Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 167040637

GI number: 167040637

Start: 2022981

End: 2024432

Strand: Reverse

Name: murE [H]

Synonym: Teth514_2014

Alternate gene names: 167040637

Gene position: 2024432-2022981 (Counterclockwise)

Preceding gene: 167040638

Following gene: 167040636

Centisome position: 82.39

GC content: 35.19

Gene sequence:

>1452_bases
ATGAAACTAATAGATATTTTGAAGGGTGTTAAGTGCGAGATAAAAGGAGATCCTAATATAGACATCAGTGGGGTGTGTTA
CGATTCCCGTAAAGCCAAAAAGAAATATTTATTTATTGCTATAAAAGGGTTTAAAACCGATGGTATTTTCTATGTGGAAG
AGGCTATTAAAAATGGGGCAGTTGCAGTGGTAGCAGATAGGGAAATAAAAGAATATCCTGGCATTACAACAGTATTAGTA
GAAGATGCTCGAGCTACCATGGCAAAAATAGCTTCTAATTTTTACAATAACCCCACCAGCAAACTTACTTTAATTGGTGT
AACAGGAACAAATGGCAAAACCTCTGTTACTTATATGCTAAAAGCGATTTTAGAAAGGCAAAATAATAAAGTGGGCCTGG
TGGGGACTATACAAAATATGATAGGGGATGAAGTGATACCTTCTGTACATACCACACCTGAATCGTTAGATTTGCAGGAG
CTTTTTAGCATTATGGTGAACAAAGGTGTTAAGTATGTAGTTATGGAGGTATCGTCCCATTCTTTAGCATTAAATAGAGT
AGACGAATGTGAATTTGATATAGCAATTTTTACCAACTTATCCCAGGACCATTTAGATTTTCACCAGAACATGGAAGAGT
ATGCTAAGACAAAGAGTAAGTTATTCAAGATGGCAAAGAGGGCGTCGGTTATCAATATAGATGATAAATACTCTTCTATG
ATGATTGAGAGTTCTAAAGCAAAGGTTTTGACTTATGGTATAGAAGATTTTGCTTATGTTATGGCAAAGGATATTAGGAA
TAGTTTAGATGGTGTAAAGTTTAAAGTTCAGATTGAAGATAAAAAAGAAGAGATGTCATTGAAAATTCCTGGTTTGTTTA
ATGTCTACAATGCTTTAGCTGCTATTACAGTGGCTGATTTTCTTGGTATTTCTTTGCGAAGTGTGAGAGAGGCTTTAAGT
CAAGTTACAGTAAAAGGTCGATTTGAACCGGTGGAGACAGGAAAAGACTTTTATGTGTTCATAGATTATGCCCATACTCC
TGACGGAATACAGAATATAATGGAAGCCTTGAAGGAATATAAAACTGGGAGAAAAATACTTCTTTTTGGTGCTGGAGGAG
ATAGGGATAAAACTAAAAGGCCTTTGATGGGAGAAGTTGCAGGAAAATATGCAGATTTTTGCATTTTGACATCAGACAAT
CCTCGTTCGGAGGACCCAAGAGAAATAATTTCACAAATTGAGGAAGGCATAAAAAAGACTAATTGCCCTTATGTTGTAAT
AGAAAATAGAAAAGAAGCGATAAGATACGCTCTGACTAATGCTCAAAAAGACGATGTAATAATATTAGCAGGCAAAGGCC
ACGAAACTTATCAAATTATAGGAAATAAGGTTATTCCCTTTGATGAGAGAGAAATAGTTAAAGAAATTTTGTCTGAAGGT
GGTAATAAATGA

Upstream 100 bases:

>100_bases
GGCCTAAGGAAAAATAAACTTCCTCTATGCAAAATCTATAATTGTGCTATACTATTTAGTGGTGGTATGCTTTTTAGATA
TTTTTGTGAGGAGGCCTAAC

Downstream 100 bases:

>100_bases
TGGAACTTTTAATAGATGAAATTGTAAGAGCCACAGAGGGAAAATTGATAAAAGGAAATTTAAAGGACAAAGTAATGGGA
ATTTCGACTGATTCGAGAAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKLIDILKGVKCEIKGDPNIDISGVCYDSRKAKKKYLFIAIKGFKTDGIFYVEEAIKNGAVAVVADREIKEYPGITTVLV
EDARATMAKIASNFYNNPTSKLTLIGVTGTNGKTSVTYMLKAILERQNNKVGLVGTIQNMIGDEVIPSVHTTPESLDLQE
LFSIMVNKGVKYVVMEVSSHSLALNRVDECEFDIAIFTNLSQDHLDFHQNMEEYAKTKSKLFKMAKRASVINIDDKYSSM
MIESSKAKVLTYGIEDFAYVMAKDIRNSLDGVKFKVQIEDKKEEMSLKIPGLFNVYNALAAITVADFLGISLRSVREALS
QVTVKGRFEPVETGKDFYVFIDYAHTPDGIQNIMEALKEYKTGRKILLFGAGGDRDKTKRPLMGEVAGKYADFCILTSDN
PRSEDPREIISQIEEGIKKTNCPYVVIENRKEAIRYALTNAQKDDVIILAGKGHETYQIIGNKVIPFDEREIVKEILSEG
GNK

Sequences:

>Translated_483_residues
MKLIDILKGVKCEIKGDPNIDISGVCYDSRKAKKKYLFIAIKGFKTDGIFYVEEAIKNGAVAVVADREIKEYPGITTVLV
EDARATMAKIASNFYNNPTSKLTLIGVTGTNGKTSVTYMLKAILERQNNKVGLVGTIQNMIGDEVIPSVHTTPESLDLQE
LFSIMVNKGVKYVVMEVSSHSLALNRVDECEFDIAIFTNLSQDHLDFHQNMEEYAKTKSKLFKMAKRASVINIDDKYSSM
MIESSKAKVLTYGIEDFAYVMAKDIRNSLDGVKFKVQIEDKKEEMSLKIPGLFNVYNALAAITVADFLGISLRSVREALS
QVTVKGRFEPVETGKDFYVFIDYAHTPDGIQNIMEALKEYKTGRKILLFGAGGDRDKTKRPLMGEVAGKYADFCILTSDN
PRSEDPREIISQIEEGIKKTNCPYVVIENRKEAIRYALTNAQKDDVIILAGKGHETYQIIGNKVIPFDEREIVKEILSEG
GNK
>Mature_483_residues
MKLIDILKGVKCEIKGDPNIDISGVCYDSRKAKKKYLFIAIKGFKTDGIFYVEEAIKNGAVAVVADREIKEYPGITTVLV
EDARATMAKIASNFYNNPTSKLTLIGVTGTNGKTSVTYMLKAILERQNNKVGLVGTIQNMIGDEVIPSVHTTPESLDLQE
LFSIMVNKGVKYVVMEVSSHSLALNRVDECEFDIAIFTNLSQDHLDFHQNMEEYAKTKSKLFKMAKRASVINIDDKYSSM
MIESSKAKVLTYGIEDFAYVMAKDIRNSLDGVKFKVQIEDKKEEMSLKIPGLFNVYNALAAITVADFLGISLRSVREALS
QVTVKGRFEPVETGKDFYVFIDYAHTPDGIQNIMEALKEYKTGRKILLFGAGGDRDKTKRPLMGEVAGKYADFCILTSDN
PRSEDPREIISQIEEGIKKTNCPYVVIENRKEAIRYALTNAQKDDVIILAGKGHETYQIIGNKVIPFDEREIVKEILSEG
GNK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=476, Percent_Identity=33.8235294117647, Blast_Score=258, Evalue=9e-70,
Organism=Escherichia coli, GI1786274, Length=389, Percent_Identity=25.1928020565553, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53966; Mature: 53966

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIDILKGVKCEIKGDPNIDISGVCYDSRKAKKKYLFIAIKGFKTDGIFYVEEAIKNGA
CCHHHHHCCCEEEECCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEHHHHCCC
VAVVADREIKEYPGITTVLVEDARATMAKIASNFYNNPTSKLTLIGVTGTNGKTSVTYML
EEEEECCHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHH
KAILERQNNKVGLVGTIQNMIGDEVIPSVHTTPESLDLQELFSIMVNKGVKYVVMEVSSH
HHHHHHCCCCEEEEEEHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCC
SLALNRVDECEFDIAIFTNLSQDHLDFHQNMEEYAKTKSKLFKMAKRASVINIDDKYSSM
EEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHH
MIESSKAKVLTYGIEDFAYVMAKDIRNSLDGVKFKVQIEDKKEEMSLKIPGLFNVYNALA
EEECCCCEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHH
AITVADFLGISLRSVREALSQVTVKGRFEPVETGKDFYVFIDYAHTPDGIQNIMEALKEY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEEEECCCCHHHHHHHHHHHHH
KTGRKILLFGAGGDRDKTKRPLMGEVAGKYADFCILTSDNPRSEDPREIISQIEEGIKKT
CCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHC
NCPYVVIENRKEAIRYALTNAQKDDVIILAGKGHETYQIIGNKVIPFDEREIVKEILSEG
CCCEEEECCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCC
GNK
CCC
>Mature Secondary Structure
MKLIDILKGVKCEIKGDPNIDISGVCYDSRKAKKKYLFIAIKGFKTDGIFYVEEAIKNGA
CCHHHHHCCCEEEECCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEHHHHCCC
VAVVADREIKEYPGITTVLVEDARATMAKIASNFYNNPTSKLTLIGVTGTNGKTSVTYML
EEEEECCHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHH
KAILERQNNKVGLVGTIQNMIGDEVIPSVHTTPESLDLQELFSIMVNKGVKYVVMEVSSH
HHHHHHCCCCEEEEEEHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCC
SLALNRVDECEFDIAIFTNLSQDHLDFHQNMEEYAKTKSKLFKMAKRASVINIDDKYSSM
EEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHH
MIESSKAKVLTYGIEDFAYVMAKDIRNSLDGVKFKVQIEDKKEEMSLKIPGLFNVYNALA
EEECCCCEEEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHH
AITVADFLGISLRSVREALSQVTVKGRFEPVETGKDFYVFIDYAHTPDGIQNIMEALKEY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEEEECCCCHHHHHHHHHHHHH
KTGRKILLFGAGGDRDKTKRPLMGEVAGKYADFCILTSDNPRSEDPREIISQIEEGIKKT
CCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHC
NCPYVVIENRKEAIRYALTNAQKDDVIILAGKGHETYQIIGNKVIPFDEREIVKEILSEG
CCCEEEECCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCC
GNK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]