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The map label for this gene is murF [H]

Identifier: 167040636

GI number: 167040636

Start: 2021599

End: 2022981

Strand: Reverse

Name: murF [H]

Synonym: Teth514_2013

Alternate gene names: 167040636

Gene position: 2022981-2021599 (Counterclockwise)

Preceding gene: 167040637

Following gene: 167040635

Centisome position: 82.33

GC content: 30.73

Gene sequence:

>1383_bases
ATGGAACTTTTAATAGATGAAATTGTAAGAGCCACAGAGGGAAAATTGATAAAAGGAAATTTAAAGGACAAAGTAATGGG
AATTTCGACTGATTCGAGAAAAGTCAAAAAAGATGAATTATTTATCCCTTTGAAAGGGGAAAAATACGATGGAGAAGATT
TCGTAAAAGATGCAATAGACAAAGGTGCTAGTGCAGTTATTACTGCGAGGTTGGATAATGTAGAATGTTTGAAAGATTTT
TCAGTTAATGTCATTTACGTTAATGAGACTTTAGAAGCTTTGCATAAAATTGCTTCTTATTATAGAAAAAAATTCAATAT
TCCCTTTATTGCTGTGACAGGTAGCAGTGGAAAAACTACTACTAAAGATATGATTGCACAAATATTGTCTAAAAAGTATA
AAGTTTTGAAAACAGAGGGAAATTTTAATAATGAAATTGGCTTGCCATTGACAATTTTTAATTTGGAAGATACTCATCAG
ATTGCGGTAGTGGAAATGGGAATGAATGGTTTTGGAGAAATTAGGAGGTTAAAGAATATTGCAGAGCCTCAAGTAGCAGT
TTTTACAAATATAGGAGTTGCTCATATCGAAAAATTAGGCTCAAGAGAAAATATATTAAAAGCAAAAACTGAGTTAATTG
AAAATTTTAAAGAGGAAAATGTGATTGTATTAAATGCAGATGATGATATGCTATCAAAAATTCCTCTGCAGTATTCAAGT
AAGTATTACAGATATGGTATAAAAAGTGGAGAAATTAAAGCTTGCGATATAGAAAAAGAAGAAAAAGATATAAAATATAC
TTTAAAATATAAAAATTTGGCGAAAGAAATAAAGCTTTCGGTTCCTGGTATTCACAATGTGTACAATTCTCTTGCTGCAA
TTTGTGTTGGATTAGAGTTTGGTATTAATGTAGAGGACATGGCAGAGGCTTTAAAAGATTTTCAACCAGGCAAAATGAGA
CTTAATATAATAAAAATAGGTGATATAAAAATTATTGACGATGTATATAATGCTAATCCGGATTCTATGAAAGCAGCTTT
AAGCGTATTAAGGGATTTACCTGCTAAGAGAAAAATAGCAGTTTTAGGAGATATGCTGGAATTAGGTGAGTATGCAGTAG
AAGCCCACAAAGAAATAGGAAAATTGGTGGCAGATAGCCATATAGATATTTTGATAACCTCTGGTGATATGTCGAAATAT
ATTGCAGAAGAATCCAAGGTTTGTGGGATGAAAGAGAATAATATTTTTGTTTGTAACACTAATAAAGAGGTTAATGAAAT
TTTGAAAGATATTATCAAAGAAGGGGATTCAATTTTAGTAAAAGGTTCAAGAGGTATGAAAATGGAACAAATAGTACAAT
TTTTACAGGAGAGGGTAAAGTAA

Upstream 100 bases:

>100_bases
GCAAAGGCCACGAAACTTATCAAATTATAGGAAATAAGGTTATTCCCTTTGATGAGAGAGAAATAGTTAAAGAAATTTTG
TCTGAAGGTGGTAATAAATG

Downstream 100 bases:

>100_bases
AATGTTACAAAAGATAATTTTGGCTACAGTTGTTGCTTTTGTGCTAAGCTTGGCCTCTGGTCCTCTTTTTATACCTTATC
TTAGAAAATTAAAATTTGGG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MELLIDEIVRATEGKLIKGNLKDKVMGISTDSRKVKKDELFIPLKGEKYDGEDFVKDAIDKGASAVITARLDNVECLKDF
SVNVIYVNETLEALHKIASYYRKKFNIPFIAVTGSSGKTTTKDMIAQILSKKYKVLKTEGNFNNEIGLPLTIFNLEDTHQ
IAVVEMGMNGFGEIRRLKNIAEPQVAVFTNIGVAHIEKLGSRENILKAKTELIENFKEENVIVLNADDDMLSKIPLQYSS
KYYRYGIKSGEIKACDIEKEEKDIKYTLKYKNLAKEIKLSVPGIHNVYNSLAAICVGLEFGINVEDMAEALKDFQPGKMR
LNIIKIGDIKIIDDVYNANPDSMKAALSVLRDLPAKRKIAVLGDMLELGEYAVEAHKEIGKLVADSHIDILITSGDMSKY
IAEESKVCGMKENNIFVCNTNKEVNEILKDIIKEGDSILVKGSRGMKMEQIVQFLQERVK

Sequences:

>Translated_460_residues
MELLIDEIVRATEGKLIKGNLKDKVMGISTDSRKVKKDELFIPLKGEKYDGEDFVKDAIDKGASAVITARLDNVECLKDF
SVNVIYVNETLEALHKIASYYRKKFNIPFIAVTGSSGKTTTKDMIAQILSKKYKVLKTEGNFNNEIGLPLTIFNLEDTHQ
IAVVEMGMNGFGEIRRLKNIAEPQVAVFTNIGVAHIEKLGSRENILKAKTELIENFKEENVIVLNADDDMLSKIPLQYSS
KYYRYGIKSGEIKACDIEKEEKDIKYTLKYKNLAKEIKLSVPGIHNVYNSLAAICVGLEFGINVEDMAEALKDFQPGKMR
LNIIKIGDIKIIDDVYNANPDSMKAALSVLRDLPAKRKIAVLGDMLELGEYAVEAHKEIGKLVADSHIDILITSGDMSKY
IAEESKVCGMKENNIFVCNTNKEVNEILKDIIKEGDSILVKGSRGMKMEQIVQFLQERVK
>Mature_460_residues
MELLIDEIVRATEGKLIKGNLKDKVMGISTDSRKVKKDELFIPLKGEKYDGEDFVKDAIDKGASAVITARLDNVECLKDF
SVNVIYVNETLEALHKIASYYRKKFNIPFIAVTGSSGKTTTKDMIAQILSKKYKVLKTEGNFNNEIGLPLTIFNLEDTHQ
IAVVEMGMNGFGEIRRLKNIAEPQVAVFTNIGVAHIEKLGSRENILKAKTELIENFKEENVIVLNADDDMLSKIPLQYSS
KYYRYGIKSGEIKACDIEKEEKDIKYTLKYKNLAKEIKLSVPGIHNVYNSLAAICVGLEFGINVEDMAEALKDFQPGKMR
LNIIKIGDIKIIDDVYNANPDSMKAALSVLRDLPAKRKIAVLGDMLELGEYAVEAHKEIGKLVADSHIDILITSGDMSKY
IAEESKVCGMKENNIFVCNTNKEVNEILKDIIKEGDSILVKGSRGMKMEQIVQFLQERVK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=436, Percent_Identity=33.4862385321101, Blast_Score=216, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51543; Mature: 51543

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLIDEIVRATEGKLIKGNLKDKVMGISTDSRKVKKDELFIPLKGEKYDGEDFVKDAID
CCCHHHHHHHHCCCCEEECCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
KGASAVITARLDNVECLKDFSVNVIYVNETLEALHKIASYYRKKFNIPFIAVTGSSGKTT
CCCCEEEEEECCCHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
TKDMIAQILSKKYKVLKTEGNFNNEIGLPLTIFNLEDTHQIAVVEMGMNGFGEIRRLKNI
HHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHC
AEPQVAVFTNIGVAHIEKLGSRENILKAKTELIENFKEENVIVLNADDDMLSKIPLQYSS
CCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHCC
KYYRYGIKSGEIKACDIEKEEKDIKYTLKYKNLAKEIKLSVPGIHNVYNSLAAICVGLEF
CHHEECCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHEECCCCHHHHHHHHHHHHHHHHC
GINVEDMAEALKDFQPGKMRLNIIKIGDIKIIDDVYNANPDSMKAALSVLRDLPAKRKIA
CCCHHHHHHHHHCCCCCCEEEEEEEECCEEEEHHHHCCCHHHHHHHHHHHHHCCCCCCHH
VLGDMLELGEYAVEAHKEIGKLVADSHIDILITSGDMSKYIAEESKVCGMKENNIFVCNT
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCEEEECC
NKEVNEILKDIIKEGDSILVKGSRGMKMEQIVQFLQERVK
CHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MELLIDEIVRATEGKLIKGNLKDKVMGISTDSRKVKKDELFIPLKGEKYDGEDFVKDAID
CCCHHHHHHHHCCCCEEECCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
KGASAVITARLDNVECLKDFSVNVIYVNETLEALHKIASYYRKKFNIPFIAVTGSSGKTT
CCCCEEEEEECCCHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
TKDMIAQILSKKYKVLKTEGNFNNEIGLPLTIFNLEDTHQIAVVEMGMNGFGEIRRLKNI
HHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHC
AEPQVAVFTNIGVAHIEKLGSRENILKAKTELIENFKEENVIVLNADDDMLSKIPLQYSS
CCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHCC
KYYRYGIKSGEIKACDIEKEEKDIKYTLKYKNLAKEIKLSVPGIHNVYNSLAAICVGLEF
CHHEECCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHEECCCCHHHHHHHHHHHHHHHHC
GINVEDMAEALKDFQPGKMRLNIIKIGDIKIIDDVYNANPDSMKAALSVLRDLPAKRKIA
CCCHHHHHHHHHCCCCCCEEEEEEEECCEEEEHHHHCCCHHHHHHHHHHHHHCCCCCCHH
VLGDMLELGEYAVEAHKEIGKLVADSHIDILITSGDMSKYIAEESKVCGMKENNIFVCNT
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCEEEECC
NKEVNEILKDIIKEGDSILVKGSRGMKMEQIVQFLQERVK
CHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]