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The map label for this gene is nemR [H]

Identifier: 170024111

GI number: 170024111

Start: 2077686

End: 2078285

Strand: Reverse

Name: nemR [H]

Synonym: YPK_1872

Alternate gene names: 170024111

Gene position: 2078285-2077686 (Counterclockwise)

Preceding gene: 170024112

Following gene: 170024110

Centisome position: 44.32

GC content: 50.5

Gene sequence:

>600_bases
ATGAACAAAATACAGCATACACATGTAGACACCCGAGAACATCTACTCGCCACAGGTGAAAGGCTCAGTTTACGCCTCGG
TTTTAATGGTATGGGGTTGAGCCTGCTGCTGACTACCGCGGGTATCCCAAAAGGCTCGTTTTATCACTATTTTCGTTCAA
AAGAAGTGTTTGGCGAGGCCATGCTCCAACGCTATTTTGACCGTTATGATGCGGAGATGGAAAGCCTGTTAGCCGGTTCA
CAGGGTGATAAACGCCATCATCTATTGCACTATTTTGCTCAATCTATTGCTAACTATTGTGGGAGTGAATGCCACAACGC
GTGTCTGGCGGTTAAACTCTCCGCTGAGGTGAGTGATTTATCCGAACCTATGCGCCATGCGTTGGATATCGGCACTGCTC
GAGTGATTGGCCGCTTGCAGGATGCCATTGAAGCCGGAATTGACGAAGGCTCTTTGCGAACGGTGCTCTCTCCGGCGGCA
ACCGCAGAAACGCTCTACGCCTTGTGGCTAGGCGCGTCGCTGCGGGCAAAAGTGAAACACTCTGTCGCCCCACTGACTTG
TGCACTGGGAAGCATTGAACTCATATTACAACCAGCCTAA

Upstream 100 bases:

>100_bases
GCCCGTAGCGGAGTGAATAGGGAGACCATAGCCTTGATGCCAGCGCCTTGGTTGGCATTTTTTTCTTGACCTAGACCAAT
CGGTCTATTAGGCTCTTCGT

Downstream 100 bases:

>100_bases
TAATCTACCGATCCTGACACTGACTTTTTATGTTTTTCAGGATTTACCCAGCAACTAGTTGACTGGTCTATTAATACAGG
ACGAACCATGAAAACTGCTA

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MNKIQHTHVDTREHLLATGERLSLRLGFNGMGLSLLLTTAGIPKGSFYHYFRSKEVFGEAMLQRYFDRYDAEMESLLAGS
QGDKRHHLLHYFAQSIANYCGSECHNACLAVKLSAEVSDLSEPMRHALDIGTARVIGRLQDAIEAGIDEGSLRTVLSPAA
TAETLYALWLGASLRAKVKHSVAPLTCALGSIELILQPA

Sequences:

>Translated_199_residues
MNKIQHTHVDTREHLLATGERLSLRLGFNGMGLSLLLTTAGIPKGSFYHYFRSKEVFGEAMLQRYFDRYDAEMESLLAGS
QGDKRHHLLHYFAQSIANYCGSECHNACLAVKLSAEVSDLSEPMRHALDIGTARVIGRLQDAIEAGIDEGSLRTVLSPAA
TAETLYALWLGASLRAKVKHSVAPLTCALGSIELILQPA
>Mature_199_residues
MNKIQHTHVDTREHLLATGERLSLRLGFNGMGLSLLLTTAGIPKGSFYHYFRSKEVFGEAMLQRYFDRYDAEMESLLAGS
QGDKRHHLLHYFAQSIANYCGSECHNACLAVKLSAEVSDLSEPMRHALDIGTARVIGRLQDAIEAGIDEGSLRTVLSPAA
TAETLYALWLGASLRAKVKHSVAPLTCALGSIELILQPA

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=200, Percent_Identity=51.5, Blast_Score=190, Evalue=6e-50,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 21796; Mature: 21796

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIQHTHVDTREHLLATGERLSLRLGFNGMGLSLLLTTAGIPKGSFYHYFRSKEVFGEA
CCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
MLQRYFDRYDAEMESLLAGSQGDKRHHLLHYFAQSIANYCGSECHNACLAVKLSAEVSDL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHH
SEPMRHALDIGTARVIGRLQDAIEAGIDEGSLRTVLSPAATAETLYALWLGASLRAKVKH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHCHHHHHHHHH
SVAPLTCALGSIELILQPA
HHHHHHHHHCCEEEEEECC
>Mature Secondary Structure
MNKIQHTHVDTREHLLATGERLSLRLGFNGMGLSLLLTTAGIPKGSFYHYFRSKEVFGEA
CCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
MLQRYFDRYDAEMESLLAGSQGDKRHHLLHYFAQSIANYCGSECHNACLAVKLSAEVSDL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHH
SEPMRHALDIGTARVIGRLQDAIEAGIDEGSLRTVLSPAATAETLYALWLGASLRAKVKH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHCHHHHHHHHH
SVAPLTCALGSIELILQPA
HHHHHHHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]