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The map label for this gene is nitA [H]

Identifier: 162149505

GI number: 162149505

Start: 3825461

End: 3826420

Strand: Direct

Name: nitA [H]

Synonym: GDI_3743

Alternate gene names: 162149505

Gene position: 3825461-3826420 (Clockwise)

Preceding gene: 162149504

Following gene: 162149506

Centisome position: 96.99

GC content: 68.02

Gene sequence:

>960_bases
ATGTCACGTATCGTTCGCGCCGCCGCCGTCCAGATCAGCCCCGTGCTGGGGGATGACGGCATGGGAACCGCCCGGAAAGT
CTGCCGGACCATCCGCGAGGCCGCCGAAAAGGGCGTGAAGCTGGCGGTGTTTCCCGAAACCTTCGTGCCGTACTACCCCT
ATTTCTCGTTCATCAAGCCGGCGTTCCGCTTCGGTGCGGAGCATCTGGCGCTGTATGAACGGGCGGTGAGCGTTCCCGGC
CCCATCACCGACATGGTGGCCGAGGCCGCGCGCGAGACCGGCATGGTCGTCGTGCTGGGGGTGAACGAGCGCGACCACGG
CACGCTGTACAACGCGCAGGTCATCTTCGACGCCACGGGCGAGATCGTGCTCAAGCGCCGGAAAATCACCCCGACCTATC
ACGAGCGGATGGTGTGGGGGCAGGGCGACGGGTCGGGGCTGACGGTGGTGGACAGCGCCGCCGGGCGCATCGGGGCGCTG
GCGTGCTGGGAGCATTACAATCCGCTGGCCCGCTATGCCCTGATGGCGCAGCACGAGGAGATCCATTGCGCCCAGTTCCC
GGGCTCGCTGGTCGGGCAGGTCTTCGCGGACCAGATGGAGGTCACGATCCGCCATCACGCGCTGGAATCGGGCTGTTTCG
TGGTCAACGCGACGGGCTGGCTGGACGACGAACAGGTCCGGCAGGTCGCGGGCGACCCGGCGCTGGAGGGGCCGCTGCGG
GGCGGGTGCTTCACCGCCATCGTCTCGCCCGAGGGGCGGCTGCTGGGCACGCCGGTCACGGACGGCGAGGGCATGGTCAT
TGCCGACCTGGATTTCGCGCTGATCACCAAGCGCAAGCGGATGATGGATTCGGTCGGCCATTACGCGCGCCCCGAACTGC
TGAGCCTGCTGCTGGACCGGCGTCCGGCCCGCACCGTCCATTATGTGGACGACGCGGCGTGCGAAGGAGGTGCGCCATGA

Upstream 100 bases:

>100_bases
TGTTTGAACACGCCGGGCGCGGCCTTCGGACCGCTCCCGCTCGCCAGCCCACATTTTCACACAGGCTCTGACTGGAACTG
GAACGGGAAGGACCGCCAGC

Downstream 100 bases:

>100_bases
CCGTTCCGGCATCCGGCACGCTGGCGGGGCGCCAGCTTGTCACCGACCTGCAATCCTTCGGCCTGCAGGTCGACGGGCAG
GTCGGCGGGATTGCCCGCAA

Product: nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSRIVRAAAVQISPVLGDDGMGTARKVCRTIREAAEKGVKLAVFPETFVPYYPYFSFIKPAFRFGAEHLALYERAVSVPG
PITDMVAEAARETGMVVVLGVNERDHGTLYNAQVIFDATGEIVLKRRKITPTYHERMVWGQGDGSGLTVVDSAAGRIGAL
ACWEHYNPLARYALMAQHEEIHCAQFPGSLVGQVFADQMEVTIRHHALESGCFVVNATGWLDDEQVRQVAGDPALEGPLR
GGCFTAIVSPEGRLLGTPVTDGEGMVIADLDFALITKRKRMMDSVGHYARPELLSLLLDRRPARTVHYVDDAACEGGAP

Sequences:

>Translated_319_residues
MSRIVRAAAVQISPVLGDDGMGTARKVCRTIREAAEKGVKLAVFPETFVPYYPYFSFIKPAFRFGAEHLALYERAVSVPG
PITDMVAEAARETGMVVVLGVNERDHGTLYNAQVIFDATGEIVLKRRKITPTYHERMVWGQGDGSGLTVVDSAAGRIGAL
ACWEHYNPLARYALMAQHEEIHCAQFPGSLVGQVFADQMEVTIRHHALESGCFVVNATGWLDDEQVRQVAGDPALEGPLR
GGCFTAIVSPEGRLLGTPVTDGEGMVIADLDFALITKRKRMMDSVGHYARPELLSLLLDRRPARTVHYVDDAACEGGAP
>Mature_318_residues
SRIVRAAAVQISPVLGDDGMGTARKVCRTIREAAEKGVKLAVFPETFVPYYPYFSFIKPAFRFGAEHLALYERAVSVPGP
ITDMVAEAARETGMVVVLGVNERDHGTLYNAQVIFDATGEIVLKRRKITPTYHERMVWGQGDGSGLTVVDSAAGRIGALA
CWEHYNPLARYALMAQHEEIHCAQFPGSLVGQVFADQMEVTIRHHALESGCFVVNATGWLDDEQVRQVAGDPALEGPLRG
GCFTAIVSPEGRLLGTPVTDGEGMVIADLDFALITKRKRMMDSVGHYARPELLSLLLDRRPARTVHYVDDAACEGGAP

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=286, Percent_Identity=25.1748251748252, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17557111, Length=304, Percent_Identity=31.9078947368421, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322027, Length=186, Percent_Identity=37.6344086021505, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 34755; Mature: 34624

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50007 PIPLC_X_DOMAIN ; PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIVRAAAVQISPVLGDDGMGTARKVCRTIREAAEKGVKLAVFPETFVPYYPYFSFIKP
CCCHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHCCCCHHHHHHHH
AFRFGAEHLALYERAVSVPGPITDMVAEAARETGMVVVLGVNERDHGTLYNAQVIFDATG
HHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEECCC
EIVLKRRKITPTYHERMVWGQGDGSGLTVVDSAAGRIGALACWEHYNPLARYALMAQHEE
EEEEEECCCCCHHHHEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCC
IHCAQFPGSLVGQVFADQMEVTIRHHALESGCFVVNATGWLDDEQVRQVAGDPALEGPLR
CCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCC
GGCFTAIVSPEGRLLGTPVTDGEGMVIADLDFALITKRKRMMDSVGHYARPELLSLLLDR
CCEEEEEECCCCCEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
RPARTVHYVDDAACEGGAP
CCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
SRIVRAAAVQISPVLGDDGMGTARKVCRTIREAAEKGVKLAVFPETFVPYYPYFSFIKP
CCHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHCCCCHHHHHHHH
AFRFGAEHLALYERAVSVPGPITDMVAEAARETGMVVVLGVNERDHGTLYNAQVIFDATG
HHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEECCC
EIVLKRRKITPTYHERMVWGQGDGSGLTVVDSAAGRIGALACWEHYNPLARYALMAQHEE
EEEEEECCCCCHHHHEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCC
IHCAQFPGSLVGQVFADQMEVTIRHHALESGCFVVNATGWLDDEQVRQVAGDPALEGPLR
CCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCC
GGCFTAIVSPEGRLLGTPVTDGEGMVIADLDFALITKRKRMMDSVGHYARPELLSLLLDR
CCEEEEEECCCCCEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
RPARTVHYVDDAACEGGAP
CCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]