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The map label for this gene is plsB

Identifier: 167622212

GI number: 167622212

Start: 324601

End: 327024

Strand: Direct

Name: plsB

Synonym: Shal_0271

Alternate gene names: 167622212

Gene position: 324601-327024 (Clockwise)

Preceding gene: 167622211

Following gene: 167622213

Centisome position: 6.21

GC content: 44.6

Gene sequence:

>2424_bases
ATGTCCAAACAAGACTCTCTTTGGTTCAAATCTTTACGTTGGCTGCAGCGTAAATTAGTTCACACTGTCGTGGTGCCCCA
CGATCCATTCGACGATCTCAATTTAGATCCAAGTAAGCCACTCGTGTATGTAATGAAAACTGAATCTGTCAGTGACATTG
CTGCGCTTAGTGAGATGACGGAAAAACTTGGTTTACCAAGCCCTTACGAGGAGCTTGAGGTCAATGGTATTAGGGCGCCA
AGAGTGGTTTGTCTAGAGGGGAGTAAGCCTCTTTTTGGCCAGCGTGAAGGCGGAGAGCAATACATAGATTATTTCAAGAG
ACTGCTTAGCGTACATAAGCAAAATCTTGAATTAGATATTCAATTAGTACCTGTTAGTCTTTATTGGGGACGTACGCCTG
GTAAAGAAGACGATACTATGCGCGCTGCGGTACTCGAGCGCCAAAATCCGACTTGGCTACGCAAGTGCTTGATGATCCTT
TTCTTAGGGCGTCATAACTTTGTCCAGTTTTCTAATGCAGTCTCACTACGTTATATGGCTGATGAACATGGTACTGACAA
ACGTATCGCGCAAAAACTGGCTCGCGTAGCACGAGCTCATTTCGAACGTCAGCGTAAAGTGATGACGGGGCCACAGCTGC
CTAAACGTCAGGCCTTATTTCATGCGCTGCTAAAATCAGATTCGATTACCAAAGCGATTAAAGAGGAAGCGGCAAGCAAG
AAGATCTCTGAATCCGAAGCGCGTGCTAAGGCGATGGAGTATCTAGATGAAGTCGCTGCAGACTACTCAGATAGCTTAGT
GCGTATTGCCGAGCGCTTCTTAACGTGGCTTTGGAATAAGCTTTATAAAGGCATCAATATTAAAGGTGCTGAGCAGGTTA
GACAGTTGCACCACGATGGTCATGAGATTGTTTATGTACCGTGTCATCGTAGTCATATGGACTACTTACTGCTGTCTTAT
ATTCTCTATTATCAAGGTATGGTTCCGCCGCATATTGCTGCAGGTATTAACCTTAACTTCTGGCCTGCAGGCCCAGCGTT
CCGCCGTGGTGGTGCCTTCTTCATTCGCCGTAGTTTCGGTGGTAATAAGCTTTACACCGCAGTGTTCCGTGAATATCTCG
ATCAGTTATTTACTAAGGGTTATTCGGTAGAGTATTTCACTGAAGGTGGTCGTTCACGTACTGGTCGCTTGCTTGCACCT
AAAACGGGCATGCTTGCGATGACATTAAACAGTGTGCTGCGTGGGGTAGAGCGTCCAGTCACCTTAGTGCCTGTGTATTT
AGGCTATGACCATGTAATGGAAGTGGCAACTTATCATAAAGAGCTCAGCGGTAAGAAGAAAAAGAAAGAATCAGTTTGGC
AGATTTTTGGTGCTATCCGTAAGTTAGGTAACTTTGGTCAAGGTTATGTGAACTTCGGCGAGCCAATTACCTTGCATAAT
TTCTTGAATGAGCAAGTGCCTAGCTGGCGTGATGATATTGCTAAAGATCCCGATCAAAAACCAACTTGGTTAACACCTGT
CGTTAATACCTTGGCAAACCAAGTGATGACCAATATTAACGATGCTGCGGCGGTAAGCTCGGTGACGTTAACCAGTATGG
TGTTGCTGGCTTCTGAGCAAAATGCGTTGGAGCGCTCTCAGTTAGAGAAGCAGCTAGATCTGTATTTAACCTTGCTCAAA
GAGCGACCATATACTGACTATACCTCTGTGCCTGATGGAACGGGTCATGACTTGGTGTCTCAAGGTTTAGAGCTGAAAAA
GCTGCAGATTGAAAGTGATCCGCTAGGCGATATCATCTCAATCGATCAAAGTATTGCGATCACCATGACTTATTATCGCA
ACAACATCATTCACCTTATGGTTCTACCGTCTTTAATCGCCGCCTGTTTGCTGCGTAAAGAGAACTGTGGCCGTAACGAC
GTTATTAGTATTGTAAATGACTTCTACCCACTGCTTGAAGCTGAGCTATTTATGGGGATTGAAGATCCAAGTCAGTATGC
AAACCAGATCCTCGATATCTTAGTGGCTCAAGGGCTAGTTGTTGAATGCGATCATTTTGAGGTTGTTGATAGCAGTATCA
ACCAGTTGTTACTCTTGTCAGGCACTATCAGCGAAACCATGCAGCGTTATGCAATTTTGTTTAACTTACTTGAAGTTAAG
CCAAATATGGAGCGCTCTGAGCTTGAGAAAGACAGCCACCGCCTGGCACAAAGATTAGGCGCACTTCACGGTATTACAGC
ACCAGAGTTTTATGACAAGAAGCTATACGCTACGCTGAGTGTCAAATTAAAAGAGTTAGGTTACCTAACTGATAATCAAG
GGTGTAGCGATATCAAACGTATTAAAGAAAGAGCGAACTTGTTGTTACGTTCATCAGTGAAGCAGACCATTGTCGATAGT
GTTCACGCGGAGCACATCGCTTAA

Upstream 100 bases:

>100_bases
ACAAACAGGATAGAGTGCGGTAACAAGAGAATCGCGCAAACTGTTTCAATATCTGGTATTCTAGCGCCTTATTAGAAGCG
ACTAACATTGCATATAAATC

Downstream 100 bases:

>100_bases
TGGCTAATCATATGAATGCCGCGGGTCATGAGACCAGCGGCAAGTCTCTGCTGGGCGGAGCAATGATCATCGCGGGCACG
GCTGTCGGCGCGGGTATGTT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MSKQDSLWFKSLRWLQRKLVHTVVVPHDPFDDLNLDPSKPLVYVMKTESVSDIAALSEMTEKLGLPSPYEELEVNGIRAP
RVVCLEGSKPLFGQREGGEQYIDYFKRLLSVHKQNLELDIQLVPVSLYWGRTPGKEDDTMRAAVLERQNPTWLRKCLMIL
FLGRHNFVQFSNAVSLRYMADEHGTDKRIAQKLARVARAHFERQRKVMTGPQLPKRQALFHALLKSDSITKAIKEEAASK
KISESEARAKAMEYLDEVAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQVRQLHHDGHEIVYVPCHRSHMDYLLLSY
ILYYQGMVPPHIAAGINLNFWPAGPAFRRGGAFFIRRSFGGNKLYTAVFREYLDQLFTKGYSVEYFTEGGRSRTGRLLAP
KTGMLAMTLNSVLRGVERPVTLVPVYLGYDHVMEVATYHKELSGKKKKKESVWQIFGAIRKLGNFGQGYVNFGEPITLHN
FLNEQVPSWRDDIAKDPDQKPTWLTPVVNTLANQVMTNINDAAAVSSVTLTSMVLLASEQNALERSQLEKQLDLYLTLLK
ERPYTDYTSVPDGTGHDLVSQGLELKKLQIESDPLGDIISIDQSIAITMTYYRNNIIHLMVLPSLIAACLLRKENCGRND
VISIVNDFYPLLEAELFMGIEDPSQYANQILDILVAQGLVVECDHFEVVDSSINQLLLLSGTISETMQRYAILFNLLEVK
PNMERSELEKDSHRLAQRLGALHGITAPEFYDKKLYATLSVKLKELGYLTDNQGCSDIKRIKERANLLLRSSVKQTIVDS
VHAEHIA

Sequences:

>Translated_807_residues
MSKQDSLWFKSLRWLQRKLVHTVVVPHDPFDDLNLDPSKPLVYVMKTESVSDIAALSEMTEKLGLPSPYEELEVNGIRAP
RVVCLEGSKPLFGQREGGEQYIDYFKRLLSVHKQNLELDIQLVPVSLYWGRTPGKEDDTMRAAVLERQNPTWLRKCLMIL
FLGRHNFVQFSNAVSLRYMADEHGTDKRIAQKLARVARAHFERQRKVMTGPQLPKRQALFHALLKSDSITKAIKEEAASK
KISESEARAKAMEYLDEVAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQVRQLHHDGHEIVYVPCHRSHMDYLLLSY
ILYYQGMVPPHIAAGINLNFWPAGPAFRRGGAFFIRRSFGGNKLYTAVFREYLDQLFTKGYSVEYFTEGGRSRTGRLLAP
KTGMLAMTLNSVLRGVERPVTLVPVYLGYDHVMEVATYHKELSGKKKKKESVWQIFGAIRKLGNFGQGYVNFGEPITLHN
FLNEQVPSWRDDIAKDPDQKPTWLTPVVNTLANQVMTNINDAAAVSSVTLTSMVLLASEQNALERSQLEKQLDLYLTLLK
ERPYTDYTSVPDGTGHDLVSQGLELKKLQIESDPLGDIISIDQSIAITMTYYRNNIIHLMVLPSLIAACLLRKENCGRND
VISIVNDFYPLLEAELFMGIEDPSQYANQILDILVAQGLVVECDHFEVVDSSINQLLLLSGTISETMQRYAILFNLLEVK
PNMERSELEKDSHRLAQRLGALHGITAPEFYDKKLYATLSVKLKELGYLTDNQGCSDIKRIKERANLLLRSSVKQTIVDS
VHAEHIA
>Mature_806_residues
SKQDSLWFKSLRWLQRKLVHTVVVPHDPFDDLNLDPSKPLVYVMKTESVSDIAALSEMTEKLGLPSPYEELEVNGIRAPR
VVCLEGSKPLFGQREGGEQYIDYFKRLLSVHKQNLELDIQLVPVSLYWGRTPGKEDDTMRAAVLERQNPTWLRKCLMILF
LGRHNFVQFSNAVSLRYMADEHGTDKRIAQKLARVARAHFERQRKVMTGPQLPKRQALFHALLKSDSITKAIKEEAASKK
ISESEARAKAMEYLDEVAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQVRQLHHDGHEIVYVPCHRSHMDYLLLSYI
LYYQGMVPPHIAAGINLNFWPAGPAFRRGGAFFIRRSFGGNKLYTAVFREYLDQLFTKGYSVEYFTEGGRSRTGRLLAPK
TGMLAMTLNSVLRGVERPVTLVPVYLGYDHVMEVATYHKELSGKKKKKESVWQIFGAIRKLGNFGQGYVNFGEPITLHNF
LNEQVPSWRDDIAKDPDQKPTWLTPVVNTLANQVMTNINDAAAVSSVTLTSMVLLASEQNALERSQLEKQLDLYLTLLKE
RPYTDYTSVPDGTGHDLVSQGLELKKLQIESDPLGDIISIDQSIAITMTYYRNNIIHLMVLPSLIAACLLRKENCGRNDV
ISIVNDFYPLLEAELFMGIEDPSQYANQILDILVAQGLVVECDHFEVVDSSINQLLLLSGTISETMQRYAILFNLLEVKP
NMERSELEKDSHRLAQRLGALHGITAPEFYDKKLYATLSVKLKELGYLTDNQGCSDIKRIKERANLLLRSSVKQTIVDSV
HAEHIA

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family

Homologues:

Organism=Homo sapiens, GI7657134, Length=447, Percent_Identity=29.9776286353468, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI190358539, Length=421, Percent_Identity=26.1282660332542, Blast_Score=140, Evalue=4e-33,
Organism=Escherichia coli, GI87082362, Length=809, Percent_Identity=47.9604449938195, Blast_Score=770, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=574, Percent_Identity=27.5261324041812, Blast_Score=161, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71988723, Length=464, Percent_Identity=26.0775862068966, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71988728, Length=389, Percent_Identity=28.2776349614396, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17864692, Length=327, Percent_Identity=31.1926605504587, Blast_Score=154, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24650754, Length=419, Percent_Identity=27.4463007159904, Blast_Score=129, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21357731, Length=419, Percent_Identity=27.4463007159904, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24650752, Length=419, Percent_Identity=27.4463007159904, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSB_SHEHH (B0TNU4)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672506.1
- ProteinModelPortal:   B0TNU4
- GeneID:   5906541
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0271
- HOGENOM:   HBG296590
- OMA:   VIYVPCH
- ProtClustDB:   PRK04974
- BioCyc:   SHAL458817:SHAL_0271-MONOMER
- HAMAP:   MF_00393
- InterPro:   IPR002123
- InterPro:   IPR022284
- SMART:   SM00563
- TIGRFAMs:   TIGR03703

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.15

Molecular weight: Translated: 91710; Mature: 91579

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQDSLWFKSLRWLQRKLVHTVVVPHDPFDDLNLDPSKPLVYVMKTESVSDIAALSEMT
CCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
EKLGLPSPYEELEVNGIRAPRVVCLEGSKPLFGQREGGEQYIDYFKRLLSVHKQNLELDI
HHCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEE
QLVPVSLYWGRTPGKEDDTMRAAVLERQNPTWLRKCLMILFLGRHNFVQFSNAVSLRYMA
EEEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEC
DEHGTDKRIAQKLARVARAHFERQRKVMTGPQLPKRQALFHALLKSDSITKAIKEEAASK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
KISESEARAKAMEYLDEVAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQVRQLHHDG
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
HEIVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPAFRRGGAFFIRRSFG
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCCHHHHCCCEEEEEECCC
GNKLYTAVFREYLDQLFTKGYSVEYFTEGGRSRTGRLLAPKTGMLAMTLNSVLRGVERPV
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCE
TLVPVYLGYDHVMEVATYHKELSGKKKKKESVWQIFGAIRKLGNFGQGYVNFGEPITLHN
EEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
FLNEQVPSWRDDIAKDPDQKPTWLTPVVNTLANQVMTNINDAAAVSSVTLTSMVLLASEQ
HHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCH
NALERSQLEKQLDLYLTLLKERPYTDYTSVPDGTGHDLVSQGLELKKLQIESDPLGDIIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCHHC
IDQSIAITMTYYRNNIIHLMVLPSLIAACLLRKENCGRNDVISIVNDFYPLLEAELFMGI
CCCCEEEEEEEHHCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
EDPSQYANQILDILVAQGLVVECDHFEVVDSSINQLLLLSGTISETMQRYAILFNLLEVK
CCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCC
PNMERSELEKDSHRLAQRLGALHGITAPEFYDKKLYATLSVKLKELGYLTDNQGCSDIKR
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
IKERANLLLRSSVKQTIVDSVHAEHIA
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKQDSLWFKSLRWLQRKLVHTVVVPHDPFDDLNLDPSKPLVYVMKTESVSDIAALSEMT
CCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
EKLGLPSPYEELEVNGIRAPRVVCLEGSKPLFGQREGGEQYIDYFKRLLSVHKQNLELDI
HHCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEE
QLVPVSLYWGRTPGKEDDTMRAAVLERQNPTWLRKCLMILFLGRHNFVQFSNAVSLRYMA
EEEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEC
DEHGTDKRIAQKLARVARAHFERQRKVMTGPQLPKRQALFHALLKSDSITKAIKEEAASK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
KISESEARAKAMEYLDEVAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQVRQLHHDG
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
HEIVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPAFRRGGAFFIRRSFG
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCCHHHHCCCEEEEEECCC
GNKLYTAVFREYLDQLFTKGYSVEYFTEGGRSRTGRLLAPKTGMLAMTLNSVLRGVERPV
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCE
TLVPVYLGYDHVMEVATYHKELSGKKKKKESVWQIFGAIRKLGNFGQGYVNFGEPITLHN
EEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
FLNEQVPSWRDDIAKDPDQKPTWLTPVVNTLANQVMTNINDAAAVSSVTLTSMVLLASEQ
HHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCH
NALERSQLEKQLDLYLTLLKERPYTDYTSVPDGTGHDLVSQGLELKKLQIESDPLGDIIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCHHC
IDQSIAITMTYYRNNIIHLMVLPSLIAACLLRKENCGRNDVISIVNDFYPLLEAELFMGI
CCCCEEEEEEEHHCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
EDPSQYANQILDILVAQGLVVECDHFEVVDSSINQLLLLSGTISETMQRYAILFNLLEVK
CCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCC
PNMERSELEKDSHRLAQRLGALHGITAPEFYDKKLYATLSVKLKELGYLTDNQGCSDIKR
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
IKERANLLLRSSVKQTIVDSVHAEHIA
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA