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The map label for this gene is lacF [H]

Identifier: 169832896

GI number: 169832896

Start: 536553

End: 536897

Strand: Direct

Name: lacF [H]

Synonym: SPH_0588

Alternate gene names: 169832896

Gene position: 536553-536897 (Clockwise)

Preceding gene: 169834219

Following gene: 169833379

Centisome position: 23.89

GC content: 36.81

Gene sequence:

>345_bases
ATGAAATCAAAAGAAGAAATAAATATGCTTGGTTTTACAATTGTCGCTTACGCAGGAGATGCAAGGTCAGATTTGATGGA
TGCTTTGGCGTTTGCGAGAGATGGATATTTTGAACAGGCAAGAGAATTGGTTGAGTCTGCAAACGACTCAATAGTGTCTG
CCCATCGAGAACAGACTAATTTATTAGCGGAGGAGGCATCTGGAGATAATTTTGAAGTGAGTTTTATCATGATTCATGGT
CAAGATACTTTGATGACAACGATGCTATTGTATGATCAGGTCAAGTTTTTTATTGATGAATATGAACGAATTCGAAAGAT
TGAAGAACATATTGGTTTGCAATGA

Upstream 100 bases:

>100_bases
GATTTTTAAGATATTTAGGGTCGACTTTCTATTTATATCGTAGCGAAGTCATTTTAATAATGATGTGTAAAAGATGGATC
AAGATTGAGGAGGAAGAAAG

Downstream 100 bases:

>100_bases
GGATTAGTCATGGAAAATTTACAGGTTAAAGCCTTACCGAAGGAGTTTTTATTAGGAGCTGCTACCGCTGCTTATCAAGT
AGAGGGTGCAACTAGGGTAG

Product: lactose-specific phosphotransferase enzyme IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Lac; EIII-Lac; PTS system lactose-specific EIIA component [H]

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELVESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHG
QDTLMTTMLLYDQVKFFIDEYERIRKIEEHIGLQ

Sequences:

>Translated_114_residues
MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELVESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHG
QDTLMTTMLLYDQVKFFIDEYERIRKIEEHIGLQ
>Mature_114_residues
MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELVESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHG
QDTLMTTMLLYDQVKFFIDEYERIRKIEEHIGLQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 13046; Mature: 13046

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELVESANDSIVSAHREQTN
CCCHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYERIRKIEEHIGLQ
HHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELVESANDSIVSAHREQTN
CCCHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYERIRKIEEHIGLQ
HHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3130296; 9066115 [H]