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The map label for this gene is menE [H]

Identifier: 167622235

GI number: 167622235

Start: 352696

End: 354228

Strand: Reverse

Name: menE [H]

Synonym: Shal_0294

Alternate gene names: 167622235

Gene position: 354228-352696 (Counterclockwise)

Preceding gene: 167622236

Following gene: 167622232

Centisome position: 6.78

GC content: 51.08

Gene sequence:

>1533_bases
ATGGATCTCTGCATGCAAACTAAAACGCTCTCCCCCATCCATCGAGTCGCACTGCGCAGCAGCACGAGCGTTGCCTATCG
ATTGATGGGGCAAAGTGTTAGCTACGGCGCTCTCAGCCAAAGTGCTATCGCTTTGGCCGAGCAGTTATCAGCCATGGGCC
TAAAACGCGGAGACCGCTTGGCTTGTATCGCGCATAACTGTCCGCAGCTATTGCAGCTGTACTGGGCCTGTGTCGACAGC
GGTATCATCTTTTGCCCCATCTCGCCGCGCTTTACCGCTAATCAAGTGGTTGAGTTGATGCAGAGCCATAATTATCACCA
CTACTGGCTAGAAGCCTCACAACAATCTGACTCCAGCCTTATTGATACGCTCAACCGTCAGCCACAGCTAAAAGCTATCT
CACTAGTCTTTAATCCGAGTTTGCAAGCGCAGCAAACAAGCGAGAAAGCGCAACTAACAAGCGAGAAAGCGCAACTAACA
GTCGAGCCAGCGCCAGTACTTGAGCCACACATCGCCGTCAACGCCATTCTCACCTCCGGCAGCAGCGGCACACCCAAAGC
GGCGGCCCATAGCCTAGCAAACCATATCGCCAGCGCCGCAGGCTCGAGTGAGTTAATAAAGTTAGCAGCTGAAGATAGCT
GGTTACTGTCACTGCCGCTGTTTCATATCGGCGGACTCGCCATTTTAAACCGCTGTGCCCTCGCTGGCGCCTGTGTAGTA
TTTGAAGACAAGAGTCTATCGCTGGCAGAGCAGCTAACCCGAGATAGAGTCACTCATCTGTCCTTGGTAAGCGCCCAGTT
ACAGCAACTGCTCAATATAAATGGCGATAGCGAAAACAATGCCAAAAGCGACTGCTTAAGCCAAGTCAAAGCCATGTTAC
TGGGCGGCGGCGCAATCTCAGCCGACCTGCTAACCGAATTAGCTAAGCGCAATATCAAGGCCTACACCAGCTATGGCATG
ACAGAGATGGGATCGCAGATCACCACAGGCATCGCCTGCACTGATGGCTCAAGCGGCAAGCTGTTATCGGGACGCGAGTT
GAAGATTATCGAGAGTGAAATATGGGTAAAAGGTGACTGTCTGTTTCTTGGCTATTTAACCGCAGAGGGTTTTAATCAGC
CCATTGATAAAGACGGCTGGTTCTACACCAAAGATTTAGGCCACTTTGATGATAACGGCAATCTATGCATCGACGGTCGA
GCCGATAACATGTTCATCAGCGGCGGCGAGAACATCCAACCAGAAGAGATAGAAGCCGCATTAAAGTTGCACCCGTTAAT
CAGCGATGCCATCGTTTTCCCCATCATTGATGCCAAGTTTGGCAATCTGCCAGCGGCCATTCTAAAACTGGTGAGTGGTA
CCAGTAATGACCAAGAGCAGTTGCCCTCGGAAGCCGAGTTAACTGCATTTCTAGCGAGCAAAATCGCCCGTTTCAAGCGA
CCAAGGCGTTATTACCCTTGGCCAGAGGTTAGCAGTGCTGGGATTAAGGTTATCCGCAAACAAGTGATTGCAGCAGTTAA
TCTCGCGAAATAG

Upstream 100 bases:

>100_bases
TAGGCTAGGTTTGGTTAGATTAGACTAGATGTGGCTAACATAAGACTAGAGTCGTAATAGCCTAGGTGGCGATACCCTAA
TCAGTCAGATAACTTCAATA

Downstream 100 bases:

>100_bases
TGAGTTGAATTTCAGTCAACTTTTGACGCTTCAATCAAGCTCTGCTTAATAAAGATCGTAAAGTGTCATTCCTCTGCAGA
GAGGAAACCTCGGGCTACAG

Product: O-succinylbenzoate-CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MDLCMQTKTLSPIHRVALRSSTSVAYRLMGQSVSYGALSQSAIALAEQLSAMGLKRGDRLACIAHNCPQLLQLYWACVDS
GIIFCPISPRFTANQVVELMQSHNYHHYWLEASQQSDSSLIDTLNRQPQLKAISLVFNPSLQAQQTSEKAQLTSEKAQLT
VEPAPVLEPHIAVNAILTSGSSGTPKAAAHSLANHIASAAGSSELIKLAAEDSWLLSLPLFHIGGLAILNRCALAGACVV
FEDKSLSLAEQLTRDRVTHLSLVSAQLQQLLNINGDSENNAKSDCLSQVKAMLLGGGAISADLLTELAKRNIKAYTSYGM
TEMGSQITTGIACTDGSSGKLLSGRELKIIESEIWVKGDCLFLGYLTAEGFNQPIDKDGWFYTKDLGHFDDNGNLCIDGR
ADNMFISGGENIQPEEIEAALKLHPLISDAIVFPIIDAKFGNLPAAILKLVSGTSNDQEQLPSEAELTAFLASKIARFKR
PRRYYPWPEVSSAGIKVIRKQVIAAVNLAK

Sequences:

>Translated_510_residues
MDLCMQTKTLSPIHRVALRSSTSVAYRLMGQSVSYGALSQSAIALAEQLSAMGLKRGDRLACIAHNCPQLLQLYWACVDS
GIIFCPISPRFTANQVVELMQSHNYHHYWLEASQQSDSSLIDTLNRQPQLKAISLVFNPSLQAQQTSEKAQLTSEKAQLT
VEPAPVLEPHIAVNAILTSGSSGTPKAAAHSLANHIASAAGSSELIKLAAEDSWLLSLPLFHIGGLAILNRCALAGACVV
FEDKSLSLAEQLTRDRVTHLSLVSAQLQQLLNINGDSENNAKSDCLSQVKAMLLGGGAISADLLTELAKRNIKAYTSYGM
TEMGSQITTGIACTDGSSGKLLSGRELKIIESEIWVKGDCLFLGYLTAEGFNQPIDKDGWFYTKDLGHFDDNGNLCIDGR
ADNMFISGGENIQPEEIEAALKLHPLISDAIVFPIIDAKFGNLPAAILKLVSGTSNDQEQLPSEAELTAFLASKIARFKR
PRRYYPWPEVSSAGIKVIRKQVIAAVNLAK
>Mature_510_residues
MDLCMQTKTLSPIHRVALRSSTSVAYRLMGQSVSYGALSQSAIALAEQLSAMGLKRGDRLACIAHNCPQLLQLYWACVDS
GIIFCPISPRFTANQVVELMQSHNYHHYWLEASQQSDSSLIDTLNRQPQLKAISLVFNPSLQAQQTSEKAQLTSEKAQLT
VEPAPVLEPHIAVNAILTSGSSGTPKAAAHSLANHIASAAGSSELIKLAAEDSWLLSLPLFHIGGLAILNRCALAGACVV
FEDKSLSLAEQLTRDRVTHLSLVSAQLQQLLNINGDSENNAKSDCLSQVKAMLLGGGAISADLLTELAKRNIKAYTSYGM
TEMGSQITTGIACTDGSSGKLLSGRELKIIESEIWVKGDCLFLGYLTAEGFNQPIDKDGWFYTKDLGHFDDNGNLCIDGR
ADNMFISGGENIQPEEIEAALKLHPLISDAIVFPIIDAKFGNLPAAILKLVSGTSNDQEQLPSEAELTAFLASKIARFKR
PRRYYPWPEVSSAGIKVIRKQVIAAVNLAK

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=533, Percent_Identity=22.7016885553471, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI115511026, Length=459, Percent_Identity=24.400871459695, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI122937307, Length=476, Percent_Identity=25.6302521008403, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI27477105, Length=133, Percent_Identity=36.0902255639098, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI58082049, Length=480, Percent_Identity=24.7916666666667, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788595, Length=339, Percent_Identity=37.1681415929204, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI145693145, Length=511, Percent_Identity=25.440313111546, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788107, Length=509, Percent_Identity=22.2003929273084, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1786810, Length=507, Percent_Identity=24.2603550295858, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=529, Percent_Identity=26.0869565217391, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32563687, Length=534, Percent_Identity=23.9700374531835, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=487, Percent_Identity=26.2833675564682, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=512, Percent_Identity=24.21875, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=487, Percent_Identity=25.8726899383984, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=482, Percent_Identity=25.103734439834, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71994703, Length=551, Percent_Identity=20.8711433756806, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994694, Length=551, Percent_Identity=20.8711433756806, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=551, Percent_Identity=20.8711433756806, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=515, Percent_Identity=22.3300970873786, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560140, Length=346, Percent_Identity=24.8554913294798, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=24.3016759776536, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=352, Percent_Identity=25, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24581924, Length=520, Percent_Identity=25.3846153846154, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922652, Length=468, Percent_Identity=25.2136752136752, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19921316, Length=475, Percent_Identity=24, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21356947, Length=439, Percent_Identity=23.4624145785877, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648260, Length=494, Percent_Identity=23.4817813765182, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355181, Length=473, Percent_Identity=23.2558139534884, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI18859661, Length=330, Percent_Identity=23.3333333333333, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356441, Length=437, Percent_Identity=22.6544622425629, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20130357, Length=298, Percent_Identity=26.8456375838926, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21358303, Length=473, Percent_Identity=22.1987315010571, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648257, Length=498, Percent_Identity=22.8915662650602, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 55108; Mature: 55108

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLCMQTKTLSPIHRVALRSSTSVAYRLMGQSVSYGALSQSAIALAEQLSAMGLKRGDRL
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
ACIAHNCPQLLQLYWACVDSGIIFCPISPRFTANQVVELMQSHNYHHYWLEASQQSDSSL
EEEECCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHH
IDTLNRQPQLKAISLVFNPSLQAQQTSEKAQLTSEKAQLTVEPAPVLEPHIAVNAILTSG
HHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECC
SSGTPKAAAHSLANHIASAAGSSELIKLAAEDSWLLSLPLFHIGGLAILNRCALAGACVV
CCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHHHCEEEE
FEDKSLSLAEQLTRDRVTHLSLVSAQLQQLLNINGDSENNAKSDCLSQVKAMLLGGGAIS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
ADLLTELAKRNIKAYTSYGMTEMGSQITTGIACTDGSSGKLLSGRELKIIESEIWVKGDC
HHHHHHHHHCCHHHHHHCCHHHHCCHHHCCEEECCCCCCCEECCCCEEEEECCEEEECCE
LFLGYLTAEGFNQPIDKDGWFYTKDLGHFDDNGNLCIDGRADNMFISGGENIQPEEIEAA
EEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHH
LKLHPLISDAIVFPIIDAKFGNLPAAILKLVSGTSNDQEQLPSEAELTAFLASKIARFKR
HHHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHCC
PRRYYPWPEVSSAGIKVIRKQVIAAVNLAK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLCMQTKTLSPIHRVALRSSTSVAYRLMGQSVSYGALSQSAIALAEQLSAMGLKRGDRL
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
ACIAHNCPQLLQLYWACVDSGIIFCPISPRFTANQVVELMQSHNYHHYWLEASQQSDSSL
EEEECCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHH
IDTLNRQPQLKAISLVFNPSLQAQQTSEKAQLTSEKAQLTVEPAPVLEPHIAVNAILTSG
HHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECC
SSGTPKAAAHSLANHIASAAGSSELIKLAAEDSWLLSLPLFHIGGLAILNRCALAGACVV
CCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHHHCEEEE
FEDKSLSLAEQLTRDRVTHLSLVSAQLQQLLNINGDSENNAKSDCLSQVKAMLLGGGAIS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
ADLLTELAKRNIKAYTSYGMTEMGSQITTGIACTDGSSGKLLSGRELKIIESEIWVKGDC
HHHHHHHHHCCHHHHHHCCHHHHCCHHHCCEEECCCCCCCEECCCCEEEEECCEEEECCE
LFLGYLTAEGFNQPIDKDGWFYTKDLGHFDDNGNLCIDGRADNMFISGGENIQPEEIEAA
EEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHH
LKLHPLISDAIVFPIIDAKFGNLPAAILKLVSGTSNDQEQLPSEAELTAFLASKIARFKR
HHHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHCC
PRRYYPWPEVSSAGIKVIRKQVIAAVNLAK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]