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The map label for this gene is gph [C]

Identifier: 169629216

GI number: 169629216

Start: 2143546

End: 2144229

Strand: Direct

Name: gph [C]

Synonym: MAB_2130

Alternate gene names: 169629216

Gene position: 2143546-2144229 (Clockwise)

Preceding gene: 169629215

Following gene: 169629217

Centisome position: 42.3

GC content: 65.64

Gene sequence:

>684_bases
ATGAGAGCGGTGCTGTGGGATATGGACGGCACCCTCATCGAATCGGAGAAGCTGTGGGACATCTCGATGGGAGAGCTGTG
CCGCAAGCTAGGCGGTGAGATGACGCCGGAGCTGCGTACCGCACTGGTAGGCGGCGCCGCGGATGCCACCATGCGGATGA
TCTTCACCGCGCTGGAGCTGGAACCCGACCCCGTGCGGATGGCCAGTGAGAACGATTGGCTGCACCAGTACACCGGCGAG
TTGTTCGCCACCGGTCTCATCTGGTGCGATGGCGCCCAGGAGATGCTGGGGCAGCTTGCCGCGGAGCAGATGCCGATGGC
CCTGGTAACCAACACCATCAGGTCATTGACCGATCAGGCACTCAAGACCATTGGCACGCATTGGTTCACCGAGTCGGTCT
GTGGTGATGAGGTGCCCGCTGGCAAGCCGGCCCCCGATCCGTACCTGCGCGCCGCCGCGCTACTGGGCGTCGAGCCACGC
GATTGCCTGGCCATCGAAGATTCGGTGACCGGAGCAGCCGCTGCCGAGGCAGCGGGATGCGCGGTCCTGGTGGTGCCCAA
CCATGTCGAGGTGCCCGCCGGAGCGCGGCGCAGACATGCCGCCAGCTTGTCTGGCCTTTCCGTGCGCGACCTGCGGGATG
CCCACGCCGACGTGCTCGCCGCGACCACCTGCAAACCCATATGA

Upstream 100 bases:

>100_bases
CAGGAAGCGGAGCGCTGGCTCGGCCCGAACCTCGGCTACAACCCGGAGGACTGAGCACCAGCATCATGGGCGGCAGAGGA
CCTCTGACAGAATTGATCGC

Downstream 100 bases:

>100_bases
CTCGCCGGGCAGGGTCGTGACAGAATCGCCACCCGTGAAGACCTTCGATGAGCTGTTCGCTGAGCTCAGTGATCGCGCCA
AGACCCGCCCCGAGGGGAGC

Product: putative hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MRAVLWDMDGTLIESEKLWDISMGELCRKLGGEMTPELRTALVGGAADATMRMIFTALELEPDPVRMASENDWLHQYTGE
LFATGLIWCDGAQEMLGQLAAEQMPMALVTNTIRSLTDQALKTIGTHWFTESVCGDEVPAGKPAPDPYLRAAALLGVEPR
DCLAIEDSVTGAAAAEAAGCAVLVVPNHVEVPAGARRRHAASLSGLSVRDLRDAHADVLAATTCKPI

Sequences:

>Translated_227_residues
MRAVLWDMDGTLIESEKLWDISMGELCRKLGGEMTPELRTALVGGAADATMRMIFTALELEPDPVRMASENDWLHQYTGE
LFATGLIWCDGAQEMLGQLAAEQMPMALVTNTIRSLTDQALKTIGTHWFTESVCGDEVPAGKPAPDPYLRAAALLGVEPR
DCLAIEDSVTGAAAAEAAGCAVLVVPNHVEVPAGARRRHAASLSGLSVRDLRDAHADVLAATTCKPI
>Mature_227_residues
MRAVLWDMDGTLIESEKLWDISMGELCRKLGGEMTPELRTALVGGAADATMRMIFTALELEPDPVRMASENDWLHQYTGE
LFATGLIWCDGAQEMLGQLAAEQMPMALVTNTIRSLTDQALKTIGTHWFTESVCGDEVPAGKPAPDPYLRAAALLGVEPR
DCLAIEDSVTGAAAAEAAGCAVLVVPNHVEVPAGARRRHAASLSGLSVRDLRDAHADVLAATTCKPI

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Homo sapiens, GI197382691, Length=222, Percent_Identity=26.1261261261261, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789787, Length=202, Percent_Identity=27.7227722772277, Blast_Score=66, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116008157, Length=195, Percent_Identity=31.2820512820513, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17137324, Length=193, Percent_Identity=31.0880829015544, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI20129151, Length=194, Percent_Identity=27.8350515463918, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR000150
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24254; Mature: 24254

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVLWDMDGTLIESEKLWDISMGELCRKLGGEMTPELRTALVGGAADATMRMIFTALEL
CCEEEECCCCCEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHC
EPDPVRMASENDWLHQYTGELFATGLIWCDGAQEMLGQLAAEQMPMALVTNTIRSLTDQA
CCCCCEECCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LKTIGTHWFTESVCGDEVPAGKPAPDPYLRAAALLGVEPRDCLAIEDSVTGAAAAEAAGC
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHCCE
AVLVVPNHVEVPAGARRRHAASLSGLSVRDLRDAHADVLAATTCKPI
EEEEECCCEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRAVLWDMDGTLIESEKLWDISMGELCRKLGGEMTPELRTALVGGAADATMRMIFTALEL
CCEEEECCCCCEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHC
EPDPVRMASENDWLHQYTGELFATGLIWCDGAQEMLGQLAAEQMPMALVTNTIRSLTDQA
CCCCCEECCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LKTIGTHWFTESVCGDEVPAGKPAPDPYLRAAALLGVEPRDCLAIEDSVTGAAAAEAAGC
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHCCE
AVLVVPNHVEVPAGARRRHAASLSGLSVRDLRDAHADVLAATTCKPI
EEEEECCCEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA