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The map label for this gene is lacG1 [H]

Identifier: 169833379

GI number: 169833379

Start: 536907

End: 538319

Strand: Direct

Name: lacG1 [H]

Synonym: SPH_0589

Alternate gene names: 169833379

Gene position: 536907-538319 (Clockwise)

Preceding gene: 169832896

Following gene: 169833000

Centisome position: 23.91

GC content: 35.03

Gene sequence:

>1413_bases
ATGGAAAATTTACAGGTTAAAGCCTTACCGAAGGAGTTTTTATTAGGAGCTGCTACCGCTGCTTATCAAGTAGAGGGTGC
AACTAGGGTAGATGGCAAAGGAACAAATATGTGGGATGTTTATTTGCAAGAAAACAGTCCGTTCTTACCAGATCCAGCTA
GTGATTTTTATTATCGTTACGAAGAGGATATAGCTTTGGCGGCAGAACATGGTTTGCAGGCTTTGCGTTTATCTATTTCT
TGGGTTCGTATATTTCCTGATATAGATGGGGAGCCTAATATATTAGCTGTTCGTTATTACCATAGAGTCTTTCAGTCTTG
CTTAAAACATAATGTGATTCCGTTTGTTTCTTTACATCATTTTGATTCGCCTCAGAAAATGTTAGAAACAGGGGATTGGT
TGAATAGAGAGAATATTGATCGTTTCATACGATATGCTCGCTTTTGTTTCCAAGAATTTACAGAAGTCAAGCATTGGTTT
ACAATCAATGAACTGATGTCTCTTGCTGCAGGTCAATATATAGGAGGTCAGTTTCCTCCAAATCATCATTTTCAATTATC
TGAAGCAATTCAAGCGAATCATAATATGTTGTTGGCGCATGCTCTTGCAGTCCTCGAATTTCATCAATTAGGGATTGAGG
GAAAGATAGGTTGTATTCATGCTTTAAAGCCAGGCTATCCTATTGATGGGCAAAAAGAAAATATTTTAGCAGCTAAACGG
TATGATGTTTATAATAATAAATTTCTATTAGATGGAACTTTTTTGGGCTACTACAGTGATGACACGCTTTTTTACTTGAA
TCAAATATTGGAAGCTAATAATTCTAGCTTTATTATTGAAGATGGTGATTTAGAAATTATGAAAAGAGCTGCACCTCTTA
ATACGATGTTTGGGATGAATTATTATCGTTCAGAATTTATTCGTGAATACAAAGGTGAAAATAGACAAGAATTTAATTCA
ACAGGAATAAAAGGACAGTCTTCTTTTAAATTAAATGCTCTAGGTGAATTTGTAAAAAAGCCTGGTATTCCGACAACAGA
TTGGGATTGGAATATTTATCCTCAAGGGTTATTTGATATGTTGCTTCGTATCAAAGAAGAATATCCTCAACATCCGGTCA
TTTATTTAACTGAAAATGGTACAGCCCTTAAAGAGGTTAAGCCAGAGGGCGAGAATGATATTATTGATGACAGTAAAAGA
ATCCGTTATATTGAGCAACATTTACACAAAGTTTTAGAGGCTCGAGATAGGGGAGTCAATATTCAAGGCTATTTTATATG
GTCTTTGCAAGATCAATTTTCTTGGGCGAATGGCTACAATAAGCGATATGGTCTTTTCTTTGTTGATTATGAAACACAGA
AGAGATATATTAAGAAAAGTGCTCTTTGGGTAAAAGGATTAAAACGGAATTAA

Upstream 100 bases:

>100_bases
GACAACGATGCTATTGTATGATCAGGTCAAGTTTTTTATTGATGAATATGAACGAATTCGAAAGATTGAAGAACATATTG
GTTTGCAATGAGGATTAGTC

Downstream 100 bases:

>100_bases
GGTTAGCGATTTGACTGATGTTTAATATATTTTAACTATGAGCTTAAATTTTTTACAGGAGGAGTTTTTATGGATAAGTT
AGTCGCTGCCATTGAAAAGC

Product: 6-phospho-beta-galactosidase

Products: NA

Alternate protein names: Beta-D-phosphogalactoside galactohydrolase 1; PGALase 1; P-beta-Gal 1; PBG 1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQENSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSIS
WVRIFPDIDGEPNILAVRYYHRVFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGCIHALKPGYPIDGQKENILAAKR
YDVYNNKFLLDGTFLGYYSDDTLFYLNQILEANNSSFIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNS
TGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKR
IRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN

Sequences:

>Translated_470_residues
MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQENSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSIS
WVRIFPDIDGEPNILAVRYYHRVFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGCIHALKPGYPIDGQKENILAAKR
YDVYNNKFLLDGTFLGYYSDDTLFYLNQILEANNSSFIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNS
TGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKR
IRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN
>Mature_470_residues
MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQENSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSIS
WVRIFPDIDGEPNILAVRYYHRVFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGCIHALKPGYPIDGQKENILAAKR
YDVYNNKFLLDGTFLGYYSDDTLFYLNQILEANNSSFIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNS
TGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKR
IRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN

Specific function: Can Hydrolyze Salicin, Cellobiose, And Probably Arbutin. [C]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=467, Percent_Identity=32.1199143468951, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI32481206, Length=465, Percent_Identity=30.3225806451613, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI110681710, Length=480, Percent_Identity=28.5416666666667, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI28376633, Length=199, Percent_Identity=34.1708542713568, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI24497614, Length=217, Percent_Identity=34.5622119815668, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1789070, Length=489, Percent_Identity=32.1063394683027, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI2367174, Length=489, Percent_Identity=31.0838445807771, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI2367270, Length=486, Percent_Identity=33.3333333333333, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17552856, Length=480, Percent_Identity=32.7083333333333, Blast_Score=200, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17539390, Length=489, Percent_Identity=29.6523517382413, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI21356577, Length=470, Percent_Identity=27.2340425531915, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005928 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.85 [H]

Molecular weight: Translated: 54691; Mature: 54691

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQENSPFLPDPASDFYYRY
CCCCEEEECCHHHHHHHHHHEEEECCCEEECCCCCCEEEEEEECCCCCCCCCCCHHCEEE
EEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHRVFQSCLKHNVIPFVSLHH
CCCHHHHHHHCHHEEEEEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEC
FDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWFTINELMSLAAGQYIGGQFPP
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCCC
NHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGCIHALKPGYPIDGQKENILAAKR
CCCEEHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEE
YDVYNNKFLLDGTFLGYYSDDTLFYLNQILEANNSSFIIEDGDLEIMKRAAPLNTMFGMN
EECCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCHHHHCCH
YYRSEFIREYKGENRQEFNSTGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDM
HHHHHHHHHHCCCCCHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
LLRIKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKRIRYIEQHLHKVLEARDRGVN
HHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
IQGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN
EEEEEEEEECCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQENSPFLPDPASDFYYRY
CCCCEEEECCHHHHHHHHHHEEEECCCEEECCCCCCEEEEEEECCCCCCCCCCCHHCEEE
EEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHRVFQSCLKHNVIPFVSLHH
CCCHHHHHHHCHHEEEEEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEC
FDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWFTINELMSLAAGQYIGGQFPP
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCCC
NHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGCIHALKPGYPIDGQKENILAAKR
CCCEEHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEE
YDVYNNKFLLDGTFLGYYSDDTLFYLNQILEANNSSFIIEDGDLEIMKRAAPLNTMFGMN
EECCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCHHHHCCH
YYRSEFIREYKGENRQEFNSTGIKGQSSFKLNALGEFVKKPGIPTTDWDWNIYPQGLFDM
HHHHHHHHHHCCCCCHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
LLRIKEEYPQHPVIYLTENGTALKEVKPEGENDIIDDSKRIRYIEQHLHKVLEARDRGVN
HHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
IQGYFIWSLQDQFSWANGYNKRYGLFFVDYETQKRYIKKSALWVKGLKRN
EEEEEEEEECCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]