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The map label for this gene is yhxA [H]

Identifier: 169628793

GI number: 169628793

Start: 1730378

End: 1731772

Strand: Direct

Name: yhxA [H]

Synonym: MAB_1703

Alternate gene names: 169628793

Gene position: 1730378-1731772 (Clockwise)

Preceding gene: 169628792

Following gene: 169628797

Centisome position: 34.15

GC content: 66.16

Gene sequence:

>1395_bases
ATGACTGACGTTCAGAATCACATCGGAATCGATCCCGCCAGCGCGCCGATCGAGGCTCCCGCGGCCGAGGCGCAGGTCCG
CGCCGACGACCGTGGGCACGTTTTCCACTCATGGTCCGCGCAGGCGATGATCGACCCGCTGCCGATCGCCGGTGGTTCGG
GCGCGACATTCTGGGACTACACCGGCAAGGAGTACCTGGACTTCGCCTCGCAGCTGGTCAATCTCAACCTGGGGCACCAG
CATCCGCGTCTGGTCGAATCGATCCAGCGTCAGGCCGGTCGGCTGGCCACCATTGCCCCCGCCTTCGCCAACGACGTGCG
CGGCGAGCTTGCCCGCCTGGTCGCCGAGCGCGCGCCCGGAACCCTGAACAAGGTGTTCTTCACCAACGGTGGCACCGAGG
CCGTCGAGCATGCGGTGCGCATGGCGCGCCACCACACCGGGCGCCGCAAAGTGCTTGCCGCATACCGCAGCTACCACGGC
GGCACCACCACCTCGATGTCGCTGACGGGTGAGCCGCGCCGCTGGGCCAACGACCCGGGTGACAACTCCGTGGTGCACTT
CTTCGGTCCGTATGCCTACCGTTCGGTGTTCCACTCCACCACGCCCGAGGAGGAAACCCAGAGGGCGCTGGAGCATCTGG
AGCAGGTCATCAACCTGGAGGGCCCGCCCACCATCGCCGCGATCATCCTGGAATCGGTGGTCGGCACCAACGGTGTGCTG
GTGCCCCCGCCGGGCTACCTGGCCGGCGTTCGGGAGATCTGCGACCGGTACGGCCTGGTGTTCATCGCCGACGAGGTAAT
GGTCGGCTTCGGCCGATTCGGGGAATGGTTCGCCATCAACGCCTTTGATGTCACCCCGGATCTCATCACCTTTGCCAAGG
GCATCAACTCCGGTTACGTCCCGCTGGGTGGTGTGGTGATCTCCGATGCCATCGCCACCAGCTTCGACCACCGCCCGTAC
CCGGGTGGGCTGACCTACTCCGGCCATCCATTGGCCTGCGCGGTGGGCGTCGAGACCATCAAGGTCTTCGAGGATGACCA
GATCCTGAAGCGGGTGCGCGATCTCGGTGAGCGCGTGGTGCGTCCGGAGCTGGAACGCTGGGTGCAGGCGCACCCGAGCG
TCGGTGAGATCCGCGGCCGTGGCCTGTTCTGGGCCGTGGAACTGGTGCGCAACAAGGAAACTCGTGAACCCCTGGTGCCG
TTCAACGCCAGTGGTGAGGCGGCGGCGCCGATGAACGCGTTCGCGGCGGCGTGCAAGAAGGCAGGCCTGTGGCCGTTCAC
GCACTTCAACCGCACGCATGTGGCGCCTCCGCTGATTATCAGCGAGGAAGATCTGCGGCGCGGGCTCTCGATCATCGACG
CGGCGCTGAATGTCGCCGACGGGTACGTGGATTAA

Upstream 100 bases:

>100_bases
GTTCGCGACCGCGACGAGATGACCCAGCAGCGTATTCCGATCGGCGGCGTGGCCGATCATCTCGCCAAAACCCTCAAGGG
CTGCTAGCCGAGGAGCAGAA

Downstream 100 bases:

>100_bases
CCGGTAAAGGCGCCGCTGTAGGCGTTGAGGGCAGGCTGGCCGCCCAGATGGGCATACAGCACGGTTGAGCCGGGCTCGAT
CGTCCCGTTCGTGACCAGGT

Product: aminotransferase

Products: L-Glutamate; Succinic semialdehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTDVQNHIGIDPASAPIEAPAAEAQVRADDRGHVFHSWSAQAMIDPLPIAGGSGATFWDYTGKEYLDFASQLVNLNLGHQ
HPRLVESIQRQAGRLATIAPAFANDVRGELARLVAERAPGTLNKVFFTNGGTEAVEHAVRMARHHTGRRKVLAAYRSYHG
GTTTSMSLTGEPRRWANDPGDNSVVHFFGPYAYRSVFHSTTPEEETQRALEHLEQVINLEGPPTIAAIILESVVGTNGVL
VPPPGYLAGVREICDRYGLVFIADEVMVGFGRFGEWFAINAFDVTPDLITFAKGINSGYVPLGGVVISDAIATSFDHRPY
PGGLTYSGHPLACAVGVETIKVFEDDQILKRVRDLGERVVRPELERWVQAHPSVGEIRGRGLFWAVELVRNKETREPLVP
FNASGEAAAPMNAFAAACKKAGLWPFTHFNRTHVAPPLIISEEDLRRGLSIIDAALNVADGYVD

Sequences:

>Translated_464_residues
MTDVQNHIGIDPASAPIEAPAAEAQVRADDRGHVFHSWSAQAMIDPLPIAGGSGATFWDYTGKEYLDFASQLVNLNLGHQ
HPRLVESIQRQAGRLATIAPAFANDVRGELARLVAERAPGTLNKVFFTNGGTEAVEHAVRMARHHTGRRKVLAAYRSYHG
GTTTSMSLTGEPRRWANDPGDNSVVHFFGPYAYRSVFHSTTPEEETQRALEHLEQVINLEGPPTIAAIILESVVGTNGVL
VPPPGYLAGVREICDRYGLVFIADEVMVGFGRFGEWFAINAFDVTPDLITFAKGINSGYVPLGGVVISDAIATSFDHRPY
PGGLTYSGHPLACAVGVETIKVFEDDQILKRVRDLGERVVRPELERWVQAHPSVGEIRGRGLFWAVELVRNKETREPLVP
FNASGEAAAPMNAFAAACKKAGLWPFTHFNRTHVAPPLIISEEDLRRGLSIIDAALNVADGYVD
>Mature_463_residues
TDVQNHIGIDPASAPIEAPAAEAQVRADDRGHVFHSWSAQAMIDPLPIAGGSGATFWDYTGKEYLDFASQLVNLNLGHQH
PRLVESIQRQAGRLATIAPAFANDVRGELARLVAERAPGTLNKVFFTNGGTEAVEHAVRMARHHTGRRKVLAAYRSYHGG
TTTSMSLTGEPRRWANDPGDNSVVHFFGPYAYRSVFHSTTPEEETQRALEHLEQVINLEGPPTIAAIILESVVGTNGVLV
PPPGYLAGVREICDRYGLVFIADEVMVGFGRFGEWFAINAFDVTPDLITFAKGINSGYVPLGGVVISDAIATSFDHRPYP
GGLTYSGHPLACAVGVETIKVFEDDQILKRVRDLGERVVRPELERWVQAHPSVGEIRGRGLFWAVELVRNKETREPLVPF
NASGEAAAPMNAFAAACKKAGLWPFTHFNRTHVAPPLIISEEDLRRGLSIIDAALNVADGYVD

Specific function: Essential for glycerol catabolism [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=427, Percent_Identity=30.2107728337237, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI24119277, Length=419, Percent_Identity=29.8329355608592, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI13994255, Length=434, Percent_Identity=27.6497695852535, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI37574042, Length=422, Percent_Identity=29.3838862559242, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI226442705, Length=422, Percent_Identity=28.9099526066351, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI226442709, Length=390, Percent_Identity=29.7435897435897, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI284507298, Length=342, Percent_Identity=29.2397660818713, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1789016, Length=414, Percent_Identity=32.6086956521739, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1787560, Length=351, Percent_Identity=36.4672364672365, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI145693181, Length=347, Percent_Identity=29.6829971181556, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789759, Length=346, Percent_Identity=31.5028901734104, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788044, Length=413, Percent_Identity=28.3292978208232, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1786349, Length=330, Percent_Identity=30.3030303030303, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786991, Length=458, Percent_Identity=26.8558951965066, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32564660, Length=440, Percent_Identity=30.6818181818182, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI25144271, Length=421, Percent_Identity=28.2660332541568, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71992977, Length=494, Percent_Identity=26.3157894736842, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25144274, Length=306, Percent_Identity=25.1633986928105, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17541228, Length=367, Percent_Identity=23.9782016348774, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71981843, Length=102, Percent_Identity=40.1960784313725, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=429, Percent_Identity=30.3030303030303, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6324432, Length=371, Percent_Identity=26.6846361185984, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324386, Length=482, Percent_Identity=23.6514522821577, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161085790, Length=435, Percent_Identity=30.5747126436782, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28574759, Length=454, Percent_Identity=29.7356828193833, Blast_Score=187, Evalue=9e-48,
Organism=Drosophila melanogaster, GI21357415, Length=420, Percent_Identity=28.3333333333333, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21356575, Length=362, Percent_Identity=32.0441988950276, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI281366494, Length=377, Percent_Identity=23.342175066313, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667139, Length=377, Percent_Identity=23.342175066313, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667143, Length=377, Percent_Identity=23.342175066313, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.19 [C]

Molecular weight: Translated: 50555; Mature: 50423

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVQNHIGIDPASAPIEAPAAEAQVRADDRGHVFHSWSAQAMIDPLPIAGGSGATFWDY
CCCCCHHCCCCCCCCCCCCCCCCCEECCCCCCCEEECCCCCCCCCCCCCCCCCCCEEECC
TGKEYLDFASQLVNLNLGHQHPRLVESIQRQAGRLATIAPAFANDVRGELARLVAERAPG
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHCCC
TLNKVFFTNGGTEAVEHAVRMARHHTGRRKVLAAYRSYHGGTTTSMSLTGEPRRWANDPG
CCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCC
DNSVVHFFGPYAYRSVFHSTTPEEETQRALEHLEQVINLEGPPTIAAIILESVVGTNGVL
CCCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
VPPPGYLAGVREICDRYGLVFIADEVMVGFGRFGEWFAINAFDVTPDLITFAKGINSGYV
ECCCHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCC
PLGGVVISDAIATSFDHRPYPGGLTYSGHPLACAVGVETIKVFEDDQILKRVRDLGERVV
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHH
RPELERWVQAHPSVGEIRGRGLFWAVELVRNKETREPLVPFNASGEAAAPMNAFAAACKK
HHHHHHHHHCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AGLWPFTHFNRTHVAPPLIISEEDLRRGLSIIDAALNVADGYVD
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDVQNHIGIDPASAPIEAPAAEAQVRADDRGHVFHSWSAQAMIDPLPIAGGSGATFWDY
CCCCHHCCCCCCCCCCCCCCCCCEECCCCCCCEEECCCCCCCCCCCCCCCCCCCEEECC
TGKEYLDFASQLVNLNLGHQHPRLVESIQRQAGRLATIAPAFANDVRGELARLVAERAPG
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHCCC
TLNKVFFTNGGTEAVEHAVRMARHHTGRRKVLAAYRSYHGGTTTSMSLTGEPRRWANDPG
CCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCC
DNSVVHFFGPYAYRSVFHSTTPEEETQRALEHLEQVINLEGPPTIAAIILESVVGTNGVL
CCCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
VPPPGYLAGVREICDRYGLVFIADEVMVGFGRFGEWFAINAFDVTPDLITFAKGINSGYV
ECCCHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCC
PLGGVVISDAIATSFDHRPYPGGLTYSGHPLACAVGVETIKVFEDDQILKRVRDLGERVV
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHH
RPELERWVQAHPSVGEIRGRGLFWAVELVRNKETREPLVPFNASGEAAAPMNAFAAACKK
HHHHHHHHHCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AGLWPFTHFNRTHVAPPLIISEEDLRRGLSIIDAALNVADGYVD
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5'-phosphate lysine-residue [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.3 {4-aminobutanoate}} 1.1 {4-aminobutanoate}} 1.3 {2-oxoglutarate}} 0.7 {2-oxoglutarate}} [C]

Substrates: 4-Aminobutanoate; 2-Oxoglutarate [C]

Specific reaction: 4-Aminobutanoate + 2-Oxoglutarate --> L-Glutamate + Succinic semialdehyde [C]

General reaction: Amino group transfer [C]

Inhibitor: 2-Oxoglutarate; Lysyl reagents; SH-group reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8969498; 8436953 [H]