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The map label for this gene is caiA [H]

Identifier: 167622162

GI number: 167622162

Start: 267141

End: 268277

Strand: Direct

Name: caiA [H]

Synonym: Shal_0221

Alternate gene names: 167622162

Gene position: 267141-268277 (Clockwise)

Preceding gene: 167622161

Following gene: 167622163

Centisome position: 5.11

GC content: 48.28

Gene sequence:

>1137_bases
ATGGATTTTAAATTAACCGACGAACAAGAGTTATTTGTAGCAGGTATCCGTGACCTGATGGCTAAAGAGAACTGGGAAAG
CTATTTCGCAGAATGTGATGAGAATAGTCAGTATCCAGAGCGCTTCGTTAAAGAGCTAGCCGAGATGGGTATCGACAGCT
TGTTGTTGCCAGAGGAGCACGGCGGATTCAACGAAGGCATGGTAACGCTAGCGGTGATCTGGGAAGAGTTAGGTCGCTTA
GGTGCGCCAACTTACGTGCTATATCAGTTGCCAGGTATCACTACCATGCTAAGACACGGTAGCCCTGAGCAGATCGATAA
GATCATGACGCTACGTGGTACGGGTAAGCAGATGTGGAACTCAGCTATCACTGAGCCAAGTGCGGGTTCTGACGTGGGTA
GCCTACTAACCACTTATAAGCGTCGTGATGGCAAGGTGTATTTAACTGGTAGTAAGTGCTTCATCACTAGTGCTGCAGGT
GCTCCTTACATCGTGGTTATGGCTAAAGATGCTGACTCAGAAAAGCCAATCTTCACCGAGTGGTTCGTCGATATGTCTAA
GCCAGGTATCAAGCTAAGCAAGCTACCAAAGCTAGGCTTAAGAATGGATAGCTGTTGTGAGCTACAGTTTGACGAGTTTG
AGCTAGAAGAGTCAGACATGTTTGGTATCGAAGGCAATGGCTTCATGCGTGTTAAAGAGGAGTTTGACTCTGAGCGCTTC
CTTGTGGCCCTAACTAACTACGGCGCGGCTTACTGTGCATTCGAAGATGCGGCTAAGTACGCCAACCAACGTGTTCAGTT
CGGTGAGGCTATTGGTCGTTACCAGTTGATCCAAGAGAAGTTTGCCCACATGGCTATCAAGCTTAACAGCATGAAGAACA
TGGTCTATGAGGCGGCGTGGAAGTGTGACAACGGCACTATGACATCGGGCGATTCGGCAATGTGTAAGTTCTACTGCGCC
AATGCCGGCTTCGAAGTTATCGACTCTGCTATGCAAGTACTAGGTGGTTACGCGATTGCTGGTGAGCACCGTATCAGCCG
TATGTGGCGTGATATTCGTGTTGACCGTGTCTCAGGTGGTTCTGATGAAATGCAGATCTTAACCTTAGGCCGTGAAGTAC
TGAAGCGCTATCGTTAA

Upstream 100 bases:

>100_bases
TCTCTTTCTTAAAAGATGCCCGTAAAAATAATTGGAAACCTGTCGGTGAAGAGGCTAAGCCACACCTAGGTTCCACAGCT
AAAATTTAGAGGAATAAATA

Downstream 100 bases:

>100_bases
TAAACCGCGGTGATAAACAGGCTAGGTACTGAGTTAGTGACTATTGAGCTAGTAAGTAGTGAGCTAGTAAGTACTTAGTC
TGGGTCGATTCTAGTGTGGG

Product: crotonobetainyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA reductase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MDFKLTDEQELFVAGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEHGGFNEGMVTLAVIWEELGRL
GAPTYVLYQLPGITTMLRHGSPEQIDKIMTLRGTGKQMWNSAITEPSAGSDVGSLLTTYKRRDGKVYLTGSKCFITSAAG
APYIVVMAKDADSEKPIFTEWFVDMSKPGIKLSKLPKLGLRMDSCCELQFDEFELEESDMFGIEGNGFMRVKEEFDSERF
LVALTNYGAAYCAFEDAAKYANQRVQFGEAIGRYQLIQEKFAHMAIKLNSMKNMVYEAAWKCDNGTMTSGDSAMCKFYCA
NAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGGSDEMQILTLGREVLKRYR

Sequences:

>Translated_378_residues
MDFKLTDEQELFVAGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEHGGFNEGMVTLAVIWEELGRL
GAPTYVLYQLPGITTMLRHGSPEQIDKIMTLRGTGKQMWNSAITEPSAGSDVGSLLTTYKRRDGKVYLTGSKCFITSAAG
APYIVVMAKDADSEKPIFTEWFVDMSKPGIKLSKLPKLGLRMDSCCELQFDEFELEESDMFGIEGNGFMRVKEEFDSERF
LVALTNYGAAYCAFEDAAKYANQRVQFGEAIGRYQLIQEKFAHMAIKLNSMKNMVYEAAWKCDNGTMTSGDSAMCKFYCA
NAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGGSDEMQILTLGREVLKRYR
>Mature_378_residues
MDFKLTDEQELFVAGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEHGGFNEGMVTLAVIWEELGRL
GAPTYVLYQLPGITTMLRHGSPEQIDKIMTLRGTGKQMWNSAITEPSAGSDVGSLLTTYKRRDGKVYLTGSKCFITSAAG
APYIVVMAKDADSEKPIFTEWFVDMSKPGIKLSKLPKLGLRMDSCCELQFDEFELEESDMFGIEGNGFMRVKEEFDSERF
LVALTNYGAAYCAFEDAAKYANQRVQFGEAIGRYQLIQEKFAHMAIKLNSMKNMVYEAAWKCDNGTMTSGDSAMCKFYCA
NAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGGSDEMQILTLGREVLKRYR

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma- butyrobetainyl-CoA [H]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=382, Percent_Identity=29.5811518324607, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI226958412, Length=368, Percent_Identity=27.9891304347826, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI226958414, Length=339, Percent_Identity=28.3185840707965, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI4557231, Length=383, Percent_Identity=27.154046997389, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI187960098, Length=383, Percent_Identity=27.154046997389, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI4501859, Length=378, Percent_Identity=25.3968253968254, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI4501857, Length=376, Percent_Identity=25.2659574468085, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI21361497, Length=357, Percent_Identity=28.8515406162465, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI7656849, Length=375, Percent_Identity=25.6, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI76496475, Length=332, Percent_Identity=28.6144578313253, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI4557235, Length=332, Percent_Identity=28.6144578313253, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI38505218, Length=343, Percent_Identity=23.6151603498542, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4503943, Length=376, Percent_Identity=22.3404255319149, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1786223, Length=380, Percent_Identity=80.5263157894737, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI87081958, Length=382, Percent_Identity=47.3821989528796, Blast_Score=355, Evalue=4e-99,
Organism=Escherichia coli, GI87082384, Length=263, Percent_Identity=26.9961977186312, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17538396, Length=382, Percent_Identity=31.6753926701571, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17569725, Length=357, Percent_Identity=27.7310924369748, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17570075, Length=383, Percent_Identity=26.6318537859008, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17534899, Length=385, Percent_Identity=26.2337662337662, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17508101, Length=381, Percent_Identity=24.9343832020997, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17533517, Length=362, Percent_Identity=27.3480662983425, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506239, Length=380, Percent_Identity=25.5263157894737, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17551932, Length=365, Percent_Identity=24.6575342465753, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71985184, Length=377, Percent_Identity=27.5862068965517, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17505929, Length=365, Percent_Identity=24.6575342465753, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71985192, Length=317, Percent_Identity=27.7602523659306, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71990804, Length=294, Percent_Identity=25.1700680272109, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32563615, Length=230, Percent_Identity=29.1304347826087, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17534353, Length=284, Percent_Identity=25, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355753, Length=344, Percent_Identity=29.9418604651163, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI21356377, Length=378, Percent_Identity=28.3068783068783, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24646207, Length=347, Percent_Identity=27.6657060518732, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24666513, Length=380, Percent_Identity=26.8421052631579, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281363737, Length=353, Percent_Identity=29.7450424929178, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24660351, Length=378, Percent_Identity=26.1904761904762, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19920834, Length=279, Percent_Identity=26.5232974910394, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42598; Mature: 42598

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00072 ACYL_COA_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKLTDEQELFVAGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEH
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCC
GGFNEGMVTLAVIWEELGRLGAPTYVLYQLPGITTMLRHGSPEQIDKIMTLRGTGKQMWN
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCHHHHH
SAITEPSAGSDVGSLLTTYKRRDGKVYLTGSKCFITSAAGAPYIVVMAKDADSEKPIFTE
HHCCCCCCCCHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCEEEEEECCCCCCCCCHHH
WFVDMSKPGIKLSKLPKLGLRMDSCCELQFDEFELEESDMFGIEGNGFMRVKEEFDSERF
HHHHCCCCCCCHHCCCCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEHHHCCCCEE
LVALTNYGAAYCAFEDAAKYANQRVQFGEAIGRYQLIQEKFAHMAIKLNSMKNMVYEAAW
EEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KCDNGTMTSGDSAMCKFYCANAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGG
ECCCCCCCCCCHHEEEEEECCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHEECCCC
SDEMQILTLGREVLKRYR
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFKLTDEQELFVAGIRDLMAKENWESYFAECDENSQYPERFVKELAEMGIDSLLLPEEH
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCC
GGFNEGMVTLAVIWEELGRLGAPTYVLYQLPGITTMLRHGSPEQIDKIMTLRGTGKQMWN
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCHHHHH
SAITEPSAGSDVGSLLTTYKRRDGKVYLTGSKCFITSAAGAPYIVVMAKDADSEKPIFTE
HHCCCCCCCCHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCEEEEEECCCCCCCCCHHH
WFVDMSKPGIKLSKLPKLGLRMDSCCELQFDEFELEESDMFGIEGNGFMRVKEEFDSERF
HHHHCCCCCCCHHCCCCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEHHHCCCCEE
LVALTNYGAAYCAFEDAAKYANQRVQFGEAIGRYQLIQEKFAHMAIKLNSMKNMVYEAAW
EEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KCDNGTMTSGDSAMCKFYCANAGFEVIDSAMQVLGGYAIAGEHRISRMWRDIRVDRVSGG
ECCCCCCCCCCHHEEEEEECCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHEECCCC
SDEMQILTLGREVLKRYR
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA