Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 167040684

GI number: 167040684

Start: 2074018

End: 2074974

Strand: Reverse

Name: phoB [H]

Synonym: Teth514_2061

Alternate gene names: 167040684

Gene position: 2074974-2074018 (Counterclockwise)

Preceding gene: 167040685

Following gene: 167040683

Centisome position: 84.44

GC content: 31.66

Gene sequence:

>957_bases
ATGTTTTTTTGTGGAGGAAGCATTGTGAAGGGGAGAAACATTCTTATTGTCGATGATAACAAACTTACAAGGAAAATTTT
GAAAGATATTTTAGAAAATGCTGGGTATGGGGTGCTAGAGGCTAAAGATGGAGAAGAGGCTTTACAAATATACAAGAATA
AACTACCTGATATGGTAATATTAGATATAGTTATGCCAGGCATGACTGGCTTTGACCTTTTAAGAATTTTGAGGAAAGAA
GAAGAAAATTTGATGCTTCCTATAATACTTTTGACTTCACAGGACAATTTTGAAGAAAAAATTAAAGGGTTGGAATTGGG
TGCAGATGATTATTTAGTGAAACCTTTTAATGACAAAGAACTTCTGTTTAAAGTCAATAATTTGTTTCAGCGGATAGAAC
ACAACAGAATGGCCAACCCTCTTACTGGTCTTAGAGGAAATATTGACATTAAAGAAGAAATAAAAAGGCGAATTAAAGAA
AACAAGCCTTATGCCATTTTGTATATTGATTTGGACAATTTTAAGTCTTATAATGATTACTATGGTTTTCTAAGAGGAGA
TAATGTAATAAAACTTACAGCCAAAATTTTATCAGATGCTGTAAATCTGATTGGAAATGAAAGAGATTTTTTGGGACACA
TAGGTGGAGATGATTTTGTGATAATTACTACTCCTGATAAAGCTGAAGAGATGTGTAAGTATATAATTTCTCGCTTTGAT
GAGGAAATAAAGTATTTATATGACGATGAAGATAGAAAACGGGGTTACATAGAGACAATTGGTAGGACTGGCGAAAAGAT
GATGTTTCCACTGGTATCAATTTCTATTGCTATTGTCACAAATGAATTTAGAGATTTTAAAAATGATTTGGAAATAAGTG
CAGTAGCTGCTGAATTGAAAAAGAAATTAAAGACAATGGATGGAAGTTGTTATTTAAAAGATAGAAGAAAAGGTTAA

Upstream 100 bases:

>100_bases
AAAATTTTATGTAAAATTTTTTCATTTTTAATTTAAATTTAATTTGCTAAAGTAGTAGTTTTTTTGCCAAAATTTTTATA
TAATATTATGGGGTTGTTAA

Downstream 100 bases:

>100_bases
AGAAAAGGTTAAGGGCATACAACAGTATGCCCTATCTTTGTATAAAAGAGGTGAAAAAATGTCTAATCCCTTTGTTCCCC
ATGGCAATTTTAAATGGGTT

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MFFCGGSIVKGRNILIVDDNKLTRKILKDILENAGYGVLEAKDGEEALQIYKNKLPDMVILDIVMPGMTGFDLLRILRKE
EENLMLPIILLTSQDNFEEKIKGLELGADDYLVKPFNDKELLFKVNNLFQRIEHNRMANPLTGLRGNIDIKEEIKRRIKE
NKPYAILYIDLDNFKSYNDYYGFLRGDNVIKLTAKILSDAVNLIGNERDFLGHIGGDDFVIITTPDKAEEMCKYIISRFD
EEIKYLYDDEDRKRGYIETIGRTGEKMMFPLVSISIAIVTNEFRDFKNDLEISAVAAELKKKLKTMDGSCYLKDRRKG

Sequences:

>Translated_318_residues
MFFCGGSIVKGRNILIVDDNKLTRKILKDILENAGYGVLEAKDGEEALQIYKNKLPDMVILDIVMPGMTGFDLLRILRKE
EENLMLPIILLTSQDNFEEKIKGLELGADDYLVKPFNDKELLFKVNNLFQRIEHNRMANPLTGLRGNIDIKEEIKRRIKE
NKPYAILYIDLDNFKSYNDYYGFLRGDNVIKLTAKILSDAVNLIGNERDFLGHIGGDDFVIITTPDKAEEMCKYIISRFD
EEIKYLYDDEDRKRGYIETIGRTGEKMMFPLVSISIAIVTNEFRDFKNDLEISAVAAELKKKLKTMDGSCYLKDRRKG
>Mature_318_residues
MFFCGGSIVKGRNILIVDDNKLTRKILKDILENAGYGVLEAKDGEEALQIYKNKLPDMVILDIVMPGMTGFDLLRILRKE
EENLMLPIILLTSQDNFEEKIKGLELGADDYLVKPFNDKELLFKVNNLFQRIEHNRMANPLTGLRGNIDIKEEIKRRIKE
NKPYAILYIDLDNFKSYNDYYGFLRGDNVIKLTAKILSDAVNLIGNERDFLGHIGGDDFVIITTPDKAEEMCKYIISRFD
EEIKYLYDDEDRKRGYIETIGRTGEKMMFPLVSISIAIVTNEFRDFKNDLEISAVAAELKKKLKTMDGSCYLKDRRKG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790863, Length=119, Percent_Identity=48.7394957983193, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=34.4262295081967, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082012, Length=134, Percent_Identity=36.5671641791045, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=43.2203389830509, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787375, Length=118, Percent_Identity=35.5932203389831, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787229, Length=130, Percent_Identity=37.6923076923077, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI2367329, Length=118, Percent_Identity=41.5254237288136, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788394, Length=124, Percent_Identity=31.4516129032258, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789809, Length=130, Percent_Identity=33.8461538461538, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786911, Length=130, Percent_Identity=33.8461538461538, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790860, Length=154, Percent_Identity=27.9220779220779, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790299, Length=104, Percent_Identity=34.6153846153846, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789402, Length=121, Percent_Identity=38.8429752066116, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=35.8974358974359, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787487, Length=119, Percent_Identity=32.7731092436975, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=34.453781512605, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790552, Length=125, Percent_Identity=36.8, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693157, Length=102, Percent_Identity=39.2156862745098, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788713, Length=118, Percent_Identity=40.6779661016949, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790437, Length=105, Percent_Identity=32.3809523809524, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 36636; Mature: 36636

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFCGGSIVKGRNILIVDDNKLTRKILKDILENAGYGVLEAKDGEEALQIYKNKLPDMVI
CEEECCCEECCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCEEE
LDIVMPGMTGFDLLRILRKEEENLMLPIILLTSQDNFEEKIKGLELGADDYLVKPFNDKE
EEHHHCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHCCCCCCCCCCEECCCCCHH
LLFKVNNLFQRIEHNRMANPLTGLRGNIDIKEEIKRRIKENKPYAILYIDLDNFKSYNDY
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
YGFLRGDNVIKLTAKILSDAVNLIGNERDFLGHIGGDDFVIITTPDKAEEMCKYIISRFD
CCEECCCCEEEHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHH
EEIKYLYDDEDRKRGYIETIGRTGEKMMFPLVSISIAIVTNEFRDFKNDLEISAVAAELK
HHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHH
KKLKTMDGSCYLKDRRKG
HHHHHCCCCEEECCCCCC
>Mature Secondary Structure
MFFCGGSIVKGRNILIVDDNKLTRKILKDILENAGYGVLEAKDGEEALQIYKNKLPDMVI
CEEECCCEECCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCEEE
LDIVMPGMTGFDLLRILRKEEENLMLPIILLTSQDNFEEKIKGLELGADDYLVKPFNDKE
EEHHHCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHCCCCCCCCCCEECCCCCHH
LLFKVNNLFQRIEHNRMANPLTGLRGNIDIKEEIKRRIKENKPYAILYIDLDNFKSYNDY
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
YGFLRGDNVIKLTAKILSDAVNLIGNERDFLGHIGGDDFVIITTPDKAEEMCKYIISRFD
CCEECCCCEEEHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHH
EEIKYLYDDEDRKRGYIETIGRTGEKMMFPLVSISIAIVTNEFRDFKNDLEISAVAAELK
HHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHH
KKLKTMDGSCYLKDRRKG
HHHHHCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]