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The map label for this gene is afr [H]

Identifier: 162149494

GI number: 162149494

Start: 3816235

End: 3817230

Strand: Direct

Name: afr [H]

Synonym: GDI_3732

Alternate gene names: 162149494

Gene position: 3816235-3817230 (Clockwise)

Preceding gene: 162149493

Following gene: 162149495

Centisome position: 96.76

GC content: 68.78

Gene sequence:

>996_bases
ATGACTGCAATGCGCTGGGGTTTGATCGGTGCGACCATCATCGGCCGGGAATGGATGATCCGCGCCATTCGTGATGCCGG
CGGCACCATCACGTCCGTCTACAGCACCGACGCCGAGCGCGGCCGGCGCTATGCCCGCGAATTCGACATTCCGGATTCGA
CGACCGACCTCGACACGCTTCTGCAAAACGTCGATGCGGTCTATATTTCCACGACCAACGACCGTCATCATGCGGAATGC
CTGGCGGCGGCGCGGGCGGGCAGGCATGTCCTGTGCGAAAAGCCGCTGGCGACACGCTACCAGGACGCGGTCGAGATGGT
CGCAGCCTGCGAGGCCGCCGGCGTCGTCATGGCCACCAACCACCATCTGCGCAACGCGGCCACGCACCGGGCGATCCGCG
ACGTCGTGGCCGGGGGCCGGATCGGGCGCCCGCTTGCCGCGCGGATCGTGCATGGCGGCGCGCTGCCGGCGCATCTGCAT
GGCTGGCGGCTGAAGGACGTATCGGCCGGCGCCGGTGCGATCCTGGACCTGACCGTGCATGATTCCGACCTGCTGCGCTT
CGCGCTGGACGACGAACCGCGCACCGCCATGACGCTGGCGCAGAATGGCGGGCTGGCGCAGCCCGGGATCGAGGACGCGG
CCATGAGCACCATTGCGTTCGGCAGCGGGCTGATCGCCCAGATCTATGAAAGCTTCACGACGCCGTTCCTGCGTACGTCG
TTCGAGGTGCACGGCACGGCCGGGTCGGTGATCGCGCAGGACTGCATGTCACAGACGCCCGGCGGCACGGTCACCCTGCG
GACCGAAGCGGGCGAGGAATCCCTGCCGCTGGAGCAGGAGGATTATTACCAGCGCGGCGTGCGGGCCTTTCATGCCGCGA
TCGCGGGGCAGGGACGGCCGGTGGCCACGGGCGCGGACGGCCTGGTGTCGCTGGCCGTAGCACTCGCCGCGCGCCGTTCC
GCGGAGACAGGCAAGGCCGAGCCGGTCATGCCCTGA

Upstream 100 bases:

>100_bases
AATGCTGAGCGATCACGACGCGCCCTGATCGCCCGCCACGTCCGGACCGGCGCCCGCCGGTCCGGGTGGCCCCTGGAAAA
TCCCCTGAGAGGATCACGTC

Downstream 100 bases:

>100_bases
CAGGATCATGCGCCGGGTCCCGGCGCGGAGGGATGTCGATATGGATTACGCCGAGATGAATAACGAGGCGCTGCGGGCGT
TCAATCAGAAACGCAAGATC

Product: oxidoreductase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTAMRWGLIGATIIGREWMIRAIRDAGGTITSVYSTDAERGRRYAREFDIPDSTTDLDTLLQNVDAVYISTTNDRHHAEC
LAAARAGRHVLCEKPLATRYQDAVEMVAACEAAGVVMATNHHLRNAATHRAIRDVVAGGRIGRPLAARIVHGGALPAHLH
GWRLKDVSAGAGAILDLTVHDSDLLRFALDDEPRTAMTLAQNGGLAQPGIEDAAMSTIAFGSGLIAQIYESFTTPFLRTS
FEVHGTAGSVIAQDCMSQTPGGTVTLRTEAGEESLPLEQEDYYQRGVRAFHAAIAGQGRPVATGADGLVSLAVALAARRS
AETGKAEPVMP

Sequences:

>Translated_331_residues
MTAMRWGLIGATIIGREWMIRAIRDAGGTITSVYSTDAERGRRYAREFDIPDSTTDLDTLLQNVDAVYISTTNDRHHAEC
LAAARAGRHVLCEKPLATRYQDAVEMVAACEAAGVVMATNHHLRNAATHRAIRDVVAGGRIGRPLAARIVHGGALPAHLH
GWRLKDVSAGAGAILDLTVHDSDLLRFALDDEPRTAMTLAQNGGLAQPGIEDAAMSTIAFGSGLIAQIYESFTTPFLRTS
FEVHGTAGSVIAQDCMSQTPGGTVTLRTEAGEESLPLEQEDYYQRGVRAFHAAIAGQGRPVATGADGLVSLAVALAARRS
AETGKAEPVMP
>Mature_330_residues
TAMRWGLIGATIIGREWMIRAIRDAGGTITSVYSTDAERGRRYAREFDIPDSTTDLDTLLQNVDAVYISTTNDRHHAECL
AAARAGRHVLCEKPLATRYQDAVEMVAACEAAGVVMATNHHLRNAATHRAIRDVVAGGRIGRPLAARIVHGGALPAHLHG
WRLKDVSAGAGAILDLTVHDSDLLRFALDDEPRTAMTLAQNGGLAQPGIEDAAMSTIAFGSGLIAQIYESFTTPFLRTSF
EVHGTAGSVIAQDCMSQTPGGTVTLRTEAGEESLPLEQEDYYQRGVRAFHAAIAGQGRPVATGADGLVSLAVALAARRSA
ETGKAEPVMP

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=287, Percent_Identity=23.6933797909408, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87082405, Length=261, Percent_Identity=28.3524904214559, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787574, Length=287, Percent_Identity=25.7839721254355, Blast_Score=65, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24581117, Length=258, Percent_Identity=24.8062015503876, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 35192; Mature: 35061

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAMRWGLIGATIIGREWMIRAIRDAGGTITSVYSTDAERGRRYAREFDIPDSTTDLDTL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHH
LQNVDAVYISTTNDRHHAECLAAARAGRHVLCEKPLATRYQDAVEMVAACEAAGVVMATN
HHCCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCEEEECC
HHLRNAATHRAIRDVVAGGRIGRPLAARIVHGGALPAHLHGWRLKDVSAGAGAILDLTVH
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEC
DSDLLRFALDDEPRTAMTLAQNGGLAQPGIEDAAMSTIAFGSGLIAQIYESFTTPFLRTS
CCHHEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHCC
FEVHGTAGSVIAQDCMSQTPGGTVTLRTEAGEESLPLEQEDYYQRGVRAFHAAIAGQGRP
EEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VATGADGLVSLAVALAARRSAETGKAEPVMP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TAMRWGLIGATIIGREWMIRAIRDAGGTITSVYSTDAERGRRYAREFDIPDSTTDLDTL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHH
LQNVDAVYISTTNDRHHAECLAAARAGRHVLCEKPLATRYQDAVEMVAACEAAGVVMATN
HHCCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCEEEECC
HHLRNAATHRAIRDVVAGGRIGRPLAARIVHGGALPAHLHGWRLKDVSAGAGAILDLTVH
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEC
DSDLLRFALDDEPRTAMTLAQNGGLAQPGIEDAAMSTIAFGSGLIAQIYESFTTPFLRTS
CCHHEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHCC
FEVHGTAGSVIAQDCMSQTPGGTVTLRTEAGEESLPLEQEDYYQRGVRAFHAAIAGQGRP
EEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VATGADGLVSLAVALAARRSAETGKAEPVMP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]