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The map label for this gene is leuD

Identifier: 167622381

GI number: 167622381

Start: 518540

End: 519145

Strand: Direct

Name: leuD

Synonym: Shal_0441

Alternate gene names: 167622381

Gene position: 518540-519145 (Clockwise)

Preceding gene: 167622380

Following gene: 167622382

Centisome position: 9.92

GC content: 43.89

Gene sequence:

>606_bases
ATGCAACCATTTATTTCACATACAGGGCTTGCGGTTATCTTAGATAGTGCAAACGTCGATACCGACCAGATTATCCCTAA
ACAATTTTTGTCTAAGGTGACCCGAGACGGCTTTGGTGTTCACTTATTTCATGATTGGCGTTATCTCGATGAGGCTGGCG
ATAAGCCAAACCCAGATTTTAATCTGAATCAGCCTCGATATAAGGGGGCGTCTATTCTGGTGTCGCAGGAAAATTTTGGC
TGTGGTTCAAGCCGAGAACACGCGCCATGGGCATTGGCTGATTTTGGTTTTAAGGTGATTATTGCGCCTAGTTTTGCCGA
TATCTTTTATGGCAACTCAATCAACAATGGTTTGTTGCCTGTCAGGTTAACCGATGCCGAAGTTAAACAGCTTATGGCTG
AGGTTGAGGCTAAAGAAGGGGCTGAGATCGATGTTGATCTACAAGCATTGACTGTGACGAGTCCATCAGGCGCTTTATTC
CATTTTGAAATTGCTCAGTCGGCAAGGCATAACTTATTAAATGGATTAGATGCGATTGGGTTAACGCTGTCACACGTCGA
TACGATTGCTAATTATGAAGCTAACATTCCATCTTGGAGAGGCTGA

Upstream 100 bases:

>100_bases
GCGTGGTAGCCGTACTCACTTAGTGAGCCCTGCCATGGCGGCAGCTGCAGCCGTTGCAGGCCATTTTGTTGATATTCGTA
AACCATACTAGGAGAGCAGC

Downstream 100 bases:

>100_bases
TAGCATTGAAATAGTACCTAAAAGTTAGCGTACATATGTACCATTATTGAGTTTTGCTGAAGCCATTTAGATTTTTTATA
AATGGCTTTGTTTAATTTAG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MQPFISHTGLAVILDSANVDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDEAGDKPNPDFNLNQPRYKGASILVSQENFG
CGSSREHAPWALADFGFKVIIAPSFADIFYGNSINNGLLPVRLTDAEVKQLMAEVEAKEGAEIDVDLQALTVTSPSGALF
HFEIAQSARHNLLNGLDAIGLTLSHVDTIANYEANIPSWRG

Sequences:

>Translated_201_residues
MQPFISHTGLAVILDSANVDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDEAGDKPNPDFNLNQPRYKGASILVSQENFG
CGSSREHAPWALADFGFKVIIAPSFADIFYGNSINNGLLPVRLTDAEVKQLMAEVEAKEGAEIDVDLQALTVTSPSGALF
HFEIAQSARHNLLNGLDAIGLTLSHVDTIANYEANIPSWRG
>Mature_201_residues
MQPFISHTGLAVILDSANVDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDEAGDKPNPDFNLNQPRYKGASILVSQENFG
CGSSREHAPWALADFGFKVIIAPSFADIFYGNSINNGLLPVRLTDAEVKQLMAEVEAKEGAEIDVDLQALTVTSPSGALF
HFEIAQSARHNLLNGLDAIGLTLSHVDTIANYEANIPSWRG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=198, Percent_Identity=65.6565656565657, Blast_Score=269, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=48.4848484848485, Blast_Score=181, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_SHEHH (B0TQM3)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672675.1
- ProteinModelPortal:   B0TQM3
- SMR:   B0TQM3
- GeneID:   5905824
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0441
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   SHAL458817:SHAL_0441-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22037; Mature: 22037

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFISHTGLAVILDSANVDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDEAGDKPNPDF
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCC
NLNQPRYKGASILVSQENFGCGSSREHAPWALADFGFKVIIAPSFADIFYGNSINNGLLP
CCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHEECCCCCCCEEE
VRLTDAEVKQLMAEVEAKEGAEIDVDLQALTVTSPSGALFHFEIAQSARHNLLNGLDAIG
EEECHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHH
LTLSHVDTIANYEANIPSWRG
HHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQPFISHTGLAVILDSANVDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDEAGDKPNPDF
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCC
NLNQPRYKGASILVSQENFGCGSSREHAPWALADFGFKVIIAPSFADIFYGNSINNGLLP
CCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHEECCCCCCCEEE
VRLTDAEVKQLMAEVEAKEGAEIDVDLQALTVTSPSGALFHFEIAQSARHNLLNGLDAIG
EEECHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHH
LTLSHVDTIANYEANIPSWRG
HHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA