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The map label for this gene is trmD

Identifier: 169824226

GI number: 169824226

Start: 592403

End: 593104

Strand: Direct

Name: trmD

Synonym: FMG_0529

Alternate gene names: 169824226

Gene position: 592403-593104 (Clockwise)

Preceding gene: 169824225

Following gene: 169824227

Centisome position: 32.96

GC content: 33.19

Gene sequence:

>702_bases
ATGAAATTTATAATTCTCACATTATTTCCAGAAAGTTTTGATTACTTAAAATCATACGGCGTCATAGGAAAAGCAATCCA
AAACAATCTCATTGAACTTGAAGTGGTTAATATTAGAGATTACACTAAAAATAAACACAAAAAAGTAGACGATGAGATTT
ACGGTGGCGGAGCTGGTATGCTTATGACTTGCCAACCAATTTATGACTGCTTGGAAGATGTGAACAAAAACCAATCAAAG
GTTGTGTTTATGTCTCCTCAAGGGAAAGTTCTTAATCAACAAAAGTGTATCGAACTTTCAAAAGAAAAAGAAATAGTTAT
TCTTTGTGGCCATTACGAAGGTGTTGACAGTAGAATCATCAATCATTATTGTGATGAGGAGATTTCTATTGGAGATTATG
TGATGACTGGTGGAGAATTAGGAGCTATGGTTCTTATAGATTGTGTTAGCCGAATGATTAACGATGTTTTGGGAAATGAT
GAAAGTTATAAAACAGATTCGCACTACAATTTATTGCTACAGGAAGATTCCTACACAAGACCCAGAGTATTTAACGGATA
TGAAGTTCCTGAAGTTTTATTATCAGGAAATCACGAAAAAATCGAACAGTGGCGAGAAAAATCAAGACTAAACAACACTA
AACTTAAAAGAGAAGATATATATCAGAAATATCTGAAAGAAAAAAACCAAGGAGGATCATAA

Upstream 100 bases:

>100_bases
ATGATGAAAAAGAATTTATGATACCTGCTGTAAAAGAGTTCATTAAAAAAATAGATTTAGAAAATGGATTAATCGATGTA
GTTATAATTGAGGGTATGTG

Downstream 100 bases:

>100_bases
TGGATATTATAAAAACATTAGAAGATGAACAATTAAGAGAAAATAAGTTCGACTTTCATGTTGGAGATACTGTAAAAGTA
GACTACCTTATTAAAGAAGG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKFIILTLFPESFDYLKSYGVIGKAIQNNLIELEVVNIRDYTKNKHKKVDDEIYGGGAGMLMTCQPIYDCLEDVNKNQSK
VVFMSPQGKVLNQQKCIELSKEKEIVILCGHYEGVDSRIINHYCDEEISIGDYVMTGGELGAMVLIDCVSRMINDVLGND
ESYKTDSHYNLLLQEDSYTRPRVFNGYEVPEVLLSGNHEKIEQWREKSRLNNTKLKREDIYQKYLKEKNQGGS

Sequences:

>Translated_233_residues
MKFIILTLFPESFDYLKSYGVIGKAIQNNLIELEVVNIRDYTKNKHKKVDDEIYGGGAGMLMTCQPIYDCLEDVNKNQSK
VVFMSPQGKVLNQQKCIELSKEKEIVILCGHYEGVDSRIINHYCDEEISIGDYVMTGGELGAMVLIDCVSRMINDVLGND
ESYKTDSHYNLLLQEDSYTRPRVFNGYEVPEVLLSGNHEKIEQWREKSRLNNTKLKREDIYQKYLKEKNQGGS
>Mature_233_residues
MKFIILTLFPESFDYLKSYGVIGKAIQNNLIELEVVNIRDYTKNKHKKVDDEIYGGGAGMLMTCQPIYDCLEDVNKNQSK
VVFMSPQGKVLNQQKCIELSKEKEIVILCGHYEGVDSRIINHYCDEEISIGDYVMTGGELGAMVLIDCVSRMINDVLGND
ESYKTDSHYNLLLQEDSYTRPRVFNGYEVPEVLLSGNHEKIEQWREKSRLNNTKLKREDIYQKYLKEKNQGGS

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=234, Percent_Identity=44.4444444444444, Blast_Score=214, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_FINM2 (B0S047)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691837.1
- ProteinModelPortal:   B0S047
- SMR:   B0S047
- GeneID:   6019223
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0529
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   CLSK2541981
- BioCyc:   FMAG334413:FMG_0529-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26798; Mature: 26798

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIILTLFPESFDYLKSYGVIGKAIQNNLIELEVVNIRDYTKNKHKKVDDEIYGGGAGM
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECHHHCCCCCCCCCHHHCCCCCCE
LMTCQPIYDCLEDVNKNQSKVVFMSPQGKVLNQQKCIELSKEKEIVILCGHYEGVDSRII
EEECHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHH
NHYCDEEISIGDYVMTGGELGAMVLIDCVSRMINDVLGNDESYKTDSHYNLLLQEDSYTR
HHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
PRVFNGYEVPEVLLSGNHEKIEQWREKSRLNNTKLKREDIYQKYLKEKNQGGS
CEEECCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKFIILTLFPESFDYLKSYGVIGKAIQNNLIELEVVNIRDYTKNKHKKVDDEIYGGGAGM
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECHHHCCCCCCCCCHHHCCCCCCE
LMTCQPIYDCLEDVNKNQSKVVFMSPQGKVLNQQKCIELSKEKEIVILCGHYEGVDSRII
EEECHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHH
NHYCDEEISIGDYVMTGGELGAMVLIDCVSRMINDVLGNDESYKTDSHYNLLLQEDSYTR
HHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
PRVFNGYEVPEVLLSGNHEKIEQWREKSRLNNTKLKREDIYQKYLKEKNQGGS
CEEECCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA