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The map label for this gene is fadA [H]

Identifier: 167621956

GI number: 167621956

Start: 17651

End: 18814

Strand: Reverse

Name: fadA [H]

Synonym: Shal_0015

Alternate gene names: 167621956

Gene position: 18814-17651 (Counterclockwise)

Preceding gene: 167621957

Following gene: 167621955

Centisome position: 0.36

GC content: 50.69

Gene sequence:

>1164_bases
ATGAAAAATGCCGTTATCGTAGATTGCATTCGTACTCCAATGGGCCGCTCAAAGGCTGGAGTATTTAGAAATGTGCGTGC
AGAAACCCTCTCTGCAGAACTAATGAAGTCACTACTTGAGCGCAACCCAAAGCTTGACCCAAACACCATTGAAGATGTGA
TGTGGGGTTGTGTGCAGCAAACCCTTGAGCAAGGCTTTAATATTGCCCGCAACGCCGCCCTACTCGCTGGCATTCCAAAA
CAAGTCGGTGCTGTTACCGTAAACCGTTTATGTGGTTCATCGATGGATGCACTGCATCAAGCAGCGCGCGCCATTATGAC
AGGCCAAGGCGATACCTTTATCGTAGGTGGTGTTGAACATATGGGTCATGTACCAATGAACCATGGCGTCGACTTTCACC
CAGGTCTTGCCAACAACGTAGCGAAGGGCTCTGCCATGATGGGTCTGACCGCAGAGATGCTAGGTAAGATGCACGGCATT
ACCCGTGAGCAACAAGACGCTTTTGCCGTACGTTCACATCAACGCGCTCATGCTGCAACGGTTGAAGGCCGTTTCGCTAA
CGAGATTGTGGCTATTGAAGGTCACGATGCCGATGGCGCCCTGATTAGAGTAGACCATGATGAAGTGATCCGCCCTGAAA
CATCGATGGAGTCACTATCAGGTTTACGCCCAGCTTTTGACCCAGCCAACGGCACGGTGACAGCGGGTACCTCATCTGCA
CTTTCTGATGGCGCTTCAGCCATGCTAGTGATGGAAGAAGAGAAAGCCAAAGCGCTAGGCCTACCAATTCGCGCACGTAT
TCGTTCTATGGCGATTGCAGGTTGTGATGCCGCTATTATGGGTTACGGCCCAGTACCCGCCACTCAAAAAGCCCTTAAGC
GTGCAGGCCTCACCGTTGATGACCTAGACGTTATCGAGCTTAACGAAGCTTTTGCCGCGCAGTCTCTACCCTGTGTAAAA
GACTTAGGCTTGATGGATGTAGTCGATGAGAAGGTCAACCTTAACGGTGGTGCGATTGCACTGGGTCATCCACTAGGTTG
CTCGGGTACGCGTATCTCAACCACGCTTATCAACCTGATGGAAGCTAAAGATGCTAAGTATGGTCTTGCGACCATGTGTA
TTGGTCTAGGCCAAGGTATTGCAACTATCTTCGAGCGTCCATAA

Upstream 100 bases:

>100_bases
ACACTTAGGTGGTCTATACCAAGTAACCGACACTATGCGTGAACTTGCCGCCAACAATGGCAGCTACTACCAGCAGGCTT
AAGCAGGAAGGATTAGAACA

Downstream 100 bases:

>100_bases
GCATTCGCTCCCGAGTAAACAAAAGCCAGCGCAAGCTGGCTTTTTTGTTTTCAGAATCGGCCTAATTTTCCAAAATAGAA
AGCATTAATAACTCAATATC

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKNAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKSLLERNPKLDPNTIEDVMWGCVQQTLEQGFNIARNAALLAGIPK
QVGAVTVNRLCGSSMDALHQAARAIMTGQGDTFIVGGVEHMGHVPMNHGVDFHPGLANNVAKGSAMMGLTAEMLGKMHGI
TREQQDAFAVRSHQRAHAATVEGRFANEIVAIEGHDADGALIRVDHDEVIRPETSMESLSGLRPAFDPANGTVTAGTSSA
LSDGASAMLVMEEEKAKALGLPIRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGLTVDDLDVIELNEAFAAQSLPCVK
DLGLMDVVDEKVNLNGGAIALGHPLGCSGTRISTTLINLMEAKDAKYGLATMCIGLGQGIATIFERP

Sequences:

>Translated_387_residues
MKNAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKSLLERNPKLDPNTIEDVMWGCVQQTLEQGFNIARNAALLAGIPK
QVGAVTVNRLCGSSMDALHQAARAIMTGQGDTFIVGGVEHMGHVPMNHGVDFHPGLANNVAKGSAMMGLTAEMLGKMHGI
TREQQDAFAVRSHQRAHAATVEGRFANEIVAIEGHDADGALIRVDHDEVIRPETSMESLSGLRPAFDPANGTVTAGTSSA
LSDGASAMLVMEEEKAKALGLPIRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGLTVDDLDVIELNEAFAAQSLPCVK
DLGLMDVVDEKVNLNGGAIALGHPLGCSGTRISTTLINLMEAKDAKYGLATMCIGLGQGIATIFERP
>Mature_387_residues
MKNAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKSLLERNPKLDPNTIEDVMWGCVQQTLEQGFNIARNAALLAGIPK
QVGAVTVNRLCGSSMDALHQAARAIMTGQGDTFIVGGVEHMGHVPMNHGVDFHPGLANNVAKGSAMMGLTAEMLGKMHGI
TREQQDAFAVRSHQRAHAATVEGRFANEIVAIEGHDADGALIRVDHDEVIRPETSMESLSGLRPAFDPANGTVTAGTSSA
LSDGASAMLVMEEEKAKALGLPIRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGLTVDDLDVIELNEAFAAQSLPCVK
DLGLMDVVDEKVNLNGGAIALGHPLGCSGTRISTTLINLMEAKDAKYGLATMCIGLGQGIATIFERP

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=37.6884422110553, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI4501853, Length=394, Percent_Identity=40.6091370558376, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI148539872, Length=395, Percent_Identity=37.9746835443038, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4504327, Length=434, Percent_Identity=33.1797235023041, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI4557237, Length=399, Percent_Identity=33.5839598997494, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI194353979, Length=94, Percent_Identity=53.1914893617021, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI48994986, Length=386, Percent_Identity=72.5388601036269, Blast_Score=586, Evalue=1e-169,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=46.9135802469136, Blast_Score=337, Evalue=9e-94,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=42.3940149625935, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1788554, Length=400, Percent_Identity=44.75, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=33.8199513381995, Blast_Score=184, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI133906874, Length=400, Percent_Identity=37.75, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17535921, Length=402, Percent_Identity=35.5721393034826, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=433, Percent_Identity=32.5635103926097, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI25147385, Length=397, Percent_Identity=33.5012594458438, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17535917, Length=383, Percent_Identity=29.5039164490862, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6322031, Length=395, Percent_Identity=38.7341772151899, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6325229, Length=404, Percent_Identity=34.9009900990099, Blast_Score=192, Evalue=7e-50,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=38.25, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17648125, Length=334, Percent_Identity=39.8203592814371, Blast_Score=217, Evalue=9e-57,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=34.4186046511628, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24640423, Length=395, Percent_Identity=32.6582278481013, Blast_Score=178, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40814; Mature: 40814

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKSLLERNPKLDPNTIEDVMWGCVQQ
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TLEQGFNIARNAALLAGIPKQVGAVTVNRLCGSSMDALHQAARAIMTGQGDTFIVGGVEH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECHHH
MGHVPMNHGVDFHPGLANNVAKGSAMMGLTAEMLGKMHGITREQQDAFAVRSHQRAHAAT
HCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHE
VEGRFANEIVAIEGHDADGALIRVDHDEVIRPETSMESLSGLRPAFDPANGTVTAGTSSA
ECCCCCCEEEEEECCCCCCEEEEECCHHHCCCCCHHHHHHCCCCCCCCCCCEEECCCCHH
LSDGASAMLVMEEEKAKALGLPIRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGLTVD
HCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCC
DLDVIELNEAFAAQSLPCVKDLGLMDVVDEKVNLNGGAIALGHPLGCSGTRISTTLINLM
CCCEEECCHHHHHCCCCHHHHCCHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
EAKDAKYGLATMCIGLGQGIATIFERP
HHCCCCHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MKNAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKSLLERNPKLDPNTIEDVMWGCVQQ
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TLEQGFNIARNAALLAGIPKQVGAVTVNRLCGSSMDALHQAARAIMTGQGDTFIVGGVEH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECHHH
MGHVPMNHGVDFHPGLANNVAKGSAMMGLTAEMLGKMHGITREQQDAFAVRSHQRAHAAT
HCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHE
VEGRFANEIVAIEGHDADGALIRVDHDEVIRPETSMESLSGLRPAFDPANGTVTAGTSSA
ECCCCCCEEEEEECCCCCCEEEEECCHHHCCCCCHHHHHHCCCCCCCCCCCEEECCCCHH
LSDGASAMLVMEEEKAKALGLPIRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGLTVD
HCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCC
DLDVIELNEAFAAQSLPCVKDLGLMDVVDEKVNLNGGAIALGHPLGCSGTRISTTLINLM
CCCEEECCHHHHHCCCCHHHHCCHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
EAKDAKYGLATMCIGLGQGIATIFERP
HHCCCCHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA