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The map label for this gene is metG [H]

Identifier: 169824241

GI number: 169824241

Start: 601508

End: 603508

Strand: Direct

Name: metG [H]

Synonym: FMG_0544

Alternate gene names: 169824241

Gene position: 601508-603508 (Clockwise)

Preceding gene: 169824240

Following gene: 169824242

Centisome position: 33.46

GC content: 32.53

Gene sequence:

>2001_bases
ATGTCAGAAGAAAAGAAATCATATTATATTACTACGCCGATTTATTATCCAAGTAGTAATTTACATTTAGGTCATACTTA
TACGACAATTATTGCAGATACTTTGAAAAAATTCAAAAAAATTCAAGGATATGATGTGTTTTTGACTACAGGTACTGATG
AGCATGGTCAAAAATTATACGAAAAAGCAAAAGAAAAAAACATGGGAGATAATCCACTAAATTATATCGATCCAATTGTA
CAAAGCGCTAAGGATTTGTGGAAGAAGCTTGACATTGACATAGATAGTTTTGTTAGAAGTACAGACAAGGTTCACGAAAA
AAATGTTCAAGAAATTTTCCAAAAATTATACGATAAGGGAGATATCTACAAATCTACTTACAAAGGACATTATTGTGTTG
ATTGTGAAGCTTTTTGGACAGAATCACAACTTAAGGATGGAAAGTGTCCAGATTGTGGAAGAGATGTTCACTATCATGAA
GAAGAATCATACTTTTTTAGATTATCAAAATACAAAGATAGAATTTTACAATTATACAAAGATAATCCGGACTTCTTAAA
ACCGGAATCTCGTAAGAACGAAATGGTGAACAACTTTTTAAGTGGTGAATTGGAAGATTTATCTGTATCTCGTACAAGTT
TTGACTGGGGAGTTAAGGTTCCGTTTGATGATAAACATATAATCTACGTATGGATTGATGCGCTTAGCTGCTATTTGACA
GGAATTGGATATGGACAAGACGAAGAATTATTTAATAAATTCTGGCCATGCGATATTCACTTGGTTGGAAAAGAAATTGT
AAGATTCCACACAATTATTTGGCCAGCTTTGTTGATGGCGTTGGATTTGCCATTGCCAAAGAGAGTTTTTGCTCACGGAT
GGATTTTGTTCAATGAAGATAAGATGAGTAAATCAAAGGGAAATGTATATTATCCAGAACCAATCATTGATTTATACGGT
GTGGATGCTTTGAAATACTATATTTTAAGAGATTTTACTTTTGGTAATGATGGTAATTTTACTACAGAAAGATTTATGAA
CAGGTTCAACTCAGATTTGGTAAATGATTTAGGAAATCTTGTGAGTAGATCAGTGTCGATGGTTGAAAAATATTTTGATG
GTGTTATTCCACAACCAAATGACAACAACGATATTGACAAGAGTTTGATATCTGTAGTTGAAGGTTGTCAAGAAAAAGTT
GAAGAGTTATTAGAAGAGCTAGATTTTTCAAATGCGCTTGAAGAAATTTGGAAAGTTGTTAGAAGAGCCAACAAATACAT
CGATGAAACTACTCCTTGGATTTTGATTAAAGAAGATCCAGAAAGATTGAAGACTGTTTTGTATAATTTGATGGATGCGA
TAAGAGTTATCTGTATTTTAACATCTCCATTTATCACTGAAACTAGCAACAAAATAAAAGAACAAATCGGTCTTTCTGAA
ATAAAATGGGAAGATGGAAAAGTGTTTGGTAAAACTGTTGTTGGAACTAGGGTTCAAAGAGGAGAAAACTTGTTCAATAG
ATTGGATATCAAGAAAGAAACTGAAAGATTGAATGAGGTTAACGATAAATTAATCGAAGAAAGAACTGGTAAAAAAGTTT
CTGAATTAAGAGACGATAACGATGAAGAAGCTGCTAAAAAATCCAAGAAAAAATCTAAAAAAGCTAAGACAAAAGAAGAA
ATCACTATAGATGATTTTGACAAGCTTGATTTGAGGGTTGGACAAATCATGGAAGCTAAACAACATCCAGATGCCGATAA
ACTTTTGGTTTTCAAGGTTAAAATCGGAGAAGAAACAAGACAAATAGTTTCTGGTATTAAAAATTGGTATGAACCAAATG
ATTTAATAGGTAAGAAGGTTATTGTCGTGTACAATTTGAAGCCTGTTAAATTAAGAGGGGTAGAATCTCAAGGTATGCTT
CTTGCGGCAAGTAAGGGAGATAACTTGACAATGGCAAGCTTACTTGATTCAGAATTTGAAGACGGAGCGACTGTATCTTA
A

Upstream 100 bases:

>100_bases
CTAAAATTAAGGTGGTAACGCGGATTTTCGCCCTTATATGATGTATGTCATATAAGGGCTTTTTTATTTTATTGTAGTTT
ATTTATTATGGAGGGAAATT

Downstream 100 bases:

>100_bases
TGGGAGATTATGTAATATACGATACGCATTGCCACTTGACAGATGAAAAATTTGATTTTGATAGGGATAAAATAATCGAA
AATCTTAAATCAAATTCTGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 666; Mature: 665

Protein sequence:

>666_residues
MSEEKKSYYITTPIYYPSSNLHLGHTYTTIIADTLKKFKKIQGYDVFLTTGTDEHGQKLYEKAKEKNMGDNPLNYIDPIV
QSAKDLWKKLDIDIDSFVRSTDKVHEKNVQEIFQKLYDKGDIYKSTYKGHYCVDCEAFWTESQLKDGKCPDCGRDVHYHE
EESYFFRLSKYKDRILQLYKDNPDFLKPESRKNEMVNNFLSGELEDLSVSRTSFDWGVKVPFDDKHIIYVWIDALSCYLT
GIGYGQDEELFNKFWPCDIHLVGKEIVRFHTIIWPALLMALDLPLPKRVFAHGWILFNEDKMSKSKGNVYYPEPIIDLYG
VDALKYYILRDFTFGNDGNFTTERFMNRFNSDLVNDLGNLVSRSVSMVEKYFDGVIPQPNDNNDIDKSLISVVEGCQEKV
EELLEELDFSNALEEIWKVVRRANKYIDETTPWILIKEDPERLKTVLYNLMDAIRVICILTSPFITETSNKIKEQIGLSE
IKWEDGKVFGKTVVGTRVQRGENLFNRLDIKKETERLNEVNDKLIEERTGKKVSELRDDNDEEAAKKSKKKSKKAKTKEE
ITIDDFDKLDLRVGQIMEAKQHPDADKLLVFKVKIGEETRQIVSGIKNWYEPNDLIGKKVIVVYNLKPVKLRGVESQGML
LAASKGDNLTMASLLDSEFEDGATVS

Sequences:

>Translated_666_residues
MSEEKKSYYITTPIYYPSSNLHLGHTYTTIIADTLKKFKKIQGYDVFLTTGTDEHGQKLYEKAKEKNMGDNPLNYIDPIV
QSAKDLWKKLDIDIDSFVRSTDKVHEKNVQEIFQKLYDKGDIYKSTYKGHYCVDCEAFWTESQLKDGKCPDCGRDVHYHE
EESYFFRLSKYKDRILQLYKDNPDFLKPESRKNEMVNNFLSGELEDLSVSRTSFDWGVKVPFDDKHIIYVWIDALSCYLT
GIGYGQDEELFNKFWPCDIHLVGKEIVRFHTIIWPALLMALDLPLPKRVFAHGWILFNEDKMSKSKGNVYYPEPIIDLYG
VDALKYYILRDFTFGNDGNFTTERFMNRFNSDLVNDLGNLVSRSVSMVEKYFDGVIPQPNDNNDIDKSLISVVEGCQEKV
EELLEELDFSNALEEIWKVVRRANKYIDETTPWILIKEDPERLKTVLYNLMDAIRVICILTSPFITETSNKIKEQIGLSE
IKWEDGKVFGKTVVGTRVQRGENLFNRLDIKKETERLNEVNDKLIEERTGKKVSELRDDNDEEAAKKSKKKSKKAKTKEE
ITIDDFDKLDLRVGQIMEAKQHPDADKLLVFKVKIGEETRQIVSGIKNWYEPNDLIGKKVIVVYNLKPVKLRGVESQGML
LAASKGDNLTMASLLDSEFEDGATVS
>Mature_665_residues
SEEKKSYYITTPIYYPSSNLHLGHTYTTIIADTLKKFKKIQGYDVFLTTGTDEHGQKLYEKAKEKNMGDNPLNYIDPIVQ
SAKDLWKKLDIDIDSFVRSTDKVHEKNVQEIFQKLYDKGDIYKSTYKGHYCVDCEAFWTESQLKDGKCPDCGRDVHYHEE
ESYFFRLSKYKDRILQLYKDNPDFLKPESRKNEMVNNFLSGELEDLSVSRTSFDWGVKVPFDDKHIIYVWIDALSCYLTG
IGYGQDEELFNKFWPCDIHLVGKEIVRFHTIIWPALLMALDLPLPKRVFAHGWILFNEDKMSKSKGNVYYPEPIIDLYGV
DALKYYILRDFTFGNDGNFTTERFMNRFNSDLVNDLGNLVSRSVSMVEKYFDGVIPQPNDNNDIDKSLISVVEGCQEKVE
ELLEELDFSNALEEIWKVVRRANKYIDETTPWILIKEDPERLKTVLYNLMDAIRVICILTSPFITETSNKIKEQIGLSEI
KWEDGKVFGKTVVGTRVQRGENLFNRLDIKKETERLNEVNDKLIEERTGKKVSELRDDNDEEAAKKSKKKSKKAKTKEEI
TIDDFDKLDLRVGQIMEAKQHPDADKLLVFKVKIGEETRQIVSGIKNWYEPNDLIGKKVIVVYNLKPVKLRGVESQGMLL
AASKGDNLTMASLLDSEFEDGATVS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=512, Percent_Identity=31.640625, Blast_Score=275, Evalue=9e-74,
Organism=Homo sapiens, GI14043022, Length=644, Percent_Identity=23.7577639751553, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI4507947, Length=138, Percent_Identity=38.4057971014493, Blast_Score=83, Evalue=1e-15,
Organism=Homo sapiens, GI215490011, Length=86, Percent_Identity=44.1860465116279, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI215490009, Length=86, Percent_Identity=44.1860465116279, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI45006986, Length=86, Percent_Identity=44.1860465116279, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788432, Length=688, Percent_Identity=26.7441860465116, Blast_Score=249, Evalue=5e-67,
Organism=Escherichia coli, GI1789455, Length=89, Percent_Identity=41.5730337078652, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786861, Length=176, Percent_Identity=26.7045454545455, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI212645096, Length=493, Percent_Identity=32.4543610547667, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI71992781, Length=381, Percent_Identity=33.3333333333333, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17540794, Length=596, Percent_Identity=24.8322147651007, Blast_Score=164, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321610, Length=526, Percent_Identity=30.2281368821293, Blast_Score=248, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6321704, Length=565, Percent_Identity=22.8318584070796, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321333, Length=83, Percent_Identity=39.7590361445783, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21358483, Length=554, Percent_Identity=30.8664259927798, Blast_Score=270, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24655311, Length=572, Percent_Identity=22.3776223776224, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24651924, Length=81, Percent_Identity=44.4444444444444, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357223, Length=82, Percent_Identity=42.6829268292683, Blast_Score=74, Evalue=5e-13,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 77131; Mature: 76999

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKKSYYITTPIYYPSSNLHLGHTYTTIIADTLKKFKKIQGYDVFLTTGTDEHGQKLY
CCCCCCCEEEEEEEEECCCCEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
EKAKEKNMGDNPLNYIDPIVQSAKDLWKKLDIDIDSFVRSTDKVHEKNVQEIFQKLYDKG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DIYKSTYKGHYCVDCEAFWTESQLKDGKCPDCGRDVHYHEEESYFFRLSKYKDRILQLYK
CCCHHHCCCCEEEECHHHHCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
DNPDFLKPESRKNEMVNNFLSGELEDLSVSRTSFDWGVKVPFDDKHIIYVWIDALSCYLT
CCCCCCCCCHHHHHHHHHHHCCCHHHCEEECCCCCCCEECCCCCCEEEEEEHHHHHHHHH
GIGYGQDEELFNKFWPCDIHLVGKEIVRFHTIIWPALLMALDLPLPKRVFAHGWILFNED
HCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCC
KMSKSKGNVYYPEPIIDLYGVDALKYYILRDFTFGNDGNFTTERFMNRFNSDLVNDLGNL
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VSRSVSMVEKYFDGVIPQPNDNNDIDKSLISVVEGCQEKVEELLEELDFSNALEEIWKVV
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RRANKYIDETTPWILIKEDPERLKTVLYNLMDAIRVICILTSPFITETSNKIKEQIGLSE
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
IKWEDGKVFGKTVVGTRVQRGENLFNRLDIKKETERLNEVNDKLIEERTGKKVSELRDDN
EECCCCCEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
DEEAAKKSKKKSKKAKTKEEITIDDFDKLDLRVGQIMEAKQHPDADKLLVFKVKIGEETR
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHH
QIVSGIKNWYEPNDLIGKKVIVVYNLKPVKLRGVESQGMLLAASKGDNLTMASLLDSEFE
HHHHHHHHHCCCCHHCCCEEEEEEECCCEEEECCCCCCEEEEECCCCCCHHHHHHHHHCC
DGATVS
CCCCCC
>Mature Secondary Structure 
SEEKKSYYITTPIYYPSSNLHLGHTYTTIIADTLKKFKKIQGYDVFLTTGTDEHGQKLY
CCCCCCEEEEEEEEECCCCEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
EKAKEKNMGDNPLNYIDPIVQSAKDLWKKLDIDIDSFVRSTDKVHEKNVQEIFQKLYDKG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DIYKSTYKGHYCVDCEAFWTESQLKDGKCPDCGRDVHYHEEESYFFRLSKYKDRILQLYK
CCCHHHCCCCEEEECHHHHCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
DNPDFLKPESRKNEMVNNFLSGELEDLSVSRTSFDWGVKVPFDDKHIIYVWIDALSCYLT
CCCCCCCCCHHHHHHHHHHHCCCHHHCEEECCCCCCCEECCCCCCEEEEEEHHHHHHHHH
GIGYGQDEELFNKFWPCDIHLVGKEIVRFHTIIWPALLMALDLPLPKRVFAHGWILFNED
HCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCC
KMSKSKGNVYYPEPIIDLYGVDALKYYILRDFTFGNDGNFTTERFMNRFNSDLVNDLGNL
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VSRSVSMVEKYFDGVIPQPNDNNDIDKSLISVVEGCQEKVEELLEELDFSNALEEIWKVV
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RRANKYIDETTPWILIKEDPERLKTVLYNLMDAIRVICILTSPFITETSNKIKEQIGLSE
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
IKWEDGKVFGKTVVGTRVQRGENLFNRLDIKKETERLNEVNDKLIEERTGKKVSELRDDN
EECCCCCEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
DEEAAKKSKKKSKKAKTKEEITIDDFDKLDLRVGQIMEAKQHPDADKLLVFKVKIGEETR
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHH
QIVSGIKNWYEPNDLIGKKVIVVYNLKPVKLRGVESQGMLLAASKGDNLTMASLLDSEFE
HHHHHHHHHCCCCHHCCCEEEEEEECCCEEEECCCCCCEEEEECCCCCCHHHHHHHHHCC
DGATVS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]