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The map label for this gene is nemA [H]

Identifier: 170024110

GI number: 170024110

Start: 2076501

End: 2077598

Strand: Reverse

Name: nemA [H]

Synonym: YPK_1871

Alternate gene names: 170024110

Gene position: 2077598-2076501 (Counterclockwise)

Preceding gene: 170024111

Following gene: 170024108

Centisome position: 44.3

GC content: 50.82

Gene sequence:

>1098_bases
ATGAAAACTGCTAAATTATTCTCCCCATTAAAGGTTGGCGCGCTCACGTTGCCAAACCGTGTCTTTATGGCCCCACTCAC
CCGCTTGCGTAGCATTGAGCCTGGTGATATCCCAACGCCGTTAATGGCCGAATATTATCGCCAACGGGCCAGTGCAGGCT
TGATTATCACGGAAGCCACTCAGATTTCTTTTCAGGCGAAAGGCTATGCTGGCGCTCCAGGGCTACATACTCAAGAACAG
TTGAACGCATGGAAAAAAGTGACTCAGGGTGTACATAATGAGGGTGGCCATATTGCCGTACAACTTTGGCACGTAGGCCG
TATTTCCCACAACAGTTTACAACCAGGCCAGCAAGCCCCTGTAGCACCATCGGCGATTGCCGCAGATACCCGTACCACCG
TGCGCGATGAAACAGGTGCTTGGGTACGTGTCCCTTGCTCTACCCCACGGGCGCTGGAGACAGAAGAGATTCCGGGCATC
ATTAATGATTTCCGTCAGGCAACCGCCAATGCCCGCGAAGCAGGATTTGACTACATTGAACTGCACGCAGCCCATGGTTA
TCTGTTGCATCAATTCATGTCCCCAGCTTCCAATCAGCGTACTGACCAATATGGCGGCAGTATTGAAAACCGTACCCGTC
TGACGTTGGAAGTGGTCGATGCCACCGCCGCTGAATGGGGGGCTGACCGAATTGGTATTCGTATTTCGCCATTAGGCCCA
TTTAATGGCCTCGATAATGGTGAAGACCAAGAAGAAGCCGCACTGTATCTGATTGATCAGTTGAATAAACGTAACATTGC
TTATTTGCATATTTCCGAGCCAGACTGGGCAGGCGGTAAGCCTTATTCTGATACGTTCCGTGCTGCTGTCCGTGCTCATT
TTAACGGCGTTATTGTTGGTGCTGGTGCTTACAGCGCAGAGAAAGCTGAATGCTTGATTCAAAAAGGGTTCATTGATGCG
GTGGCTTTTGGTCGCAGTTATATTTCTAACCCAGATCTGGTGACGCGCCTGCAACAACACGCGCCACTGAATGAGCCTGA
TAGCAATACTTTCTATGGCGGCGGTGCGAAGGGCTACACCGATTACCCAACGTTGTGA

Upstream 100 bases:

>100_bases
ACAACCAGCCTAATAATCTACCGATCCTGACACTGACTTTTTATGTTTTTCAGGATTTACCCAGCAACTAGTTGACTGGT
CTATTAATACAGGACGAACC

Downstream 100 bases:

>100_bases
TTTGTCTGTAATTTAAATATTCAATAACCGCCAGTTAGGCTAGTCGTCTAACTGGCTCAGACGACTAACAAACCCCGATG
ACGCGATCATGAACGGTGAG

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIITEATQISFQAKGYAGAPGLHTQEQ
LNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAPVAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGI
INDFRQATANAREAGFDYIELHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
FNGLDNGEDQEEAALYLIDQLNKRNIAYLHISEPDWAGGKPYSDTFRAAVRAHFNGVIVGAGAYSAEKAECLIQKGFIDA
VAFGRSYISNPDLVTRLQQHAPLNEPDSNTFYGGGAKGYTDYPTL

Sequences:

>Translated_365_residues
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIITEATQISFQAKGYAGAPGLHTQEQ
LNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAPVAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGI
INDFRQATANAREAGFDYIELHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
FNGLDNGEDQEEAALYLIDQLNKRNIAYLHISEPDWAGGKPYSDTFRAAVRAHFNGVIVGAGAYSAEKAECLIQKGFIDA
VAFGRSYISNPDLVTRLQQHAPLNEPDSNTFYGGGAKGYTDYPTL
>Mature_365_residues
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIITEATQISFQAKGYAGAPGLHTQEQ
LNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAPVAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGI
INDFRQATANAREAGFDYIELHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
FNGLDNGEDQEEAALYLIDQLNKRNIAYLHISEPDWAGGKPYSDTFRAAVRAHFNGVIVGAGAYSAEKAECLIQKGFIDA
VAFGRSYISNPDLVTRLQQHAPLNEPDSNTFYGGGAKGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=74.5205479452055, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1789463, Length=242, Percent_Identity=35.5371900826446, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17565138, Length=365, Percent_Identity=28.7671232876712, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17559802, Length=226, Percent_Identity=33.6283185840708, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=239, Percent_Identity=31.3807531380753, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=223, Percent_Identity=31.8385650224215, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=347, Percent_Identity=27.9538904899135, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=353, Percent_Identity=28.328611898017, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=217, Percent_Identity=27.6497695852535, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=381, Percent_Identity=38.3202099737533, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6321973, Length=384, Percent_Identity=36.4583333333333, Blast_Score=222, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39795; Mature: 39795

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIITEAT
CCCHHHCCCHHCCEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECE
QISFQAKGYAGAPGLHTQEQLNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAP
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCC
VAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGIINDFRQATANAREAGFDYIE
CCCHHHHCCCCHHEECCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHCCHHHCCCCEEE
LHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
EEHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEEEEEHHHHHCCCCCCCEEEECCCC
FNGLDNGEDQEEAALYLIDQLNKRNIAYLHISEPDWAGGKPYSDTFRAAVRAHFNGVIVG
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEE
AGAYSAEKAECLIQKGFIDAVAFGRSYISNPDLVTRLQQHAPLNEPDSNTFYGGGAKGYT
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIITEAT
CCCHHHCCCHHCCEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECE
QISFQAKGYAGAPGLHTQEQLNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAP
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCC
VAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGIINDFRQATANAREAGFDYIE
CCCHHHHCCCCHHEECCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHCCHHHCCCCEEE
LHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
EEHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEEEEEHHHHHCCCCCCCEEEECCCC
FNGLDNGEDQEEAALYLIDQLNKRNIAYLHISEPDWAGGKPYSDTFRAAVRAHFNGVIVG
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEE
AGAYSAEKAECLIQKGFIDAVAFGRSYISNPDLVTRLQQHAPLNEPDSNTFYGGGAKGYT
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]