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The map label for this gene is napA [H]

Identifier: 170023629

GI number: 170023629

Start: 1528809

End: 1531301

Strand: Direct

Name: napA [H]

Synonym: YPK_1387

Alternate gene names: 170023629

Gene position: 1528809-1531301 (Clockwise)

Preceding gene: 170023628

Following gene: 170023630

Centisome position: 32.6

GC content: 53.15

Gene sequence:

>2493_bases
ATGAAACTCAGTCGCCGGGACTTTATGAAAGCGAATGCGGCCGTTGCTGCGGCGGCTGCCGCCGGAATGACCATACCTAC
TGTCGCTAAAGCGGTGGGTGAGACAACCAATGCGATCAAGTGGGATAAAGCACCTTGCCGATTCTGCGGCACCGGCTGCG
GTGTACTGGTAGGAACGCAAAATGGCCGTATCGTGGCCTCACAAGGTGACCCGGACTCACCGGTTAACCGTGGGCTGAAC
TGCATCAAAGGCTATTTCCTGCCAAAAATCATGTACGGCAAAGACCGGCTGACACAGCCCTTGCTGCGTATGAAAGACGG
TCAATACGATAAAGAAGGCGATTTCACCCCAATAAGCTGGGAGAAAGCCTTTGATATCATGGAACTGAAATTCAAAAATG
CGCTAAAAGAGAAAGGCCCGACCGCGGTCGGTATGTTCGGCTCCGGGCAATGGACCGTGTGGGAAGGCTACGCGGCGTTG
AAGTTGCTGAAAGGGGGGTTCCGCTCAAATAACCTTGATCCTAATGCCCGCCATTGTATGGCGTCCTCGGTTGTTGGATT
CATGCGTACCTTCGGTATGGATGAGCCGATGGGGTGCTACGATGATATTGAAGAAGCCGATGCCTTCGTGCTCTGGGGCT
CCAATATGGCGGAAATGCACCCGGTATTATGGTCGCGTATGACCAGCCGCCGCCTGACCAATGCGCATGTCAGAATTGCC
GTCCTCTCCACTTACGAACACCGCAGTTTTGAATTGGCCGACAACCCGATCGTCTTTACCCCACAAACCGATCTGGTCAT
CATGAATTACATCGCCAATTACATCATTCAAAATAATGCCGTTGATAAAGACTTCCTGGCTCAACACGTGAATTTCCGCC
GCGGCGCGACCGATATCGGCTATGGCTTACGGCCAACCCATCCGTTGGAAAAAGCGGCGAAGAATCCCGGCAGCGATGCT
TCTGAACCGATGAGTTTTGAGGATTTCAAAACCTTTGTCGCTGAATACACGTTAGAAAAAACCGCCAAAATGAGCGGTGT
ACCAGAAGATCAGCTTGAGTCGTTGGCCCAGTTGTATGCTGATCCAAAGGTGAAATTGGTCTCTTACTGGACCATGGGCT
TTAACCAGCATACCCGCGGCGTGTGGGCCAACAACATGTGCTACAACCTGCACCTGTTAACCGGCAAGATCTCCACGCCG
GGATCGGGGCCTTTCTCCCTGACGGGGCAGCCTTCCGCGTGTGGCACCGCCCGCGAAGTGGGGACATTCTCCCATCGCCT
GCCTGCAGATATGGTGGTCACGAACGAAAAACATCGGCAGATTGCTGAAACCACATGGCAGTTACCGGCGGGGACCATCC
CGGAAAAAGTGGGTTTACATGCGGTAGCACAAGATCGGGCGCTGAAAGACGGCACCCTCAACGCCTACTGGGTGATGTGC
AACAACAACATGCAGGCCGGACCGAATATTAATGAAGAGCGTATGCCGGGCTGGCGTGATCCGCGCAACTTTATTGTGGT
CTCCGATCCCTATCCCACCATCAGTGCGCTGTCTGCTGACTTGATTTTACCGACCTCAATGTGGGTCGAGAAAGAGGGCG
CATACGGCAATGCTGAACGCCGTACTCAATTCTGGCGTCAGCAAGTCCCCTCACCGGGGGAGGCTAAATCGGATTTATGG
CAAATCGTCGAGTTCGCCAAACGCTTTAACGTCGAAGAAGTCTGGCCCGCTGAGTTGGTGAATCAAAAACCTGAGTATCG
CGGTAAAAATTTATATGAGGTGCTGTTTGCCAACGATGTAGTCAGTAAATACCCACTGAGCGAGATCCCTGACGATCAAT
TGAACGACGAAGCGCGCGATTTTGGTTTCTACATACAGAAAGGATTATTTGAAGAGTACGCCAGCTTTGGGCGTGGGCAC
GCTCATGATCTGGCTCCTTTCGATGTATATCATCAGGTACGCGGCCTGCGCTGGCCGGTGGTTGACGGTAAGGAAACACT
CTGGCGCTACCGTGAAGGTTTTGATCCCTTCGTACCGAAAGGCGAAGAGGTGCGCTTCTATGGCAAACCAGACGGTAAGG
CGGTCATTTTTGCCCTGCCTTATGAACCAGCAGCAGAAAGCCCGGACCAAGAATATGACCTGTGGCTCTCTACCGGCCGG
GTGCTGGAACATTGGCACACGGGTTCAATGACCCGCCGGGTACCTGAATTGCACCGTGCTTTCCCAGAGGCCGTGTTATT
CATTCATCCATTGGATGCCAAAGCGCGTGGTTTACACCGTGGTGACAAAGTGAAAGTGATTTCACGCCGAGGTGAGGTTA
TTTCTCTGGTTGAAACCCGTGGCCGTAACCGCCCACCGCGAGGGCTGGTGTACATGCCGTTCTTCGATGCCGCACAGTTG
GTCAATAACCTGACCTTAGACGCGACCGATCCGCTCTCGAAAGAAACTGACTTTAAGAAATGCGCAGTGAAACTGGAACG
GGTAGTGGCCTGA

Upstream 100 bases:

>100_bases
ACACGCTCCTGAATCATATTGAGTCAGCGCGTAATCTGGATGGTGTGCTGGCTGTATCGCTGGTTTATCACCAGCAGGAT
TTGCAAGGTGAGGAAATGCC

Downstream 100 bases:

>100_bases
TGATTCACAGATAACCATTAATTACAGATAACCATCATCCGGGAATGCCAAGGAAAAAGACGGTGTCTTTTGGGAGTAAA
TAATGAATAACACAATGCTT

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 830; Mature: 830

Protein sequence:

>830_residues
MKLSRRDFMKANAAVAAAAAAGMTIPTVAKAVGETTNAIKWDKAPCRFCGTGCGVLVGTQNGRIVASQGDPDSPVNRGLN
CIKGYFLPKIMYGKDRLTQPLLRMKDGQYDKEGDFTPISWEKAFDIMELKFKNALKEKGPTAVGMFGSGQWTVWEGYAAL
KLLKGGFRSNNLDPNARHCMASSVVGFMRTFGMDEPMGCYDDIEEADAFVLWGSNMAEMHPVLWSRMTSRRLTNAHVRIA
VLSTYEHRSFELADNPIVFTPQTDLVIMNYIANYIIQNNAVDKDFLAQHVNFRRGATDIGYGLRPTHPLEKAAKNPGSDA
SEPMSFEDFKTFVAEYTLEKTAKMSGVPEDQLESLAQLYADPKVKLVSYWTMGFNQHTRGVWANNMCYNLHLLTGKISTP
GSGPFSLTGQPSACGTAREVGTFSHRLPADMVVTNEKHRQIAETTWQLPAGTIPEKVGLHAVAQDRALKDGTLNAYWVMC
NNNMQAGPNINEERMPGWRDPRNFIVVSDPYPTISALSADLILPTSMWVEKEGAYGNAERRTQFWRQQVPSPGEAKSDLW
QIVEFAKRFNVEEVWPAELVNQKPEYRGKNLYEVLFANDVVSKYPLSEIPDDQLNDEARDFGFYIQKGLFEEYASFGRGH
AHDLAPFDVYHQVRGLRWPVVDGKETLWRYREGFDPFVPKGEEVRFYGKPDGKAVIFALPYEPAAESPDQEYDLWLSTGR
VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARGLHRGDKVKVISRRGEVISLVETRGRNRPPRGLVYMPFFDAAQL
VNNLTLDATDPLSKETDFKKCAVKLERVVA

Sequences:

>Translated_830_residues
MKLSRRDFMKANAAVAAAAAAGMTIPTVAKAVGETTNAIKWDKAPCRFCGTGCGVLVGTQNGRIVASQGDPDSPVNRGLN
CIKGYFLPKIMYGKDRLTQPLLRMKDGQYDKEGDFTPISWEKAFDIMELKFKNALKEKGPTAVGMFGSGQWTVWEGYAAL
KLLKGGFRSNNLDPNARHCMASSVVGFMRTFGMDEPMGCYDDIEEADAFVLWGSNMAEMHPVLWSRMTSRRLTNAHVRIA
VLSTYEHRSFELADNPIVFTPQTDLVIMNYIANYIIQNNAVDKDFLAQHVNFRRGATDIGYGLRPTHPLEKAAKNPGSDA
SEPMSFEDFKTFVAEYTLEKTAKMSGVPEDQLESLAQLYADPKVKLVSYWTMGFNQHTRGVWANNMCYNLHLLTGKISTP
GSGPFSLTGQPSACGTAREVGTFSHRLPADMVVTNEKHRQIAETTWQLPAGTIPEKVGLHAVAQDRALKDGTLNAYWVMC
NNNMQAGPNINEERMPGWRDPRNFIVVSDPYPTISALSADLILPTSMWVEKEGAYGNAERRTQFWRQQVPSPGEAKSDLW
QIVEFAKRFNVEEVWPAELVNQKPEYRGKNLYEVLFANDVVSKYPLSEIPDDQLNDEARDFGFYIQKGLFEEYASFGRGH
AHDLAPFDVYHQVRGLRWPVVDGKETLWRYREGFDPFVPKGEEVRFYGKPDGKAVIFALPYEPAAESPDQEYDLWLSTGR
VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARGLHRGDKVKVISRRGEVISLVETRGRNRPPRGLVYMPFFDAAQL
VNNLTLDATDPLSKETDFKKCAVKLERVVA
>Mature_830_residues
MKLSRRDFMKANAAVAAAAAAGMTIPTVAKAVGETTNAIKWDKAPCRFCGTGCGVLVGTQNGRIVASQGDPDSPVNRGLN
CIKGYFLPKIMYGKDRLTQPLLRMKDGQYDKEGDFTPISWEKAFDIMELKFKNALKEKGPTAVGMFGSGQWTVWEGYAAL
KLLKGGFRSNNLDPNARHCMASSVVGFMRTFGMDEPMGCYDDIEEADAFVLWGSNMAEMHPVLWSRMTSRRLTNAHVRIA
VLSTYEHRSFELADNPIVFTPQTDLVIMNYIANYIIQNNAVDKDFLAQHVNFRRGATDIGYGLRPTHPLEKAAKNPGSDA
SEPMSFEDFKTFVAEYTLEKTAKMSGVPEDQLESLAQLYADPKVKLVSYWTMGFNQHTRGVWANNMCYNLHLLTGKISTP
GSGPFSLTGQPSACGTAREVGTFSHRLPADMVVTNEKHRQIAETTWQLPAGTIPEKVGLHAVAQDRALKDGTLNAYWVMC
NNNMQAGPNINEERMPGWRDPRNFIVVSDPYPTISALSADLILPTSMWVEKEGAYGNAERRTQFWRQQVPSPGEAKSDLW
QIVEFAKRFNVEEVWPAELVNQKPEYRGKNLYEVLFANDVVSKYPLSEIPDDQLNDEARDFGFYIQKGLFEEYASFGRGH
AHDLAPFDVYHQVRGLRWPVVDGKETLWRYREGFDPFVPKGEEVRFYGKPDGKAVIFALPYEPAAESPDQEYDLWLSTGR
VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARGLHRGDKVKVISRRGEVISLVETRGRNRPPRGLVYMPFFDAAQL
VNNLTLDATDPLSKETDFKKCAVKLERVVA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=828, Percent_Identity=82.7294685990338, Blast_Score=1475, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=557, Percent_Identity=25.3141831238779, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI3868720, Length=660, Percent_Identity=23.6363636363636, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI87081797, Length=876, Percent_Identity=24.5433789954338, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI3868719, Length=663, Percent_Identity=22.6244343891403, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 93257; Mature: 93257

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRRDFMKANAAVAAAAAAGMTIPTVAKAVGETTNAIKWDKAPCRFCGTGCGVLVGTQ
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEECCCCHHHHCCCCEEEEECC
NGRIVASQGDPDSPVNRGLNCIKGYFLPKIMYGKDRLTQPLLRMKDGQYDKEGDFTPISW
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCH
EKAFDIMELKFKNALKEKGPTAVGMFGSGQWTVWEGYAALKLLKGGFRSNNLDPNARHCM
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECHHHHHHHHHCCCCCCCCCCHHHHHH
ASSVVGFMRTFGMDEPMGCYDDIEEADAFVLWGSNMAEMHPVLWSRMTSRRLTNAHVRIA
HHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEE
VLSTYEHRSFELADNPIVFTPQTDLVIMNYIANYIIQNNAVDKDFLAQHVNFRRGATDIG
EEEECCCCCEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
YGLRPTHPLEKAAKNPGSDASEPMSFEDFKTFVAEYTLEKTAKMSGVPEDQLESLAQLYA
CCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPKVKLVSYWTMGFNQHTRGVWANNMCYNLHLLTGKISTPGSGPFSLTGQPSACGTAREV
CCCEEEEEEEECCCCCCCCCEEECCEEEEEEEEEEECCCCCCCCEEECCCCCCCCCHHHH
GTFSHRLPADMVVTNEKHRQIAETTWQLPAGTIPEKVGLHAVAQDRALKDGTLNAYWVMC
CCHHHCCCCCEEEECHHHHHHHHHHHCCCCCCCCHHHCCHHHHHCCCCCCCCCEEEEEEE
NNNMQAGPNINEERMPGWRDPRNFIVVSDPYPTISALSADLILPTSMWVEKEGAYGNAER
CCCCCCCCCCCHHCCCCCCCCCCEEEECCCCCCHHHHCCCCEECCHHEEECCCCCCCHHH
RTQFWRQQVPSPGEAKSDLWQIVEFAKRFNVEEVWPAELVNQKPEYRGKNLYEVLFANDV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEHHHHHHH
VSKYPLSEIPDDQLNDEARDFGFYIQKGLFEEYASFGRGHAHDLAPFDVYHQVRGLRWPV
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCC
VDGKETLWRYREGFDPFVPKGEEVRFYGKPDGKAVIFALPYEPAAESPDQEYDLWLSTGR
CCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCHHEEECCCH
VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARGLHRGDKVKVISRRGEVISLVETR
HHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCEEEEECCCCEEEEHHHC
GRNRPPRGLVYMPFFDAAQLVNNLTLDATDPLSKETDFKKCAVKLERVVA
CCCCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLSRRDFMKANAAVAAAAAAGMTIPTVAKAVGETTNAIKWDKAPCRFCGTGCGVLVGTQ
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEECCCCHHHHCCCCEEEEECC
NGRIVASQGDPDSPVNRGLNCIKGYFLPKIMYGKDRLTQPLLRMKDGQYDKEGDFTPISW
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCH
EKAFDIMELKFKNALKEKGPTAVGMFGSGQWTVWEGYAALKLLKGGFRSNNLDPNARHCM
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECHHHHHHHHHCCCCCCCCCCHHHHHH
ASSVVGFMRTFGMDEPMGCYDDIEEADAFVLWGSNMAEMHPVLWSRMTSRRLTNAHVRIA
HHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEE
VLSTYEHRSFELADNPIVFTPQTDLVIMNYIANYIIQNNAVDKDFLAQHVNFRRGATDIG
EEEECCCCCEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
YGLRPTHPLEKAAKNPGSDASEPMSFEDFKTFVAEYTLEKTAKMSGVPEDQLESLAQLYA
CCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPKVKLVSYWTMGFNQHTRGVWANNMCYNLHLLTGKISTPGSGPFSLTGQPSACGTAREV
CCCEEEEEEEECCCCCCCCCEEECCEEEEEEEEEEECCCCCCCCEEECCCCCCCCCHHHH
GTFSHRLPADMVVTNEKHRQIAETTWQLPAGTIPEKVGLHAVAQDRALKDGTLNAYWVMC
CCHHHCCCCCEEEECHHHHHHHHHHHCCCCCCCCHHHCCHHHHHCCCCCCCCCEEEEEEE
NNNMQAGPNINEERMPGWRDPRNFIVVSDPYPTISALSADLILPTSMWVEKEGAYGNAER
CCCCCCCCCCCHHCCCCCCCCCCEEEECCCCCCHHHHCCCCEECCHHEEECCCCCCCHHH
RTQFWRQQVPSPGEAKSDLWQIVEFAKRFNVEEVWPAELVNQKPEYRGKNLYEVLFANDV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEHHHHHHH
VSKYPLSEIPDDQLNDEARDFGFYIQKGLFEEYASFGRGHAHDLAPFDVYHQVRGLRWPV
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCC
VDGKETLWRYREGFDPFVPKGEEVRFYGKPDGKAVIFALPYEPAAESPDQEYDLWLSTGR
CCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCHHEEECCCH
VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARGLHRGDKVKVISRRGEVISLVETR
HHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCEEEEECCCCEEEEHHHC
GRNRPPRGLVYMPFFDAAQLVNNLTLDATDPLSKETDFKKCAVKLERVVA
CCCCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA