHHHHHHHEEECCCEEEEECCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCH
TAAIDALKQQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSSCHNVLFTGHQAFLT
HHHHHHHHHHHHCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHEECCCHHHHH
EEALTSISVTTLQNIAQLDKGEPCPNIITL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLAVYSTKQYDRKYLELVNKDFGFELEFFDFLLTPKTAKMAEGCQAVCLFVNDDGCREI
CEEEEECCHHHHHHHHHHHCCCCCCEEEHHHHEECCCHHHHHCCCEEEEEEECCCCHHHH
LTELAAIGVKILALRCAGFNNVDLEAAKELGIQVVRVPAYSPEAVAEHTVGMMLSLNRRI
HHHHHHHHHHHHHEEECCCCCCCHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLIGFNMHNRTAGIIGTGKIGVATMRILKGFGMRLLAFDPYPSEQ
HHHHHHHCCCCCEEEEEEEECCCCCEECEEECCCHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGAEYVDLKTLYAESDVISLHCPMTPENHHLLNKQSFDQMKDGVMIINTSRGGLIDS
HHHHCCHHHHHHHEEECCCEEEEECCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCH
TAAIDALKQQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSSCHNVLFTGHQAFLT
HHHHHHHHHHHHCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHEECCCHHHHH
EEALTSISVTTLQNIAQLDKGEPCPNIITL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]