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The map label for this gene is pmbA [H]

Identifier: 170022741

GI number: 170022741

Start: 542505

End: 543845

Strand: Direct

Name: pmbA [H]

Synonym: YPK_0490

Alternate gene names: 170022741

Gene position: 542505-543845 (Clockwise)

Preceding gene: 170022739

Following gene: 170022742

Centisome position: 11.57

GC content: 52.95

Gene sequence:

>1341_bases
ATGAAAGTAGTCACTCAAGTTGCAGAACAGCGTAAAACGCTGGAACAAGCTGTTGCACAGGCTTTGGAATTAGCCCGCGC
GGGTTCTGATGCGGCCGAAGTCGCCGTTAGCAAAACCACCGGGATCAGCGTCAGCACTCGCTTTGGTGAAGTGGAAAACG
TGGAGTTCAACAGTGATGGTGCGCTGGGAATTACCGTTTACCACCAACAGCGTAAAGGCAGTGCGTCCACCACGGATTTA
AATCCGGATGCCGTTGCCCGCACCGTGCAGGCGGCATTGGATATTGCCCGTTATACGTCACCCGACCCCTACGCCGGACC
TGCAGACAAATCTCTGCTGGCCTTTGATGCACCAGATTTGGATTTATTCCATCCTAGCGATCTGGATGCTGAGCAAGGTA
TCTTGCTGGCGGCCCGCGCAGAACAGGCGGCATTGCAGGCTGACAAGCGGATCACCAATACCGAAGGGGGGAGTTTTAAC
AGCCATTATGGCATCAAAGTCTTCGGTAACAGCCACGGTATGTTGCAAAGCTACTGCTCCAGTCGTCACTCGCTCTCGAG
CAGTGTGATTGCCGAGCACAATGGGGATATGGAGCGTGATTACGCCTATACCATTGGCCGTCGGATGGAGGATTTGGCTA
CGCCAGAATGGGTCGGTGAGGAGTGTGCGCGTCGTACTCTATCTCGTCTGTCTCCGCGTAAATTACCGACCATGCAGTCA
CCCGTGCTGTTTGCGGCTGAAGTAGCCACTGGCCTGTTTGGTCATCTGGTGTCAGCGATCAGCGGCGGTAATATTTATCG
CAAATCGACGTTCTTGCTCGATCATTTAGGCCAACAAATTTTACCTGAATGGTTGACCATTGAAGAGCATCCACATCTCT
TGCGTGGTCTGGCATCAACACCGTTCGACAGTGAAGGCGTGCGCACACTGCAACGTGAAATCGTCAAAGACGGGGTGCTG
CAAACTTACTTGTTGACCAGTTATTCTGCCCGTAAATTGGGGCTGCAAAGCACCGGGCACGCTGGTGGCATCCATAACTG
GCGCATTGCCGGTCAGGGCCAGGATTTCGCGGGTATGCTGAAGCAACTGGATAAAGGTTTGGTCGTCACCGAATTGATGG
GCCAGGGCGTTAGTACGGTTACCGGGGATTATTCCCGAGGTGCTGCGGGTTTCTGGGTCGAAAATGGCGAGATTCAGTAC
CCGGTCAGTGAGATAACCATCGCGGGTAATCTGAAAGATATGCTACGCAATATCGTCAGTGTGGGCAGTGATATCGAAAC
TCGCAGCAATATTCAGTGCGGCTCCGTTTTATTGCCCGTTATGAAAATCGCGGGCGAATAA

Upstream 100 bases:

>100_bases
CAGATCCATGGCGTTCTTTATTTTATCCGTATAAGTCGTCGTATTACTACGCCGCCACGGCACTTTTCTGAACGATTATC
TCATTAATTATGGCAGATCG

Downstream 100 bases:

>100_bases
TAACCTTCCTGTCATCTTACTGTTCCACAACTCGCGGGTAGAAAATACCCGCGAGTTTGCCTGCGCTTTTCCTTGATATG
ATAGTCTTCCCCAGTTTGTA

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKVVTQVAEQRKTLEQAVAQALELARAGSDAAEVAVSKTTGISVSTRFGEVENVEFNSDGALGITVYHQQRKGSASTTDL
NPDAVARTVQAALDIARYTSPDPYAGPADKSLLAFDAPDLDLFHPSDLDAEQGILLAARAEQAALQADKRITNTEGGSFN
SHYGIKVFGNSHGMLQSYCSSRHSLSSSVIAEHNGDMERDYAYTIGRRMEDLATPEWVGEECARRTLSRLSPRKLPTMQS
PVLFAAEVATGLFGHLVSAISGGNIYRKSTFLLDHLGQQILPEWLTIEEHPHLLRGLASTPFDSEGVRTLQREIVKDGVL
QTYLLTSYSARKLGLQSTGHAGGIHNWRIAGQGQDFAGMLKQLDKGLVVTELMGQGVSTVTGDYSRGAAGFWVENGEIQY
PVSEITIAGNLKDMLRNIVSVGSDIETRSNIQCGSVLLPVMKIAGE

Sequences:

>Translated_446_residues
MKVVTQVAEQRKTLEQAVAQALELARAGSDAAEVAVSKTTGISVSTRFGEVENVEFNSDGALGITVYHQQRKGSASTTDL
NPDAVARTVQAALDIARYTSPDPYAGPADKSLLAFDAPDLDLFHPSDLDAEQGILLAARAEQAALQADKRITNTEGGSFN
SHYGIKVFGNSHGMLQSYCSSRHSLSSSVIAEHNGDMERDYAYTIGRRMEDLATPEWVGEECARRTLSRLSPRKLPTMQS
PVLFAAEVATGLFGHLVSAISGGNIYRKSTFLLDHLGQQILPEWLTIEEHPHLLRGLASTPFDSEGVRTLQREIVKDGVL
QTYLLTSYSARKLGLQSTGHAGGIHNWRIAGQGQDFAGMLKQLDKGLVVTELMGQGVSTVTGDYSRGAAGFWVENGEIQY
PVSEITIAGNLKDMLRNIVSVGSDIETRSNIQCGSVLLPVMKIAGE
>Mature_446_residues
MKVVTQVAEQRKTLEQAVAQALELARAGSDAAEVAVSKTTGISVSTRFGEVENVEFNSDGALGITVYHQQRKGSASTTDL
NPDAVARTVQAALDIARYTSPDPYAGPADKSLLAFDAPDLDLFHPSDLDAEQGILLAARAEQAALQADKRITNTEGGSFN
SHYGIKVFGNSHGMLQSYCSSRHSLSSSVIAEHNGDMERDYAYTIGRRMEDLATPEWVGEECARRTLSRLSPRKLPTMQS
PVLFAAEVATGLFGHLVSAISGGNIYRKSTFLLDHLGQQILPEWLTIEEHPHLLRGLASTPFDSEGVRTLQREIVKDGVL
QTYLLTSYSARKLGLQSTGHAGGIHNWRIAGQGQDFAGMLKQLDKGLVVTELMGQGVSTVTGDYSRGAAGFWVENGEIQY
PVSEITIAGNLKDMLRNIVSVGSDIETRSNIQCGSVLLPVMKIAGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=446, Percent_Identity=82.7354260089686, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI1789640, Length=408, Percent_Identity=20.343137254902, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48135; Mature: 48135

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVTQVAEQRKTLEQAVAQALELARAGSDAAEVAVSKTTGISVSTRFGEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCEEEECCCCCCCEEECCCC
ALGITVYHQQRKGSASTTDLNPDAVARTVQAALDIARYTSPDPYAGPADKSLLAFDAPDL
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
DLFHPSDLDAEQGILLAARAEQAALQADKRITNTEGGSFNSHYGIKVFGNSHGMLQSYCS
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHH
SRHSLSSSVIAEHNGDMERDYAYTIGRRMEDLATPEWVGEECARRTLSRLSPRKLPTMQS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
PVLFAAEVATGLFGHLVSAISGGNIYRKSTFLLDHLGQQILPEWLTIEEHPHLLRGLAST
CHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
PFDSEGVRTLQREIVKDGVLQTYLLTSYSARKLGLQSTGHAGGIHNWRIAGQGQDFAGML
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHH
KQLDKGLVVTELMGQGVSTVTGDYSRGAAGFWVENGEIQYPVSEITIAGNLKDMLRNIVS
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEECHHHEEEECCHHHHHHHHHH
VGSDIETRSNIQCGSVLLPVMKIAGE
CCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVVTQVAEQRKTLEQAVAQALELARAGSDAAEVAVSKTTGISVSTRFGEVENVEFNSDG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCEEEECCCCCCCEEECCCC
ALGITVYHQQRKGSASTTDLNPDAVARTVQAALDIARYTSPDPYAGPADKSLLAFDAPDL
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
DLFHPSDLDAEQGILLAARAEQAALQADKRITNTEGGSFNSHYGIKVFGNSHGMLQSYCS
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHH
SRHSLSSSVIAEHNGDMERDYAYTIGRRMEDLATPEWVGEECARRTLSRLSPRKLPTMQS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
PVLFAAEVATGLFGHLVSAISGGNIYRKSTFLLDHLGQQILPEWLTIEEHPHLLRGLAST
CHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
PFDSEGVRTLQREIVKDGVLQTYLLTSYSARKLGLQSTGHAGGIHNWRIAGQGQDFAGML
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHH
KQLDKGLVVTELMGQGVSTVTGDYSRGAAGFWVENGEIQYPVSEITIAGNLKDMLRNIVS
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEECHHHEEEECCHHHHHHHHHH
VGSDIETRSNIQCGSVLLPVMKIAGE
CCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]