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The map label for this gene is bsp6IM [H]

Identifier: 169823657

GI number: 169823657

Start: 133891

End: 134889

Strand: Reverse

Name: bsp6IM [H]

Synonym: FMG_P0143

Alternate gene names: 169823657

Gene position: 134889-133891 (Counterclockwise)

Preceding gene: 169823658

Following gene: 169823656

Centisome position: 71.31

GC content: 31.53

Gene sequence:

>999_bases
ATGAATAACAAAGTTATTAGCTTATTTGCTGGTGTAGGTGGAATTGATATAGGTTTTGAAAAGGCAGGATTTGAAACTAT
TTACGCTAACGAAATTGATGAAAAGGCTAGACAAACATATAAATTAAATTTTCCAGAAGTATTTTTGGATTCAAGAGATA
TACGAGATGTTCAAAAAGATGATATACCTCACGAGGCAAGTGTTGTTGTTAGCGGTTTTCCATGTCAGTCTTTTTCTATA
GCGGGATATAGAAAAGGATTAGAAGATGAAAAGAATGGTGATTTGTTTTTTGAGACATTACGAGTTGCTAAAGAGGTAGA
AGCTGATGTAATTTTCTTAGAAAACGTTAAAAACTTAGTAGGACATGATAGAGGTAAAACATTTAGGATAATGCTGGATG
CTTTAGAAAGCAATGGCTATTATGTAGACTATAAAGTATTAAATGCAACGGAGTTTGGGAATATACCTCAAAATAGAGAA
AGAATCTACATAGTTGCTTTTAGAGATAAAAATTGTCTAGATAACTTTGAGTTTCCTAAACCTATTGAATTAACAACAAC
AATTAGAGATTTTATAGATTTTGAAAATAAGAAAGATACTTCGTTTTACTATACTGAAAAAAGCTTCAAGCAATATGATG
AATTGAAATTTGGAATGGATGAACAAGATACTGTTTATCAATGGCGTAGGAAATATGTTAGAAAGAATATGAGTGGTGTT
TGTCCGACCCTAACAGCTAATATGGGAACAGGAGGACACAATGTGCCTCTTATTATTACCGATACAGGCGAATATAGGAA
ATTAACACCTAAAGAAACGTTCAATTTACAAGGATTTCCGAAGAATTATAAGCTTCCAGATATTGCTAAAAGCCATCTAT
ACAAACAGGCTGGTAATTCAGTTGTTGTTCCAGTAATAACAAGAATTGCAAAAAATATTATGAAAAGTATAGAAAAATGT
AATAAAAAAGAAGTTCAGAGAGAAACAGCCAGATGTTAA

Upstream 100 bases:

>100_bases
TGGTAGTTATGAAGAATTTGAAAGGGAGTATAAAAAATAATCATATTTTGGTTATTTTTTATGGTAATATTTGCGGCGTG
CCGCAATTAAGGAGAAAGTA

Downstream 100 bases:

>100_bases
TAGTCTGGTTGTTTTTTTGTTGTTATCAATATTTCGTAAAATTTTTTCTAAAATTTCCAACACCTCTTATTTGTGGGTAT
AACATTAGTGAACATTATAT

Product: cytosine-specific methyltransferase

Products: NA

Alternate protein names: M.Bsp6I; Cytosine-specific methyltransferase Bsp6I [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNNKVISLFAGVGGIDIGFEKAGFETIYANEIDEKARQTYKLNFPEVFLDSRDIRDVQKDDIPHEASVVVSGFPCQSFSI
AGYRKGLEDEKNGDLFFETLRVAKEVEADVIFLENVKNLVGHDRGKTFRIMLDALESNGYYVDYKVLNATEFGNIPQNRE
RIYIVAFRDKNCLDNFEFPKPIELTTTIRDFIDFENKKDTSFYYTEKSFKQYDELKFGMDEQDTVYQWRRKYVRKNMSGV
CPTLTANMGTGGHNVPLIITDTGEYRKLTPKETFNLQGFPKNYKLPDIAKSHLYKQAGNSVVVPVITRIAKNIMKSIEKC
NKKEVQRETARC

Sequences:

>Translated_332_residues
MNNKVISLFAGVGGIDIGFEKAGFETIYANEIDEKARQTYKLNFPEVFLDSRDIRDVQKDDIPHEASVVVSGFPCQSFSI
AGYRKGLEDEKNGDLFFETLRVAKEVEADVIFLENVKNLVGHDRGKTFRIMLDALESNGYYVDYKVLNATEFGNIPQNRE
RIYIVAFRDKNCLDNFEFPKPIELTTTIRDFIDFENKKDTSFYYTEKSFKQYDELKFGMDEQDTVYQWRRKYVRKNMSGV
CPTLTANMGTGGHNVPLIITDTGEYRKLTPKETFNLQGFPKNYKLPDIAKSHLYKQAGNSVVVPVITRIAKNIMKSIEKC
NKKEVQRETARC
>Mature_332_residues
MNNKVISLFAGVGGIDIGFEKAGFETIYANEIDEKARQTYKLNFPEVFLDSRDIRDVQKDDIPHEASVVVSGFPCQSFSI
AGYRKGLEDEKNGDLFFETLRVAKEVEADVIFLENVKNLVGHDRGKTFRIMLDALESNGYYVDYKVLNATEFGNIPQNRE
RIYIVAFRDKNCLDNFEFPKPIELTTTIRDFIDFENKKDTSFYYTEKSFKQYDELKFGMDEQDTVYQWRRKYVRKNMSGV
CPTLTANMGTGGHNVPLIITDTGEYRKLTPKETFNLQGFPKNYKLPDIAKSHLYKQAGNSVVVPVITRIAKNIMKSIEKC
NKKEVQRETARC

Specific function: This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=354, Percent_Identity=29.3785310734463, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 38222; Mature: 38222

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKVISLFAGVGGIDIGFEKAGFETIYANEIDEKARQTYKLNFPEVFLDSRDIRDVQKD
CCCCEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHEEECCCCHHCCCCCCHHHHHHC
DIPHEASVVVSGFPCQSFSIAGYRKGLEDEKNGDLFFETLRVAKEVEADVIFLENVKNLV
CCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEHHHHHHHH
GHDRGKTFRIMLDALESNGYYVDYKVLNATEFGNIPQNRERIYIVAFRDKNCLDNFEFPK
CCCCCCEEEEEEEEHHCCCEEEEEEEECCHHCCCCCCCCCEEEEEEECCCCCCCCCCCCC
PIELTTTIRDFIDFENKKDTSFYYTEKSFKQYDELKFGMDEQDTVYQWRRKYVRKNMSGV
CCCHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
CPTLTANMGTGGHNVPLIITDTGEYRKLTPKETFNLQGFPKNYKLPDIAKSHLYKQAGNS
CCEEEECCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
VVVPVITRIAKNIMKSIEKCNKKEVQRETARC
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNKVISLFAGVGGIDIGFEKAGFETIYANEIDEKARQTYKLNFPEVFLDSRDIRDVQKD
CCCCEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHEEECCCCHHCCCCCCHHHHHHC
DIPHEASVVVSGFPCQSFSIAGYRKGLEDEKNGDLFFETLRVAKEVEADVIFLENVKNLV
CCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEHHHHHHHH
GHDRGKTFRIMLDALESNGYYVDYKVLNATEFGNIPQNRERIYIVAFRDKNCLDNFEFPK
CCCCCCEEEEEEEEHHCCCEEEEEEEECCHHCCCCCCCCCEEEEEEECCCCCCCCCCCCC
PIELTTTIRDFIDFENKKDTSFYYTEKSFKQYDELKFGMDEQDTVYQWRRKYVRKNMSGV
CCCHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
CPTLTANMGTGGHNVPLIITDTGEYRKLTPKETFNLQGFPKNYKLPDIAKSHLYKQAGNS
CCEEEECCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
VVVPVITRIAKNIMKSIEKCNKKEVQRETARC
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607501 [H]