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The map label for this gene is garS [H]

Identifier: 162149288

GI number: 162149288

Start: 3621484

End: 3622722

Strand: Direct

Name: garS [H]

Synonym: GDI_3520

Alternate gene names: 162149288

Gene position: 3621484-3622722 (Clockwise)

Preceding gene: 162149284

Following gene: 162149289

Centisome position: 91.82

GC content: 74.74

Gene sequence:

>1239_bases
ATGGCGGAAGCGATCGCGCGCTCGCCCAGGCTCGAGGCCCTGTTCATCGCCCCCGGCAACCCCGGCACGGCGGCGCTGGG
CACCAACGTCGCCATCGCCGCCGACGACGTACCCGCCCTGGTCGCCCTGGCCCGGGCCGAGCGGATCGACCTGGTGGTGC
CGGGCCCCGAGGCCCCGCTGGTCGCGGGCCTGGCCGACGCCTGCGCCCAGGCCGGCATCCCCTGCGCCGGCCCCAGCCGC
GCGGCGGCGGAACTGGAAGGCAGCAAGAGCTTCACCAAGGAAATCTGCGACGCGGCGTCCATCCCCACCGCGCGGTGGGA
ACGGTTCGACGCCGCCGCCCCGGCGCTGGACTTCGTCCGCCGTCGCGGCGCGCCGATCGTGATCAAGGCCGACGGGCTGG
CGGCCGGCAAGGGCGTCGTCGTGGCCCAGACCGTGGCCGAGGCCGAGGAGGCGATCCGCGCCATGATGGACGACGCCGCC
CTGGGCGAGGCCGGCCGGTCGGTGGTGATCGAGGAATGCCTGACCGGCGACGAAATCTCGCTGTTCGCCTTCTGCGCCGA
CGATACCGCCGTGCTGATCGGCGCGGCGCAGGACCACAAGCGCATCGGCGAGGGCGATACCGGCCCGAACACCGGCGGCA
TGGGCGCGGTGTCACCCCCCGCCGGGTTCGACCGCGCGCGGCAGGAAGCGGCGCTGGACCTGCTGGTCCGCCCGATGCTG
GCCGAGATGGTCCGTCGCGGCACGCCGTTCCGGGGCGTGATCTTCGCCGGGCTGATGCTGACCGAAGACGGCCCCAAGCT
GATCGAATACAATGTCCGCTTCGGCGACCCCGAGGCCCAGGCCCTGCTGATCCGCCTGCAATCCGACCTGCTGCCCGCCC
TGGCCTCGGTCGCGGACGGCACGCTGGACGCGGCGGACATCCGCTTTTCCGACGACCATTCCGTCAGCATCGTGCTGGCC
GCGCGCGGCTATCCCGGCAGGCCCGCCGCCGGCGGCGTGATCGCGGGCCTGGAACAGGCCGGGGCGCTTCCGGGGGTGCA
CGTCTTCCATGCCGGCACCAGGCGCGACGACCAGGGCCGGATCGTGGCGGCGGGCGGGCGCGTGCTGACGGTCTGCGCCA
CCGGCCCCACCCTGGCCCAGGCCCGCGCCCGCGCCTATGACGGCGTGGCCGCCATCCGCTGGGACGACGCGGTCTGGCGC
CGCGACATCGGCGAGCGGGCGCTGCGCGCCTCGTCCTGA

Upstream 100 bases:

>100_bases
GCGTCCCCGGAAGCCTGAGTATGAACCAGGCCGGGCGTGAACTGTTGGAGCGGATGGGATGCGGGTGCTGCTGGTCGGAT
CGGGCGGACGGGAACATGCG

Downstream 100 bases:

>100_bases
GCCACGGCGGGAGAAATTCCCGCCGTCCGGAAACCATCCAGACCAAAACGCGTATTTTCGGATGTGGCTTTCCATTGGGG
GAGTCATATCCGCGTATGCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MAEAIARSPRLEALFIAPGNPGTAALGTNVAIAADDVPALVALARAERIDLVVPGPEAPLVAGLADACAQAGIPCAGPSR
AAAELEGSKSFTKEICDAASIPTARWERFDAAAPALDFVRRRGAPIVIKADGLAAGKGVVVAQTVAEAEEAIRAMMDDAA
LGEAGRSVVIEECLTGDEISLFAFCADDTAVLIGAAQDHKRIGEGDTGPNTGGMGAVSPPAGFDRARQEAALDLLVRPML
AEMVRRGTPFRGVIFAGLMLTEDGPKLIEYNVRFGDPEAQALLIRLQSDLLPALASVADGTLDAADIRFSDDHSVSIVLA
ARGYPGRPAAGGVIAGLEQAGALPGVHVFHAGTRRDDQGRIVAAGGRVLTVCATGPTLAQARARAYDGVAAIRWDDAVWR
RDIGERALRASS

Sequences:

>Translated_412_residues
MAEAIARSPRLEALFIAPGNPGTAALGTNVAIAADDVPALVALARAERIDLVVPGPEAPLVAGLADACAQAGIPCAGPSR
AAAELEGSKSFTKEICDAASIPTARWERFDAAAPALDFVRRRGAPIVIKADGLAAGKGVVVAQTVAEAEEAIRAMMDDAA
LGEAGRSVVIEECLTGDEISLFAFCADDTAVLIGAAQDHKRIGEGDTGPNTGGMGAVSPPAGFDRARQEAALDLLVRPML
AEMVRRGTPFRGVIFAGLMLTEDGPKLIEYNVRFGDPEAQALLIRLQSDLLPALASVADGTLDAADIRFSDDHSVSIVLA
ARGYPGRPAAGGVIAGLEQAGALPGVHVFHAGTRRDDQGRIVAAGGRVLTVCATGPTLAQARARAYDGVAAIRWDDAVWR
RDIGERALRASS
>Mature_411_residues
AEAIARSPRLEALFIAPGNPGTAALGTNVAIAADDVPALVALARAERIDLVVPGPEAPLVAGLADACAQAGIPCAGPSRA
AAELEGSKSFTKEICDAASIPTARWERFDAAAPALDFVRRRGAPIVIKADGLAAGKGVVVAQTVAEAEEAIRAMMDDAAL
GEAGRSVVIEECLTGDEISLFAFCADDTAVLIGAAQDHKRIGEGDTGPNTGGMGAVSPPAGFDRARQEAALDLLVRPMLA
EMVRRGTPFRGVIFAGLMLTEDGPKLIEYNVRFGDPEAQALLIRLQSDLLPALASVADGTLDAADIRFSDDHSVSIVLAA
RGYPGRPAAGGVIAGLEQAGALPGVHVFHAGTRRDDQGRIVAAGGRVLTVCATGPTLAQARARAYDGVAAIRWDDAVWRR
DIGERALRASS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=412, Percent_Identity=42.4757281553398, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI4503915, Length=412, Percent_Identity=42.4757281553398, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI209869995, Length=412, Percent_Identity=42.4757281553398, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI209869993, Length=412, Percent_Identity=42.4757281553398, Blast_Score=320, Evalue=2e-87,
Organism=Escherichia coli, GI1790438, Length=414, Percent_Identity=46.3768115942029, Blast_Score=350, Evalue=9e-98,
Organism=Caenorhabditis elegans, GI17567511, Length=413, Percent_Identity=36.0774818401937, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321203, Length=429, Percent_Identity=39.1608391608392, Blast_Score=282, Evalue=6e-77,
Organism=Drosophila melanogaster, GI24582400, Length=417, Percent_Identity=37.410071942446, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI62471619, Length=414, Percent_Identity=37.43961352657, Blast_Score=245, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 42474; Mature: 42342

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAIARSPRLEALFIAPGNPGTAALGTNVAIAADDVPALVALARAERIDLVVPGPEAPL
CCCCHHCCCCCCEEEEECCCCCCEECCCCEEEECCCHHHHHHHHHHCCEEEEECCCCCCH
VAGLADACAQAGIPCAGPSRAAAELEGSKSFTKEICDAASIPTARWERFDAAAPALDFVR
HHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHH
RRGAPIVIKADGLAAGKGVVVAQTVAEAEEAIRAMMDDAALGEAGRSVVIEECLTGDEIS
HCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEE
LFAFCADDTAVLIGAAQDHKRIGEGDTGPNTGGMGAVSPPAGFDRARQEAALDLLVRPML
EEEEECCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AEMVRRGTPFRGVIFAGLMLTEDGPKLIEYNVRFGDPEAQALLIRLQSDLLPALASVADG
HHHHHCCCCCCHHHHEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
TLDAADIRFSDDHSVSIVLAARGYPGRPAAGGVIAGLEQAGALPGVHVFHAGTRRDDQGR
CCCCEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCC
IVAAGGRVLTVCATGPTLAQARARAYDGVAAIRWDDAVWRRDIGERALRASS
EEEECCEEEEEEECCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEAIARSPRLEALFIAPGNPGTAALGTNVAIAADDVPALVALARAERIDLVVPGPEAPL
CCCHHCCCCCCEEEEECCCCCCEECCCCEEEECCCHHHHHHHHHHCCEEEEECCCCCCH
VAGLADACAQAGIPCAGPSRAAAELEGSKSFTKEICDAASIPTARWERFDAAAPALDFVR
HHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHH
RRGAPIVIKADGLAAGKGVVVAQTVAEAEEAIRAMMDDAALGEAGRSVVIEECLTGDEIS
HCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEE
LFAFCADDTAVLIGAAQDHKRIGEGDTGPNTGGMGAVSPPAGFDRARQEAALDLLVRPML
EEEEECCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AEMVRRGTPFRGVIFAGLMLTEDGPKLIEYNVRFGDPEAQALLIRLQSDLLPALASVADG
HHHHHCCCCCCHHHHEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
TLDAADIRFSDDHSVSIVLAARGYPGRPAAGGVIAGLEQAGALPGVHVFHAGTRRDDQGR
CCCCEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCC
IVAAGGRVLTVCATGPTLAQARARAYDGVAAIRWDDAVWRRDIGERALRASS
EEEECCEEEEEEECCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]