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The map label for this gene is estB [H]

Identifier: 169629265

GI number: 169629265

Start: 2192827

End: 2193975

Strand: Direct

Name: estB [H]

Synonym: MAB_2179

Alternate gene names: 169629265

Gene position: 2192827-2193975 (Clockwise)

Preceding gene: 169629264

Following gene: 169629269

Centisome position: 43.28

GC content: 65.45

Gene sequence:

>1149_bases
ATGGGTAGCGATTCCACACTCGCAGAACGTCTTGACGAGGTCTTTGGCGCCGCACTGCGGGAACGCCGGGTCGTGGGCGC
TGTGGCCGTCGTCGCACGTGATGGCCAGGTCCAATACCGTCGCGCCCATGGCCTCGCGGACCGCGAAGGATCCCTGCCGA
TGCGCGAGGACACGCTGTTCCGGCTTGCCTCGGTGACCAAGCCGATCGTGACTGTGGCGGTGCTGCGGCTTGTCGGCGAT
GAGCTCATCCGGCTCGATGATCCCGTGACGCGCTGGCTTCCCGACTTCACGCCGAGGCTTCCCGACGGCAGCACCCCGGC
GCTAACCATTCATCACTTACTTACTCACACAGCGGGTTTGAGCTATGGGTTGCTGGAGGAACCCGACTCGCCATTCCATG
CTCTGGGCATATCGGACGGCATCGATGTGGTCGACTTCGATCTGCGGGAGAATCTGAGGCGCCTCGCCTTGGCGCCCCTA
CAATTCGCGCCGGGCAGTGCCTGGCGGTATTCGTTGGCACTCGACGTGCTGGGCGCGGTGGTGGAGAACGCTACCGGTCT
GCCGCTGGATCTGGCGGTGGAGAAACTGGTCACCTCCCCACTGGGCATGCACGAGTCGGGGTTTGTGGCCCGTGACCTGA
CCCGCTTTGCGGTGCCCTATGCCAATGGCGAACCCGAACCGTTTCGGATCGCCGACAACGTCAATGTGCCGCTACCGCAA
GGGATCGGTGTCGCAGTGCGTTTCGGCCCGGCGCGGGTGACGCGCGGCGACGCATATCCCTCCGGCGGTGCCGGGATGTA
CGGCACCGCCGATGATGTCCTGCTCCTGTTGGAAGCGATCCGTGCCGGCGACGGCTTCCTGGCGGAGGAGCTACGCACGT
CCATGCGGACCGACCACGTGGGCCCGCAGGCCCAAACCAGAGGGCCAGGATGGGGATTCGGCTACGGCGGTGCGGTATTG
GCGGACCCGGCAGCTGCCGACAGTCCCCAGAGTGCGGGAACAATCGGCTGGGGCGGAGTCTATGGGCACAGCTGGTTTGT
GGATGCCGCGCGCGGATTGTCGGTGCTGCTACTGACGAACACCGCCTACGAGGGTATGACGGGTCCATTGACCGTCGAGA
TGCGGGACGCGGTGTACGCGGCGGACTAG

Upstream 100 bases:

>100_bases
CGGTATCGATCCCGATGATCTACGCCGCTCGCCGGAGTATGCCCAATTGCGCACCGAGGTCGGCCGGGCCGTGAAGGCCG
CGGCCGCCTAGAGTTTCTCC

Downstream 100 bases:

>100_bases
TTCTCCGCACGGTTACGCAGCAGCTCCGCCGCAGTCAGCTGATAGGCATGTTCGGCCCATTCCAATGGTGTTGTCCCATA
CCGTAAGTCGGTGATCGTGG

Product: Beta-lactamase/esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MGSDSTLAERLDEVFGAALRERRVVGAVAVVARDGQVQYRRAHGLADREGSLPMREDTLFRLASVTKPIVTVAVLRLVGD
ELIRLDDPVTRWLPDFTPRLPDGSTPALTIHHLLTHTAGLSYGLLEEPDSPFHALGISDGIDVVDFDLRENLRRLALAPL
QFAPGSAWRYSLALDVLGAVVENATGLPLDLAVEKLVTSPLGMHESGFVARDLTRFAVPYANGEPEPFRIADNVNVPLPQ
GIGVAVRFGPARVTRGDAYPSGGAGMYGTADDVLLLLEAIRAGDGFLAEELRTSMRTDHVGPQAQTRGPGWGFGYGGAVL
ADPAAADSPQSAGTIGWGGVYGHSWFVDAARGLSVLLLTNTAYEGMTGPLTVEMRDAVYAAD

Sequences:

>Translated_382_residues
MGSDSTLAERLDEVFGAALRERRVVGAVAVVARDGQVQYRRAHGLADREGSLPMREDTLFRLASVTKPIVTVAVLRLVGD
ELIRLDDPVTRWLPDFTPRLPDGSTPALTIHHLLTHTAGLSYGLLEEPDSPFHALGISDGIDVVDFDLRENLRRLALAPL
QFAPGSAWRYSLALDVLGAVVENATGLPLDLAVEKLVTSPLGMHESGFVARDLTRFAVPYANGEPEPFRIADNVNVPLPQ
GIGVAVRFGPARVTRGDAYPSGGAGMYGTADDVLLLLEAIRAGDGFLAEELRTSMRTDHVGPQAQTRGPGWGFGYGGAVL
ADPAAADSPQSAGTIGWGGVYGHSWFVDAARGLSVLLLTNTAYEGMTGPLTVEMRDAVYAAD
>Mature_381_residues
GSDSTLAERLDEVFGAALRERRVVGAVAVVARDGQVQYRRAHGLADREGSLPMREDTLFRLASVTKPIVTVAVLRLVGDE
LIRLDDPVTRWLPDFTPRLPDGSTPALTIHHLLTHTAGLSYGLLEEPDSPFHALGISDGIDVVDFDLRENLRRLALAPLQ
FAPGSAWRYSLALDVLGAVVENATGLPLDLAVEKLVTSPLGMHESGFVARDLTRFAVPYANGEPEPFRIADNVNVPLPQG
IGVAVRFGPARVTRGDAYPSGGAGMYGTADDVLLLLEAIRAGDGFLAEELRTSMRTDHVGPQAQTRGPGWGFGYGGAVLA
DPAAADSPQSAGTIGWGGVYGHSWFVDAARGLSVLLLTNTAYEGMTGPLTVEMRDAVYAAD

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=364, Percent_Identity=25.8241758241758, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786574, Length=184, Percent_Identity=29.3478260869565, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI115533026, Length=353, Percent_Identity=23.7960339943343, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: NA

Molecular weight: Translated: 40675; Mature: 40543

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSDSTLAERLDEVFGAALRERRVVGAVAVVARDGQVQYRRAHGLADREGSLPMREDTLF
CCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEHHHCCCCCCCCCCCCCHHHHH
RLASVTKPIVTVAVLRLVGDELIRLDDPVTRWLPDFTPRLPDGSTPALTIHHLLTHTAGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
SYGLLEEPDSPFHALGISDGIDVVDFDLRENLRRLALAPLQFAPGSAWRYSLALDVLGAV
CCCCCCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHH
VENATGLPLDLAVEKLVTSPLGMHESGFVARDLTRFAVPYANGEPEPFRIADNVNVPLPQ
HHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCC
GIGVAVRFGPARVTRGDAYPSGGAGMYGTADDVLLLLEAIRAGDGFLAEELRTSMRTDHV
CCEEEEEECCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
GPQAQTRGPGWGFGYGGAVLADPAAADSPQSAGTIGWGGVYGHSWFVDAARGLSVLLLTN
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEC
TAYEGMTGPLTVEMRDAVYAAD
CCCCCCCCCEEEEECCCEEECC
>Mature Secondary Structure 
GSDSTLAERLDEVFGAALRERRVVGAVAVVARDGQVQYRRAHGLADREGSLPMREDTLF
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEHHHCCCCCCCCCCCCCHHHHH
RLASVTKPIVTVAVLRLVGDELIRLDDPVTRWLPDFTPRLPDGSTPALTIHHLLTHTAGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
SYGLLEEPDSPFHALGISDGIDVVDFDLRENLRRLALAPLQFAPGSAWRYSLALDVLGAV
CCCCCCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHH
VENATGLPLDLAVEKLVTSPLGMHESGFVARDLTRFAVPYANGEPEPFRIADNVNVPLPQ
HHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCC
GIGVAVRFGPARVTRGDAYPSGGAGMYGTADDVLLLLEAIRAGDGFLAEELRTSMRTDHV
CCEEEEEECCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
GPQAQTRGPGWGFGYGGAVLADPAAADSPQSAGTIGWGGVYGHSWFVDAARGLSVLLLTN
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEC
TAYEGMTGPLTVEMRDAVYAAD
CCCCCCCCCEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]