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The map label for this gene is pit [H]

Identifier: 162149585

GI number: 162149585

Start: 3907528

End: 3908883

Strand: Direct

Name: pit [H]

Synonym: GDI_3824

Alternate gene names: 162149585

Gene position: 3907528-3908883 (Clockwise)

Preceding gene: 162149584

Following gene: 162149588

Centisome position: 99.07

GC content: 67.33

Gene sequence:

>1356_bases
ATGCTGCATTTCGCACCTTATTCGCTGTTTTCGATCCTCGCGCTGCTCGCCTGCTTCCTGCTGGTCTGCGTGTTCGAATT
CGTCAACGGATTCCATGACACCGCCAATGCGGTGGCGACGGTCATCTATACCAATTCGCTGAAGCCCCGGACGGCGGTGG
TCTGGTCGGGGCTGATGAACTTCGTCGGCGTCGTACTGAGCGGCATCAGCGTCGCCTACGGCCTGGTGGAACTGCTGCCG
GCCGACGTGCTGTCGCCGCCCAATGGCGACCCGGCGGTGCCGATGCTGATCTCGCTGTTCGGCACCGCGCTGGCGTGGAA
CCTGTTCACCTGGTGGTTCGGTATTCCCAATTCCAGTTCCCATTGCGTGATCGGCGCCCTGATCGGGATCGCGGTCGGCG
ACGCGCTGCTGCATACGCGCGACCTGGGCAATTCGGTGGACTGGTCGCAGATCTGGAAGATCCTGCGGGCGCTGGCGATT
TCGCCGCTGCTGGGGCTGGTGGGGGCGGGGGCGCTGTATTTCGCGCTGAAGCATCTGGTGCGCGACCCGGCGCTGTACCA
GCCGCCGCAGGGTGACGCGCCGCCGCGCGGCTGGGTCAAGGGGCTGCTGATCCTGACCTGCACCGGCGTCAGCTTCTCGC
ACGGCAGCAATGACGGGCAGAAGAGCATCGGGCTGATCATGCTGACGATTATCGGCCTGCTGCCCGCGACCTTCGCGCTG
AATCCCGCAACCGCACCCGATATCGCGCACATTGCCGAACGCGCCGCGATCGCGGCACCGCTGCTGGATGCCTACGACCA
TGACGGGCTGCGGGCCGAGGCGCTGGCATCCGCCGCGCGCCTGTCGCACACCCAGGCCGACCATGTGGTGGCGTCGCAAC
TGCGTGCCGACCTGTACGAGGTCGTGGCCGGGCTGAAGGCGGTTTCGGCCAGCAGGGCCGCCAGCGGTGCCGACCGCGCG
GTCGCGGCCCGCATCGGGCGGGAACTGCGTCCCACGGTGGAATATGCGCCCTGGTGGGTGCGGCTGCTCAGCGCGCTGTG
CCTGGGGATCGGCACCATGGTCGGCTATCGCCGGATCGTTCACACGCTGGGCGAGAAGATCGGCAATACCCACCTGACGC
CCGCGCAGGGCGCGTCGGCGGAACTGGTCGGCGCCGGGCTGATCGCCACCGCGGGCTATACCGGCCTGCCGGTCAGCACC
ACCCACATCATCACCGCCGGCATCGCCGGCACCATGCTGGGATCGGGATCGGGCATCAATCGCGGCATGCTGATGAAGAT
CGCCCTGGCCTGGATCTGCACCCTGCCGGTCACGATCGCGGTCGCGGCCAGCCTGTTCTACGTCCTGGGAGGGTAA

Upstream 100 bases:

>100_bases
GGGTACCCTGTGAAATGTTTTGTGAATGTCCTGATATTATATGAAAATATGATATTATTTCTGTAGTTGTTCGAAATCAC
TGCAACCGGTCTTCGGCATC

Downstream 100 bases:

>100_bases
GTCTGTCCGCAAACGCGCGCTGGTGGCGATCCGGCGTGCGGTTCCGGCTGGCTGGGATTACAGGGTGGAGCGGTGCCGGC
TTGCCAGCTTCTCCCGTGCT

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MLHFAPYSLFSILALLACFLLVCVFEFVNGFHDTANAVATVIYTNSLKPRTAVVWSGLMNFVGVVLSGISVAYGLVELLP
ADVLSPPNGDPAVPMLISLFGTALAWNLFTWWFGIPNSSSHCVIGALIGIAVGDALLHTRDLGNSVDWSQIWKILRALAI
SPLLGLVGAGALYFALKHLVRDPALYQPPQGDAPPRGWVKGLLILTCTGVSFSHGSNDGQKSIGLIMLTIIGLLPATFAL
NPATAPDIAHIAERAAIAAPLLDAYDHDGLRAEALASAARLSHTQADHVVASQLRADLYEVVAGLKAVSASRAASGADRA
VAARIGRELRPTVEYAPWWVRLLSALCLGIGTMVGYRRIVHTLGEKIGNTHLTPAQGASAELVGAGLIATAGYTGLPVST
THIITAGIAGTMLGSGSGINRGMLMKIALAWICTLPVTIAVAASLFYVLGG

Sequences:

>Translated_451_residues
MLHFAPYSLFSILALLACFLLVCVFEFVNGFHDTANAVATVIYTNSLKPRTAVVWSGLMNFVGVVLSGISVAYGLVELLP
ADVLSPPNGDPAVPMLISLFGTALAWNLFTWWFGIPNSSSHCVIGALIGIAVGDALLHTRDLGNSVDWSQIWKILRALAI
SPLLGLVGAGALYFALKHLVRDPALYQPPQGDAPPRGWVKGLLILTCTGVSFSHGSNDGQKSIGLIMLTIIGLLPATFAL
NPATAPDIAHIAERAAIAAPLLDAYDHDGLRAEALASAARLSHTQADHVVASQLRADLYEVVAGLKAVSASRAASGADRA
VAARIGRELRPTVEYAPWWVRLLSALCLGIGTMVGYRRIVHTLGEKIGNTHLTPAQGASAELVGAGLIATAGYTGLPVST
THIITAGIAGTMLGSGSGINRGMLMKIALAWICTLPVTIAVAASLFYVLGG
>Mature_451_residues
MLHFAPYSLFSILALLACFLLVCVFEFVNGFHDTANAVATVIYTNSLKPRTAVVWSGLMNFVGVVLSGISVAYGLVELLP
ADVLSPPNGDPAVPMLISLFGTALAWNLFTWWFGIPNSSSHCVIGALIGIAVGDALLHTRDLGNSVDWSQIWKILRALAI
SPLLGLVGAGALYFALKHLVRDPALYQPPQGDAPPRGWVKGLLILTCTGVSFSHGSNDGQKSIGLIMLTIIGLLPATFAL
NPATAPDIAHIAERAAIAAPLLDAYDHDGLRAEALASAARLSHTQADHVVASQLRADLYEVVAGLKAVSASRAASGADRA
VAARIGRELRPTVEYAPWWVRLLSALCLGIGTMVGYRRIVHTLGEKIGNTHLTPAQGASAELVGAGLIATAGYTGLPVST
THIITAGIAGTMLGSGSGINRGMLMKIALAWICTLPVTIAVAASLFYVLGG

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=476, Percent_Identity=36.9747899159664, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1789360, Length=469, Percent_Identity=36.0341151385927, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 47162; Mature: 47162

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHFAPYSLFSILALLACFLLVCVFEFVNGFHDTANAVATVIYTNSLKPRTAVVWSGLMN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
FVGVVLSGISVAYGLVELLPADVLSPPNGDPAVPMLISLFGTALAWNLFTWWFGIPNSSS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HCVIGALIGIAVGDALLHTRDLGNSVDWSQIWKILRALAISPLLGLVGAGALYFALKHLV
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDPALYQPPQGDAPPRGWVKGLLILTCTGVSFSHGSNDGQKSIGLIMLTIIGLLPATFAL
HCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
NPATAPDIAHIAERAAIAAPLLDAYDHDGLRAEALASAARLSHTQADHVVASQLRADLYE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAGLKAVSASRAASGADRAVAARIGRELRPTVEYAPWWVRLLSALCLGIGTMVGYRRIV
HHHHHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
HTLGEKIGNTHLTPAQGASAELVGAGLIATAGYTGLPVSTTHIITAGIAGTMLGSGSGIN
HHHHHHHCCCCCCCCCCCCCHHHHCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
RGMLMKIALAWICTLPVTIAVAASLFYVLGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLHFAPYSLFSILALLACFLLVCVFEFVNGFHDTANAVATVIYTNSLKPRTAVVWSGLMN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
FVGVVLSGISVAYGLVELLPADVLSPPNGDPAVPMLISLFGTALAWNLFTWWFGIPNSSS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HCVIGALIGIAVGDALLHTRDLGNSVDWSQIWKILRALAISPLLGLVGAGALYFALKHLV
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDPALYQPPQGDAPPRGWVKGLLILTCTGVSFSHGSNDGQKSIGLIMLTIIGLLPATFAL
HCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
NPATAPDIAHIAERAAIAAPLLDAYDHDGLRAEALASAARLSHTQADHVVASQLRADLYE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAGLKAVSASRAASGADRAVAARIGRELRPTVEYAPWWVRLLSALCLGIGTMVGYRRIV
HHHHHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
HTLGEKIGNTHLTPAQGASAELVGAGLIATAGYTGLPVSTTHIITAGIAGTMLGSGSGIN
HHHHHHHCCCCCCCCCCCCCHHHHCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
RGMLMKIALAWICTLPVTIAVAASLFYVLGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]