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The map label for this gene is yclM [H]

Identifier: 169824014

GI number: 169824014

Start: 369724

End: 371034

Strand: Direct

Name: yclM [H]

Synonym: FMG_0317

Alternate gene names: 169824014

Gene position: 369724-371034 (Clockwise)

Preceding gene: 169824013

Following gene: 169824015

Centisome position: 20.57

GC content: 35.77

Gene sequence:

>1311_bases
ATGAAAGTTGTAAAATTTGGTGGCAGTTCACTGGCTGATGCTAATCAATTGAAAAAAGTTAAGGATATTATCATGAATGA
TGATGCACGCAAGTTCATCGTTGTTTCTGCGCCTGGAAAAGGCGTGAATAATAAGCACAAGGTGACAGATTTATTGGCGA
TGTGTCACGAATTGAGTGCGCATGATTTGAATTTTAATGAGGTTTTCGAGATCATTGAGAATATTTACAAAAATATTGTC
GATGATCTTGTTTTGAATATTGACATTGATAAAATTCTTTCTGATGTAAAAAAAGAAATTACAAATGGCGCAAGTTATGA
TTTTGTTGTGAGTCGTGGTGAGTTTATGAGTGCAAAAGTGCTTGCGGCGTATTTGGATTATGATTTCGTGGATGCGAAGG
ATTTGATTGTATTCGATGATGGCGAGTTGGATATTGTAAAATCACATGAAAATATCAAAAATATTTTGACGAAACACGAC
AGAGCTGTGGTGCCGGGATTTTACGGCGTTGACGAATATAATAATGTACACACTTTTTCTCGCGGGGGTTCTGATGTTAC
AGGATCAGTCATTGCGGCGAGCTTGGACTGTGAGATGTATGAAAATTTCACTGACGTGTCAGGATTTTTGGTTGCAGATC
CAAGAATTGTTAAAAATCCTAAACCGATGGGGACAATTACGTATCATGAACTTCGTGAGCTTTCATATATGGGTGCAAAA
GTTTTGCACGAAGAGGCGATATTTCCACTTAGAGACAAGAACATTCCTATAAATATCAAAAATACAAATGCTCCAGATGA
AGCAGGAACGTTGATTTTGTCGAAGTGCGATGTGAAAAACAAAAATATTGTGACTGGAATTTCTGGAAAGAAAGATTTCA
CTGTTATAAATCTTGAAAAAGTGAATATGAATACGGAGAAGGATTTTTTCAGAAAATTGACGACGGTTTTTGAATCCAAC
GATATTTTGATTGAACACATGCCATCGAGTATTGATTCTGTTTCTGTTTTGGTTTCTGATTCATCCATCACACCGAAATT
AAATAAGGTTCTTGAGGAATTGAAAATTTATTTGGATGTAGATAAGATTTCTTGGGAAAGAGATATCTCGCTAATCGCAG
TGGTTGGTCGAGGTATGATTAATGAAAAAGGAGTATCGTCGAGTACTTTTACAGCTCTTGCAAAAGAAGGCGTAAATATC
AAGATGATCAGCCAAGGTTCCAGCGAAATCAATATTATTATTGGCGTTGAAACAAAAGATTTTGAAAAGGCAATTCGTTC
TATATATAAAGAATTTTACAAGGAGGACTAA

Upstream 100 bases:

>100_bases
AATTTTTGGGTTATTTACTTTGTTCTGATAAATTTACGAGAAAAAATTCTCACGTAGATATACAATTATTATTTAATAGA
TCTAACAAGAAGGGGAAATC

Downstream 100 bases:

>100_bases
ATGAAGGTTAATGTAGCGGTTGTAGGTGCAACTGGACTTGTTGGAAGTATGATGTTAAAAGTTTTGAGTGAGAAAAATTT
TCCAATCGATAAATTATACT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKVVKFGGSSLADANQLKKVKDIIMNDDARKFIVVSAPGKGVNNKHKVTDLLAMCHELSAHDLNFNEVFEIIENIYKNIV
DDLVLNIDIDKILSDVKKEITNGASYDFVVSRGEFMSAKVLAAYLDYDFVDAKDLIVFDDGELDIVKSHENIKNILTKHD
RAVVPGFYGVDEYNNVHTFSRGGSDVTGSVIAASLDCEMYENFTDVSGFLVADPRIVKNPKPMGTITYHELRELSYMGAK
VLHEEAIFPLRDKNIPINIKNTNAPDEAGTLILSKCDVKNKNIVTGISGKKDFTVINLEKVNMNTEKDFFRKLTTVFESN
DILIEHMPSSIDSVSVLVSDSSITPKLNKVLEELKIYLDVDKISWERDISLIAVVGRGMINEKGVSSSTFTALAKEGVNI
KMISQGSSEINIIIGVETKDFEKAIRSIYKEFYKED

Sequences:

>Translated_436_residues
MKVVKFGGSSLADANQLKKVKDIIMNDDARKFIVVSAPGKGVNNKHKVTDLLAMCHELSAHDLNFNEVFEIIENIYKNIV
DDLVLNIDIDKILSDVKKEITNGASYDFVVSRGEFMSAKVLAAYLDYDFVDAKDLIVFDDGELDIVKSHENIKNILTKHD
RAVVPGFYGVDEYNNVHTFSRGGSDVTGSVIAASLDCEMYENFTDVSGFLVADPRIVKNPKPMGTITYHELRELSYMGAK
VLHEEAIFPLRDKNIPINIKNTNAPDEAGTLILSKCDVKNKNIVTGISGKKDFTVINLEKVNMNTEKDFFRKLTTVFESN
DILIEHMPSSIDSVSVLVSDSSITPKLNKVLEELKIYLDVDKISWERDISLIAVVGRGMINEKGVSSSTFTALAKEGVNI
KMISQGSSEINIIIGVETKDFEKAIRSIYKEFYKED
>Mature_436_residues
MKVVKFGGSSLADANQLKKVKDIIMNDDARKFIVVSAPGKGVNNKHKVTDLLAMCHELSAHDLNFNEVFEIIENIYKNIV
DDLVLNIDIDKILSDVKKEITNGASYDFVVSRGEFMSAKVLAAYLDYDFVDAKDLIVFDDGELDIVKSHENIKNILTKHD
RAVVPGFYGVDEYNNVHTFSRGGSDVTGSVIAASLDCEMYENFTDVSGFLVADPRIVKNPKPMGTITYHELRELSYMGAK
VLHEEAIFPLRDKNIPINIKNTNAPDEAGTLILSKCDVKNKNIVTGISGKKDFTVINLEKVNMNTEKDFFRKLTTVFESN
DILIEHMPSSIDSVSVLVSDSSITPKLNKVLEELKIYLDVDKISWERDISLIAVVGRGMINEKGVSSSTFTALAKEGVNI
KMISQGSSEINIIIGVETKDFEKAIRSIYKEFYKED

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=479, Percent_Identity=28.8100208768267, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790455, Length=451, Percent_Identity=25.9423503325942, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790376, Length=469, Percent_Identity=22.6012793176972, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320893, Length=496, Percent_Identity=25.8064516129032, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 48628; Mature: 48628

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVKFGGSSLADANQLKKVKDIIMNDDARKFIVVSAPGKGVNNKHKVTDLLAMCHELSA
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCC
HDLNFNEVFEIIENIYKNIVDDLVLNIDIDKILSDVKKEITNGASYDFVVSRGEFMSAKV
CCCCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
LAAYLDYDFVDAKDLIVFDDGELDIVKSHENIKNILTKHDRAVVPGFYGVDEYNNVHTFS
HHHHHCCCEECCCEEEEEECCCEEEEECHHHHHHHHHCCCCEECCCCCCCCCCCCEEEEC
RGGSDVTGSVIAASLDCEMYENFTDVSGFLVADPRIVKNPKPMGTITYHELRELSYMGAK
CCCCCCCCCEEEEEECHHHHCCCCCCCCEEEECCEEECCCCCCCEEEHHHHHHHHHHHHH
VLHEEAIFPLRDKNIPINIKNTNAPDEAGTLILSKCDVKNKNIVTGISGKKDFTVINLEK
HHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCEEEEEEEE
VNMNTEKDFFRKLTTVFESNDILIEHMPSSIDSVSVLVSDSSITPKLNKVLEELKIYLDV
ECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHEEEEEE
DKISWERDISLIAVVGRGMINEKGVSSSTFTALAKEGVNIKMISQGSSEINIIIGVETKD
HHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEEECCCHH
FEKAIRSIYKEFYKED
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVVKFGGSSLADANQLKKVKDIIMNDDARKFIVVSAPGKGVNNKHKVTDLLAMCHELSA
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCC
HDLNFNEVFEIIENIYKNIVDDLVLNIDIDKILSDVKKEITNGASYDFVVSRGEFMSAKV
CCCCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
LAAYLDYDFVDAKDLIVFDDGELDIVKSHENIKNILTKHDRAVVPGFYGVDEYNNVHTFS
HHHHHCCCEECCCEEEEEECCCEEEEECHHHHHHHHHCCCCEECCCCCCCCCCCCEEEEC
RGGSDVTGSVIAASLDCEMYENFTDVSGFLVADPRIVKNPKPMGTITYHELRELSYMGAK
CCCCCCCCCEEEEEECHHHHCCCCCCCCEEEECCEEECCCCCCCEEEHHHHHHHHHHHHH
VLHEEAIFPLRDKNIPINIKNTNAPDEAGTLILSKCDVKNKNIVTGISGKKDFTVINLEK
HHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCEEEEEEEE
VNMNTEKDFFRKLTTVFESNDILIEHMPSSIDSVSVLVSDSSITPKLNKVLEELKIYLDV
ECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHEEEEEE
DKISWERDISLIAVVGRGMINEKGVSSSTFTALAKEGVNIKMISQGSSEINIIIGVETKD
HHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEEECCCHH
FEKAIRSIYKEFYKED
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]