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The map label for this gene is nudF [H]

Identifier: 167040190

GI number: 167040190

Start: 1575567

End: 1576106

Strand: Direct

Name: nudF [H]

Synonym: Teth514_1552

Alternate gene names: 167040190

Gene position: 1575567-1576106 (Clockwise)

Preceding gene: 167040189

Following gene: 167040191

Centisome position: 64.12

GC content: 30.37

Gene sequence:

>540_bases
TTGGAACAAAAAGAAGTTACAGTTAACACCAACAAGATTTTTGAAGGGAAAATAATAAATTTAAGAGTAGATGAGGTAAA
ACTGCCTAATGGAAAAGTTACTACGCGAGAAATAGTTGAGCATCCTGGCGGTGTATCCATAGTAGCAGTTAATGAAGAAG
GAAAGATTTTATTAGTCAAACAGTATAGAAAACCTGCAGAGGAGTCTTTATTAGAAATACCTGCAGGAAAATTAGAAAAG
GGTGAAGACCCTTTGATTTGTGCTAAAAGAGAGTTATTAGAAGAGACAGGTTATGAAGCAGGTTTTATAAAGCAGTTGAT
TACTTTTTATACTACTCCCGGTTTTTCTAATGAAAAAATGTATTTGTATTTTGCAAAAGATTTAAAAAAATACACTGCTC
AACCTGATGAGGATGAATTTTTAGAAGTATATGAATATACTCCAGAGGAATTGTGGGAAATGATTTTACAAAATCAAATA
AAAGATTCTAAAACGATAATTGGAATTTTATATTATTTAAAAATGAGGAATAGTTTATGA

Upstream 100 bases:

>100_bases
CAGCATAATAAAGAGTGTTTCCCACTATAATCATTTTTCTCACCTCATTTCTATAGTATATATTTATTGCTAAAATTATA
CATAGAAAGGAAGATGAATT

Downstream 100 bases:

>100_bases
AATTTTACGGAGATTTGCATGTTCATGTTGCAAGGAGTATAAAAGGAGCTCCTGTAAAAATTGCGGCCTCAAAAAATTTA
ACTGTTTTAAATATATTAGA

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MEQKEVTVNTNKIFEGKIINLRVDEVKLPNGKVTTREIVEHPGGVSIVAVNEEGKILLVKQYRKPAEESLLEIPAGKLEK
GEDPLICAKRELLEETGYEAGFIKQLITFYTTPGFSNEKMYLYFAKDLKKYTAQPDEDEFLEVYEYTPEELWEMILQNQI
KDSKTIIGILYYLKMRNSL

Sequences:

>Translated_179_residues
MEQKEVTVNTNKIFEGKIINLRVDEVKLPNGKVTTREIVEHPGGVSIVAVNEEGKILLVKQYRKPAEESLLEIPAGKLEK
GEDPLICAKRELLEETGYEAGFIKQLITFYTTPGFSNEKMYLYFAKDLKKYTAQPDEDEFLEVYEYTPEELWEMILQNQI
KDSKTIIGILYYLKMRNSL
>Mature_179_residues
MEQKEVTVNTNKIFEGKIINLRVDEVKLPNGKVTTREIVEHPGGVSIVAVNEEGKILLVKQYRKPAEESLLEIPAGKLEK
GEDPLICAKRELLEETGYEAGFIKQLITFYTTPGFSNEKMYLYFAKDLKKYTAQPDEDEFLEVYEYTPEELWEMILQNQI
KDSKTIIGILYYLKMRNSL

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788810, Length=149, Percent_Identity=34.2281879194631, Blast_Score=72, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319587, Length=136, Percent_Identity=34.5588235294118, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20688; Mature: 20688

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKEVTVNTNKIFEGKIINLRVDEVKLPNGKVTTREIVEHPGGVSIVAVNEEGKILLVK
CCCCEEEEECCEEECCEEEEEEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCEEEEH
QYRKPAEESLLEIPAGKLEKGEDPLICAKRELLEETGYEAGFIKQLITFYTTPGFSNEKM
HHCCCHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEE
YLYFAKDLKKYTAQPDEDEFLEVYEYTPEELWEMILQNQIKDSKTIIGILYYLKMRNSL
EEEEEHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEQKEVTVNTNKIFEGKIINLRVDEVKLPNGKVTTREIVEHPGGVSIVAVNEEGKILLVK
CCCCEEEEECCEEECCEEEEEEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCEEEEH
QYRKPAEESLLEIPAGKLEKGEDPLICAKRELLEETGYEAGFIKQLITFYTTPGFSNEKM
HHCCCHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEE
YLYFAKDLKKYTAQPDEDEFLEVYEYTPEELWEMILQNQIKDSKTIIGILYYLKMRNSL
EEEEEHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]