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The map label for this gene is iscS

Identifier: 167623503

GI number: 167623503

Start: 1890787

End: 1892001

Strand: Direct

Name: iscS

Synonym: Shal_1572

Alternate gene names: 167623503

Gene position: 1890787-1892001 (Clockwise)

Preceding gene: 167623502

Following gene: 167623504

Centisome position: 36.17

GC content: 46.34

Gene sequence:

>1215_bases
ATGAAGCTACCTATCTATTTAGATTATGCTGCGACGACGCCGGTTGACCCTCGTGTCGCAGAGAAAATGATGCAATGCCT
GACAATGGATGGCATTTTCGGTAATCCAGCGTCTCGTTCTCACCGTTACGGTTGGCAAGCTGAAGAGGCTGTTGATATCG
CTCGTAACCAAGTCGCTGATTTGATTAACGCCGATCCACGTGAAATCGTGTTTACTTCTGGTGCGACAGAGTCTGACAAC
TTAGCAATTAAAGGTGTTGCTCACTTCTATCATAAGAAGGGTAAGCACATCATCACTAGCAAAACTGAACACAAAGCAGT
GTTAGATACTTGCCGCCAGCTTGAGCGTGAAGGTTATGAAGTGACTTACCTTCAGCCAGAGCCAAGTGGTCTTATTCCTG
TCGCTATGATTGAAGCCGCCATGCGTGAAGACACTATTTTAGTGAGCATCATGCAGGTGAACAATGAAATCGGTGTGATT
CAAGATATCGATGCGATTGGTGAGCTATGTCGTTCACGCAAAATCATCTTCCACGTCGATGCGGCGCAAAGCGCTGGTAA
ATTACCTATCGATGTGCAAACCACTAAAGTCGATTTGATGTCTATCTCTGGACATAAAATGTACGGGCCTAAGGGTATTG
GTGCACTTTATGTTAGCCGTAAACCACGCATCCGCCTAGAAGCTGCTATGCATGGTGGTGGACATGAGCGTGGTATGCGT
AGTGGCACGCTTGCGACTCATCAAATTGTGGGTATGGGCGAAGCCGCGGCCATCGCTAAAGCCGATATGGTCGTTGATAA
CGAGCGTATTGCTCGTCTACGCGATAAGTTATGGAACGGCATCAAACACATCGAAGAAACCTATATTAATGGTGACGTGG
AAAAAAGAGCCTGTGGTAGCCTAAACGTCAGCTTTAACTTCGTTGAAGGTGAGTCTTTGATGATGGCATTGAAAGACTTA
GCGGTATCATCGGGTTCTGCCTGTACTTCTGCAAGCCTAGAGCCAAGCTATGTGCTACGTGCGCTAGGTCTTAATGACGA
GATGGCTCACAGCTCTATTCGATTCTCAATCGGCCGTTTCACGACTGATGAAGAGATCGACCATGCAATAGAAACGATTA
AAGAATCTATTGGTAATTTAAGGGAAATGTCTCCGCTTTGGGAAATGTTCAAAGACGGTATCGATCTGGACTCAGTCCAG
TGGGCACATCACTAA

Upstream 100 bases:

>100_bases
CAAAGAGTAAGTATTTAACTGCCTTTGTTATGAATATAAAAATTATTTTTTGTACGTTGTAAGTGGTCTCAGCTAGAGGC
TACAAAGTACGGAGTGTGTG

Downstream 100 bases:

>100_bases
CCTTAGCTTAATTCACGAATAGATAAATTGGAGTAGTATCATGGCTTATAGCGAAAAAGTAATCGATCATTATGAGAATC
CACGTAATGTGGGCTCATTC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYAATTPVDPRVAEKMMQCLTMDGIFGNPASRSHRYGWQAEEAVDIARNQVADLINADPREIVFTSGATESDN
LAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGYEVTYLQPEPSGLIPVAMIEAAMREDTILVSIMQVNNEIGVI
QDIDAIGELCRSRKIIFHVDAAQSAGKLPIDVQTTKVDLMSISGHKMYGPKGIGALYVSRKPRIRLEAAMHGGGHERGMR
SGTLATHQIVGMGEAAAIAKADMVVDNERIARLRDKLWNGIKHIEETYINGDVEKRACGSLNVSFNFVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTDEEIDHAIETIKESIGNLREMSPLWEMFKDGIDLDSVQ
WAHH

Sequences:

>Translated_404_residues
MKLPIYLDYAATTPVDPRVAEKMMQCLTMDGIFGNPASRSHRYGWQAEEAVDIARNQVADLINADPREIVFTSGATESDN
LAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGYEVTYLQPEPSGLIPVAMIEAAMREDTILVSIMQVNNEIGVI
QDIDAIGELCRSRKIIFHVDAAQSAGKLPIDVQTTKVDLMSISGHKMYGPKGIGALYVSRKPRIRLEAAMHGGGHERGMR
SGTLATHQIVGMGEAAAIAKADMVVDNERIARLRDKLWNGIKHIEETYINGDVEKRACGSLNVSFNFVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTDEEIDHAIETIKESIGNLREMSPLWEMFKDGIDLDSVQ
WAHH
>Mature_404_residues
MKLPIYLDYAATTPVDPRVAEKMMQCLTMDGIFGNPASRSHRYGWQAEEAVDIARNQVADLINADPREIVFTSGATESDN
LAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGYEVTYLQPEPSGLIPVAMIEAAMREDTILVSIMQVNNEIGVI
QDIDAIGELCRSRKIIFHVDAAQSAGKLPIDVQTTKVDLMSISGHKMYGPKGIGALYVSRKPRIRLEAAMHGGGHERGMR
SGTLATHQIVGMGEAAAIAKADMVVDNERIARLRDKLWNGIKHIEETYINGDVEKRACGSLNVSFNFVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTDEEIDHAIETIKESIGNLREMSPLWEMFKDGIDLDSVQ
WAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=58.7064676616915, Blast_Score=508, Evalue=1e-144,
Organism=Homo sapiens, GI156713448, Length=419, Percent_Identity=31.0262529832936, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=78.7128712871287, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=215, Percent_Identity=31.6279069767442, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787970, Length=224, Percent_Identity=32.1428571428571, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=58.8089330024814, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=32.9230769230769, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=57.6059850374065, Blast_Score=488, Evalue=1e-139,
Organism=Drosophila melanogaster, GI20129463, Length=405, Percent_Identity=57.2839506172839, Blast_Score=488, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_SHEHH (B0TNY1)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001673797.1
- ProteinModelPortal:   B0TNY1
- SMR:   B0TNY1
- GeneID:   5903137
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_1572
- HOGENOM:   HBG635316
- OMA:   DMSPLWD
- ProtClustDB:   PRK14012
- BioCyc:   SHAL458817:SHAL_1572-MONOMER
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR02006

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 44594; Mature: 44594

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYAATTPVDPRVAEKMMQCLTMDGIFGNPASRSHRYGWQAEEAVDIARNQVAD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADPREIVFTSGATESDNLAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGYE
HHCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLQPEPSGLIPVAMIEAAMREDTILVSIMQVNNEIGVIQDIDAIGELCRSRKIIFHVD
EEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEECHHHHHHHHHCCEEEEEEE
AAQSAGKLPIDVQTTKVDLMSISGHKMYGPKGIGALYVSRKPRIRLEAAMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCEEEEECCCCCEEEEEEECCCCHHCCCC
SGTLATHQIVGMGEAAAIAKADMVVDNERIARLRDKLWNGIKHIEETYINGDVEKRACGS
CCCCHHHHEECCCCHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
LNVSFNFVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
CEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEEECCC
TTDEEIDHAIETIKESIGNLREMSPLWEMFKDGIDLDSVQWAHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLPIYLDYAATTPVDPRVAEKMMQCLTMDGIFGNPASRSHRYGWQAEEAVDIARNQVAD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADPREIVFTSGATESDNLAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGYE
HHCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLQPEPSGLIPVAMIEAAMREDTILVSIMQVNNEIGVIQDIDAIGELCRSRKIIFHVD
EEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEECHHHHHHHHHCCEEEEEEE
AAQSAGKLPIDVQTTKVDLMSISGHKMYGPKGIGALYVSRKPRIRLEAAMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCEEEEECCCCCEEEEEEECCCCHHCCCC
SGTLATHQIVGMGEAAAIAKADMVVDNERIARLRDKLWNGIKHIEETYINGDVEKRACGS
CCCCHHHHEECCCCHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
LNVSFNFVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
CEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEEECCC
TTDEEIDHAIETIKESIGNLREMSPLWEMFKDGIDLDSVQWAHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA