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The map label for this gene is lipA [H]

Identifier: 167040659

GI number: 167040659

Start: 2046173

End: 2047021

Strand: Reverse

Name: lipA [H]

Synonym: Teth514_2036

Alternate gene names: 167040659

Gene position: 2047021-2046173 (Counterclockwise)

Preceding gene: 167040660

Following gene: 167040658

Centisome position: 83.31

GC content: 36.87

Gene sequence:

>849_bases
ATGCAAAAGCCTGAATGGCTCAAAGTGAGGCTATTAAACGAAGATTTAAACAGGATGGAAGCTTTTTTAAAAAGTATGTC
TTTAAACACAGTTTGTCAAAGTGCCAATTGTCCCAACATGGGGGAATGCTTTGCGAGAAAGACAGCCACTTTTATGATTA
TGGGAAATATATGTACGCGAAACTGTAGGTTTTGTGCGGTAGAAAAAGGACATCCTCAACCTCTTGATGAGGATGAGCCT
CGAAGGATTGCTGAGGCTGCTCAAAAGTTAGGATTAAAGCATGTAGTGGTTACTTGTGTTACAAGAGATGATTTACCGGA
TGGTGGAGCATCACATTTTGCAAAGACAATCTATGAACTTAAAAAATTGCCAGGAGTTACAGTTGAGGTACTTGTATCTG
ATTTTATGGGTAAAGAAGAGTCTATTGCTCAAGTAGTAGAAGCCAAGCCAGATATAATAAATCACAATATAGAAACAGTA
CCGAGACTTTATCCCAATGTAAGGCCAAAAGCAGATTATTTAAGGTCTTTAAACCTTTTAAAAAAGTCAAAAGAATTAGA
CCCTTATATTCTTACCAAGTCAGGAATTATGGTGGGGCTTGGGGAAACAGAAGAAGAGGTTATACAAGTTATGAAAGACT
TAAGAAGTGTTGATTGTGACATGATGACTATAGGACAGTATTTGAGACCTTCAGCAAAACATATAGAAGTAGCAGAATAT
GTTATTCCTCAGCAATTTAAGAGGTATGAAGAGATAGGATATGAATTAGGTTTTAAATACGTAGCTTCTGGTCCCTTAGT
CAGAAGTTCTTATTATGCTGATGAAGGATTGAAGTTGACAAAGGCTTAG

Upstream 100 bases:

>100_bases
GACATAGAAAAAGTAAAAGAAAAAATGGTTGATAAATTTAGCGAAGTATTTGGTATACACTTTAGTGAGATAACTTTAGA
TAGATTGGCGGTGATTGATA

Downstream 100 bases:

>100_bases
TTTTTAAGCCTTTGTTTTTGCATGAGGAGAATTTTAAAACGATTTAAGGGGGAGTGTCATGAAAGATTTTATATCTTTTA
TGGAGAAAGCTTGGTGGAGA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MQKPEWLKVRLLNEDLNRMEAFLKSMSLNTVCQSANCPNMGECFARKTATFMIMGNICTRNCRFCAVEKGHPQPLDEDEP
RRIAEAAQKLGLKHVVVTCVTRDDLPDGGASHFAKTIYELKKLPGVTVEVLVSDFMGKEESIAQVVEAKPDIINHNIETV
PRLYPNVRPKADYLRSLNLLKKSKELDPYILTKSGIMVGLGETEEEVIQVMKDLRSVDCDMMTIGQYLRPSAKHIEVAEY
VIPQQFKRYEEIGYELGFKYVASGPLVRSSYYADEGLKLTKA

Sequences:

>Translated_282_residues
MQKPEWLKVRLLNEDLNRMEAFLKSMSLNTVCQSANCPNMGECFARKTATFMIMGNICTRNCRFCAVEKGHPQPLDEDEP
RRIAEAAQKLGLKHVVVTCVTRDDLPDGGASHFAKTIYELKKLPGVTVEVLVSDFMGKEESIAQVVEAKPDIINHNIETV
PRLYPNVRPKADYLRSLNLLKKSKELDPYILTKSGIMVGLGETEEEVIQVMKDLRSVDCDMMTIGQYLRPSAKHIEVAEY
VIPQQFKRYEEIGYELGFKYVASGPLVRSSYYADEGLKLTKA
>Mature_282_residues
MQKPEWLKVRLLNEDLNRMEAFLKSMSLNTVCQSANCPNMGECFARKTATFMIMGNICTRNCRFCAVEKGHPQPLDEDEP
RRIAEAAQKLGLKHVVVTCVTRDDLPDGGASHFAKTIYELKKLPGVTVEVLVSDFMGKEESIAQVVEAKPDIINHNIETV
PRLYPNVRPKADYLRSLNLLKKSKELDPYILTKSGIMVGLGETEEEVIQVMKDLRSVDCDMMTIGQYLRPSAKHIEVAEY
VIPQQFKRYEEIGYELGFKYVASGPLVRSSYYADEGLKLTKA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=284, Percent_Identity=46.830985915493, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=44.3089430894309, Blast_Score=222, Evalue=3e-58,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=47.841726618705, Blast_Score=276, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=45.6445993031359, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6324770, Length=283, Percent_Identity=45.2296819787986, Blast_Score=260, Evalue=2e-70,
Organism=Drosophila melanogaster, GI221513272, Length=284, Percent_Identity=46.4788732394366, Blast_Score=267, Evalue=7e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 31884; Mature: 31884

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKPEWLKVRLLNEDLNRMEAFLKSMSLNTVCQSANCPNMGECFARKTATFMIMGNICTR
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEECCHHCC
NCRFCAVEKGHPQPLDEDEPRRIAEAAQKLGLKHVVVTCVTRDDLPDGGASHFAKTIYEL
CCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
KKLPGVTVEVLVSDFMGKEESIAQVVEAKPDIINHNIETVPRLYPNVRPKADYLRSLNLL
HHCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCHHHHHHHHHHH
KKSKELDPYILTKSGIMVGLGETEEEVIQVMKDLRSVDCDMMTIGQYLRPSAKHIEVAEY
HHHCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHH
VIPQQFKRYEEIGYELGFKYVASGPLVRSSYYADEGLKLTKA
HHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHCCCCCEEECCC
>Mature Secondary Structure
MQKPEWLKVRLLNEDLNRMEAFLKSMSLNTVCQSANCPNMGECFARKTATFMIMGNICTR
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEECCHHCC
NCRFCAVEKGHPQPLDEDEPRRIAEAAQKLGLKHVVVTCVTRDDLPDGGASHFAKTIYEL
CCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
KKLPGVTVEVLVSDFMGKEESIAQVVEAKPDIINHNIETVPRLYPNVRPKADYLRSLNLL
HHCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCHHHHHHHHHHH
KKSKELDPYILTKSGIMVGLGETEEEVIQVMKDLRSVDCDMMTIGQYLRPSAKHIEVAEY
HHHCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHH
VIPQQFKRYEEIGYELGFKYVASGPLVRSSYYADEGLKLTKA
HHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]