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The map label for this gene is tig [H]

Identifier: 169834028

GI number: 169834028

Start: 460860

End: 462143

Strand: Direct

Name: tig [H]

Synonym: SPH_0509

Alternate gene names: 169834028

Gene position: 460860-462143 (Clockwise)

Preceding gene: 169833664

Following gene: 169832678

Centisome position: 20.52

GC content: 40.97

Gene sequence:

>1284_bases
ATGTCTGTATCATTTGAAAACAAAGAAACAAACCGTGGTGTCTTGACTTTCACTATCTCTCAAGACCAAATCAAACCAGA
ATTGGACCGTGTCTTCAAGTCAGTGAAGAAATCTCTTAATGTTCCAGGTTTCCGTAAAGGTCACCTTCCACGTCCTATCT
TCGATAAAAAATTTGGTGAAGAATCACTTTACCAAGACGTTATGAACGCTCTTTTGCCAAACGCTTATGAAGCAGCTGTA
AAAGAAGCTGGTCTTGAAGTGGTTGCCCAACCAAAAATTGACGTAACTTCAATGGAAAAAGGTCAAGACTGGGTTATCAC
TGCTGAAGTCGTTACAAAACCTGAAGTAAAATTGGGTGACTACAAAAACCTTGAAGTATCAGTTGATGTAGAAAAAGAAG
TAACTGACGCTGATGTTGAAGAGCGTATCGAACGCGAACGCAACAACCTGGCTGAATTGGTTATCAAGGAAGCTGCTGCT
GAAAACGGCGACACTGTTGTGATCGACTTCGTTGGTTCTATCGACGGTGTTGAATTTGACGGTGGAAAAGGTGAAAACTT
CTCACTTGGACTTGGTTCAGGTCAATTCATCCCTGGTTTCGAAGACCAATTGGTAGGTCACTCAGCTGGCGAAACCGTTG
ATGTTATCGTAACATTCCCAGAAGACTACCAAGCAGAAGACCTTGCAGGTAAAGAAGCTCAATTCGTGACAACTATCCAC
GAAGTAAAAGCTAAAGAAGTTCCAGCTCTTGACGATGAACTTGCAAAAGACATTGATGAAGAAGTTGAAACACTTGCTGA
CTTGAAAGAAAAATACCGCAAAGAATTGGCTGCTGCTAAAGAAGAAGCTTACAAAGATGCAGTTGAAGGTGCAGCAATTG
ATACAGCTGTAGAAAACGCTGAAATCGTAGAACTTCCAGAAGAAATGATCCACGAAGAAGTTCACCGTTCAGTAAATGAA
TTCCTTGGGAACTTGCAACGTCAAGGGATCAACCCTGACATGTACTTCCAAATCACTGGAACTACTCAAGAAGACCTTCA
TAACCAATACCAAGCAGAAGCTGAGTCACGTACTAAGACTAACCTTGTTATCGAAGCAGTTGCCAAAGCTGAAGGATTTG
ATGCTTCAGAAGAAGAAATCCAAAAAGAAGTTGAGCAATTGGCAGCAGACTACAACATGGAAGTTGCACAAGTTCAAAAC
TTGCTTTCAGCTGACATGTTGAAACATGATATCACTATCAAAAAAGCTGTTGAATTGATCACAAGCACAGCAACAGTAAA
ATAA

Upstream 100 bases:

>100_bases
AAAGACTTGTTGTCTATAATTGTCTGCATTAGTCTAGATTTTATTTATAGAAAATGTTATAATAGACTGTATTTAAAAAA
TTTTAAGGAGAAATGACAGA

Downstream 100 bases:

>100_bases
TCTTAATAAACAGAAAACCCACCTGAATTGGTGGGTTTTCTGATGTACTATTTTCCAAAAATCTCTTTGAGGTCTGTGTC
TGTAATCCCAATCATGGCTG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAV
KEAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAQFVTTIH
EVKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNE
FLGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQN
LLSADMLKHDITIKKAVELITSTATVK

Sequences:

>Translated_427_residues
MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAV
KEAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAQFVTTIH
EVKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNE
FLGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQN
LLSADMLKHDITIKKAVELITSTATVK
>Mature_426_residues
SVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVK
EAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAAE
NGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAQFVTTIHE
VKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEF
LGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQNL
LSADMLKHDITIKKAVELITSTATVK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=397, Percent_Identity=31.4861460957179, Blast_Score=176, Evalue=4e-45,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 47312; Mature: 47181

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDKKFGE
CCCCCCCCCCCCCEEEEEECHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCH
ESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGD
HHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
YKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAAENGDTVVIDFVGSIDGVEFD
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEC
GGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAQFVTTIH
CCCCCCEEEECCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCHHCCCCCHHHHHHHH
EVKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEEAYKDAVEGAAIDTAVENA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
EIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKT
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
NLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQNLLSADMLKHDITIKKAVELI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSTATVK
HHHHCCC
>Mature Secondary Structure 
SVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGFRKGHLPRPIFDKKFGE
CCCCCCCCCCCCEEEEEECHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCH
ESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQDWVITAEVVTKPEVKLGD
HHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
YKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAAENGDTVVIDFVGSIDGVEFD
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEC
GGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVTFPEDYQAEDLAGKEAQFVTTIH
CCCCCCEEEECCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCHHCCCCCHHHHHHHH
EVKAKEVPALDDELAKDIDEEVETLADLKEKYRKELAAAKEEAYKDAVEGAAIDTAVENA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
EIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDMYFQITGTTQEDLHNQYQAEAESRTKT
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
NLVIEAVAKAEGFDASEEEIQKEVEQLAADYNMEVAQVQNLLSADMLKHDITIKKAVELI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSTATVK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA