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The map label for this gene is dop [H]

Identifier: 169629256

GI number: 169629256

Start: 2184891

End: 2186387

Strand: Direct

Name: dop [H]

Synonym: MAB_2170

Alternate gene names: 169629256

Gene position: 2184891-2186387 (Clockwise)

Preceding gene: 169629254

Following gene: 169629257

Centisome position: 43.12

GC content: 65.8

Gene sequence:

>1497_bases
ATGCAACGGATCATCGGTACCGAGGTTGAGTACGGCATTTCGTCGCCCACGGACCCCGCCGCCAATCCGATCCTCACGTC
GACGCAGGCGGTGCTGGCGTACGCCGCGGCAACGGGGATTCAGCGTGCCAAGCGCACCCGCTGGGACTATGAGGTGGAGT
CGCCGCTGCGCGACGCTCGCGGATTCGACCTGGGGCGTACCACCGGCCCGGCGCCGATCGTCGATGCCGACGAGGTGGGG
GCGGCCAACATGATCTTGACCAACGGGGCCCGCCTGTACGTCGATCACGCGCACCCGGAGTACTCGGCACCCGAGTGCAC
CAATCCATTGGACGCCGTCATCTGGGACAAGGCCGGAGAGCGCGTCATGGAGGCGGCGGCGCGCCACGTGGCCAGTGTGC
CGGGCGCGGCACGGCTACAGCTGTACAAGAACAACATCGACGGCAAGGGTGCCTCTTACGGCACTCATGAGAACTATTTG
ATGGATCGTCAGACACCGTTCTCGGCGATTATTGCCGGGCTGACCCCGTTCTTCGTCTCGCGCCAGGTCGTCACCGGGTC
TGGCCGCGTGGGCATTGGGCCTTCCGGTGACGATGCGGGCTTCCAGCTTTCCCAGCGTGCCGACTACATCGAGGTCGAGG
TGGGTCTGGAGACAACACTCAAGCGCGGCATCATCAATACCCGCGACGAGCCGCACGCCGACGCCGACAAGTACCGGCGC
CTGCACGTCATCATCGGTGACGCCAACCTTGCCGAGACCTCGACATTCCTGAAGGTGGGCACCACCTCACTGGTGCTCGA
CCTGATCGAGTCCGGTGTGGACCTGTCGGATCTGGCATTGGCCCGCCCGGTCCATGCGGTGCACGTGATCAGTCGCGACC
CCTCCCTGCGGGCCACCGTCGCGCTTGCCGACGGTCGGGAGCTGACCGGGCTTGCACTGCAACGCATTTACCTGGACCGG
GTGGCCGAGCTGGTGTCCTCGCGTGACCCGGATCCCCAGGCCGACCAGGTGCTCCAGGTGTGGGCCAAGGTGCTTGATCT
GCTCGAGCGCGACCCGATGGAATGTGCGGACCTGCTGGACTGGCCCGCGAAGCTGCGGCTGCTGGAGGGGTTCCGGCAGC
GCGAGAACCTGAGCTGGTCGGCGCCGCGTCTGCACCTGGTAGACCTGCAGTACTCCGACGTCCGGCTGGATAAGGGGCTC
TACAACAGGCTTGTCGCCCGCGGCTCCATGCAGCGGATGGTCACCGAGCAGCAGGTGCTGGACGCGGTGACCCGGCCTCC
CTCGGACACCCGGGCCTACTTCCGTGGCGAGTGTCTGCGCAAATTCGGCGCCGATATCGCCGCCGCGAGCTGGGACTCGG
TGATTTTCGACCTGGGCGGGGATTCGCTTGTCAGGATTCCAACGCTGGAGCCGCTGCGCGGAAGCAAGAGTCACGTCGGC
GCGCTGCTGGATTCTGTCGATTCAGCCGCTGAACTGGTCGATCAGCTCACCACGTAA

Upstream 100 bases:

>100_bases
TGGCTCATGGACGCGACGCTAATGTCGTTTTCGGGGCACGCGCAGGACTCTGAACGGTCCCCGGCGTGGTGTTCAGCGTC
GCCTCTCTAGGCTGAACACC

Downstream 100 bases:

>100_bases
CCAGTTGGGCGACGCACACTTGTGAAACGTCACCTCGTCGGCGTTGCCCGGTAGGCTGAACAGAACCGACCTATGAGTCA
GGAGGCAGCGATGGCGCAGG

Product: hypothetical protein

Products: NA

Alternate protein names: Deamidase of protein Pup [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MQRIIGTEVEYGISSPTDPAANPILTSTQAVLAYAAATGIQRAKRTRWDYEVESPLRDARGFDLGRTTGPAPIVDADEVG
AANMILTNGARLYVDHAHPEYSAPECTNPLDAVIWDKAGERVMEAAARHVASVPGAARLQLYKNNIDGKGASYGTHENYL
MDRQTPFSAIIAGLTPFFVSRQVVTGSGRVGIGPSGDDAGFQLSQRADYIEVEVGLETTLKRGIINTRDEPHADADKYRR
LHVIIGDANLAETSTFLKVGTTSLVLDLIESGVDLSDLALARPVHAVHVISRDPSLRATVALADGRELTGLALQRIYLDR
VAELVSSRDPDPQADQVLQVWAKVLDLLERDPMECADLLDWPAKLRLLEGFRQRENLSWSAPRLHLVDLQYSDVRLDKGL
YNRLVARGSMQRMVTEQQVLDAVTRPPSDTRAYFRGECLRKFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKSHVG
ALLDSVDSAAELVDQLTT

Sequences:

>Translated_498_residues
MQRIIGTEVEYGISSPTDPAANPILTSTQAVLAYAAATGIQRAKRTRWDYEVESPLRDARGFDLGRTTGPAPIVDADEVG
AANMILTNGARLYVDHAHPEYSAPECTNPLDAVIWDKAGERVMEAAARHVASVPGAARLQLYKNNIDGKGASYGTHENYL
MDRQTPFSAIIAGLTPFFVSRQVVTGSGRVGIGPSGDDAGFQLSQRADYIEVEVGLETTLKRGIINTRDEPHADADKYRR
LHVIIGDANLAETSTFLKVGTTSLVLDLIESGVDLSDLALARPVHAVHVISRDPSLRATVALADGRELTGLALQRIYLDR
VAELVSSRDPDPQADQVLQVWAKVLDLLERDPMECADLLDWPAKLRLLEGFRQRENLSWSAPRLHLVDLQYSDVRLDKGL
YNRLVARGSMQRMVTEQQVLDAVTRPPSDTRAYFRGECLRKFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKSHVG
ALLDSVDSAAELVDQLTT
>Mature_498_residues
MQRIIGTEVEYGISSPTDPAANPILTSTQAVLAYAAATGIQRAKRTRWDYEVESPLRDARGFDLGRTTGPAPIVDADEVG
AANMILTNGARLYVDHAHPEYSAPECTNPLDAVIWDKAGERVMEAAARHVASVPGAARLQLYKNNIDGKGASYGTHENYL
MDRQTPFSAIIAGLTPFFVSRQVVTGSGRVGIGPSGDDAGFQLSQRADYIEVEVGLETTLKRGIINTRDEPHADADKYRR
LHVIIGDANLAETSTFLKVGTTSLVLDLIESGVDLSDLALARPVHAVHVISRDPSLRATVALADGRELTGLALQRIYLDR
VAELVSSRDPDPQADQVLQVWAKVLDLLERDPMECADLLDWPAKLRLLEGFRQRENLSWSAPRLHLVDLQYSDVRLDKGL
YNRLVARGSMQRMVTEQQVLDAVTRPPSDTRAYFRGECLRKFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKSHVG
ALLDSVDSAAELVDQLTT

Specific function: Specifically catalyzes the deamidation of the C-terminal glutamine of the prokaryotic ubiquitin-like protein Pup to glutamate, thereby rendering Pup competent for conjugation. Also displays depupylase (DPUP) activity, removing conjugated Pup from target p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup deamidase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022366
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 54386; Mature: 54386

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRIIGTEVEYGISSPTDPAANPILTSTQAVLAYAAATGIQRAKRTRWDYEVESPLRDAR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHC
GFDLGRTTGPAPIVDADEVGAANMILTNGARLYVDHAHPEYSAPECTNPLDAVIWDKAGE
CCCCCCCCCCCCCCCCHHCCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
RVMEAAARHVASVPGAARLQLYKNNIDGKGASYGTHENYLMDRQTPFSAIIAGLTPFFVS
HHHHHHHHHHHHCCCHHEEEEEECCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHH
RQVVTGSGRVGIGPSGDDAGFQLSQRADYIEVEVGLETTLKRGIINTRDEPHADADKYRR
CEEEECCCCCCCCCCCCCCCCEECCCCCEEEEEECHHHHHHHCCCCCCCCCCCCHHHCEE
LHVIIGDANLAETSTFLKVGTTSLVLDLIESGVDLSDLALARPVHAVHVISRDPSLRATV
EEEEEECCCHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCEEEE
ALADGRELTGLALQRIYLDRVAELVSSRDPDPQADQVLQVWAKVLDLLERDPMECADLLD
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHC
WPAKLRLLEGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMQRMVTEQQVL
CHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
DAVTRPPSDTRAYFRGECLRKFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKSHVG
HHHHCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCCCCCHHHHH
ALLDSVDSAAELVDQLTT
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQRIIGTEVEYGISSPTDPAANPILTSTQAVLAYAAATGIQRAKRTRWDYEVESPLRDAR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHC
GFDLGRTTGPAPIVDADEVGAANMILTNGARLYVDHAHPEYSAPECTNPLDAVIWDKAGE
CCCCCCCCCCCCCCCCHHCCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
RVMEAAARHVASVPGAARLQLYKNNIDGKGASYGTHENYLMDRQTPFSAIIAGLTPFFVS
HHHHHHHHHHHHCCCHHEEEEEECCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHH
RQVVTGSGRVGIGPSGDDAGFQLSQRADYIEVEVGLETTLKRGIINTRDEPHADADKYRR
CEEEECCCCCCCCCCCCCCCCEECCCCCEEEEEECHHHHHHHCCCCCCCCCCCCHHHCEE
LHVIIGDANLAETSTFLKVGTTSLVLDLIESGVDLSDLALARPVHAVHVISRDPSLRATV
EEEEEECCCHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCEEEE
ALADGRELTGLALQRIYLDRVAELVSSRDPDPQADQVLQVWAKVLDLLERDPMECADLLD
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHC
WPAKLRLLEGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMQRMVTEQQVL
CHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
DAVTRPPSDTRAYFRGECLRKFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKSHVG
HHHHCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCCCCCHHHHH
ALLDSVDSAAELVDQLTT
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]