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The map label for this gene is potD [H]

Identifier: 169824263

GI number: 169824263

Start: 628011

End: 629036

Strand: Direct

Name: potD [H]

Synonym: FMG_0566

Alternate gene names: 169824263

Gene position: 628011-629036 (Clockwise)

Preceding gene: 169824262

Following gene: 169824264

Centisome position: 34.94

GC content: 30.41

Gene sequence:

>1026_bases
ATGAAGAAAATTTTATTATTTTTATTAACTATATTGGTTTTGACTTCTTGCTCCAAGGATGAATCGAATGTCTTGTATGT
GTACAATTGGGGAGAGTACATTGAACCAGCTTTACTAGATAAGTTTGAAAAAGAAACGGGAATTAAAGTAATCTATGATA
CTTTTGAGCAAAACGAAGATATGTATATGAAAGTAAAAGAAGGAGGCAGCAATTACGATGTTGTTGTTCCTTCTGATTAC
ATGGCTGAAAAAATGATTAAGCAAGGGATGCTTGAAAAAATTGATTATAGTAAAATTCCAAACTTCAAATACATCGATCA
AAGATTTAAAAATTTAGATTATGATCCTAAAAATGAATATACAGTTCCATATATGTGGGGAACTGTTGGAATTCTTTACA
ATAAAAACAAAGTAAAAGAAAAAGTCGACAGCTGGAATATTTTGTGGGATGAAAAGTACAAGGACAACATCATAATGATG
AACTCCACAAGAGATACTCTAGGAGTAGCATTGAAAAGATTAGGATATTCGATGAATTCAAGAAATGAGGCTGAGTTAGA
GAAAGCAAAACAGTCATTAATAAAACAAAAAGATTTGGTACTTGCTTATCTTGTAGATGAAACTAAAACTCAAATGGTCA
ATGAAGAAGCAGATCTCGCAGTAATGTATTCAGGGGATGCAATTGTTTCTAAAGGGGAAAATGAAAATTTGGAATATGTA
ATTCCAAAAGAAGGATCAAATTTGTGGTTTGATACATTGGCTATTTTGAAAAACGCAAAAAATAAGGAAAACGCCGAAAA
ATTTATCAATTTTATGACAAATCCTGAAAATGCAAAATTAAATGCAGAATACATTGGTTATTCTCTTCCATCTACTCAAG
CAAGAAAACTTTTGGATAAAGAAATCCAAGAAGATAAAACAGCTTATCCTGATTTATCAAAGCATAAAAACATGGAAATA
TTCAAGGATCCATCAGATTTTGTAGAAAAATACGATGATATATGGGCTGATATAAAAGCAAATTAA

Upstream 100 bases:

>100_bases
AATCCAGCAATTAATGCTTTAAGCACAATTATGTTTTTGGTAATGGTTATTCTATTGTTGATTCTTCACAAGAGAACAAA
TCTATTGGAGGAGTTATAAT

Downstream 100 bases:

>100_bases
TTTGAAAAAGTGCAAGAGATTTTCTTTTGTACTTTTTTAGTTTGAGATTGAATAATAGACGAAATTTTCATATAATATGA
TTAGTTAAGAAATTGGAGGA

Product: spermidine/putrescine ABC transporter ATP-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKKILLFLLTILVLTSCSKDESNVLYVYNWGEYIEPALLDKFEKETGIKVIYDTFEQNEDMYMKVKEGGSNYDVVVPSDY
MAEKMIKQGMLEKIDYSKIPNFKYIDQRFKNLDYDPKNEYTVPYMWGTVGILYNKNKVKEKVDSWNILWDEKYKDNIIMM
NSTRDTLGVALKRLGYSMNSRNEAELEKAKQSLIKQKDLVLAYLVDETKTQMVNEEADLAVMYSGDAIVSKGENENLEYV
IPKEGSNLWFDTLAILKNAKNKENAEKFINFMTNPENAKLNAEYIGYSLPSTQARKLLDKEIQEDKTAYPDLSKHKNMEI
FKDPSDFVEKYDDIWADIKAN

Sequences:

>Translated_341_residues
MKKILLFLLTILVLTSCSKDESNVLYVYNWGEYIEPALLDKFEKETGIKVIYDTFEQNEDMYMKVKEGGSNYDVVVPSDY
MAEKMIKQGMLEKIDYSKIPNFKYIDQRFKNLDYDPKNEYTVPYMWGTVGILYNKNKVKEKVDSWNILWDEKYKDNIIMM
NSTRDTLGVALKRLGYSMNSRNEAELEKAKQSLIKQKDLVLAYLVDETKTQMVNEEADLAVMYSGDAIVSKGENENLEYV
IPKEGSNLWFDTLAILKNAKNKENAEKFINFMTNPENAKLNAEYIGYSLPSTQARKLLDKEIQEDKTAYPDLSKHKNMEI
FKDPSDFVEKYDDIWADIKAN
>Mature_341_residues
MKKILLFLLTILVLTSCSKDESNVLYVYNWGEYIEPALLDKFEKETGIKVIYDTFEQNEDMYMKVKEGGSNYDVVVPSDY
MAEKMIKQGMLEKIDYSKIPNFKYIDQRFKNLDYDPKNEYTVPYMWGTVGILYNKNKVKEKVDSWNILWDEKYKDNIIMM
NSTRDTLGVALKRLGYSMNSRNEAELEKAKQSLIKQKDLVLAYLVDETKTQMVNEEADLAVMYSGDAIVSKGENENLEYV
IPKEGSNLWFDTLAILKNAKNKENAEKFINFMTNPENAKLNAEYIGYSLPSTQARKLLDKEIQEDKTAYPDLSKHKNMEI
FKDPSDFVEKYDDIWADIKAN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=330, Percent_Identity=42.4242424242424, Blast_Score=260, Evalue=9e-71,
Organism=Escherichia coli, GI1787078, Length=323, Percent_Identity=30.6501547987616, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1787711, Length=324, Percent_Identity=25.9259259259259, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39723; Mature: 39723

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLFLLTILVLTSCSKDESNVLYVYNWGEYIEPALLDKFEKETGIKVIYDTFEQNED
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHCCHHHHHHHHHHCCCEEEEECHHCCCC
MYMKVKEGGSNYDVVVPSDYMAEKMIKQGMLEKIDYSKIPNFKYIDQRFKNLDYDPKNEY
EEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHCCCCCCCCCE
TVPYMWGTVGILYNKNKVKEKVDSWNILWDEKYKDNIIMMNSTRDTLGVALKRLGYSMNS
ECEEEECCEEEEECCHHHHHHHCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCCC
RNEAELEKAKQSLIKQKDLVLAYLVDETKTQMVNEEADLAVMYSGDAIVSKGENENLEYV
CCHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCCEEE
IPKEGSNLWFDTLAILKNAKNKENAEKFINFMTNPENAKLNAEYIGYSLPSTQARKLLDK
ECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHHEECCCCCCHHHHHHHHH
EIQEDKTAYPDLSKHKNMEIFKDPSDFVEKYDDIWADIKAN
HHHHHCCCCCCCHHCCCCEEECCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKILLFLLTILVLTSCSKDESNVLYVYNWGEYIEPALLDKFEKETGIKVIYDTFEQNED
CHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHCCHHHHHHHHHHCCCEEEEECHHCCCC
MYMKVKEGGSNYDVVVPSDYMAEKMIKQGMLEKIDYSKIPNFKYIDQRFKNLDYDPKNEY
EEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHCCCCCCCCCE
TVPYMWGTVGILYNKNKVKEKVDSWNILWDEKYKDNIIMMNSTRDTLGVALKRLGYSMNS
ECEEEECCEEEEECCHHHHHHHCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCCC
RNEAELEKAKQSLIKQKDLVLAYLVDETKTQMVNEEADLAVMYSGDAIVSKGENENLEYV
CCHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCCEEE
IPKEGSNLWFDTLAILKNAKNKENAEKFINFMTNPENAKLNAEYIGYSLPSTQARKLLDK
ECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHHEECCCCCCHHHHHHHHH
EIQEDKTAYPDLSKHKNMEIFKDPSDFVEKYDDIWADIKAN
HHHHHCCCCCCCHHCCCCEEECCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]