Click here to switch to the map view.

The map label for this gene is aceA [H]

Identifier: 170022620

GI number: 170022620

Start: 393317

End: 394624

Strand: Direct

Name: aceA [H]

Synonym: YPK_0365

Alternate gene names: 170022620

Gene position: 393317-394624 (Clockwise)

Preceding gene: 170022619

Following gene: 170022621

Centisome position: 8.39

GC content: 51.38

Gene sequence:

>1308_bases
ATGACGATCTCTCGTACTCAACAAATTCAGCAGTTGGAACAGGAATGGACATCGCCGCGCTGGAAAAATATCACTCGGCC
TTATAGCGCCGAAGATGTCATCAAGCTGCGTGGTTCGGTCAATCCAGAATGTACGTTCGCACAAAATGGTGCCAAAAAAC
TGTGGGAATTGCTGCACGGCGGGTCGCGTAAAGGGTATATCAACTGTCTGGGGGCATTAACCGGTGGTCAGGCGCTACAA
CAGGCGAAAGCCGGTGTTGAAGCCATTTATCTGTCGGGATGGCAGGTGGCGGCTGATGCTAATACGGCATCTAGCATGTA
TCCAGATCAGTCACTGTATCCGGTCGACTCTGTGCCTGCGGTGGTGAAACGTATCAACAATAGCTTCCGCCGCGCTGATC
AAATTCAGTGGTCGAATAATATTGAGCCAGGTAGTAAAGGCTATACCGACTATTTCCTACCGATCGTCGCGGATGCGGAA
GCGGGTTTTGGCGGTGTGCTGAACGCGTTTGAATTAATGAAAGCGATGATTGAAGCGGGGGCGGCAGGGGTTCATTTTGA
AGACCAATTGGCCGCAGTGAAAAAGTGTGGGCATATGGGCGGTAAGGTACTGGTGCCGACACAAGAGGCCATTCAAAAGC
TGGTTGCTGCCCGTTTAGCCGCAGATGTCTTGGGTGTACCAACGCTGTTGATTGCCAGGACTGATGCGGATGCGGCTGAT
TTATTGACCTCAGATTGCGACCCCTATGACCGGGAGTTTATTACCGGCGACCGTACTGCTGAAGGTTTTTTCCGCACCCG
TGCCGGTATTGAACAAGCGATCAGCCGTGGCCTGGCGTATGCCCCTTATGCTGATTTGGTGTGGTGTGAAACCTCGACAC
CTGATCTGGCTCTGGCCAAACGTTTTGCCGATGCTGTTCATGCTCAATTCCCCGGAAAATTACTGGCTTATAACTGTTCA
CCCTCCTTTAACTGGAAGAAGAATCTGACCGACCAGCAAATTGCCAGCTTCCAGGATGAACTCTCCGCCATGGGCTACAA
ATACCAGTTTATTACGCTGGCGGGTATTCATAGCATGTGGTTCAACATGTTCGATCTGGCCCATGCGTATGCGCAGGGTG
AAGGCATGAAACACTACGTTGAGAAAGTCCAGCAACCTGAGTTTGCTTCTGTGGACCGCGGCTACACCTTTGCTTCCCAT
CAGCAAGAAGTGGGGACTGGCTACTTCGATAAAGTCACCAATATTATTCAGGGGGGCGCATCATCTGTAACGGCACTGAC
CGGTTCGACGGAAGAGCAGCAGTTTTAG

Upstream 100 bases:

>100_bases
CAAGACGAGCTTATCGACTTCCTGACGTTGCCGGGCTATGCATTGCTTGCCTGAGCCATCCTATTGAACCCTACAAAATT
GAATTATAAGGAACATCGCC

Downstream 100 bases:

>100_bases
CCACGATTGAGTGAGTGTGGTCTTATCAGGAGCCTGCTTATGCAGGCTCAGACTGCTGACAAACCCCGATGACGCGATCT
TGAACGGTGAGGATAGGCAG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTISRTQQIQQLEQEWTSPRWKNITRPYSAEDVIKLRGSVNPECTFAQNGAKKLWELLHGGSRKGYINCLGALTGGQALQ
QAKAGVEAIYLSGWQVAADANTASSMYPDQSLYPVDSVPAVVKRINNSFRRADQIQWSNNIEPGSKGYTDYFLPIVADAE
AGFGGVLNAFELMKAMIEAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIQKLVAARLAADVLGVPTLLIARTDADAAD
LLTSDCDPYDREFITGDRTAEGFFRTRAGIEQAISRGLAYAPYADLVWCETSTPDLALAKRFADAVHAQFPGKLLAYNCS
PSFNWKKNLTDQQIASFQDELSAMGYKYQFITLAGIHSMWFNMFDLAHAYAQGEGMKHYVEKVQQPEFASVDRGYTFASH
QQEVGTGYFDKVTNIIQGGASSVTALTGSTEEQQF

Sequences:

>Translated_435_residues
MTISRTQQIQQLEQEWTSPRWKNITRPYSAEDVIKLRGSVNPECTFAQNGAKKLWELLHGGSRKGYINCLGALTGGQALQ
QAKAGVEAIYLSGWQVAADANTASSMYPDQSLYPVDSVPAVVKRINNSFRRADQIQWSNNIEPGSKGYTDYFLPIVADAE
AGFGGVLNAFELMKAMIEAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIQKLVAARLAADVLGVPTLLIARTDADAAD
LLTSDCDPYDREFITGDRTAEGFFRTRAGIEQAISRGLAYAPYADLVWCETSTPDLALAKRFADAVHAQFPGKLLAYNCS
PSFNWKKNLTDQQIASFQDELSAMGYKYQFITLAGIHSMWFNMFDLAHAYAQGEGMKHYVEKVQQPEFASVDRGYTFASH
QQEVGTGYFDKVTNIIQGGASSVTALTGSTEEQQF
>Mature_434_residues
TISRTQQIQQLEQEWTSPRWKNITRPYSAEDVIKLRGSVNPECTFAQNGAKKLWELLHGGSRKGYINCLGALTGGQALQQ
AKAGVEAIYLSGWQVAADANTASSMYPDQSLYPVDSVPAVVKRINNSFRRADQIQWSNNIEPGSKGYTDYFLPIVADAEA
GFGGVLNAFELMKAMIEAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIQKLVAARLAADVLGVPTLLIARTDADAADL
LTSDCDPYDREFITGDRTAEGFFRTRAGIEQAISRGLAYAPYADLVWCETSTPDLALAKRFADAVHAQFPGKLLAYNCSP
SFNWKKNLTDQQIASFQDELSAMGYKYQFITLAGIHSMWFNMFDLAHAYAQGEGMKHYVEKVQQPEFASVDRGYTFASHQ
QEVGTGYFDKVTNIIQGGASSVTALTGSTEEQQF

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=432, Percent_Identity=85.4166666666667, Blast_Score=786, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=265, Percent_Identity=29.4339622641509, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17561814, Length=420, Percent_Identity=58.3333333333333, Blast_Score=487, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=58.3333333333333, Blast_Score=487, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6320908, Length=295, Percent_Identity=36.271186440678, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6325263, Length=253, Percent_Identity=39.1304347826087, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 47654; Mature: 47523

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISRTQQIQQLEQEWTSPRWKNITRPYSAEDVIKLRGSVNPECTFAQNGAKKLWELLHG
CCCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHCCCCCCCEEECCCHHHHHHHHHHC
GSRKGYINCLGALTGGQALQQAKAGVEAIYLSGWQVAADANTASSMYPDQSLYPVDSVPA
CCCCCHHHHHHHHHCHHHHHHHHCCCEEEEECCEEEEECCCCHHHCCCCCCCCCCHHHHH
VVKRINNSFRRADQIQWSNNIEPGSKGYTDYFLPIVADAEAGFGGVLNAFELMKAMIEAG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
AAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIQKLVAARLAADVLGVPTLLIARTDADAAD
CCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHH
LLTSDCDPYDREFITGDRTAEGFFRTRAGIEQAISRGLAYAPYADLVWCETSTPDLALAK
HHHCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHH
RFADAVHAQFPGKLLAYNCSPSFNWKKNLTDQQIASFQDELSAMGYKYQFITLAGIHSMW
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHH
FNMFDLAHAYAQGEGMKHYVEKVQQPEFASVDRGYTFASHQQEVGTGYFDKVTNIIQGGA
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHCCC
SSVTALTGSTEEQQF
CCEEEECCCCCCCCC
>Mature Secondary Structure 
TISRTQQIQQLEQEWTSPRWKNITRPYSAEDVIKLRGSVNPECTFAQNGAKKLWELLHG
CCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHCCCCCCCEEECCCHHHHHHHHHHC
GSRKGYINCLGALTGGQALQQAKAGVEAIYLSGWQVAADANTASSMYPDQSLYPVDSVPA
CCCCCHHHHHHHHHCHHHHHHHHCCCEEEEECCEEEEECCCCHHHCCCCCCCCCCHHHHH
VVKRINNSFRRADQIQWSNNIEPGSKGYTDYFLPIVADAEAGFGGVLNAFELMKAMIEAG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
AAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIQKLVAARLAADVLGVPTLLIARTDADAAD
CCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHH
LLTSDCDPYDREFITGDRTAEGFFRTRAGIEQAISRGLAYAPYADLVWCETSTPDLALAK
HHHCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHH
RFADAVHAQFPGKLLAYNCSPSFNWKKNLTDQQIASFQDELSAMGYKYQFITLAGIHSMW
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHH
FNMFDLAHAYAQGEGMKHYVEKVQQPEFASVDRGYTFASHQQEVGTGYFDKVTNIIQGGA
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHCCC
SSVTALTGSTEEQQF
CCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]