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The map label for this gene is ackA [H]

Identifier: 170023792

GI number: 170023792

Start: 1713682

End: 1714884

Strand: Reverse

Name: ackA [H]

Synonym: YPK_1551

Alternate gene names: 170023792

Gene position: 1714884-1713682 (Counterclockwise)

Preceding gene: 170023798

Following gene: 170023791

Centisome position: 36.57

GC content: 50.54

Gene sequence:

>1203_bases
ATGTCGAGTAAGCTAGTACTGGTTCTTAACTGCGGTAGCTCCTCCCTGAAATTTGCAATCATCGATGCGACCAACGGTGA
AGAGCATATTTCTGGTTTAGCCGAATGTTTCCATCTGCCAGAAGCCCGTATCAAATGGAAAGTTGACGGCGGTAAGCAGG
AAGCAGCATTGGGTGCTGGTGCAGCACACAGCGAAGCACTGAATTTTATTGTTAATACTATTTTGGCACAAAAACCAGCG
CTTTCTGCTCAGTTGACGGCCATCGGTCACCGTATCGTTCACGGCGGCGAGAAATTCACTTCGTCAGTTATCGTTACCGA
CGATGTTATTCAGGGTATCAAAGATTCTATCCCGTTTGCACCACTACACAACCCTGCCCACTTGATCGGTATTGCTGAAG
CCTTGAAATCCTTCCCTAATCTGGCGGATAAAAATGTGGCCGTCTTTGACACCGCGTTCCATCAGACCATGCCGGAAGAG
TCTTATCTCTATGCTCTGCCGTACAGCCTGTATAAAGATCACGGTATCCGCCGCTATGGCGCACACGGTACCAGCCACTT
CTATGTTAGCCAAGAAGCCGCCAAGATCTTGAACAAGCCGTTGGAAGAACTGAACGTGATTACCTGCCATTTGGGCAACG
GTGGTTCAGTAACCGCAGTGCGTAACGGTAAATGTGTCGATACGTCAATGGGGCTAACGCCACTGGAAGGTCTGGTGATG
GGGACCCGCAGTGGTGATCTCGATCCCGCTATTATCTTCCATCTGCATGATGCTATGGGCATGAGCGTTGATCAAATCAA
CACGTTGCTGACCAAAGAGTCAGGCCTGTTGGGGCTGACTGAAGTCACCAGCGACTGCCGTTATGTTGAAGATAACTACG
CCACCAAAGCTGACGCGAAACGTGCGATGGATGTGTTCTGTCACCGTTTGGCAAAATATATCGGTTCCTATACCGCGCTG
ATGGATGGTCGTCTGGATGCGGTCGTCTTCACCGGTGGTATTGGTGAAAATGCTGCCATGGTCCGTGAACTGACGCTGGA
CAAATTGGGCCTGCTGGGCTTTGAGATTGACCACGAACGTAACATGGCAGCACGCTTTGGCAAGTCAGGTACCATCACCA
AAGATAGCAGCCGTCTGGCTCTGGTTATCCCAACCAACGAAGAGTTGGTTATCGCGCAAGACGCAGCCCGTCTGACTGCG
TAA

Upstream 100 bases:

>100_bases
TTAACTCCGTATTTTTCACTGTTTTTGTGGAGTCGATGGGACAATACGCATCGGTCTTGCAAAAAACAAATGTTAAACGC
GACTATACATAGGTACTTCC

Downstream 100 bases:

>100_bases
ATAATACTTCTATACCCAAAGTCATTGGCGTTGTAGGTAGGCTGCGAACGAATCCCGATGAGTCTCTTCATTTTAAGGTT
AACGACCCGTAAATGATTCG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSSKLVLVLNCGSSSLKFAIIDATNGEEHISGLAECFHLPEARIKWKVDGGKQEAALGAGAAHSEALNFIVNTILAQKPA
LSAQLTAIGHRIVHGGEKFTSSVIVTDDVIQGIKDSIPFAPLHNPAHLIGIAEALKSFPNLADKNVAVFDTAFHQTMPEE
SYLYALPYSLYKDHGIRRYGAHGTSHFYVSQEAAKILNKPLEELNVITCHLGNGGSVTAVRNGKCVDTSMGLTPLEGLVM
GTRSGDLDPAIIFHLHDAMGMSVDQINTLLTKESGLLGLTEVTSDCRYVEDNYATKADAKRAMDVFCHRLAKYIGSYTAL
MDGRLDAVVFTGGIGENAAMVRELTLDKLGLLGFEIDHERNMAARFGKSGTITKDSSRLALVIPTNEELVIAQDAARLTA

Sequences:

>Translated_400_residues
MSSKLVLVLNCGSSSLKFAIIDATNGEEHISGLAECFHLPEARIKWKVDGGKQEAALGAGAAHSEALNFIVNTILAQKPA
LSAQLTAIGHRIVHGGEKFTSSVIVTDDVIQGIKDSIPFAPLHNPAHLIGIAEALKSFPNLADKNVAVFDTAFHQTMPEE
SYLYALPYSLYKDHGIRRYGAHGTSHFYVSQEAAKILNKPLEELNVITCHLGNGGSVTAVRNGKCVDTSMGLTPLEGLVM
GTRSGDLDPAIIFHLHDAMGMSVDQINTLLTKESGLLGLTEVTSDCRYVEDNYATKADAKRAMDVFCHRLAKYIGSYTAL
MDGRLDAVVFTGGIGENAAMVRELTLDKLGLLGFEIDHERNMAARFGKSGTITKDSSRLALVIPTNEELVIAQDAARLTA
>Mature_399_residues
SSKLVLVLNCGSSSLKFAIIDATNGEEHISGLAECFHLPEARIKWKVDGGKQEAALGAGAAHSEALNFIVNTILAQKPAL
SAQLTAIGHRIVHGGEKFTSSVIVTDDVIQGIKDSIPFAPLHNPAHLIGIAEALKSFPNLADKNVAVFDTAFHQTMPEES
YLYALPYSLYKDHGIRRYGAHGTSHFYVSQEAAKILNKPLEELNVITCHLGNGGSVTAVRNGKCVDTSMGLTPLEGLVMG
TRSGDLDPAIIFHLHDAMGMSVDQINTLLTKESGLLGLTEVTSDCRYVEDNYATKADAKRAMDVFCHRLAKYIGSYTALM
DGRLDAVVFTGGIGENAAMVRELTLDKLGLLGFEIDHERNMAARFGKSGTITKDSSRLALVIPTNEELVIAQDAARLTA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=88.25, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=45.2020202020202, Blast_Score=324, Evalue=7e-90,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43005; Mature: 42873

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLVLVLNCGSSSLKFAIIDATNGEEHISGLAECFHLPEARIKWKVDGGKQEAALGAG
CCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCCC
AAHSEALNFIVNTILAQKPALSAQLTAIGHRIVHGGEKFTSSVIVTDDVIQGIKDSIPFA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCEEEEEHHHHHHHHHCCCCC
PLHNPAHLIGIAEALKSFPNLADKNVAVFDTAFHQTMPEESYLYALPYSLYKDHGIRRYG
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEEECHHHHHCCCCEEEC
AHGTSHFYVSQEAAKILNKPLEELNVITCHLGNGGSVTAVRNGKCVDTSMGLTPLEGLVM
CCCCEEEEECHHHHHHHHCCHHHCCEEEEEECCCCCEEEEECCEEEECCCCCCCCCCEEE
GTRSGDLDPAIIFHLHDAMGMSVDQINTLLTKESGLLGLTEVTSDCRYVEDNYATKADAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RAMDVFCHRLAKYIGSYTALMDGRLDAVVFTGGIGENAAMVRELTLDKLGLLGFEIDHER
HHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCC
NMAARFGKSGTITKDSSRLALVIPTNEELVIAQDAARLTA
CHHHHCCCCCCEEECCCEEEEEEECCCCEEEEECHHHCCC
>Mature Secondary Structure 
SSKLVLVLNCGSSSLKFAIIDATNGEEHISGLAECFHLPEARIKWKVDGGKQEAALGAG
CCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCCC
AAHSEALNFIVNTILAQKPALSAQLTAIGHRIVHGGEKFTSSVIVTDDVIQGIKDSIPFA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCEEEEEHHHHHHHHHCCCCC
PLHNPAHLIGIAEALKSFPNLADKNVAVFDTAFHQTMPEESYLYALPYSLYKDHGIRRYG
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEEECHHHHHCCCCEEEC
AHGTSHFYVSQEAAKILNKPLEELNVITCHLGNGGSVTAVRNGKCVDTSMGLTPLEGLVM
CCCCEEEEECHHHHHHHHCCHHHCCEEEEEECCCCCEEEEECCEEEECCCCCCCCCCEEE
GTRSGDLDPAIIFHLHDAMGMSVDQINTLLTKESGLLGLTEVTSDCRYVEDNYATKADAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RAMDVFCHRLAKYIGSYTALMDGRLDAVVFTGGIGENAAMVRELTLDKLGLLGFEIDHER
HHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCC
NMAARFGKSGTITKDSSRLALVIPTNEELVIAQDAARLTA
CHHHHCCCCCCEEECCCEEEEEEECCCCEEEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA