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The map label for this gene is cmtB [H]

Identifier: 162420932

GI number: 162420932

Start: 357634

End: 358077

Strand: Direct

Name: cmtB [H]

Synonym: YpAngola_A0342

Alternate gene names: 162420932

Gene position: 357634-358077 (Clockwise)

Preceding gene: 162419840

Following gene: 162420119

Centisome position: 7.94

GC content: 47.75

Gene sequence:

>444_bases
ATGTTAAAAACACTGTTAACGTCGGATGTCATTCAGGTTGTCAGCCAGGCAAAAGATTGGCGTGATGCTATCGCGATCTC
ATGCCAACCGCTTATCGATAATGGTGCGGTAGAAGCTCGCTATGTTGAAGCGATTTATCGCTCCCATGAAGCTATTGGCC
CTTATTACGTAGTCGGTCCTGGTATTGCGATGCCACATGCCCGCCCGGAAGACGGCGTAAATAGACTCTCTCTGGCACTA
ACTGTCATCACCGAGGGGGTCACTTTCAATGCAGAAGGTAACGACCCGGTAAAACTGCTCATCGTGTTAGCGGCAACAGA
CAGCAATAGCCATATTGAGGCTATTTCCCAACTCGCTCAATTGTTCGATACCGCTAGTGATGTGCAAGCCTTACTTAATG
CTAAAACACCGCAGGATATTCTGTCAGTGATTGCACGCTATTAA

Upstream 100 bases:

>100_bases
AGCGCTACCAGATAGCGCTATCAATTGAGATCCAGATCACATTAATAAGCATGATAAGTATCTACTCTCTGGTTAACAGT
CATAGGGAGTCATAAATTTC

Downstream 100 bases:

>100_bases
TCACGGTATTCAGTTCGCATATATAAAATCACTCTCCAATAGATTTCAAGATACAGAGCGGCGGCAACTGGATGGCACCC
AAGAGCGGACACAAGTCAGT

Product: phosphotransferase enzyme II, A component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MLKTLLTSDVIQVVSQAKDWRDAIAISCQPLIDNGAVEARYVEAIYRSHEAIGPYYVVGPGIAMPHARPEDGVNRLSLAL
TVITEGVTFNAEGNDPVKLLIVLAATDSNSHIEAISQLAQLFDTASDVQALLNAKTPQDILSVIARY

Sequences:

>Translated_147_residues
MLKTLLTSDVIQVVSQAKDWRDAIAISCQPLIDNGAVEARYVEAIYRSHEAIGPYYVVGPGIAMPHARPEDGVNRLSLAL
TVITEGVTFNAEGNDPVKLLIVLAATDSNSHIEAISQLAQLFDTASDVQALLNAKTPQDILSVIARY
>Mature_147_residues
MLKTLLTSDVIQVVSQAKDWRDAIAISCQPLIDNGAVEARYVEAIYRSHEAIGPYYVVGPGIAMPHARPEDGVNRLSLAL
TVITEGVTFNAEGNDPVKLLIVLAATDSNSHIEAISQLAQLFDTASDVQALLNAKTPQDILSVIARY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=144, Percent_Identity=37.5, Blast_Score=115, Evalue=1e-27,
Organism=Escherichia coli, GI1790755, Length=135, Percent_Identity=38.5185185185185, Blast_Score=111, Evalue=2e-26,
Organism=Escherichia coli, GI1789302, Length=137, Percent_Identity=42.3357664233577, Blast_Score=101, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15843; Mature: 15843

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTLLTSDVIQVVSQAKDWRDAIAISCQPLIDNGAVEARYVEAIYRSHEAIGPYYVVGP
CCHHHHHHHHHHHHHHHHHHHHHHHEECEECCCCCCHHHHHHHHHHHHHHCCCCEEEECC
GIAMPHARPEDGVNRLSLALTVITEGVTFNAEGNDPVKLLIVLAATDSNSHIEAISQLAQ
CEECCCCCCCCCHHHHHHHHHEEECCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHH
LFDTASDVQALLNAKTPQDILSVIARY
HHCCHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLKTLLTSDVIQVVSQAKDWRDAIAISCQPLIDNGAVEARYVEAIYRSHEAIGPYYVVGP
CCHHHHHHHHHHHHHHHHHHHHHHHEECEECCCCCCHHHHHHHHHHHHHHCCCCEEEECC
GIAMPHARPEDGVNRLSLALTVITEGVTFNAEGNDPVKLLIVLAATDSNSHIEAISQLAQ
CEECCCCCCCCCHHHHHHHHHEEECCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHH
LFDTASDVQALLNAKTPQDILSVIARY
HHCCHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]