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The map label for this gene is grpE [H]

Identifier: 167040703

GI number: 167040703

Start: 2091417

End: 2092004

Strand: Reverse

Name: grpE [H]

Synonym: Teth514_2080

Alternate gene names: 167040703

Gene position: 2092004-2091417 (Counterclockwise)

Preceding gene: 167040704

Following gene: 167040702

Centisome position: 85.14

GC content: 33.5

Gene sequence:

>588_bases
GTGGAAGAAGTAGAAAAGGAAATTAACAAAGAGGAGGAAAAGGATGTTAATAATTTAGATTCAAACGAACAAATGGAAGG
ACCTCCCGAAGATGAACAAGCACAGCAACAGCCACAGCAGCAAACGGTAGATGAAATAGAAGAACTAAAGCAAAAACTTC
AGCAAAAAGAAGTTGAAGCTCAAGAATATTTAGATATTGCACAAAGGTTGAAAGCAGAATTTGAAAATTACAGAAAGAGA
ACAGAAAAAGAAAAATCAGAAATGGTAGAATACGGGAAAGAAACAGTAATTTTAGAACTTTTGCCTATTATGGATAATTT
TGAAAGAGCTTTGGCGAGCAGTGGAGATTATAATTCTCTAAAAGAAGGGATAGAATTAATTTACAGGCAGTTTAAAAAGA
TATTGGATAAATTTGGCGTGAAAGAAATTGAAGCAGAAGGGCAAATTTTTGACCCCTACAAACACCATGCTGTAATGCAG
GAAGAGGTGGAAGGAAAACAGCCCAATGAAATTATTGAAGTTTTCCAAAAAGGCTATTATCTAAAAGATAAAGTAATAAG
ACCAAGTTTAGTAAAAGTAGCAAAATAA

Upstream 100 bases:

>100_bases
GATTATAGAAAATTAATTAATGAAATCAATGTTATGACAAAAGAGCTTTCGAATCTTTTATCGAATATATACAATGATGA
AATATAATGAGGTGATATAA

Downstream 100 bases:

>100_bases
AAAGGAGGTTGAAAAAGATGGGGAAAGTTATAGGAATTGACTTAGGAACTACTTTTTCTTGTGTTGCTGTTATGGAAGGA
GGCCAACCTGTAGTAATACC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MEEVEKEINKEEEKDVNNLDSNEQMEGPPEDEQAQQQPQQQTVDEIEELKQKLQQKEVEAQEYLDIAQRLKAEFENYRKR
TEKEKSEMVEYGKETVILELLPIMDNFERALASSGDYNSLKEGIELIYRQFKKILDKFGVKEIEAEGQIFDPYKHHAVMQ
EEVEGKQPNEIIEVFQKGYYLKDKVIRPSLVKVAK

Sequences:

>Translated_195_residues
MEEVEKEINKEEEKDVNNLDSNEQMEGPPEDEQAQQQPQQQTVDEIEELKQKLQQKEVEAQEYLDIAQRLKAEFENYRKR
TEKEKSEMVEYGKETVILELLPIMDNFERALASSGDYNSLKEGIELIYRQFKKILDKFGVKEIEAEGQIFDPYKHHAVMQ
EEVEGKQPNEIIEVFQKGYYLKDKVIRPSLVKVAK
>Mature_195_residues
MEEVEKEINKEEEKDVNNLDSNEQMEGPPEDEQAQQQPQQQTVDEIEELKQKLQQKEVEAQEYLDIAQRLKAEFENYRKR
TEKEKSEMVEYGKETVILELLPIMDNFERALASSGDYNSLKEGIELIYRQFKKILDKFGVKEIEAEGQIFDPYKHHAVMQ
EEVEGKQPNEIIEVFQKGYYLKDKVIRPSLVKVAK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=137, Percent_Identity=32.1167883211679, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1788967, Length=182, Percent_Identity=33.5164835164835, Blast_Score=94, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324806, Length=159, Percent_Identity=30.8176100628931, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24653432, Length=172, Percent_Identity=29.0697674418605, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22986; Mature: 22986

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEVEKEINKEEEKDVNNLDSNEQMEGPPEDEQAQQQPQQQTVDEIEELKQKLQQKEVEA
CCHHHHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
QEYLDIAQRLKAEFENYRKRTEKEKSEMVEYGKETVILELLPIMDNFERALASSGDYNSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KEGIELIYRQFKKILDKFGVKEIEAEGQIFDPYKHHAVMQEEVEGKQPNEIIEVFQKGYY
HHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCH
LKDKVIRPSLVKVAK
HHHHHCCHHHHHHCC
>Mature Secondary Structure
MEEVEKEINKEEEKDVNNLDSNEQMEGPPEDEQAQQQPQQQTVDEIEELKQKLQQKEVEA
CCHHHHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
QEYLDIAQRLKAEFENYRKRTEKEKSEMVEYGKETVILELLPIMDNFERALASSGDYNSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KEGIELIYRQFKKILDKFGVKEIEAEGQIFDPYKHHAVMQEEVEGKQPNEIIEVFQKGYY
HHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCH
LKDKVIRPSLVKVAK
HHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]