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The map label for this gene is lacI [H]

Identifier: 162149497

GI number: 162149497

Start: 3818836

End: 3819894

Strand: Direct

Name: lacI [H]

Synonym: GDI_3735

Alternate gene names: 162149497

Gene position: 3818836-3819894 (Clockwise)

Preceding gene: 162149495

Following gene: 162149498

Centisome position: 96.82

GC content: 69.41

Gene sequence:

>1059_bases
CTGAAGAACGGAAAACGCTATGCGTCCTCGACCGATGTGGCACGCCTGGCCGGCGTGTCGCAATCCGCGGTGTCCCGTGC
CTACAAGAAGGGCGGTTACGTCTCGCCCGAGATGCGCAGCCGGATAATGGCGGCGGCGGCCGAACTGGAATATCGGCCCA
GCCTGATCCCCCGGATCATGCTGACCGAACGGTCGAACCTGATCGCGGTCGTGGTGGGGGGACTGTACAATCCGCTCTAT
GCCGGGATCATCGACACCTTGTCGGTCAGGCTGGAAGATGCGGGATTACAGACGCTGCTGGTGCATGCCGAAAGCGGCCA
TATGCTGGACATCGCGCTGCCGCGCCTTGCCAGCTACCGGGTCGATGCGATCGTCAGTCCGCTGGCGGTCATGTCCGCCG
CGACGGCCGAGGCGCTGGACCGGCTGGGCCTTCCCGTGGTGTCGTTCAATACCCCGGTGCGGGGCGGCCTGATCTCGACG
ATTTCGACCGACGGGCTGGCGGCGGGGCGGGACATCGCGGCCCTGTTCGCGCGGCGTGGAGCCCGGCGCCCCGGCTTCGT
CCAGGGGCCGAAGGGCAGTCCGGCGAGCGACGACCGGGAACGGGGATATCGCCGGGCACTGCGCGGGCTGGGGCTGGGCG
CGCCGCGCACGGCGCCGGGCGATTTCCGGTATGAGGGCGGGGTCGCGGCGGCGCATGCCCTGCTGGGCGGGCGGGATGCA
TGCGATGCGATCTTCTGCGCGAACGACCTGCTGGCGCTCGGGGTGATGGACACCGCGCGGCGGCAGTTCGGGCTGCGCGT
GCCCGAGGACCTGATGGTGGCCGGCTTCGACGACATCGATATGGCGTCATGGAGCAGCTACGGCCTGACCAGCTTCGTCC
AGGATGTCGACCTCATGGCCCGGCACGCCGTGCACCTGCTGACACAGGCGCTGGAGACGCCCGGACGCACGGGCGAGCAC
GTCGTGATTCCCGCAACCCTGGTGGAGCGGGCCTCGACGGGGGGTGGTGATGCAGGGGGCGATGGCGGGCGCGACCGGGG
TGTGCGCTGCGACGATTGA

Upstream 100 bases:

>100_bases
TCACACGAGGATGGAAGTCAAGGTCTTTCGCGCGGTACTTCCTTTATTTTACTGGACTCTATAGAATACGGACCCGCGCG
CGCATCGTCGGGAGGCTGAA

Downstream 100 bases:

>100_bases
CTGGCCCGACGCGCCGGAGCTTTCTACACTGGACGGCATGACGGACGATCGATTTTCCCGCCTGAGCCGCCGTGGCGCGC
TTTCCTCGCTCATGGCCCTG

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKNGKRYASSTDVARLAGVSQSAVSRAYKKGGYVSPEMRSRIMAAAAELEYRPSLIPRIMLTERSNLIAVVVGGLYNPLY
AGIIDTLSVRLEDAGLQTLLVHAESGHMLDIALPRLASYRVDAIVSPLAVMSAATAEALDRLGLPVVSFNTPVRGGLIST
ISTDGLAAGRDIAALFARRGARRPGFVQGPKGSPASDDRERGYRRALRGLGLGAPRTAPGDFRYEGGVAAAHALLGGRDA
CDAIFCANDLLALGVMDTARRQFGLRVPEDLMVAGFDDIDMASWSSYGLTSFVQDVDLMARHAVHLLTQALETPGRTGEH
VVIPATLVERASTGGGDAGGDGGRDRGVRCDD

Sequences:

>Translated_352_residues
MKNGKRYASSTDVARLAGVSQSAVSRAYKKGGYVSPEMRSRIMAAAAELEYRPSLIPRIMLTERSNLIAVVVGGLYNPLY
AGIIDTLSVRLEDAGLQTLLVHAESGHMLDIALPRLASYRVDAIVSPLAVMSAATAEALDRLGLPVVSFNTPVRGGLIST
ISTDGLAAGRDIAALFARRGARRPGFVQGPKGSPASDDRERGYRRALRGLGLGAPRTAPGDFRYEGGVAAAHALLGGRDA
CDAIFCANDLLALGVMDTARRQFGLRVPEDLMVAGFDDIDMASWSSYGLTSFVQDVDLMARHAVHLLTQALETPGRTGEH
VVIPATLVERASTGGGDAGGDGGRDRGVRCDD
>Mature_352_residues
MKNGKRYASSTDVARLAGVSQSAVSRAYKKGGYVSPEMRSRIMAAAAELEYRPSLIPRIMLTERSNLIAVVVGGLYNPLY
AGIIDTLSVRLEDAGLQTLLVHAESGHMLDIALPRLASYRVDAIVSPLAVMSAATAEALDRLGLPVVSFNTPVRGGLIST
ISTDGLAAGRDIAALFARRGARRPGFVQGPKGSPASDDRERGYRRALRGLGLGAPRTAPGDFRYEGGVAAAHALLGGRDA
CDAIFCANDLLALGVMDTARRQFGLRVPEDLMVAGFDDIDMASWSSYGLTSFVQDVDLMARHAVHLLTQALETPGRTGEH
VVIPATLVERASTGGGDAGGDGGRDRGVRCDD

Specific function: Binds to the 14-bp operator sequence 5'-TGGGAGCGCTCCCA- 3' in the upstream regions of cellulase genes; these operator sequences are modulated by cellobiose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=345, Percent_Identity=30.4347826086957, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=28.8753799392097, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789068, Length=286, Percent_Identity=29.7202797202797, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=25.4437869822485, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=29.607250755287, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI48994940, Length=280, Percent_Identity=26.7857142857143, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787580, Length=282, Percent_Identity=25.886524822695, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786540, Length=331, Percent_Identity=28.7009063444109, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790715, Length=283, Percent_Identity=24.7349823321555, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789202, Length=298, Percent_Identity=25.503355704698, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789456, Length=341, Percent_Identity=23.1671554252199, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37149; Mature: 37149

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNGKRYASSTDVARLAGVSQSAVSRAYKKGGYVSPEMRSRIMAAAAELEYRPSLIPRIM
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
LTERSNLIAVVVGGLYNPLYAGIIDTLSVRLEDAGLQTLLVHAESGHMLDIALPRLASYR
EECCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEHHHHHHHH
VDAIVSPLAVMSAATAEALDRLGLPVVSFNTPVRGGLISTISTDGLAAGRDIAALFARRG
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEHHCCCCCCCCCHHHHHHHHHCC
ARRPGFVQGPKGSPASDDRERGYRRALRGLGLGAPRTAPGDFRYEGGVAAAHALLGGRDA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHCCCHH
CDAIFCANDLLALGVMDTARRQFGLRVPEDLMVAGFDDIDMASWSSYGLTSFVQDVDLMA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHH
RHAVHLLTQALETPGRTGEHVVIPATLVERASTGGGDAGGDGGRDRGVRCDD
HHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNGKRYASSTDVARLAGVSQSAVSRAYKKGGYVSPEMRSRIMAAAAELEYRPSLIPRIM
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
LTERSNLIAVVVGGLYNPLYAGIIDTLSVRLEDAGLQTLLVHAESGHMLDIALPRLASYR
EECCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEHHHHHHHH
VDAIVSPLAVMSAATAEALDRLGLPVVSFNTPVRGGLISTISTDGLAAGRDIAALFARRG
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEHHCCCCCCCCCHHHHHHHHHCC
ARRPGFVQGPKGSPASDDRERGYRRALRGLGLGAPRTAPGDFRYEGGVAAAHALLGGRDA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHCCCHH
CDAIFCANDLLALGVMDTARRQFGLRVPEDLMVAGFDDIDMASWSSYGLTSFVQDVDLMA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHH
RHAVHLLTQALETPGRTGEHVVIPATLVERASTGGGDAGGDGGRDRGVRCDD
HHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10224066 [H]