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The map label for this gene is paaG [H]

Identifier: 169832895

GI number: 169832895

Start: 476683

End: 477468

Strand: Direct

Name: paaG [H]

Synonym: SPH_0523

Alternate gene names: 169832895

Gene position: 476683-477468 (Clockwise)

Preceding gene: 169833089

Following gene: 169833599

Centisome position: 21.23

GC content: 43.13

Gene sequence:

>786_bases
ATGGAACACATTATTTATCAACTTGAAGAGGACTTGGCAATCCTTACCTTGAACCGTCCTGAGGTCGCAAATGGTTTTCA
TATTCCCATGTGTGAGGAGATTTTAGAAGCTCTGACTTTGGCAGAAGAAAATCCAGCTGTGCATTTTATCTTAATCAATG
CCAATGGAAAGGTCTTCTCAGTTGGGGGAGATTTGGTAGAGATGAAGCGGGCAGTGGATGAGGATGATATTCCATCATTG
ACAAAAATCGCAGAGTTGGTCAATACGATTTCTTATAAAATCAAGCAAATAGCCAAACCTGTTTTAATGGAAGTTGACGG
TGCTGTTGCAGGTGCCGCAGCGAATATGGCTGTTGCGGCAGATTTCTGTTTGGCGACGGATAAGGCTAAGTTTATCCAAG
CCTTTGTTGGTGTTGGTTTGGCTCCAGATGCAGGGGGAATTCATCTCTTGAGCCGCAGTATCGGTGTGACGCGTGCTGCT
CAATTAGCTATGACAGGAGAGGCTTTAACGGCAGAAAAAGCCCTAGAATGGGGGTTGGTTTACCGCGTTTCTGAAGCTGA
AAAACTTGAAAAGACGAGAGAACAGCTTCTTAAAAAATTAAGACGTGCTTCAAGTAATTCCTATGCTGCCATTAAGAAGT
TGGTTTGGGAGAGCCAATTTAAAGACTGGCAGGGTTACGCTACTTTAGAACTGAACCTACAGAAATCCTTAGCTCAAACA
GAGGATTTCAAAGAAGGAGTGCGAGCTCATTCGGAGAGAAGAAGACCTAAATTTACAGGAAAATAA

Upstream 100 bases:

>100_bases
TCAGCTCAAAATACTGTTTTGAGGTTGCAGATGGAAGCTGACGCGGTTTAAAGAGATTTTCGAAGAGTATAAACTGCTTA
TAAAATAAAAAGGAGCCCTG

Downstream 100 bases:

>100_bases
AAAATACTTGCATCATTCTTTGAATTTTGATATAATTCTTTCTGTCAAATGTTTTGATTGTAAAAGTTTTTTGAAGAAGG
AGGGATATGAAAAATGGACT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEENPAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSL
TKIAELVNTISYKIKQIAKPVLMEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWESQFKDWQGYATLELNLQKSLAQT
EDFKEGVRAHSERRRPKFTGK

Sequences:

>Translated_261_residues
MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEENPAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSL
TKIAELVNTISYKIKQIAKPVLMEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWESQFKDWQGYATLELNLQKSLAQT
EDFKEGVRAHSERRRPKFTGK
>Mature_261_residues
MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEENPAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSL
TKIAELVNTISYKIKQIAKPVLMEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWESQFKDWQGYATLELNLQKSLAQT
EDFKEGVRAHSERRRPKFTGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=29.5546558704453, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI37594471, Length=204, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI37594469, Length=204, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221307494, Length=170, Percent_Identity=27.0588235294118, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=23.5772357723577, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=160, Percent_Identity=27.5, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=160, Percent_Identity=27.5, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI70995211, Length=179, Percent_Identity=29.0502793296089, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI260274832, Length=219, Percent_Identity=22.8310502283105, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI45643119, Length=219, Percent_Identity=22.8310502283105, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI260275230, Length=219, Percent_Identity=22.8310502283105, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=34.6007604562738, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=28.735632183908, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=30.46875, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082183, Length=258, Percent_Identity=25.5813953488372, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=27.1698113207547, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790281, Length=194, Percent_Identity=25.2577319587629, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=26.4822134387352, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=30.2325581395349, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=187, Percent_Identity=28.8770053475936, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=193, Percent_Identity=27.4611398963731, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=234, Percent_Identity=26.0683760683761, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=234, Percent_Identity=26.0683760683761, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=176, Percent_Identity=26.7045454545455, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=221, Percent_Identity=25.7918552036199, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=181, Percent_Identity=27.0718232044199, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=179, Percent_Identity=25.6983240223464, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=200, Percent_Identity=21, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19922422, Length=248, Percent_Identity=25.8064516129032, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=26.3565891472868, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24654903, Length=205, Percent_Identity=23.9024390243902, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920382, Length=176, Percent_Identity=29.5454545454545, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571730, Length=188, Percent_Identity=26.063829787234, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28571729, Length=188, Percent_Identity=26.063829787234, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28723; Mature: 28723

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEENPAVHFILINANGKVFS
CCCHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
VGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVLMEVDGAVAGAAANMAVAA
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAAQLAMTGEALTAEKALEWGLV
HHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHE
YRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWESQFKDWQGYATLELNLQKSLAQT
EEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHH
EDFKEGVRAHSERRRPKFTGK
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEENPAVHFILINANGKVFS
CCCHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
VGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVLMEVDGAVAGAAANMAVAA
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAAQLAMTGEALTAEKALEWGLV
HHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHE
YRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWESQFKDWQGYATLELNLQKSLAQT
EEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHH
EDFKEGVRAHSERRRPKFTGK
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]