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The map label for this gene is mlaE [H]

Identifier: 170022764

GI number: 170022764

Start: 564161

End: 564943

Strand: Direct

Name: mlaE [H]

Synonym: YPK_0513

Alternate gene names: 170022764

Gene position: 564161-564943 (Clockwise)

Preceding gene: 170022763

Following gene: 170022765

Centisome position: 12.03

GC content: 50.57

Gene sequence:

>783_bases
ATGTTAGTAAAGTCGTTAGCATCTTTAGGTCGCCGAGGAATTAATGTTTGCGCCTCATTTGGTCGCGCAGGCTTGATGCT
GTTCAATGCCCTTGTCGGGCGGCCTGAACCGCGCAAACAATGGCCATTATTAATCAAACAACTGTACAGCGTTGGGGTTC
AATCCCTGTTGATTATCGTGGTCTCCGGTTTGTTCATCGGTATGGTTCTGGGTTTACAGGGCTTTTTGATCCTCACCACG
TACAGCGCAGAGGCCAGCCTCGGCATGATGGTTTCTTTATCACTGCTGCGTGAATTGGGGCCTGTGGTCACTGCGTTACT
GTTTGCGGGGCGAGCTGGTTCTGCCCTGACCGCAGAAATTGGCCTAATGAAAGCCACTGAACAGATCTCAAGTCTGGAAA
TGATGGCTATCGACCCTTTACGGCGAGTGGTTGCGCCTCGCTTCTGGGCCGGTCTGATCAGTATGCCATTGCTGACAGCT
ATTTTTGTTGCTGTGGGTATTTGGGGCGGTTCAGTGGTTGGCGTTGATTGGAAAGGGATCGACAGTGGTTTCTTCTGGTC
AGCGATGCAAAATGCGGTGGAGTGGCGTACAGATTTACTGAATTGCCTGATTAAGAGTCTGGTATTTGCTCTTACCGTGA
CCTGGATTGCGCTGTTCAATGGTTATGATGCGGTCCCAACGTCTGAAGGGATTAGCCGGGCAACGACACGTACTGTAGTG
CATTCGTCACTGGCGGTATTGGGATTGGATTTTGTGCTGACAGCACTGATGTTTGGGAACTGA

Upstream 100 bases:

>100_bases
CTATAAGTAAAATCTACTACAGGTAATGGATTCAGGTGAGTGAACATAGCGAATCCCCCTATAGCATCAAACACTAAGGG
GATGGGCTGATGGAGTATTC

Downstream 100 bases:

>100_bases
GTCGATGCAAACGAAGAAAAGTGAAGTCTGGGTAGGAATGTTTATACTGATTGCTATTCTGGCAGTCGTATTTCTCTGCC
TAAAAGTTGCAGATATCAAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLVKSLASLGRRGINVCASFGRAGLMLFNALVGRPEPRKQWPLLIKQLYSVGVQSLLIIVVSGLFIGMVLGLQGFLILTT
YSAEASLGMMVSLSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQISSLEMMAIDPLRRVVAPRFWAGLISMPLLTA
IFVAVGIWGGSVVGVDWKGIDSGFFWSAMQNAVEWRTDLLNCLIKSLVFALTVTWIALFNGYDAVPTSEGISRATTRTVV
HSSLAVLGLDFVLTALMFGN

Sequences:

>Translated_260_residues
MLVKSLASLGRRGINVCASFGRAGLMLFNALVGRPEPRKQWPLLIKQLYSVGVQSLLIIVVSGLFIGMVLGLQGFLILTT
YSAEASLGMMVSLSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQISSLEMMAIDPLRRVVAPRFWAGLISMPLLTA
IFVAVGIWGGSVVGVDWKGIDSGFFWSAMQNAVEWRTDLLNCLIKSLVFALTVTWIALFNGYDAVPTSEGISRATTRTVV
HSSLAVLGLDFVLTALMFGN
>Mature_260_residues
MLVKSLASLGRRGINVCASFGRAGLMLFNALVGRPEPRKQWPLLIKQLYSVGVQSLLIIVVSGLFIGMVLGLQGFLILTT
YSAEASLGMMVSLSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQISSLEMMAIDPLRRVVAPRFWAGLISMPLLTA
IFVAVGIWGGSVVGVDWKGIDSGFFWSAMQNAVEWRTDLLNCLIKSLVFALTVTWIALFNGYDAVPTSEGISRATTRTVV
HSSLAVLGLDFVLTALMFGN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=260, Percent_Identity=82.6923076923077, Blast_Score=441, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27884; Mature: 27884

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVKSLASLGRRGINVCASFGRAGLMLFNALVGRPEPRKQWPLLIKQLYSVGVQSLLIIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VSGLFIGMVLGLQGFLILTTYSAEASLGMMVSLSLLRELGPVVTALLFAGRAGSALTAEI
HHHHHHHHHHHHCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLMKATEQISSLEMMAIDPLRRVVAPRFWAGLISMPLLTAIFVAVGIWGGSVVGVDWKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
DSGFFWSAMQNAVEWRTDLLNCLIKSLVFALTVTWIALFNGYDAVPTSEGISRATTRTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
HSSLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLVKSLASLGRRGINVCASFGRAGLMLFNALVGRPEPRKQWPLLIKQLYSVGVQSLLIIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VSGLFIGMVLGLQGFLILTTYSAEASLGMMVSLSLLRELGPVVTALLFAGRAGSALTAEI
HHHHHHHHHHHHCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLMKATEQISSLEMMAIDPLRRVVAPRFWAGLISMPLLTAIFVAVGIWGGSVVGVDWKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
DSGFFWSAMQNAVEWRTDLLNCLIKSLVFALTVTWIALFNGYDAVPTSEGISRATTRTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
HSSLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]