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The map label for this gene is pykF [H]

Identifier: 170024094

GI number: 170024094

Start: 2060225

End: 2061637

Strand: Reverse

Name: pykF [H]

Synonym: YPK_1855

Alternate gene names: 170024094

Gene position: 2061637-2060225 (Counterclockwise)

Preceding gene: 170024095

Following gene: 170024093

Centisome position: 43.96

GC content: 48.55

Gene sequence:

>1413_bases
ATGAAAAAGACTAAAATTGTTTGTACTATCGGTCCAAAAACCGAATCCGAAGAAATGCTGACAAACCTGCTGAATGCTGG
CATGAACGTTATGCGTTTAAACTTCTCTCACGGTGACTATGCAGAGCACGGTCAACGTATCAAAAACCTCCGGGCTGTAA
TGGCAAAAACAGGCAAAAAAGCCGCGATTTTGCTGGATACTAAAGGCCCTGAAATCCGGACCATGAAACTGGAAGGCGGC
AAAGATGCTGCGCTGGTTGCAGGTCAGACCTTCACGTTCACGACTGATCAAAGCGTTGTCGGTAACGACAAAATTGTTGC
AGTCACCTACCCAGGCTTCGCCGCTGACCTGAAAATTGGTAACACTGTTCTGGTTGACGATGGCTTGATCGGTATGGAAG
TCATTGAAGTCACTGAACACACCGTTGTCTGTAAAGTCTTGAACAGTGGTGATTTGGGTGAAAACAAAGGCGTTAACCTG
CCAGGCGTGTCGATCCAACTGCCCGCATTAGCAGAAAAAGATAAAGGCGACCTGATCTTCGGTTGCGAACAAGGCGTTGA
TTTCGTTGCCGCCTCCTTTATCCGTAAACGTTCTGATGTACTGGAAATTCGTGAACACCTGAAAGCACACGGTGGCGAAC
ATATCCAGATCATCTCTAAAATCGAAAATCAGGAAGGTCTGAATAACTTCGACGAAATCCTGGAAGCCTCTGACGGTATC
ATGGTTGCCCGTGGTGACTTAGGTGTTGAGATCCCTGTCGAAGAAGTCATCTTCGCGCAGAAGATGATGATCGAGAAATG
TAACCGCGCACGTAAAGTTGTTATTACTGCAACTCAAATGCTCGATTCCATGATCAAAAACCCACGTCCTACCCGCGCAG
AAGCCGGTGACGTTGCAAACGCCATCCTTGACGGTACCGACGCGGTCATGCTGTCCGGCGAAAGTGCTAAGGGTAAGTAC
CCATTAGAGTCTGTCACCATCATGGCGACCATCTGTGAGCGTACAGACCGTGTTATGCCTAGCCGCATTGAGTCACTGAA
CGACAACCGTAAAATGCGAATCACTGAAGCCGTTTGCCGTGGTGCTGTTGAAACCGCAGAAAAACTGGAAGCCAAAGTGA
TTGTGGTCGCAACCGGGGGGGGTAAATCTGCAAAATCTGTCCGTAAATACTTCCCTACTGCGACCATTCTGGCACTGACG
ACCAATGAAATGACAGCCCATCAGTTAATTCTGACTAAAGGCGTAATCACTCAGTTGGTAGACGAAATCGCCTCAACCGA
TGATTTCTACCGTCTCGGTAAAGAAGCGGCTCTGGCGAGCGGCCTGGCACAGAAAGGCGACGTGGTCATCATGGTTTCAG
GTGCCTTGGTACCAAGCGGTACGACCAATACCTCTTCAGTACACGTGTTATAA

Upstream 100 bases:

>100_bases
CACGTTTCGCTACACCCAACACCAATCAGATACCACTCCGTTGTTGTAAATAACGGAACTTTCACTTTTTATCCATCACA
ACTTGTAGAAAAAATCCGTC

Downstream 100 bases:

>100_bases
TTCCAACGCGTTATTGTTCAATAATTAAAACGCCGCTCTCTATAGCGGCGTTTTCTTTTTTAAAGCTTGCCTAATAAACT
TTCTGAAAAAATTTTACTTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKTKIVCTIGPKTESEEMLTNLLNAGMNVMRLNFSHGDYAEHGQRIKNLRAVMAKTGKKAAILLDTKGPEIRTMKLEGG
KDAALVAGQTFTFTTDQSVVGNDKIVAVTYPGFAADLKIGNTVLVDDGLIGMEVIEVTEHTVVCKVLNSGDLGENKGVNL
PGVSIQLPALAEKDKGDLIFGCEQGVDFVAASFIRKRSDVLEIREHLKAHGGEHIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLESVTIMATICERTDRVMPSRIESLNDNRKMRITEAVCRGAVETAEKLEAKVIVVATGGGKSAKSVRKYFPTATILALT
TNEMTAHQLILTKGVITQLVDEIASTDDFYRLGKEAALASGLAQKGDVVIMVSGALVPSGTTNTSSVHVL

Sequences:

>Translated_470_residues
MKKTKIVCTIGPKTESEEMLTNLLNAGMNVMRLNFSHGDYAEHGQRIKNLRAVMAKTGKKAAILLDTKGPEIRTMKLEGG
KDAALVAGQTFTFTTDQSVVGNDKIVAVTYPGFAADLKIGNTVLVDDGLIGMEVIEVTEHTVVCKVLNSGDLGENKGVNL
PGVSIQLPALAEKDKGDLIFGCEQGVDFVAASFIRKRSDVLEIREHLKAHGGEHIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLESVTIMATICERTDRVMPSRIESLNDNRKMRITEAVCRGAVETAEKLEAKVIVVATGGGKSAKSVRKYFPTATILALT
TNEMTAHQLILTKGVITQLVDEIASTDDFYRLGKEAALASGLAQKGDVVIMVSGALVPSGTTNTSSVHVL
>Mature_470_residues
MKKTKIVCTIGPKTESEEMLTNLLNAGMNVMRLNFSHGDYAEHGQRIKNLRAVMAKTGKKAAILLDTKGPEIRTMKLEGG
KDAALVAGQTFTFTTDQSVVGNDKIVAVTYPGFAADLKIGNTVLVDDGLIGMEVIEVTEHTVVCKVLNSGDLGENKGVNL
PGVSIQLPALAEKDKGDLIFGCEQGVDFVAASFIRKRSDVLEIREHLKAHGGEHIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLESVTIMATICERTDRVMPSRIESLNDNRKMRITEAVCRGAVETAEKLEAKVIVVATGGGKSAKSVRKYFPTATILALT
TNEMTAHQLILTKGVITQLVDEIASTDDFYRLGKEAALASGLAQKGDVVIMVSGALVPSGTTNTSSVHVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=47.3251028806584, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=47.3251028806584, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=47.0225872689938, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI10835121, Length=486, Percent_Identity=46.2962962962963, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI32967597, Length=486, Percent_Identity=46.2962962962963, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=50, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=50, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=44.4915254237288, Blast_Score=185, Evalue=9e-47,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=44.4915254237288, Blast_Score=185, Evalue=9e-47,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=44.1860465116279, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=86.5957446808511, Blast_Score=834, Evalue=0.0,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=37.7593360995851, Blast_Score=270, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17544584, Length=476, Percent_Identity=42.2268907563025, Blast_Score=354, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI17506829, Length=480, Percent_Identity=45.2083333333333, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI71984413, Length=480, Percent_Identity=45.2083333333333, Blast_Score=339, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17506831, Length=480, Percent_Identity=45.2083333333333, Blast_Score=339, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI71984406, Length=480, Percent_Identity=45.2083333333333, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=44.0082644628099, Blast_Score=351, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6324923, Length=480, Percent_Identity=43.75, Blast_Score=344, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=47.239263803681, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=47.239263803681, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24648966, Length=446, Percent_Identity=46.6367713004484, Blast_Score=341, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24581235, Length=481, Percent_Identity=34.5114345114345, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24646914, Length=274, Percent_Identity=47.0802919708029, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50482; Mature: 50482

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPKTESEEMLTNLLNAGMNVMRLNFSHGDYAEHGQRIKNLRAVMAKTGKK
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCC
AAILLDTKGPEIRTMKLEGGKDAALVAGQTFTFTTDQSVVGNDKIVAVTYPGFAADLKIG
EEEEEECCCCCEEEEEECCCCCEEEEECCEEEEECCCCCCCCCEEEEEECCCEEEEEECC
NTVLVDDGLIGMEVIEVTEHTVVCKVLNSGDLGENKGVNLPGVSIQLPALAEKDKGDLIF
CEEEEECCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEE
GCEQGVDFVAASFIRKRSDVLEIREHLKAHGGEHIQIISKIENQEGLNNFDEILEASDGI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPLESVTIMATICERTDRVMPSRIESLNDNRKMRITEAVCR
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHHHH
GAVETAEKLEAKVIVVATGGGKSAKSVRKYFPTATILALTTNEMTAHQLILTKGVITQLV
HHHHHHHHCCEEEEEEEECCCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
DEIASTDDFYRLGKEAALASGLAQKGDVVIMVSGALVPSGTTNTSSVHVL
HHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEECEECCCCCCCCCEEEEC
>Mature Secondary Structure
MKKTKIVCTIGPKTESEEMLTNLLNAGMNVMRLNFSHGDYAEHGQRIKNLRAVMAKTGKK
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCC
AAILLDTKGPEIRTMKLEGGKDAALVAGQTFTFTTDQSVVGNDKIVAVTYPGFAADLKIG
EEEEEECCCCCEEEEEECCCCCEEEEECCEEEEECCCCCCCCCEEEEEECCCEEEEEECC
NTVLVDDGLIGMEVIEVTEHTVVCKVLNSGDLGENKGVNLPGVSIQLPALAEKDKGDLIF
CEEEEECCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEE
GCEQGVDFVAASFIRKRSDVLEIREHLKAHGGEHIQIISKIENQEGLNNFDEILEASDGI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPLESVTIMATICERTDRVMPSRIESLNDNRKMRITEAVCR
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHHHH
GAVETAEKLEAKVIVVATGGGKSAKSVRKYFPTATILALTTNEMTAHQLILTKGVITQLV
HHHHHHHHCCEEEEEEEECCCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
DEIASTDDFYRLGKEAALASGLAQKGDVVIMVSGALVPSGTTNTSSVHVL
HHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEECEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]