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The map label for this gene is narH [H]

Identifier: 167622119

GI number: 167622119

Start: 210840

End: 212378

Strand: Direct

Name: narH [H]

Synonym: Shal_0178

Alternate gene names: 167622119

Gene position: 210840-212378 (Clockwise)

Preceding gene: 167622118

Following gene: 167622120

Centisome position: 4.03

GC content: 47.37

Gene sequence:

>1539_bases
ATGAAAATACGTTCTCAAATAGGTATGGTACTTAACCTTGATAAGTGTATCGGTTGCCATACTTGTTCAATTACTTGTAA
AAACGTATGGACGTCTCGCGAAGGCGTTGAGTACGCTTGGTTCAACAACGTTGAAACTAAGCCCGGTATTGGTTACCCCA
AAGAGTGGGAAAACCAAGATAAGTGGAATGGCGGTTGGGAGAGAAAGTCTAACGGTGATATTCAGCCTCGCATCGGTGGT
AAGTATCGCATATTGGCTAACATCTTTGCTAACCCTGATATGCCAGAGATCGATGACTACTACGAGCCATTCGATTATGA
CTATCAGCACCTGCACACAGCACCGATTGTTGAGCACCAACCTACGGCGCGTCCACGTTCTGTCATCTCTGGTAAGCGTA
TGGAGAAGATCGAGTGGGGTCCAAACTGGGAAGAGATCTTAGGTACTGAGTTCGAGAAGCGCAGTAAAGATAAGAACTTC
GACAACATGCAGAAAGATATGTATGGCGAGTTCGAAAATACATTCATGATGTATCTTCCCCGTCTTTGTGAGCACTGCTT
GAACCCAGCCTGTGTGGCTTCATGTCCATCGGGCGCAATCTATAAGCGTGAAGAGGACGGTATCGTTCTTATCGACCAAG
AGAAGTGTCGTGGTTGGAGAATGTGTATCTCAGGTTGCCCATACAAGAAGATCTACTACAACTGGAAGTCTGGTAAGTCA
GAGAAGTGTATCTTCTGTTACCCGCGTATTGAAGCCGGTATGCCGACAGTCTGTTCAGAGACCTGTGTAGGTCGTATCCG
TTACTTAGGTGTACTACTTTATGATGCAGACAAGATCAAAGAGGTGGCATCGACCGCATCTGAGCAGGATCTGTATGAGA
AGCAGTTGGAAGTTTTCTTAGACCCATTCGATCCAGCAGTGATTGAGCAGGCTCGTAAGGACGGCGTTGCAGATTCTGTC
ATCCAGGCGGCTCAAAAGTCGCCAGTTTACAAGATGGCGATGGATTGGAAACTGGCACTGCCACTACACCCAGAGTACCG
CACGCTACCTATGGTTTGGTATATCCCATCTCTGAGCCCAATTCAGAATGCAGCTGAGTCTGGGACCTTAGGTAAAGCGG
GTGTCATTCCTGATGTACGCTCACTGCGTATTCCTGTTAAGTACCTAGCTAACTTGCTAACCGCAGGTGATGAGGCTCCG
GTTATTACTGCGCTTGAGCGTATGCTGGCAATGCGTGCTTATAAGCGTGCAGAGAATGTCGAAGGTGTCGAAGACCTAGA
GGTTCTACAGCAAGTGGGTCTTACCAAGAAGCAGGCTGATGAGATGTATCGTTATCTTGCAATCGCTAACTACGAAGATC
GTTACGTTATCCCAAGTGGACACCGTGAGATGAACGTACCAGAGGCCTATGGTGAGAAGAGCGGTTGTGGCTTCACCTTT
GGTAATGGCTGTAGCGATGGCCATAACGGCGTCAATATGTTCGGCGGAAAGAAGATTACTCGCCGTAATATCATTAAAAC
TGTGAGTGTGGAGGACTAA

Upstream 100 bases:

>100_bases
TTAGAAAGATGGATAAAGTTGATTGGTTAGAGGGTTCTAATACCGATGACCTGCCGCGTCCATTGCCAACCAACCCAGAT
GAAGCTTAAGGAGTAGAACC

Downstream 100 bases:

>100_bases
TACTGTGCTAATCCTTAAAGTTGTTTCACGTCTACTCGATTATCCAACAGAGGCACTATTTGGTGCTGCCGACGAGATGA
TCGCTATTGTGGAGCAGTCG

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKIRSQIGMVLNLDKCIGCHTCSITCKNVWTSREGVEYAWFNNVETKPGIGYPKEWENQDKWNGGWERKSNGDIQPRIGG
KYRILANIFANPDMPEIDDYYEPFDYDYQHLHTAPIVEHQPTARPRSVISGKRMEKIEWGPNWEEILGTEFEKRSKDKNF
DNMQKDMYGEFENTFMMYLPRLCEHCLNPACVASCPSGAIYKREEDGIVLIDQEKCRGWRMCISGCPYKKIYYNWKSGKS
EKCIFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDADKIKEVASTASEQDLYEKQLEVFLDPFDPAVIEQARKDGVADSV
IQAAQKSPVYKMAMDWKLALPLHPEYRTLPMVWYIPSLSPIQNAAESGTLGKAGVIPDVRSLRIPVKYLANLLTAGDEAP
VITALERMLAMRAYKRAENVEGVEDLEVLQQVGLTKKQADEMYRYLAIANYEDRYVIPSGHREMNVPEAYGEKSGCGFTF
GNGCSDGHNGVNMFGGKKITRRNIIKTVSVED

Sequences:

>Translated_512_residues
MKIRSQIGMVLNLDKCIGCHTCSITCKNVWTSREGVEYAWFNNVETKPGIGYPKEWENQDKWNGGWERKSNGDIQPRIGG
KYRILANIFANPDMPEIDDYYEPFDYDYQHLHTAPIVEHQPTARPRSVISGKRMEKIEWGPNWEEILGTEFEKRSKDKNF
DNMQKDMYGEFENTFMMYLPRLCEHCLNPACVASCPSGAIYKREEDGIVLIDQEKCRGWRMCISGCPYKKIYYNWKSGKS
EKCIFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDADKIKEVASTASEQDLYEKQLEVFLDPFDPAVIEQARKDGVADSV
IQAAQKSPVYKMAMDWKLALPLHPEYRTLPMVWYIPSLSPIQNAAESGTLGKAGVIPDVRSLRIPVKYLANLLTAGDEAP
VITALERMLAMRAYKRAENVEGVEDLEVLQQVGLTKKQADEMYRYLAIANYEDRYVIPSGHREMNVPEAYGEKSGCGFTF
GNGCSDGHNGVNMFGGKKITRRNIIKTVSVED
>Mature_512_residues
MKIRSQIGMVLNLDKCIGCHTCSITCKNVWTSREGVEYAWFNNVETKPGIGYPKEWENQDKWNGGWERKSNGDIQPRIGG
KYRILANIFANPDMPEIDDYYEPFDYDYQHLHTAPIVEHQPTARPRSVISGKRMEKIEWGPNWEEILGTEFEKRSKDKNF
DNMQKDMYGEFENTFMMYLPRLCEHCLNPACVASCPSGAIYKREEDGIVLIDQEKCRGWRMCISGCPYKKIYYNWKSGKS
EKCIFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDADKIKEVASTASEQDLYEKQLEVFLDPFDPAVIEQARKDGVADSV
IQAAQKSPVYKMAMDWKLALPLHPEYRTLPMVWYIPSLSPIQNAAESGTLGKAGVIPDVRSLRIPVKYLANLLTAGDEAP
VITALERMLAMRAYKRAENVEGVEDLEVLQQVGLTKKQADEMYRYLAIANYEDRYVIPSGHREMNVPEAYGEKSGCGFTF
GNGCSDGHNGVNMFGGKKITRRNIIKTVSVED

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=500, Percent_Identity=75.2, Blast_Score=810, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=508, Percent_Identity=71.8503937007874, Blast_Score=802, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=131, Percent_Identity=35.8778625954198, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787122, Length=131, Percent_Identity=35.8778625954198, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI2367345, Length=135, Percent_Identity=28.1481481481481, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI226510944, Length=120, Percent_Identity=30, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789370, Length=91, Percent_Identity=37.3626373626374, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58303; Mature: 58303

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQIGMVLNLDKCIGCHTCSITCKNVWTSREGVEYAWFNNVETKPGIGYPKEWENQD
CCCHHHCCCEEEHHHHCCCEEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWNGGWERKSNGDIQPRIGGKYRILANIFANPDMPEIDDYYEPFDYDYQHLHTAPIVEHQ
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCCCCCHHHHCCCCCCCCC
PTARPRSVISGKRMEKIEWGPNWEEILGTEFEKRSKDKNFDNMQKDMYGEFENTFMMYLP
CCCCCHHHHCCCCHHCCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGAIYKREEDGIVLIDQEKCRGWRMCISGCPYKKIYYNWKSGKS
HHHHHHCCCHHHHCCCCCCCEEECCCCEEEEEHHHCCHHHHHHCCCCCHHEEEEECCCCC
EKCIFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDADKIKEVASTASEQDLYEKQLEVFL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHHCCHHHHHHHHHHHHHC
DPFDPAVIEQARKDGVADSVIQAAQKSPVYKMAMDWKLALPLHPEYRTLPMVWYIPSLSP
CCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCEEEEECCCCCH
IQNAAESGTLGKAGVIPDVRSLRIPVKYLANLLTAGDEAPVITALERMLAMRAYKRAENV
HHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
EGVEDLEVLQQVGLTKKQADEMYRYLAIANYEDRYVIPSGHREMNVPEAYGEKSGCGFTF
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHCCCCCCCCCC
GNGCSDGHNGVNMFGGKKITRRNIIKTVSVED
CCCCCCCCCCCCCCCCCEEHHHHHEEEEECCC
>Mature Secondary Structure
MKIRSQIGMVLNLDKCIGCHTCSITCKNVWTSREGVEYAWFNNVETKPGIGYPKEWENQD
CCCHHHCCCEEEHHHHCCCEEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWNGGWERKSNGDIQPRIGGKYRILANIFANPDMPEIDDYYEPFDYDYQHLHTAPIVEHQ
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCCCCCHHHHCCCCCCCCC
PTARPRSVISGKRMEKIEWGPNWEEILGTEFEKRSKDKNFDNMQKDMYGEFENTFMMYLP
CCCCCHHHHCCCCHHCCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGAIYKREEDGIVLIDQEKCRGWRMCISGCPYKKIYYNWKSGKS
HHHHHHCCCHHHHCCCCCCCEEECCCCEEEEEHHHCCHHHHHHCCCCCHHEEEEECCCCC
EKCIFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDADKIKEVASTASEQDLYEKQLEVFL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHHCCHHHHHHHHHHHHHC
DPFDPAVIEQARKDGVADSVIQAAQKSPVYKMAMDWKLALPLHPEYRTLPMVWYIPSLSP
CCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCEEEEECCCCCH
IQNAAESGTLGKAGVIPDVRSLRIPVKYLANLLTAGDEAPVITALERMLAMRAYKRAENV
HHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
EGVEDLEVLQQVGLTKKQADEMYRYLAIANYEDRYVIPSGHREMNVPEAYGEKSGCGFTF
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHCCCCCCCCCC
GNGCSDGHNGVNMFGGKKITRRNIIKTVSVED
CCCCCCCCCCCCCCCCCEEHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]