Click here to switch to the map view.

The map label for this gene is pspF [H]

Identifier: 170024132

GI number: 170024132

Start: 2100613

End: 2101641

Strand: Reverse

Name: pspF [H]

Synonym: YPK_1893

Alternate gene names: 170024132

Gene position: 2101641-2100613 (Counterclockwise)

Preceding gene: 170024139

Following gene: 170024131

Centisome position: 44.82

GC content: 51.31

Gene sequence:

>1029_bases
ATGAATGAGCAACTGGATAATCTGCTCGGCGAAGCAAACGCGTTTGTTGACGTGCTGGAGCAAGTCTCGGGACTGGCAAA
ATTGAACAAACCCGTGTTAGTCATTGGGGAGCGTGGTACCGGTAAGGAATTGATTGCCCATCGCCTGCACTACTTATCAG
AACGTTGGCAAGGGCCATTTATCTCCCTTAACTGCGCCGCACTCAATGAGAACTTACTCGATTCAGAGTTATTCGGCCAT
GAAGCCGGGGCCTTTACTGGCGCGCAAAAACGTCATCTGGGCCGGTTTGAACGCGCGGATGGCGGCACTTTATTTTTGGA
TGAATTAGCGACAGCACCTATGCTGGTTCAGGAAAAATTATTACGCGTGATTGAGTACGGCCACTTGGAGCGTGTTGGCG
GCAGCCAGCCATTGCAGGTCGATGTCCGTTTAGTCTGCGCCACCAATGATAATCTGCCCGCGTTGGCGGCTGCCGGAAAA
TTCCGTGCCGACCTGCTAGACCGCCTCGCCTTTGATGTCGTGCAATTGCCCCCACTGCGTGAGCGGCAGCAAGATATCAT
GTTACTAGCCGAGCATTTCGCCATCCTAATGTGCCGTGAGCTGGGTCTTCCCCTCTTTTCTGGTTTTACTGCTACGGCGA
AAGAGCAATTACTCGAATACCGTTGGCCGGGGAATGTGCGTGAGCTAAAGAATGTGGTAGAGCGTTCGGTTTATCGCCAC
AGCGACAGCAGTCTGCCACTCAATAATATTATTATTAATCCTTTTGCTAGCAACCAGAAGGGTGAGATAGAGGGTGTGGA
TACGCCCAACGAGGGGGGAGCTGTGTTGCCCGCACTGCCCGTAGATTTGAAACATTGGCTGCACACCAGCGAACATCAGA
TGCTAACACGGGCATTAAAGCAGGCTCGGTTTAATCAGCGTAAGGCGGCGCATTTATTGGGGTTAACCTATCACCAACTA
CGAGGCTTATTAAAGAAACATACAATTTTGAGTGGGGAGTTGCCCCAAGTCAGTGATTCCGGTGAGTGA

Upstream 100 bases:

>100_bases
ATGATAATTAATAGCATGAAAATTAAAGAGAAAATAATTTTTATTCAAATGATATACAATATGAAGTGATTATTTTAACC
AACTATTGGCGAATTTCATC

Downstream 100 bases:

>100_bases
GTACAGCTAATTTTCATTGATACAAATAGCGCTGCAGCATCAAGGACGAAGGGTAAAAAAAAGCCAGCACCCGAGCTGGC
TTAAAATACTGGAAGCAATG

Product: phage shock protein operon transcriptional activator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNEQLDNLLGEANAFVDVLEQVSGLAKLNKPVLVIGERGTGKELIAHRLHYLSERWQGPFISLNCAALNENLLDSELFGH
EAGAFTGAQKRHLGRFERADGGTLFLDELATAPMLVQEKLLRVIEYGHLERVGGSQPLQVDVRLVCATNDNLPALAAAGK
FRADLLDRLAFDVVQLPPLRERQQDIMLLAEHFAILMCRELGLPLFSGFTATAKEQLLEYRWPGNVRELKNVVERSVYRH
SDSSLPLNNIIINPFASNQKGEIEGVDTPNEGGAVLPALPVDLKHWLHTSEHQMLTRALKQARFNQRKAAHLLGLTYHQL
RGLLKKHTILSGELPQVSDSGE

Sequences:

>Translated_342_residues
MNEQLDNLLGEANAFVDVLEQVSGLAKLNKPVLVIGERGTGKELIAHRLHYLSERWQGPFISLNCAALNENLLDSELFGH
EAGAFTGAQKRHLGRFERADGGTLFLDELATAPMLVQEKLLRVIEYGHLERVGGSQPLQVDVRLVCATNDNLPALAAAGK
FRADLLDRLAFDVVQLPPLRERQQDIMLLAEHFAILMCRELGLPLFSGFTATAKEQLLEYRWPGNVRELKNVVERSVYRH
SDSSLPLNNIIINPFASNQKGEIEGVDTPNEGGAVLPALPVDLKHWLHTSEHQMLTRALKQARFNQRKAAHLLGLTYHQL
RGLLKKHTILSGELPQVSDSGE
>Mature_342_residues
MNEQLDNLLGEANAFVDVLEQVSGLAKLNKPVLVIGERGTGKELIAHRLHYLSERWQGPFISLNCAALNENLLDSELFGH
EAGAFTGAQKRHLGRFERADGGTLFLDELATAPMLVQEKLLRVIEYGHLERVGGSQPLQVDVRLVCATNDNLPALAAAGK
FRADLLDRLAFDVVQLPPLRERQQDIMLLAEHFAILMCRELGLPLFSGFTATAKEQLLEYRWPGNVRELKNVVERSVYRH
SDSSLPLNNIIINPFASNQKGEIEGVDTPNEGGAVLPALPVDLKHWLHTSEHQMLTRALKQARFNQRKAAHLLGLTYHQL
RGLLKKHTILSGELPQVSDSGE

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=329, Percent_Identity=73.2522796352584, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1788550, Length=329, Percent_Identity=39.5136778115502, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082117, Length=242, Percent_Identity=47.5206611570248, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1790437, Length=324, Percent_Identity=41.0493827160494, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1790299, Length=337, Percent_Identity=41.2462908011869, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789087, Length=244, Percent_Identity=46.7213114754098, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789233, Length=339, Percent_Identity=39.2330383480826, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=38.0804953560372, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786524, Length=243, Percent_Identity=44.0329218106996, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1788905, Length=220, Percent_Identity=44.0909090909091, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787583, Length=220, Percent_Identity=42.7272727272727, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1789828, Length=235, Percent_Identity=34.468085106383, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI87081858, Length=210, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 38041; Mature: 38041

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQLDNLLGEANAFVDVLEQVSGLAKLNKPVLVIGERGTGKELIAHRLHYLSERWQGPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCE
ISLNCAALNENLLDSELFGHEAGAFTGAQKRHLGRFERADGGTLFLDELATAPMLVQEKL
EEEEHHHHCCHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCCEEEHHHHCCCHHHHHHHH
LRVIEYGHLERVGGSQPLQVDVRLVCATNDNLPALAAAGKFRADLLDRLAFDVVQLPPLR
HHHHHHCCHHHCCCCCCEEEEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCH
ERQQDIMLLAEHFAILMCRELGLPLFSGFTATAKEQLLEYRWPGNVRELKNVVERSVYRH
HHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
SDSSLPLNNIIINPFASNQKGEIEGVDTPNEGGAVLPALPVDLKHWLHTSEHQMLTRALK
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHCCCHHHHHHHHHH
QARFNQRKAAHLLGLTYHQLRGLLKKHTILSGELPQVSDSGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNEQLDNLLGEANAFVDVLEQVSGLAKLNKPVLVIGERGTGKELIAHRLHYLSERWQGPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCE
ISLNCAALNENLLDSELFGHEAGAFTGAQKRHLGRFERADGGTLFLDELATAPMLVQEKL
EEEEHHHHCCHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCCEEEHHHHCCCHHHHHHHH
LRVIEYGHLERVGGSQPLQVDVRLVCATNDNLPALAAAGKFRADLLDRLAFDVVQLPPLR
HHHHHHCCHHHCCCCCCEEEEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCH
ERQQDIMLLAEHFAILMCRELGLPLFSGFTATAKEQLLEYRWPGNVRELKNVVERSVYRH
HHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
SDSSLPLNNIIINPFASNQKGEIEGVDTPNEGGAVLPALPVDLKHWLHTSEHQMLTRALK
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHCCCHHHHHHHHHH
QARFNQRKAAHLLGLTYHQLRGLLKKHTILSGELPQVSDSGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]